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Industrial preparation method of theaflavin

A technology of theaflavins and theaflavins esters, which is applied in the fields of organic chemistry and fermentation, can solve the problems of restricting the large-scale production of theaflavins, complex and expensive separation and purification procedures, etc. The effect of increased oxygen capacity

Inactive Publication Date: 2010-04-07
ZHEJIANG PAINUO BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, it still involves preventing theaflavins from being continuously oxidized into theaflavins and complex and expensive separation and purification procedures for the product, which restrict the large-scale production of theaflavins

Method used

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  • Industrial preparation method of theaflavin

Examples

Experimental program
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Effect test

Embodiment 1

[0029] (1) Prepare 700kg of ethyl acetate solution rich in 80mg / ml tea polyphenols by the process of extracting tea polyphenols;

[0030] (2) 700kg of ethyl acetate solution rich in tea polyphenols and 800kg of pulverized fresh tea leaves are dropped into an oxidation fermenter, followed by adding 20L of ethyl acetate and 1.5L of citric acid-disodium hydrogen phosphate buffer, at room temperature Under the state, the air is passed through to stir and oxidize and ferment for 30-40 minutes to obtain the theaflavin ester phase solution;

[0031] (3) The theaflavin ester phase solution is first filtered to remove the tea dregs, then concentrated and washed with sodium bicarbonate solution to obtain an aqueous phase solution and an ester phase solution; the tea dregs are dried and stored for later use.

[0032] (4) Spray-dry after the aqueous phase solution of step (3) is concentrated, obtain the theaflavin dry powder of low specification;

[0033] (5) the ester phase solution of ...

Embodiment 2

[0038] (1) Prepare 800kg of ethyl acetate solution rich in 80mg / ml tea polyphenols by the process of extracting tea polyphenols;

[0039] (2) 800kg of ethyl acetate solution rich in tea polyphenols and 800kg of pulverized fresh tea leaves are dropped into an oxidation fermenter, and 24L of ethyl acetate and 1.8L of citric acid-disodium hydrogen phosphate buffer are added successively. Under the state, the air is passed through to stir and oxidize and ferment for 30-40 minutes to obtain the theaflavin ester phase solution;

[0040] (3) The theaflavin ester phase solution is firstly filtered to remove tea dregs, then concentrated and washed with citric acid solution to obtain an aqueous phase solution and an ester phase solution; the tea dregs are dried and stored for later use.

[0041] (4) Spray-dry after the aqueous phase solution of step (3) is concentrated, obtain the theaflavin dry powder of low specification;

[0042] (5) the ester phase solution of step (3) is concentra...

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Abstract

The invention relates to an industrial preparation method of theaflavin, which comprises the following steps: (1) preparing an ethyl acetate solution containing rich tea polyphenol; (2) respectively adding pulverized fresh tea leaves and the ethyl acetate solution into a oxidation-fermentation tank, orderly adding ethyl acetate and a buffering solution, introducing air at normal temperature, and stirring, oxidizing and fermenting to obtain the theaflavin ester-phase solution; (3) filtering the theaflavin ester-phase solution, concentrating and washing to remove impurities; (4) concentrating the water-phase solution, and spray-drying to obtain low-specification dry theaflavin powder; (5) concentrating the ester-phase solution to recycle ethyl acetate, and adding water so that the theaflavin is dissolved in the water; (6) centrifugally filtering the theaflavin water solution to obtain a pure theaflavin solution; (7) and concentrating the theaflavin solution, and spray-drying to obtain the dry theaflavin powder. The method of the invention simultaneously enhances the yield and the quality of the theaflavin, saves the energy and the man hours and reduces the emission of waste water.

Description

(1) Technical field [0001] The invention relates to an industrialized method for preparing theaflavins from tea leaves. (2) Background technology [0002] In recent years, as modern medical research has discovered that theaflavins in black tea have strong biological activity, their superiority in function and their application in natural medicine, functional food and beverage, and daily chemical industry have become more and more broad. Extracting theaflavins from black tea is not economically cost-effective, so different methods for preparing theaflavins have been reported one by one. Among them, the preparation of theaflavins by in vitro oxidation (enzymatic oxidation or chemical oxidation) of tea polyphenols is a future trend. Although finding new enzyme sources and their immobilization technology and new oxidants is an important task for the preparation of theaflavins from tea polyphenols in vitro simulated oxidation, but this is an aerobic process, so "two-liquid phase...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P17/16C07D311/62
Inventor 金克云谢金虎杨贤强王岳飞
Owner ZHEJIANG PAINUO BIO TECH
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