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275 results about "Theaflavin" patented technology

Theaflavin (TF) and its derivatives, known collectively as theaflavins, are antioxidant polyphenols that are formed from the condensation of flavan-3-ols in tea leaves during the enzymatic oxidation (sometimes erroneously referred to as fermentation) of black tea. Theaflavin-3-gallate, theaflavin-3'-gallate, and theaflavin-3-3'-digallate are the main theaflavins. Theaflavins are types of thearubigins, and are therefore reddish in color. Epigallocatechin gallate (EGCG) will metabolize into some theaflavins in the liver. Those molecules contain a tropolone moiety.

Pu'er tea processing method by inoculated fungi microbe

The invention provides a processing method of pu-er tea with inoculated fungi microorganism. During the first day of pu-er tea pile-fermentation, aspergillus niger red koji and penicillum sp. red koji is mixed together by the ratio of 1-2:1 and is added into the production process of pu-er tea by the weight percentage of uniform mixed red koji and basic tea of 1:125-250; during the second day of pu-er tea pile-fermentation, saccharomyces sp. is added into the production process of pu-er tea by the weight percentage of saccharomyces sp. and basic tea of 1:250-500 and then material is gathered together and covered with fermentation cloth; fermentation gathering temperature is controlled between 40 celsius degree and 65 celsius degree and gathering overturning has to be carried on apporiately to control moisture content between 14 percent and 20 percent; when tea leaves moisture content is over than 20 percent, ditch recanalization has to be carried on every day; when tea leaves moisture content is 14 percent to 20 percent, ditch recanalization has to be carried on every three days; with shortened processing time, rich water extract, proper tea polyphenol and amino acids is retained and a certain amount of caffeine, hydration pectin, theaflavins and theabrownin is added; compared with natural pile-fermentation, the quality of the invention is superior and more stable and microbial indicator is equal to that of red tea and green tea.
Owner:TEA INST YUNNAN ACADEMY OF AGRI SCI

Processing method of high-theaflavin black tea beverage

The invention relates to a processing method of a high-theaflavin black tea beverage, and belongs to the technical field of the processing method of the black tea beverage. The processing method of the high-theaflavin black tea beverage comprises the following steps of: 1) adding hot water to dried green tea for dynamic extraction, and then filtering tea grounds to obtain a tea soup; 2) cooling the tea soup, adding a fresh fruit syrup for aerobic stirring fermentation, filtering pomace to obtain a fermented high-theaflavin black tea juice; 3) the fermented high-theaflavin black tea juice sequentially being subjected to centrifugal separation and microfiltration membrance filtering to obtain a clarified high-theaflavin black tea juice; and 4) preparing, sterilizing and canning the clarified high-theaflavin black tea juice according to the concentration ratio required by the black tea beverage to obtain the high-theaflavin black tea beverage. The total amounts of flavor chemical substances tea polyphenols and amino acids in the black tea beverage obtained from the invention are respectively up to more than 500mg / L and more than 65mg / L, the soup is red brilliant and bright, the tea aroma is full-bodied, the flavor is fruity and hi-fresh, the tea tastes fresh, brisk and good, and the tea pigment content is high, wherein the theaflavin content can reach more than 80mg / L.
Owner:TEA RES INST CHINESE ACAD OF AGRI SCI

Method for preparing theaflavin by dynamic continuous oxidation on immobilized polyphenol oxidase

The invention discloses a method for preparing theaflavin through carrying out dynamic continuous oxidation on immobilized polyphenol oxidase. in the method, tea polyphenols is taken as a raw material. At present, the content of theaflavin only accounts for 0.3-1.5% of dry matter of black tea, and the yield is low because the black tea is directly used for extracting. The synthesis and preparation technology of the existing theaflavin can not be dynamically and continuously prepared and is only confined in the laboratory to be prepared, thereby causing higher theaflavin cost and confining the application of theaflavin in medical heath care. In the method, tea polyphenol serves as a substrate, and immobilized polyphenol oxidase serves as a catalyst medium. Reaction temperature is controlled. The substrate and oxygen continuously enter on a packed bed reactor to oxidize tea polyphenol and polymerize to form theaflavin substance, and the theaflavin substance is continuously pumped out by a constant flow pump. In the method, the immobilized polyphenol oxidase is repeatedly utilized on a bioreactor to continuously and dynamically prepare the theaflavin to obtain the theaflavin mixed liquor. The method provides a basis for industrial preparation of theaflavin.
Owner:TEA RES INST CHINESE ACAD OF AGRI SCI

Method for preparing high-purity theaflavin composite

The invention provides a method for preparing a high-purity theaflavin composite. The method comprises the following steps of: adding a mixture of green tea and catechinic acid into a crude polyphenol oxidase liquid for carrying out in-vitro oxidation; then, extracting the mixture with ethyl acetate; concentrating the extract in vacuum and drying to obtain a theaflavin crude product; dissolving the theaflavin crude product with ethanol water with volume concentration being 20% and then absorbing by using a chromatographic column with absorbing resin till the chromatographic column with absorbing resin is in a saturated state; eluting by using the ethanol water with volume concentration being 20%, and then eluting by using acetone or ethanol water with volume concentration being 60%; concentrating the eluting liquid, namely the collected acetone or ethanol water solution with volume concentration being 60% in vacuum; and obtaining the high-purity theaflavin composite through freeze drying. The method provided by the invention has the advantages of simple process and low cost and avoids using toxic and harmful organic reagents during a production process, thereby being environment-friendly. Furthermore, the high-purity theaflavin composite with different theaflavin components can be obtained by controlling different reaction conditions, so that the product yield is high and the content is high.
Owner:HUNAN AGRICULTURAL UNIV

Method for producing theaflavin by fermenting by adopting phase transfer enzyme catalysis technology

InactiveCN101979632AAvoid loss of catalysisSimple processFermentationFreeze-dryingOxygen
The invention relates to a method for extracting tea polyphenol by adopting phase transfer technology. The method is characterized by comprising the following steps of: cutting and crushing fresh tea leaves serving as an enzyme source; adding dry tea leaves, a phase transfer agent and an extracting agent serving as raw materials for extracting the tea polyphenol; blasting air or oxygen while stirring at room temperature; separating fermentation liquor; repeatedly adding the extracting agent for performing secondary fermentation and thrice fermentation; merging the fermentation liquor; concentrating under a reduced pressure until the fermentation liquor is nearly dry; adding water into concentrated solution; continuing concentrating under the reduced pressure and desolventizing to obtain the aqueous solution of theaflavin; centrifuging and filtering precipitated chlorophyll; and performing spray-drying, vacuum-drying or freeze-drying on the aqueous solution of the theaflavin from which the chlorophyll is removed to obtain solid theaflavin. In the method, the process is simple; all raw materials are natural green agricultural and sideline products; the process saves energy and environmental-friendly; a process production does not produce process spent water; equipment investment is simple; and production cost is low.
Owner:MAITE SHANGHAI BIOLOGICAL TECH

Method for processing yellow tea of Guire #2 tea trees

The invention provides a method for processing yellow tea of Guire #2 tea trees. The method comprises the following steps: (1) selecting materials; (2) performing withering; (3) performing deactivation; (4) performing primary yellowing heaping; (5) performing kneading; (6) performing shaping and strip molding; (7) performing primary drying; (8) performing secondary yellowing heaping; (9) performing re-drying; (10) enhancing fragrance. By adopting the method, both primary yellowing heaping and secondary yellowing heaping are carried out with plastic cloth, and as the plastic cloth is thin and breathable, enzymes and theaflavin can be rapidly converted, the time can be shortened, and the cost can be reduced; in the kneading process, slight kneading is carried out, tea leaves are mutually abraded, leaf margin cells are broken, enzyme oxidation promotion is accelerated, tea leaf moisture loss is promoted, effective substances in water and leaves are combined and converted into relatively thick fragrance substances, meanwhile due to moisture evaporation and transferring, fragrance and taste can be well converted, relatively high-quality tea leaves can be processed, and furthermore an obtained tea leaf product is compact and straight in appearance, tidy and unified in length, thick and pure in fragrance and fresh, mellow and smooth in taste.
Owner:SOUTH ASIAN TROPICAL AGRI SCI RES INST OF GUANGXI

Preparation method of theaflavin enteric microcapsule, as well as product prepared by preparation method and application of product

ActiveCN103251572AImprove the poor stability of dosage form, low utilization rate and other defectsReasonable stepsOrganic active ingredientsMetabolism disorderTheaflavinEmulsion
The invention discloses a theaflavin enteric microcapsule particle, a preparation method and an application thereof. The preparation method comprises the following steps of: (1) dissolving 2-8 parts by weight of a capsule material, which is an enteric material, into a 80%-95% ethanol water solution to prepare 1%-8% capsule material solution, dissolving 0.5-2 parts by weight of theaflavin-containing tea extract into distilled water to obtain 0.15%-2% core material solution, adding 2%-5% of stearic acid into the core material solution, and stirring and emulsifying to obtain a core material emulsion; and (2) mixing the capsule material solution and the core material emulsion, uniformly stirring, adding 0.01-0.1 part by weight of an antisticking agent and 1-8 parts by weight of castor oil and/or span-80 into a mixture, stirring and emulsifying to obtain an emulsion, and drying the emulsion, wherein the weight ratio of the core material to the capsule material is 1:2 to 1:8. A theaflavin enteric microcapsule disclosed by the invention is simple in preparation method and process, good in stability, convenient to store and transport, uniform in granularity, good in dispersibility, high in medicament encapsulation rate and dissolubility and capable of being widely applied to the fields, such as health-care products, medicaments and the like.
Owner:SHANGHAI KE BAO BIOTECH CO LTD

Tea polyphenol oxidase isozyme monomers PP01 and PP02 and preparation method thereof

The invention discloses tea polyphenol oxidase isozyme monomers PP01 and PP02 and a preparation method thereof. With respect to the technical states that existing tea polyphenol oxidase is low in purification factor, low in loading quantity of protein samples and long in operation time, the obtained isozyme is the isozyme with one relative molecular weight, and the isozyme monomer with specific amino acid sequence structure, molecular weight, isoelectric point, enzymatic property and the like cannot be obtained, two tea polyphenol oxidase isozyme monomers with specific specific amino acid sequence structures, molecular weight, isoelectric points, enzymatic properties and the like are separated and prepared by sequentially adopting the technologies and optimization processes such as enrichment of zymoprotein with acetone powder, fractional precipitation of ammonium sulfate, strong anion exchange chromatography, gel medium chromatography, molecular identification and enzymatic property analysis. The method has the advantages of low cost, large preparation amount, high pertinence, short operation cycle and the like, and is of important theoretical and practical significance on deepening of the separation and purification technology of tea polyphenol oxidase isozyme, promoting of directional enzymatic synthesis of a theaflavin component by virtue of the tea polyphenol oxidase isozyme monomers, development and utilization of advantaged isozyme of tea polyphenol oxidase, isozyme immobilization, enzyme protein structure analysis, enzyme gene cloning, functional verification and the like.
Owner:HUNAN AGRICULTURAL UNIV
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