25 results about "Phosphoserine" patented technology

An O-phosphoserine (OPS) export protein variant with increased O-phosphoserine exporting activity, and a method for producing O-phosphoserine, cysteine, and cysteine derivatives using the same. Also provided herein are O-phosphoserine-producing microorganisms, a polynucleotide encoding the O-phosphoserine export protein variant, and a vector containing the polynucleotide encoding the O-phosphoserine export protein variant.

The present application relates to a milk-derived phosphorylated peptide with the activity of improving intestinal iron absorption and its application in the preparation of iron supplements. The sequence of the milk-derived phosphorylated peptide is EESITRINKKIE, and the glutamic acid at the amino terminal of the sequence is connected with 1-3 phosphoserines. The milk-derived phosphorylated peptide can be combined with Fe 2+ Specifically bound, strong iron chelating ability, and can significantly improve the iron transport amount in the Caco-2 small intestinal epithelial cell model, thereby having good activity of improving intestinal iron absorption, and can be widely applied in the fields of drugs, health care products or food.

The present disclosure relates to a novel YhhS variant and a method for producing O-phosphoserine, cysteine and a derivative of cysteine using the same.

The present disclosure relates to a recombinant microorganism in which the activity of a protein having manganese import activity is regulated, and a method for producing O-phosphoserine, cysteine or a cysteine derivative using the microorganism.

The present disclosure relates to a recombinant microorganism having an increased O-phosphoserine production capacity, and a method for producing O-phosphoserine, cysteine, and cysteine derivatives using the same.

Methods for the fermentative production of O-phosphoserine, cysteate, or taurine in microbes or unicellular organisms that contain a serB mutation that either decreases serB expression, reduces the amount of the serB gene product or results in a serB gene product with low enzymatic activity. Genetic modifications of the O-phosphoserine, cysteate, or taurine and / or substrate biosynthetic pathways in unicellular organisms that include bacteria, algae, microalgae, diatoms, yeast, or fungi are disclosed. Also disclosed are fermentation and processing methods for the production of various O-phosphoserine-, cysteate-, or taurine-containing products and the use of the cells, fermentation broth or extracts that contain O-phosphoserine, cysteate, or taurine to produce products for use in food, feed, beverages, dietary and health supplements, cosmetics, personal care, pharmaceuticals, agricultural production, or surfactants.

The present application relates to a recombinant microorganism in which expression of NADH:quinone oxidoreductase is regulated, and a method for producing O-phosphoserine, cysteine, and cysteine ​​derivatives using the same.

The present disclosure relates to a novel YhhS variant and a method for producing O-phosphoserine, cysteine and a derivative of cysteine using the same.

Compositions and methods for inducing immune tolerance to proteins and subsequence administration of the proteins are disclosed. Compositions comprise proteins complexed with liposomes comprising PS and PC, wherein at least part of the PS is present as lyso-PS.

Methods for the fermentative production of O-phosphoserine, cysteate, or taurine in microbes or unicellular organisms that contain a serB mutation that either decreases serB expression, reduces the amount of the serB gene product or results in a serB gene product with low enzymatic activity. Genetic modifications of the O-phosphoserine, cysteate, or taurine and / or substrate biosynthetic pathways in unicellular organisms that include bacteria, algae, microalgae, diatoms, yeast, or fungi are disclosed. Also disclosed are fermentation and processing methods for the production of various O-phosphoserine-, cysteate-, or taurine-containing products and the use of the cells, fermentation broth or extracts that contain O-phosphoserine, cysteate, or taurine to produce products for use in food, feed, beverages, dietary and health supplements, cosmetics, personal care, pharmaceuticals, agricultural production, or surfactants.

The application discloses an anti-pollution electrochemical serum sensor based on a phosphoric acid group modified polypeptide, a preparation method and application, and belongs to the technical field of electrochemical detection. A phosphoric acid group is introduced into a polyhydroxy proline polypeptide sequence. The polyhydroxy proline region of the polypeptide has excellent hydrophilicity, can form a hydration layer, and inhibits the adsorption of pollutants through steric hindrance and energy barrier; the terminal contains two phosphoserine branches, and the phosphoric acid group can be stably connected with phytic acid molecules on the substrate through the bridging action of vanadium ions. The sensing interface constructed by the ion coordination method is not affected by biological thiols, and the strong hydrophilicity of phytic acid and the polypeptide endows the interface with excellent anti-pollution ability. The precise detection of three biomarkers (CA15-3, CEA and AFP) can be realized in serum. The comprehensive analysis of the three markers combined with machine learning evaluation can improve the accuracy of early diagnosis of breast cancer.

This invention provides a multi-enzyme cascade catalytic reaction system and its application, belonging to the field of enzyme catalysis production technology. Using glycerol as a raw material, it includes a first module, a second module, and a third module. The first module involves the production of glyceric acid from glycerol under the catalysis of alditol oxidase. The second module contains glyceric acid, ATP, and NAD+. + L-glutamic acid is synthesized into phosphoserine via glycerate kinase, D-3-phosphoglycerate dehydrogenase, and phosphoserine transaminase. The third module involves phosphoserine and nucleophiles being catalyzed by phosphoserine thioylase to synthesize L-cysteine ​​or a non-natural L-α-amino acid. The multi-enzyme cascade catalytic reaction system provided by this invention can catalyze a variety of nucleophiles, yields high products, is low in cost, causes minimal environmental pollution, and is easily industrialized.

The present disclosure relates to O-phosphoserine sulfhydrylase variants and methods for producing cysteine using the same.

This application relates to an O-phosphoserine sulfhydrase mutant and a method for producing cysteine ​​using the same.

The present disclosure relates to an O-phosphoserine sulfhydrylase variant and a method for producing cysteine using the same.

To provide a method to overproduce its precursor, O-phosphoserine, in order to produce high yield of cysteine.SOLUTION: The present application relates to a novel MdtH variant and a method for producing O-phosphoserine, cysteine, and a derivative of cysteine using the same.SELECTED DRAWING: None

The present application relates to novel YhhS mutants and methods for producing O-phosphoserine, cysteine ​​and cysteine ​​derivatives using the same.

The present application relates to novel MdtH mutants and methods for producing O-phosphoserine, cysteine, and cysteine ​​derivatives using the same.

A two-component composition including a first component A including water or an aqueous solution, and a second component B including a self-setting adhesive powder including at least a multivalent metal salt and phosphoserine, and at least one monocarbonate selected from the group of sodium carbonate, ammonium carbonate and potassium carbonate, wherein the monocarbonate is present in a concentration between 4 and 12% by weight of component B.

The present disclosure relates to a recombinant microorganism having enhanced ability to produce O-phosphoserine, and a method for producing O-phosphoserine, cysteine, and derivatives thereof using same.

The present disclosure generally relates to a composition for use in a therapeutic method of treatment or prevention of a retinal disease or retinal impairment, said composition comprising: A) carnitine, deoxycarnitine, acetyl-L-carnitine, gamma-butyrobetaine, 4- trimethylammoniobutanal, 3-hydroxy-N6,N6,N6-trimethyl-L-lysine, N6,N6,N6- trimethyl-L-lysine and / or lysine, preferably carnitine, such as L-carnitine; B) nicotinamide, nicotinamide riboside, quinolinate, deamino-NAD+, nicotinate D- ribonucleotide, nicotinamide D-ribonucleotide, nicotinate D-ribonucleoside, and / or nicotinate; C) optionally serine, glycine, betaine, N-acetylglycine, N-acetylserine, dimethylglycine, sarcosine and / or phosphoserine, preferably serine or glycine; and D) optionally N-acetyl cysteine, cysteine and / or cystine.

The present disclosure relates to a recombinant microorganism in which the activity of an alkyl hydroperoxide reductase is modulated, and a method for producing O-phosphoserine, cysteine, and cysteine derivatives using the microorganism.

The invention discloses a strain for producing taurine by a one-step method and a construction method thereof, and belongs to the field of gene engineering. Corynebacterium glutamicum ATCC13032 is used as a chassis bacterium, by weakening gpmA gene expression in a TCA path, knocking out serB and ldh genes in L-serer and acetic acid-lactic acid synthesis paths and enhancing serA and serC gene overexpression in a phosphoserine synthesis path, a genetically engineered bacterium for efficiently synthesizing taurine by taking glucose as a substrate is successfully constructed, and the yield of the strain is up to 1.5 g / L after the strain is fermented for 96 hours in a shake flask. The taurine yield of the genetically engineered bacterium is further improved by optimizing a fermentation culture medium, and the yield reaches 1.8 g / L.

To provide a microorganism for excessively producing O-phosphoserine (hereinafter referred to as "OPS") which is a precursor for producing cysteine in high yield by a method for producing the OPS by fermentation using the microorganism and then reacting the OPS with a sulfide under the catalytic action of O-phosphoserine sulfhydrylase to convert the OPS into L-cysteine.SOLUTION: Provided is a recombinant microorganism of the genus Escherichia producing O-phosphoserine by enhancing the activity of NADH:quinone oxidoreductase.SELECTED DRAWING: None

The invention discloses a free amino acid multi-index combination for identifying wild silver carps, a distinguishing method and application. The free amino acid multi-index combination comprises at least four of phosphoserine, taurine, threonine, sarcosine, beta-aminoisobutyric acid and hydroxyproline. The distinguishing method comprises the following steps: measuring the content of amino acids in a specific combination in a to-be-detected sample, inputting the content into a preset logistic regression model to calculate a wild probability value, and comparing the wild probability value with a distinguishing threshold value optimized and determined through an ROC curve so as to judge a source. According to the technical scheme, the specific biomarker for discriminating the source of the silver carp is defined for the first time, and the problem that a traditional experience discriminating method is high in subjectivity is solved. Through construction of the quantitative model, rapid, objective and high-precision discrimination is realized, and the discrimination accuracy of the optimal combination model can reach 97.8%. The method provides reliable technical support for identifying illegal fished catches in fishery administration law enforcement in the Yangtze River basin, and has important significance for protecting wild silver carp resources.