19 results about "Primary sites" patented technology

Systems, computer program products, and methods are described herein for auto-establishing data transfers between data centers for batch processing. The present disclosure is configured to identify a process associated with a primary site; determine a backup site associated with the primary site; apply the process to the backup site; generate, by an imitation artifact component, a record counter of the process as the process runs in the primary site, wherein the record counter comprises a cursor position indicating a current operation of the process; and mimic, based on the record counter, the process in the backup site as the process runs in the primary site.

The present application relates to the technical field of nucleic acid detection, in particular to a RNA pathogen chemiluminescence detection method based on nucleic acid structure and sequence characteristics. The present application provides a pathogen RNA rapid detection technology based on the reporter molecule cyclization combined with rolling circle amplification according to the secondary structure and sequence characteristics of RNA, which is suitable for high-sensitivity and high-specificity detection of RNA pathogens such as respiratory viruses. The present application innovatively combines the target structure characteristics with sequence specificity, breaks through the traditional sequence-dependent isothermal amplification technology, realizes the highly specific reporter molecule cyclization, significantly improves the detection reliability, and does not require complex instruments. After the swab sample is collected, the pathogen RNA is rapidly released by using a nucleic acid releasing agent, without complex purification steps, and the reading can be realized through a common chemiluminescence instrument, which is suitable for primary site detection.

The application provides a cancer primary focus tracing method, device, system and storage medium, and relates to the technical field of cancer tracing. The cancer primary focus tracing method comprises the following steps: acquiring sample tissue data, giving each cancer sample a primary focus label through a methylation chip; determining candidate CpG sites; and constructing a cancer tracing classifier. The application realizes the technology of tracing multiple cancers by using DNA methylation biological characteristics and machine learning methods, and has the following characteristics: due to the high stability of DNA molecules, cancer information is relatively completely retained, so that sample storage and transportation are more convenient; the cancers targeted by the application have a wide coverage range and include general common tumors; cancer-specific CpG sites have a wide coverage; through analysis of the cancer-specific sites, the interference of normal tissues is excluded, so that the accuracy and application range of the classifier are improved; and the high-precision classifier makes the cancer diagnosis process simpler, more accurate and more efficient.

A computer system includes a primary site having a primary volume, and having an instance that performs data processing related to input / output data, and a secondary site having a secondary volume, and having an instance. Remote copy is set up between the primary and secondary volumes, and the instance of the primary site transfers data input to or outputted from the primary volume to the secondary site, while the instance of the secondary site stores the transferred data in the secondary volume. The computer system further includes a specifications changing section that changes specifications of the instance of the secondary site when a failover switching process of switching a performer of the data processing from the primary to secondary site is performed, or when a failback switching process of switching the performer of the data processing from the secondary to primary site after the failover switching process is performed.

A method and system for data synchronization between a standby site and a primary site in a distributed database system is provided. A first primary site included in a primary site group of the distributed database system generates a commit action redo log message for a commit action performed by the first primary site on a first database transaction, the commit action redo log message including a transaction ID of the first database transaction and a transaction order indicator representing an order of database transactions in a group of database transactions executed on one or more primary sites in the primary site group. The first primary site sends the commit action redo log message to a corresponding first standby site backing up the first primary site.

This application relates to a data reading method, apparatus, electronic device, and storage medium. The method includes: receiving a data reading request, the data reading request including index information of a target object; obtaining the synchronization status of the target object; in response to an incomplete initial synchronization, determining the primary site as the target site and a first local storage bucket as the target data address; reading target object data from the first local storage bucket according to the index information; in response to a completed initial synchronization, obtaining the deletion status of the target object and the deletion policy of the object storage system; determining the target site and the corresponding target data address based on the deletion policy and deletion status; and reading the target object data from the target data address of the target site according to the index information. This method ensures that the data obtained by the user on different sites remains consistent and is always the latest version of the object.

Methods for predicting a cutaneous primary site of disease are described. The methods may comprise, for example, receiving, using one or more processors, sequence read data associated with a sample from the individual, selecting, using the one or more processors, a plurality of reads from the sequence read data, determining, using the one or more processors, an ultra-violet (UV) signature metric based on the selected plurality of reads, inputting, using the one or more processors, the UV signature metric into a statistical model, and predicting, using the one or more processors, the primary site of the disease in the individual based on an output of the statistical model.

Systems and methods for allowing and maintaining Input / Output (I / O) operations in a distributed storage system with multiple storage sites when communications links between the sites fail are provided. Mirrored storage sites use an update policy, including an update record and a bitmap configured to track changes in memory blocks storing an I / O request. When a link between two or more sites fails, one or more of the sites may be designated as a primary site, on which normal I / O operations may continue. One or more sites may also be designated as non-primary sites and are “detached” with respect to the primary site or sites. I / O operations on the non-primary sites may be suspended until the link is restored. When the link is restored, the update records and bitmaps are used to rebuild the detached sites.

Method, computer program product, and computer system are provided. A controller component verifies the state of all file sets. The controller component initiates a sub-controller component, based on successfully verifying the state of the file sets. The sub-controller component suspends write operations to all databases at the primary site computer. Based on the suspending completing without error, the sub-controller executes point-in-time copy processes for each of the databases and for application non-database files at the primary site computer. Applications using the databases and non-database files are not suspended. Based on all of the point-in-time copy processes completing without error, the sub-controller component resumes write operations to all databases at the primary site computer. The point-in-time copies are automatically sent to the secondary site computer so that the applications can operate at the secondary site computer if the primary site computer becomes unavailable.

A computer-implemented method for generic tenant data replication for disaster recovery, includes regularly checking, by a primary replication agent on a primary site, for changes to a primary change log on the primary site. Based on an insert of a new change record in a secondary change log on a secondary site, triggering a stored procedure from secondary stored procedures on the secondary site, that extracts a change operation and data from the secondary change log and performs the changed operation on a secondary tenant table on the secondary site. By a secondary replication agent on the secondary site and from the primary replication agent, receiving a call not containing a sequence id for a change record marked as completed in the secondary change log, and deleting the change record. Using a replication agents control plane, providing central monitoring for the primary replication agent and the secondary replication agent.

Techniques for migration or disaster recovery of vTPM enabled virtual machines include non-transitory computer-readable media storing program instructions that, when executed by one or more processors associated with a computing device, cause the one or more processors to perform a method including transmitting, by a primary site, an encryption secret for an encrypted storage device to a secondary site, the encrypted storage device storing data encrypted based on the encryption secret; and transmitting, by the primary site using an unsecure channel, the data as encrypted based on the encryption secret to the secondary site.

The invention discloses a UvsY auxiliary protein mutant with improved thermal stability, the thermal stability of the UvsY auxiliary protein mutant is remarkably improved, and the core technical problem that an existing UvsY auxiliary protein is poor in thermal stability is solved. In addition, the UvsY auxiliary protein mutant can still maintain efficient in-vitro nucleic acid amplification activity after being stored for a long time, the peak appearance time is short, the amplification efficiency is stable, strict cold chain transportation and storage are not needed, and the application cost is reduced. The method can be widely applied to various scenes such as RPA and RTMA isothermal amplification technologies, adaptive gene detection and basic-level field diagnosis, and has extremely high practical application value and industrialization prospect.

The invention belongs to the technical field of molecular detection of biological medicines, and particularly relates to a nucleic acid extraction-free SFTSV detection kit, primer probes and a method. The primer probe composition comprises an upstream primer SEQ ID NO: 1 for specifically recognizing the SFTSV, a downstream primer SEQ ID NO: 2 of which the 5'end is labeled with biotin, and a probe SEQ ID NO: 3 of which the 5 'end is labeled with a reporter group and contains THF modification. The kit comprises the composition, a nucleic acid releasing agent and a lateral flow chromatography test strip. The method comprises the following steps: treating a serum / plasma sample by using a nucleic acid releasing agent, carrying out RPA isothermal amplification by directly taking a lysate as a template, and detecting a result through a test strip. The RPA-LFD rapid detection of the SFTSV is realized, the sensitivity (200copies / mL) and the specificity are high, the operation is simple, convenient and rapid (about 40 minutes), a complex instrument and a nucleic acid extraction step are not needed, and the method is suitable for base-level field screening.

The invention discloses an enzyme signal amplified mycobacterium rapid detection kit and a detection method, and relates to the technical field of in vitro diagnosis. Aiming at the current situations that a culture method is time-consuming, PCR (Polymerase Chain Reaction) equipment is expensive and a rapid detection strip is low in sensitivity, the invention provides an enzyme signal amplified mycobacterium rapid detection kit and a detection method, an upper cover and a lower cover of a shell are buckled to form a cavity, a chromatography strip assembly is attached to a PVC (Polyvinyl Chloride) back plate in the cavity, the assembly is formed by sequentially overlapping a sample adding pad, a combining pad, an NC (Nitrocellulose) membrane and an absorbing pad, and a T line and a C line are arranged on the NC membrane; an HRP labeled antibody is pre-embedded in the conjugate pad, and after detection, a chromogenic substrate is dropwise added to trigger multiple rounds of enzymatic reaction amplification signals. The structure is matched with an annular sealing ring and a drying agent, interpretation can be performed by naked eyes or a reading instrument within 15 min, the lowest detection limit reaches 10-12 CFUmL, the false negative risk of low bacterial quantity is reduced, and the rapid screening requirement of a basic level site is met.

The invention relates to the technical field of biological information, in particular to a generic cancer brain metastasis molecular typing system and method and a medium. According to the generic cancer brain metastasis molecular typing system, brain metastasis molecular typing obtained through a data processing and consensus clustering method is kept stable in multiple queues and multiple platforms, and reproducibility and robustness are achieved. The typing system disclosed by the invention can identify four subtypes with consistent biological connotation in general cancer brain metastasis, can realize unified brain metastasis typing across primary parts, and avoids the limitation of typing only depending on primary lesion. The four subtypes correspond to different states such as neuron-like, immune infiltration, metabolic reprogramming and proliferation procedures and can be consistently verified on the proteome / pathway level, and biological interpretation is high. The four types can correspond to prognosis differences, immune / metabolic landscapes and other characteristics, and a basis is provided for risk stratification and potential treatment strategy selection.

Systems and methods for allowing and maintaining Input / Output (I / O) operations in a distributed storage system with multiple storage sites when communications links between the sites fail are provided. Mirrored storage sites use an update policy, including an update record and a bitmap configured to track changes in memory blocks storing an I / O request. When a link between two or more sites fails, one or more of the sites may be designated as a primary site, on which normal I / O operations may continue. One or more sites may also be designated as non-primary sites and are “detached” with respect to the primary site or sites. I / O operations on the non-primary sites may be suspended until the link is restored. When the link is restored, the update records and bitmaps are used to rebuild the detached sites.

An embodiment provisions a Validation Area within a consistency group of a primary site and a Validation Area within a consistency group of a secondary site. The embodiment provisions a Validation Writer in the primary site and provisions a Validation Reader in the secondary site; the Validation Writer writes a validation metric into the Validation Area in the primary site at an interval determined by a recovery point objective. The embodiment also reads the validation metric by the Validation Reader in the secondary site, computes a validation result based on the validation metric, the recovery point objective and a recovery point time delta where the validation result comprises an outcome of a replication of the primary site to the secondary site where the Validation Area of the primary site, the Validation Area of the secondary site, the Validation Writer and the Validation Reader are storage and application agnostic.

This application provides a site switching method, apparatus, and device for a centralized flash dual-active system. The method, applied to an arbitration server, includes: monitoring the status of the heartbeat link and / or data synchronization link between two sites in the dual-active system; when the heartbeat link and / or data synchronization link are interrupted, designating both sites as candidate sites; generating a data reporting instruction and sending it to each candidate site; receiving logical consistency identifiers and corresponding network connectivity probe results from each candidate site; and authorizing a candidate site as the new primary site when the logical consistency identifier of one and only one candidate site is greater than that of the other candidate sites, and the network connectivity probes between that candidate site and all predetermined service hosts are successful. The method provided in this application ensures that the new primary site after switching can actually carry out services, avoiding service interruption after switching to a new primary site.

The invention belongs to the technical field of bioinformatics, and discloses a method and device for predicting osteosarcoma survival prognosis and electronic equipment, which are used for predicting osteosarcoma survival prognosis by incorporating more cases and detailed clinical baseline data and combining clinical prognosis factors such as primary focus part factor values, tumor staging factor values, tumor diameter factor values and serum alkaline phosphatase factor values. And one or more of the screened super enhancer driving genes LACTB, CEP55, SRSF3, TCF7L2 and FOXP1 with better prediction efficiency are used as gene combinations to jointly construct a prognosis prediction model for survival risk assessment, so that prognosis prediction of a patient corresponding to a to-be-detected sample is realized, the prediction model is more reliable in efficiency, and the prediction efficiency is higher. The prediction accuracy of osteosarcoma survival prognosis can be obviously improved.