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Application of theaflavin as feed additive and corresponding feed

A feed additive, theaflavin technology, applied in the direction of animal feed, animal feed, application, etc., can solve the problems of feed rancidity, reduced palatability, toxic and side effects, etc., to promote weight gain, avoid drug residues, and avoid toxic and side effects. Effect

Active Publication Date: 2012-07-04
SOUTH CHINA AGRI UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0013] Secondly, some nutrients in aquatic feed are easily affected by temperature and humidity during processing and storage, and are oxidized to produce aldehydes and acids, which make the feed rancid and deteriorate and reduce the palatability.
In order to reduce the loss of nutrients, improve feed utilization efficiency, and prolong the shelf life, it is necessary to add antioxidants. However, the currently used ethoxyquin, dibutyl hydroxytoluene, and butyl hydroxyanisole all have more or less toxic and side effects. has been banned from using

Method used

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  • Application of theaflavin as feed additive and corresponding feed
  • Application of theaflavin as feed additive and corresponding feed
  • Application of theaflavin as feed additive and corresponding feed

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Embodiment 1: Feed preparation method using theaflavin as a feed additive.

[0034] The raw materials of the formula shown in Table 1 are crushed through 60 meshes, and trace ingredients such as theaflavin and choline chloride are added step by step, mixed with large raw materials evenly, and made by SLX-80 extrusion machine Feeds with a diameter of 1.2mm were dried and cooled at 45°C and put into sealed bags and stored in a -15°C refrigerator until use. And the formula is prepared as the control feed shown in Table 1 in the same way.

[0035] Table 1 Test feed formula and nutrient composition (dry matter, %)

[0036]

[0037]

[0038] In the above feed formula, fishmeal and soybean meal are the main protein sources, fish oil and soybean oil are the main fat sources, and high-gluten flour is the main sugar source.

[0039] Among them, 1000g vitamin premix includes: vitamin A, 250000IU; vitamin D, 120000IU; vitamin K3, 500mg; vitamin E, 3600mg; vitamin B1, 550mg;...

Embodiment 2

[0041] Example 2: Effects of theaflavins on weight gain rate and specific growth rate of Litopenaeus vannamei

[0042] 1. Test method

[0043] 1.1 Feeding management

[0044] The breeding experiment was carried out in the indoor aquarium breeding system of Nan'ao Linhai Experimental Station of Shantou University. The experimental shrimps were purchased from a fine-bred shrimp nursery in Shantou City. They were first domesticated in the aquarium for 2 weeks, and then the healthy and healthy Litopenaeus vannamei with an initial average weight of 0.1±0.05g / tail were randomly divided into boxes and carried out. Breeding test.

[0045] One group is the control group, and the three groups are the test groups. Each group has three parallels, and each parallel has 50 tails. The control group was fed with basic feed, and the test group was fed with feed containing 0.015%, 0.03% and 0.045% theaflavin respectively. The breeding test was carried out for two months. Except for changing...

Embodiment 3

[0061] Example 3: Effects of theaflavins on main components of whole shrimp and muscle of Litopenaeus vannamei

[0062] 1. Test method

[0063] 1.1 feeding management: same as embodiment 2

[0064] 1.2 Test method

[0065] Accurately measure the feeding amount every day, collect hepatopancreas and muscle samples every 20 days, and take three samples in total. Blood is collected once after the test is over. crude fat. Stop feeding for 24 hours before each sampling, randomly select about 10 shrimps for each repetition, and use a sterile 1mL syringe to insert the blood from the shrimp head behind the carapace to collect blood, put it in an Eppenddorf centrifuge tube, and let it stand overnight at 4°C. Then centrifuge at 3000r / min for 10min, take the supernatant as the serum to be tested, and store it frozen for later use. After taking out the hepatopancreas and muscle, put it into an Eppenddorf centrifuge tube and store it in a -80°C refrigerator.

[0066] Homogenate preparat...

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PUM

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Abstract

The invention provides application of theaflavin as a feed additive and corresponding feed, belonging to the technical field of feed additives. The first purpose of the invention is to provide an application of the theaflavin as a feed additive, and the use method is that the weight of the theaflavin is added according to the total weight of the feed, namely, being 0.015-0.045% of the total weight. The second purpose of the invention is to provides a feed using the theaflavin as the feed additive. The feed comprises the components of fish meal, bean pulp, peanut meal, cuttlefish cream, beer yeast, shrimp shell powder, fish oil, soya-bean oil, choline chloride, soyabean lecithin, monocalcium phosphate, vitamin premix, high gluten flour and theaflavin. According to invention, the theaflavin is taken as the feed additive, so that the immunocompetence of bred animals is improved, and the problems of drug residue and the like caused by using antibiotics are avoided; and the oxidation resistance of the bred animals can be improved, so that the toxic and side effects caused by adding chemosynthesis antioxidant are avoided. Theaflavin can be widely used in feed additives.

Description

technical field [0001] The invention relates to a feed additive, in particular to a natural product feed additive. Background technique [0002] Since Roberts E.A.H. (1957) discovered theaflavin (theflavin), theaflavin has been a hot spot in tea research. Theaflavin is a general term for a class of compounds formed by the oxidation of polyphenols that are soluble in ethyl acetate and have a benzotropolone structure. It is one of the important taste factors of black tea (Wan Xiaochun et al., 2001), with strong biological activity. Its structural formula is as follows: [0003] [0004] Modern medicine has found that tea pigment (the main components are theaflavin and thearubigin) not only has antibacterial, antiviral, anti-cardiovascular diseases, etc. All diseases have good preventive and therapeutic effects, among which theaflavins have the most significant medicinal effect. Known as the "soft gold" in tea, theaflavins account for less than 0.3% in finished black tea...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K1/16
Inventor 温小波史合群谢晓辉张云
Owner SOUTH CHINA AGRI UNIV
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