11 results about "Phosphoserine" patented technology

An O-phosphoserine (OPS) export protein variant with increased O-phosphoserine exporting activity, and a method for producing O-phosphoserine, cysteine, and cysteine derivatives using the same. Also provided herein are O-phosphoserine-producing microorganisms, a polynucleotide encoding the O-phosphoserine export protein variant, and a vector containing the polynucleotide encoding the O-phosphoserine export protein variant.

Methods for the fermentative production of O-phosphoserine, cysteate, or taurine in microbes or unicellular organisms that contain a serB mutation that either decreases serB expression, reduces the amount of the serB gene product or results in a serB gene product with low enzymatic activity. Genetic modifications of the O-phosphoserine, cysteate, or taurine and / or substrate biosynthetic pathways in unicellular organisms that include bacteria, algae, microalgae, diatoms, yeast, or fungi are disclosed. Also disclosed are fermentation and processing methods for the production of various O-phosphoserine-, cysteate-, or taurine-containing products and the use of the cells, fermentation broth or extracts that contain O-phosphoserine, cysteate, or taurine to produce products for use in food, feed, beverages, dietary and health supplements, cosmetics, personal care, pharmaceuticals, agricultural production, or surfactants.

The present application relates to a recombinant microorganism in which expression of NADH:quinone oxidoreductase is regulated, and a method for producing O-phosphoserine, cysteine, and cysteine ​​derivatives using the same.

This invention provides a multi-enzyme cascade catalytic reaction system and its application, belonging to the field of enzyme catalysis production technology. Using glycerol as a raw material, it includes a first module, a second module, and a third module. The first module involves the production of glyceric acid from glycerol under the catalysis of alditol oxidase. The second module contains glyceric acid, ATP, and NAD+. + L-glutamic acid is synthesized into phosphoserine via glycerate kinase, D-3-phosphoglycerate dehydrogenase, and phosphoserine transaminase. The third module involves phosphoserine and nucleophiles being catalyzed by phosphoserine thioylase to synthesize L-cysteine ​​or a non-natural L-α-amino acid. The multi-enzyme cascade catalytic reaction system provided by this invention can catalyze a variety of nucleophiles, yields high products, is low in cost, causes minimal environmental pollution, and is easily industrialized.

The present disclosure relates to O-phosphoserine sulfhydrylase variants and methods for producing cysteine using the same.

This application relates to an O-phosphoserine sulfhydrase mutant and a method for producing cysteine ​​using the same.

The present disclosure relates to an O-phosphoserine sulfhydrylase variant and a method for producing cysteine using the same.

A two-component composition including a first component A including water or an aqueous solution, and a second component B including a self-setting adhesive powder including at least a multivalent metal salt and phosphoserine, and at least one monocarbonate selected from the group of sodium carbonate, ammonium carbonate and potassium carbonate, wherein the monocarbonate is present in a concentration between 4 and 12% by weight of component B.

The present disclosure relates to a recombinant microorganism having enhanced ability to produce O-phosphoserine, and a method for producing O-phosphoserine, cysteine, and derivatives thereof using same.

To provide a microorganism for excessively producing O-phosphoserine (hereinafter referred to as "OPS") which is a precursor for producing cysteine in high yield by a method for producing the OPS by fermentation using the microorganism and then reacting the OPS with a sulfide under the catalytic action of O-phosphoserine sulfhydrylase to convert the OPS into L-cysteine.SOLUTION: Provided is a recombinant microorganism of the genus Escherichia producing O-phosphoserine by enhancing the activity of NADH:quinone oxidoreductase.SELECTED DRAWING: None

The invention discloses a free amino acid multi-index combination for identifying wild silver carps, a distinguishing method and application. The free amino acid multi-index combination comprises at least four of phosphoserine, taurine, threonine, sarcosine, beta-aminoisobutyric acid and hydroxyproline. The distinguishing method comprises the following steps: measuring the content of amino acids in a specific combination in a to-be-detected sample, inputting the content into a preset logistic regression model to calculate a wild probability value, and comparing the wild probability value with a distinguishing threshold value optimized and determined through an ROC curve so as to judge a source. According to the technical scheme, the specific biomarker for discriminating the source of the silver carp is defined for the first time, and the problem that a traditional experience discriminating method is high in subjectivity is solved. Through construction of the quantitative model, rapid, objective and high-precision discrimination is realized, and the discrimination accuracy of the optimal combination model can reach 97.8%. The method provides reliable technical support for identifying illegal fished catches in fishery administration law enforcement in the Yangtze River basin, and has important significance for protecting wild silver carp resources.