15 results about "Zymosan" patented technology

The invention discloses a method for differentiating induced pluripotent stem cells into macrophages. The method comprises the following steps: S1, inducing pluripotent stem cells to form embryoid bodies; s2, differentiating the embryoid body into a monocyte with CD14 expression; s3, the mononuclear cells are differentiated into macrophages with CD14 / CD11b expression; and S4, polarizing the macrophages into M1 type macrophages and / or M2 type macrophages. According to the method for differentiating the induced pluripotent stem cells into the macrophages, the cells can be effectively induced to differentiate towards the macrophages, the induced pluripotent stem cells are subjected to directional differentiation culture in a short time to obtain the macrophages, and the generated macrophages express CD14 and CD11b and have remarkable phagocytic activity on yeast glycan. The method is of great significance to large-scale production of macrophages.

The invention discloses a yeast beta-glucan preparation method based on high pressure-microjet homogenization treatment, and belongs to the technical field of zymosan extraction. The method comprises the following steps: carrying out high pressure-microjet homogenization pretreatment on active dry yeast, then carrying out autolysis treatment, then extracting beta-glucan by an acid-alkali method, and finally carrying out freeze drying to obtain a high-purity product. The core of the method is that the yeast cell walls are subjected to physical wall breaking by adopting a high pressure-microjet homogenization technology, and the extraction rate and the purity of the beta-glucan are remarkably improved by combining with an optimized acid-alkali treatment process. Compared with a traditional enzymolysis method, the method has the advantages that damage of exogenous enzyme to the beta-glucan structure is avoided, the purity of the obtained product reaches 84% or above, the content of protein impurities is lower than 0.2%, a new way is provided for efficient utilization of yeast cell wall resources, and the method is suitable for the fields of food, medicine and health care products.

The present invention provides extracellular vesicles isolated from stem cells treated with zymosan, Poly LC, and monophosphoryl lipid A (MPLA), their use for treatment of cancer, and a method for preparing the same. The extracellular vesicles according to the present invention contain various miRNAs and cytokines related to anticancer effects, and have tumor growth inhibitory effects. In addition, since the extracellular vesicles exhibit a synergistic effect in cancer treatment when administered in combination with an immune checkpoint inhibitor, a pharmaceutical composition for cancer treatment containing the extracellular vesicles as an active ingredient is highly likely to be effectively useful in cancer treatment by increasing immune activity in the body.

The invention relates to a composition for improving the immunity of a human body and application of the composition, and relates to the technical field of medicines and health care products. , 50-80 parts of rabdosia serra, 15-35 parts of burdock root, 5-10 parts of zymosan and 10-20 parts of clinacanthus nutans. The composition provided by the invention takes rabdosia lingua as a core component and is matched with a proper amount of burdock root, clinacanthus nutans and the like, and all the components cooperate with one another, so that the composition has a remarkable effect of improving immunity. In addition, the selected raw materials are derived from the idea of homology of medicine and food, so that the safety and acceptability of the composition are ensured. Due to the characteristic, the compound is not only suitable for developing treatment medicines for specific people with low immunity, but also widely suitable for preparing various health-care products, such as dietary supplements and functional foods, so that the diversified requirements of different consumer groups on healthy life are met, and the compound has a wide application range.

Compositions and methods for treating cancer, particularly leukemia, using a cytotoxic composition comprising monocytes activated by β-glucan. The monocytes are preferably incubated with the β-glucan and then processed to extract particles, such as microvesicles and exosomes from the treated monocytes to produce the cytotoxic composition. Preferably the cytotoxic composition comprises at least 50% exosomes having a size of 150 nm or less that are activated with β-glucan. Zymosan is the preferred β-glucan. The cytotoxic composition has an apoptosis effect. When a subject having cancer is treated according to preferred embodiments, the cytotoxic composition preferably induces a cytokine response in the subject's immune system. The combination of the cytotoxic composition and cytokine response are synergistic.

The invention provides a gel product which comprises a gel agent and zymosan, and the zymosan is selected from yeast beta-glucan. The gel product provided by the invention comprises the yeast beta-glucan, so that the chewiness, the elasticity and the hardness of the soft sweet product can be remarkably improved, the viscosity is reduced, the texture property of the soft sweet is improved, and the mouth feel is improved; meanwhile, the structural stability of jelly products can be improved, the jelly products are not prone to being dispersed and broken under the action of external force, the adhesiveness is reduced, and the texture and taste are improved. The yeast beta-glucan is added into the gel product, so that nutrient substances in the product can be enriched, and zymosan has the effect of improving body immunity and has a good prevention effect on upper respiratory tract infection. The preparation method has the advantages of simple operation, no special requirement, no influence on flavor, and high application value.

The invention discloses a preparation method of tea-aroma yellow rice wine, and belongs to the technical field of wine brewing. The method comprises the following steps: respectively crushing white hyacinth bean flower, fructus piperis longi and tea leaves; glutinous rice and lactic acid bacteria are added into water for rice soaking; steaming the rice, cooling the glutinous rice to 50 DEG C, adding the white hyacinth bean flower powder, and continuously cooling to 35 DEG C; putting materials into a vat, namely uniformly stirring and mixing water, a white hyacinth bean flower-rice mixture, wheat koji, distiller's yeast and cellulase; performing primary fermentation after feeding, during the primary fermentation period, performing primary raking 8-12h after primary fermentation, then performing timed raking, performing primary fermentation for four times, and adding zymosan for fermentation during third raking; cooling the fermented mash after pre-fermentation, performing post-fermentation, and adding tea powder and fructus piperis longi powder in stages during the post-fermentation period; and after post-fermentation, squeezing, centrifuging and sterilizing the fermentation liquor to obtain the tea-aroma yellow wine. The yellow rice wine disclosed by the invention is sweet and mellow in vinosity, pleasant in fragrance and good in mouth feel, and has tea fragrance, and the bioavailability and flavor quality of theaflavin in the tea-fragrance yellow rice wine are remarkably improved.

Compositions and methods for treating cancer, particularly leukemia, using a cytotoxic composition comprising monocytes activated by-glucan. The monocytes are preferably incubated with the β-glucan and then processed to extract particles, such as microvesicles and exosomes from the treated monocytes to produce the cytotoxic composition. Preferably the cytotoxic composition comprises at least 50% exosomes having a size of 150 nm or less that are activated with β-glucan. Zymosan is the preferred β-glucan. The cytotoxic composition has an apoptosis effect. When a subject having cancer is treated according to preferred embodiments, the cytotoxic composition preferably induces a cytokine response in the subject's immune system. The combination of the cytotoxic composition and cytokine response are synergistic.

The invention relates to the technical field of quality detection of traditional Chinese medicine, and particularly discloses a detection method of a traditional Chinese medicine composition for repairing kidney injury and the traditional Chinese medicine composition.The traditional Chinese medicine composition is prepared by matching poria cocos, astragalus membranaceus, American ginseng and cordyceps sobolifera water extracts according to a specific proportion and being supplemented with zymosan and water-soluble vitamin E. According to the detection method, a high-sensitivity CMOS image sensor is adopted to collect a two-dimensional optical image when components in an HPLC system flow out, and the visibility of a low-concentration signal is improved by combining local contrast enhancement, frequency domain filtering and RO I segmentation; an overlapped peak region is identified through a customized lightweight convolutional neural network model, and the identification precision is improved by fusing multi-scale features, an attention mechanism and a knowledge distillation strategy; constructing an image texture and ultraviolet spectrum multi-modal fusion analysis matrix, and combining principal component analysis and component specificity weight factors to realize accurate quantification; and finally outputting the annotated image and the structured quality report.

The invention belongs to the technical field of construction of disease animal models, and discloses a construction method of a psoriatic arthritis animal model, which adopts zymosan mannan and a complete Freund's adjuvant to jointly induce psoriatic arthritis for modeling. According to the invention, the zymosan mannan and the complete Freund's adjuvant are used for combined induction, so that the typical pathological manifestation of psoriatic arthritis can be stably simulated, and particularly, the model is superior to the existing animal model in the aspect of simultaneously inducing joint inflammation and psoriasis-like skin lesion, and is closer to the pathological characteristics of clinical patients.

The present invention relates to compositions and methods for treating cancer, particularly leukemia, using a cytotoxic composition comprising monocytes activated by β-glucan. The monocytes are preferably incubated with β-glucan and then treated to extract particles, such as microvesicles and exosomes, from the treated monocytes to produce the cytotoxic composition. Preferably, the cytotoxic composition contains at least 50% β-glucan-activated exosomes having a size of 150 nm or less. Zymosan is a preferred β-glucan. The cytotoxic composition has an apoptotic effect. When a subject with cancer is treated according to the preferred embodiment, the cytotoxic composition preferably induces a cytokine response in the subject's immune system. The combination of the cytotoxic composition and the cytokine response is synergistic.

This invention discloses a composition containing myxobin and zinc hyaluronic acid and its application. The present invention combines myxobin and zinc hyaluronic acid in a specific ratio, which, when used alone, has a better effect on reducing DHT damage to hair follicles and also better promotes hair follicle angiogenesis, exhibiting a synergistic effect on hair growth and hair loss prevention.

The present invention provides extracellular vesicles isolated from stem cells treated with zymosan, Poly I:C, and monophosphoryl lipid A (MPLA), their use for treatment of cancer, and a method for preparing the same. The extracellular vesicles according to the present invention contain various miRNAs and cytokines related to anticancer effects, and have tumor growth inhibitory effects. In addition, since the extracellular vesicles exhibit a synergistic effect in cancer treatment when administered in combination with an immune checkpoint inhibitor, a pharmaceutical composition for cancer treatment containing the extracellular vesicles as an active ingredient is highly likely to be effectively useful in cancer treatment by increasing immune activity in the body.

A composition containing yeast protein, a preparation method therefor, and the use of the yeast protein and the composition. The outer layer of the yeast protein is coated with zymosan; the yeast protein is in the shape of a sphere or an ellipsoid; the yeast protein has a protein content of 70% or more and a zymosan content of 5%-30%, calculated as the weight percentage of a dry base. The yeast protein of the present invention has obviously lower digestibility than that of soybean protein isolate and whey protein in the stomach, causes a stronger satiety after being eaten compared with whey protein, has the characteristic of slow digestion, and is a slow-digesting protein. The yeast protein of the present invention also contains 18 amino acids, including eight essential amino acids and two semi-essential amino acids for the human body, and thus is a complete protein due to the full type of amino acids. The yeast protein can be used as the slow-digesting protein in the preparation of a composition which is intended to be enterally administered to mammals so as to regulate the postprandial amino acid level in the blood plasma.

The present application relates to the field of food or medicine technology, and in particular to a zymopolysaccharide soft capsule shell, a preparation method and application thereof. The shell can effectively improve the stability problems of the soft capsule product, such as adhesion, oil leakage and hardening of the shell, which are prone to occur during the shelf life, and has a more excellent protective effect on the active ingredients of the environment-sensitive content. At the same time, the raw material cost is low, the preparation process is simple, and the shell has a broad application prospect in the fields of food, medicine and cosmetics.