Casein phosphopeptide and preparation method and application thereof

A casein phosphopeptide and casein technology, which is applied in the field of casein phosphopeptide and its preparation, can solve problems such as inability to standardize product quality, inaccurate evaluation of drug efficacy, determination of casein phosphopeptide content, etc., to promote absorption and utilization , low cost and simple process

Active Publication Date: 2019-11-22
INFINITUS (CHINA) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the content of casein phosphopeptides has not been determined, so the quality of the product cannot be standardized, and its efficacy cannot be accurately evaluated.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0054] The present invention also provides the above milk high activity casein phosphopeptide and a preparation method thereof, comprising the following steps:

[0055] Weigh casein raw materials respectively, put them into tank 1 and tank 2, add water and stir to dissolve, add 10% sodium hydroxide to dissolve;

[0056] (1) After the pH in tank 1 is stable, add trypsin for enzymatic hydrolysis;

[0057] (2) After the pH is stabilized in tank 2, add the compound protease enzymolysis of trypsin and neutral protease;

[0058] (3) Combine the enzymolysis solutions obtained in step (1) and step (2), adjust the pH to 4.0-6.0, adjust the temperature to 80°C-90°C, keep warm for 20min-30min, and sterilize the enzyme; cool to 50°C Within, the suspension enzymatic hydrolyzate was obtained.

[0059] (4) Filtrate the suspension enzymatic hydrolyzate obtained in (3) with a centrifuge or a plate and frame filter to obtain a filtrate.

[0060] (5) Separation and purification: adjust the pH...

Embodiment 1

[0075] Weigh casein raw materials respectively, put them into tank 1 and tank 2, add water and stir to dissolve, add 10% sodium hydroxide to dissolve; wherein, the ratio of casein in tank 1 and tank 2 is 3:1, and the ratio of material to water when dissolving is 1 :10, the reaction temperature is 50°C, and the pH is controlled to be pH8.0.

[0076] (1) After the pH in tank 1 is stable, add trypsin for enzymatic hydrolysis;

[0077] Wherein, the amount of enzyme added is 0.3% of the mass of casein, the pH is controlled at 8.0, the reaction temperature is 50° C., and the enzymolysis time is 2.0 h.

[0078] (2) After the pH is stabilized in the tank 2, add trypsin and neutral protease for enzymatic hydrolysis; wherein, the amount of enzyme added is 0.25% of the casein quality, and the ratio of trypsin and neutral protease is 9:1. The pH is controlled at 8.0, the temperature is controlled at 50°C, and the enzymatic hydrolysis time is 1.0h.

[0079] (3) Combine the enzymolysis so...

Embodiment 2

[0086] Weigh casein raw materials respectively, put them into tank 1 and tank 2, add water and stir to dissolve, add 10% sodium hydroxide to dissolve;

[0087] Among them, the ratio of casein in tank 1 and tank 2 is 1:1, the ratio of material to water during dissolution is 1:8, the dissolution temperature is 40°C, and the pH is controlled at pH 7.5.

[0088] (1) After the pH in tank 1 is stable, add trypsin for enzymatic hydrolysis;

[0089] Wherein, the amount of enzyme added is 0.1% of the mass of casein, dissolved by adding 10% sodium hydroxide, the pH is controlled at 8.5, the temperature is controlled at 40° C., and the enzymolysis time is 2.5 hours.

[0090] (2) After the pH is stabilized in tank 2, add the compound protease enzymolysis of trypsin and neutral protease;

[0091] Wherein, the amount of enzyme added is 0.1%- of the mass of casein, and the ratio of trypsin to neutral protease is 8:1. Add 10% sodium hydroxide to dissolve, control the pH to 8.5, control the ...

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Abstract

The invention relates to the field of polypeptides, in particular to casein phosphopeptide and its preparation method and application. In the invention, casein in cow's milk is used as a raw material.Casein phosphopeptide with strong calcium holding activity is prepared through trypsin and compound protease (trypsin and neutral protease) enzymolysis, enzyme inactivation, centrifugation, adjustment of isoelectric point/alcohol precipitation and impurity removal, and drying processes. The special cluster phosphoserine structure obtained by the invention has the ability of chelating calcium, andcan effectively promote the absorption and utilization of calcium. The molecular weight of the milk-derived casein phosphopeptide prepared by the invention is mainly between 1,000 and 5,000, which isbeneficial to human body absorption. The content of casein phosphopeptide is more than 85%, and its yield can reach 25%. The method is a preparation method with low cost and simple process, and is suitable for industrial production.

Description

technical field [0001] The invention relates to the field of polypeptides, in particular to casein phosphopeptides and their preparation methods and applications. Background technique [0002] Casein phosphopeptide (CPP) is made from cow's milk casein through biotechnology. It has peptides that promote the absorption and utilization of divalent metal ions such as calcium, iron, and zinc. It can be used in various nutritional and health foods. Casein phosphopeptide is a polypeptide hydrolyzed by trypsin and neutral protease, which is refined and purified. Its core structure is: -Ser(P)-Ser(P)-Ser(P)-Glu-Glu-(Ser : Serine, Glu: Glutamic acid, P: Phosphate group). Phosphoserine residues (-Ser(P)-) in this structure exist in clusters, which are negatively charged in a weakly neutral intestinal pH environment, which can prevent further action of digestive enzymes and prevent casein phosphopeptides from being further hydrolysis. Casein phosphopeptide can combine with calcium in...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K7/08C12P21/06C07K14/47A23L33/18A61K38/10A61P3/14
CPCA23V2002/00A61K38/00A23L33/18A61P3/14C07K7/08C07K14/4732C12P21/06A23V2200/30A23V2250/55
Inventor 罗珍夏祖猛陈媛媛罗琥捷李文治
Owner INFINITUS (CHINA) CO LTD
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