The invention belongs to the technical field of
food safety and
rapid detection, and particularly relates to a time-resolved fluorescent probe for rapidly detecting
ethoxyquin, an immunochromatography test strip, a detection method and application. The probe Eu-EQ-mAb is prepared by
indirect coupling of
europium fluorescent microspheres and
ethoxyquin monoclonal antibodies, the particle size of the microspheres is 150-250 nm, and the emission peak is 610-625 nm. The test strip comprises a PVC (
polyvinyl chloride) bottom plate, a sample pad, a
nitrocellulose membrane coated with a detection line T and a
quality control line C, and a water absorption pad, during detection, a sample and the probe are pre-reacted and then are subjected to
chromatography, and a result is judged through a T /
C value. During detection, after the Eu-EQ-mAb fluorescent probe and a sample are subjected to pre-reaction, immunochromatography is carried out, and a T /
C value judgment result is read. The semi-quantitative
detection limit of the method is 0.5-1 [mu] g / L, the detection sensitivity of the method in large yellow croakers, perches and pears reaches 5 [mu] g / kg, intra-batch and inter-batch variation coefficients are smaller than 10%, and the method has the advantages of being easy and convenient to operate, rapid in detection, high in sensitivity and the like and is suitable for on-site rapid screening of
ethoxyquin residues in food.