49 results about "Hepatopancreas" patented technology

The invention discloses a scylla paramamosain keep-alive and soft shell soft-keeping method, a low-temperature keep-alive mode refers to an on-ice keep-alive mode or a low-temperature acidification keep-alive mode, preferably, the low-temperature acidification keep-alive mode: cooling seawater to 16 DEG C or below by using a cooling-water machine, then adjusting the pH value of the seawater to be less than 8 to obtain low-temperature acidified seawater, and then cooling the low-temperature acidified seawater to obtain the scylla paramamosain soft shell soft-keeping method. And putting the soft-shell crabs which just molt into low-temperature acidified seawater for keeping alive and softness. The hardening of the soft shell can be obviously delayed by low-temperature acidified seawater and on-ice keep-alive; low-temperature acidified seawater and on-ice keep-alive soft-shell crabs can improve the delicate flavor and sweet taste of hepatopancreas of the soft-shell crabs, and the flavors of the two keep-alive soft-shell crabs are better than those of cold-stored dead soft-shell crabs; on-ice and low-temperature acidified seawater keep-alive interferes with glycolipid metabolism, oxidative stress and cell apoptosis of the soft-shell crabs, and compared with on-ice keep-alive, low-temperature acidified seawater keep-alive influences are milder than on-ice keep-alive.

The invention discloses a lactobacillus plantarum MW 2406C, which is preserved in China General Microbiological Culture Collection Center (CGMCC) on January 9, 2025, and the preservation number of the lactobacillus plantarum MW 2406C is CGMCC No. 33339. The inventor finds that the bacterial strain has better oxidation resistance, pathogenic bacterium inhibition and oxygen resistance, and can be used for effectively fermenting feed, pre-decomposing nutritional ingredients in the feed and improving the utilization efficiency of the feed; in addition, the strain can improve liver and intestine health and improve immunity by enhancing hepatopancreas antioxidant enzyme activity and intestinal digestive enzyme activity, improving intestinal barrier health, up-regulating antibacterial peptide level and reducing inflammatory factors. When the lactobacillus plantarum is used for preparing the fermented feed for the river crabs, the utilization efficiency of the compound feed by the river crabs is improved, the intestinal health of the river crabs is improved, and the disease resistance is improved.

The invention discloses an anti-lipopolysaccharide factor rALF-like protein and application thereof, and belongs to the technical field of molecular biology. The anti-lipopolysaccharide factor rALF-like protein provided by the invention belongs to secretory protein, the rALF-like protein can be competitively combined with an LPS structural domain of bacteria to achieve the purpose of inhibiting inflammation, and the expression of the coding gene Pv-ALF-like gene in blood cells, hepatopancreas, gill and intestinal tissues is remarkably up-regulated, so that the anti-lipopolysaccharide factor rALF-like protein can be used for inhibiting inflammation. The rALF-like protein disclosed by the invention has antibacterial and antiviral effects, lays a foundation for developing antibacterial and antiviral drugs and functional feed additives, and plays an important role in improving the resistance link of prawns.

The present application belongs to the field of aquaculture technology, and mainly relates to a traditional Chinese medicine composition for preventing and treating acute hepatopancreas necrosis disease of prawn, a preparation method and application. The traditional Chinese medicine composition comprises the following raw materials in parts by weight: 28-32 parts of Radix Sanguisorbae, 28-32 parts of Radix Scutellariae, 18-22 parts of Phellodendri Cortex and 8-12 parts of Lonicerae Japonicae Flos. The traditional Chinese medicine composition can significantly inhibit the activity of the main pathogen of the disease, i.e. Vibrio parahaemolyticus, improve the immune function and disease resistance of prawn, and further repair the hepatopancreas and intestinal tissues of the diseased prawn, thereby achieving the effect of effectively preventing and treating the acute hepatopancreas necrosis disease of prawn.

The invention discloses a multiplex fluorescent quantitative PCR (polymerase chain reaction) detection kit for prawn culture pathogenic bacteria, which is characterized in that the kit contains primers and probes for detecting the prawn culture pathogenic bacteria, wherein the primers and the probes are respectively used for detecting TDH-related hemolsin genes of vibrio parahaemolyticus, ORF107 genes of white spot syndrome viruses, small subunit ribosomal genes of shrimp enterocytozoon hepatopenaei, 37 kDa coat protein genes of infectious subcutaneous and hematopoietic necrosis viruses, DNA (deoxyribonucleic acid) primer genes of full-eye iridovirus 1 and PirA [pi] genes of pathogens of bacterial prawns acute hepatopancreas necrosis; the method has the advantage that the detection of eight prawn culture pathogens can be realized at the same time. The method comprises the following steps of: obtaining a tcdB gene of a high-pathogenicity vibrio and a hemolsin gene of a vibrio harveyi;

The invention provides heat-resistant bacillus pumilus P2-7 and application thereof in disease prevention and control of prawn culture, and belongs to the technical field of bioengineering. The heat-resistant bacillus pumilus P2-7 provided by the invention is preserved in Guangdong Microbial Culture Collection Center on November 4, 2024, and the preservation number of the heat-resistant bacillus pumilus P2-7 is GDMCC NO. 65408. The strain is different from the traditional antibacterial bacillus in antagonism mode, cannot secrete metabolites with antibacterial activity, plays a role in a phagocytosis mode, can remarkably remove / inhibit pathogenic bacteria in water, can effectively prevent and control shewanella disease of prawns, and has a good application prospect. The composition also has a good effect of simultaneously preventing septicemia, acute hepatopancreas necrosis, white spot syndrome and infectious subcutaneous and hematopoietic necrosis in prawn culture production.

The invention discloses a bacillus safensis strain and application thereof in aquaculture, and relates to the technical field of aquaculture disease prevention and control, the obtained bacillus safensis strain ZY-1 forms spores under a certain fermentation condition, can be prepared into powder for preservation, has a long shelf life, has the bacteriostatic activity retention rate of more than or equal to 85% after being stored in a dark place for 12 months at 4-25 DEG C, and has the antibacterial activity of more than or equal to 7.2%. The production, transportation and storage cost is saved, main bacteriostatic effect factors of the bacillus safensis ZY-1 strain exist in extracellular metabolites, the bacillus safensis ZY-1 strain is stable in performance and resistant to high temperature, acid and alkali, ultraviolet rays, protease and the like, and fermentation supernatant of the bacillus safensis ZY-1 strain can be directly applied or prepared into spray-dried powder and is suitable for various aquaculture application scenes such as pond splashing and feed mixing and feeding. The metabolite spray-dried powder has a remarkable killing effect on high-pathogenicity vibrios VpAHPND and VpTPD, the minimum inhibitory concentrations of metabolite spray-dried powder respectively reach 23.44-46.88 mu g / ml and 46.88-93.75 mu g / ml, and the metabolite spray-dried powder can be used for preventing and controlling prawn infectious hepatopancreas necrosis and prawn transparent larva diseases induced by the metabolite spray-dried powder and also has a certain killing function on pathogens of other aquatic animals.

The application relates to a feed additive of herbal extracts for Litopenaeus vannamei and application thereof. The feed additive of herbal extracts is composed of dandelion extracts and houttuynia cordata extracts. The dandelion extracts and the houttuynia cordata extracts are mixed in a proportion of 1:1 and then added into the basic feed of Litopenaeus vannamei, so that the growth performance of the Litopenaeus vannamei is remarkably improved, the intestinal and hepatopancreas tissue structures are improved, and the immunity is enhanced. Meanwhile, the extracts effectively regulate the intestinal flora structure, increase the abundance and diversity of the intestinal flora, and promote the nutrient absorption and digestion functions. The extracts have a synergistic effect, are low in price, green in environmental protection, safe and free of residues.

The invention provides a breeding method suitable for breeding litopenaeus vannamei in saline-alkali soil, and relates to the technical field of aquaculture. According to the breeding method suitable for breeding the litopenaeus vannamei in the saline-alkali soil, the proportion of Ca < 2 + > to Mg < 2 + > to K < + > in a water body is optimized to be 2.8: 1: 0.3 by customizing a compound ion regulation and control solution, SO4 < 2-> / Cl <-> is controlled to be 0.14-0.8, and the pH is stabilized to be 8.2-8.8 in combination with a citric acid-sodium citrate buffer system; a slow-medium-fast segmented domestication strategy is adopted to adapt to different development stages of the fries; ethanol clostridium protein (CAP) is added into the feed to be combined with compound probiotics, so that immune metabolism regulation and control are enhanced. Experiments prove that according to the method, the survival rate of the litopenaeus vannamei seedlings reaches 86.3% + / -2.1%, the culture period is shortened by 12-15 days, the hepatopancreas SOD activity is improved by 42.6%, the vibrio inhibition rate reaches 78.5%, and the method is remarkably superior to the prior art. The method does not need large-scale pond transformation, adapts to different types of saline-alkali soil water bodies, realizes efficient green culture, and has important application value.

The invention relates to the technical field of aquaculture, in particular to ambrosia trifida dry powder for aquatic feed, a preparation method of the ambrosia trifida dry powder and elopichthys bambusa feed. The method comprises the following steps: feeding Aristichthys nobilis with Aristichthys nobilis leaves, then carrying out fasting and dissection, taking midgut and posterior gut wrapped by hepatopancreas of Aristichthys nobilis, and carrying out liposuction, sterilization and first drying; feeding ambrosia trifida leaves to the ampullaria gigas, and crushing the ambrosia trifida leaves to obtain minced ambrosia trifida; fermenting, standing and freeze-drying a mixture of the minced ambrosia trifida snail, methanol and corn protein powder to obtain ambrosia trifida fermented snail source bacterial powder; the method comprises the following steps: inoculating to-be-fermented ambrosia trifida powder and an ambrosia trifida zymophyte liquid into an ambrosia trifida fermentation liquid culture medium, and carrying out fermentation culture, first centrifugation and second drying. By means of the method, the content of toxic substances in ambrosia trifida can be reduced, the toxic substances are converted into nutrient substances capable of being used for growth of elopichthys bambusa, absorption and digestion of elopichthys bambusa are facilitated, and the cost of artificial breeding of elopichthys bambusa is reduced.

This invention discloses a method for detecting mitochondrial membrane potential in the gills or hepatopancreas of crustaceans, belonging to the field of mitochondrial membrane potential detection technology. This invention, by addressing the structural and physiological differences between gill and hepatopancreas tissues in crustaceans, formulates differentiated incubation strategies: for gill tissue, a "live pre-equilibration-short-time staining-rapid slide preparation" method is used, strictly controlling the JC-1 incubation time to within 10 minutes to effectively avoid probe toxicity damage to mitochondria; for hepatopancreas tissue, in vivo incubation is extended to 1 hour to ensure sufficient dye penetration. Under the condition of uniformly using 2 μM JC-1 and 20 μmol / L CCCP as positive controls, this method significantly improves the tissue penetration and signal uniformity of the dye, solving problems such as uneven staining and weak signal caused by exoskeleton barriers and high lipid content, providing reliable technical support for aquatic health assessment and toxicology research.

The invention belongs to the technical field of cell culture, and particularly relates to a litopenaeus vannamei hepatopancreas blister cell separation and in-vitro culture method which comprises the following steps: S1, obtaining hepatopancreas of litopenaeus vannamei; s2, mixing the hepatopancreas in the step S1 with trypsin, and performing sieving treatment to obtain a sieved solution; s3, performing precipitation resuspension and Percoll treatment on the sieved liquid obtained in the step S2 for multiple times to obtain a cell suspension; and S4, inoculating the cell suspension in the step S3 into a culture medium, sealing the culture medium, and placing the culture medium in an incubator. The hepatopancreas B cells of the litopenaeus vannamei obtained through separation and purification by a density gradient centrifugation optimization method are complete in morphology and relatively good in activity. Successful separation of the B cells is beneficial to research on change and immune response of the B cells in pathogen infection, and a technical support is provided for follow-up research on a molecular basis of immune function differences of different types of hepatocytes.

The invention provides a river crab hepatopancreatic necrosis syndrome model, a construction method and application, and belongs to the technical field of aquaculture. According to the invention, aflatoxin B1 (AFB1) is utilized to construct a river crab hepatopancreatic necrosis syndrome model, and river crabs treated by the AFB1 have pathological characteristics such as severe hepatopancreatic atrophy, erosion, necrosis, color lightening, hepatopancreatic index reduction, body cavity effusion and the like, and are highly matched with typical symptoms of the river crab hepatopancreatic necrosis syndrome (commonly known as' Shrimp disease '). According to the river crab hepatopancreatic necrosis syndrome model, the blank of risk assessment of the river crab hepatopancreatic necrosis syndrome is filled, and theoretical support can be provided for multi-etiological mechanism analysis and early diagnosis marker screening of the river crab hepatopancreatic necrosis syndrome; a standard reference model is provided for development of drugs for preventing or treating the hepatopancreatic necrosis syndrome of the river crabs, and the method has important application value for promoting healthy culture of aquatic products.

The invention relates to a bacillus subtilis, and in particular to a screened bacillus subtilis JK-2 having an antagonistic effect on pathogenic bacteria of aquatic animals; the bacillus subtilis JK-2 of the invention can be used to antagonize pathogenic bacteria of aquatic animals; the bacillus subtilis JK-2 of the invention can significantly improve the activity of lysozyme in the serum and hepatopancreas of Chinese mitten crabs, can enhance the ability of Chinese mitten crabs to eliminate pathogenic bacteria, and can be used for the prevention and treatment of diseases of Chinese mitten crabs.

The invention discloses application of oleic acid in improving disease resistance and meat quality of prawns. Experiments prove that by adding 1% of oleic acid into the feed, defensive lipid droplets of the prawns can be activated, the in-vitro antibacterial activity of lipid droplet protein can be enhanced, the load of vibrio parahaemolyticus in hepatopancreas and hemolymph can be reduced, and hepatopancreas and intestinal tissue damage caused by infection can be relieved, so that the disease resistance of the prawns can be remarkably improved. Meanwhile, 1% of oleic acid is added, so that the muscle texture of the prawns can be improved, the adhesiveness, the chewiness and the hardness are improved, muscle fibers are arranged more tightly, the density is higher, and the eating quality of the prawns is effectively optimized. The invention provides a basis for the application of oleic acid as a green feed additive in the aspects of healthy culture and quality improvement of prawns, and has important production practice value.

The application belongs to the technical field of disease prevention and treatment of aquaculture, and particularly relates to application of honokiol combined with sulfisoxazole sodium in preparation of a Vibrio parahaemolyticus inhibitor, characterized by that the mass ratio of honokiol to sulfisoxazole sodium in the composition is 1:0.5-2, the concentration of honokiol in the composition is 32-64 µg / mL, and the concentration of sulfisoxazole sodium in the composition is 32-128 µg / mL, and the application further provides a medicine for improving sensitivity of Vibrio parahaemolyticus to sulfisoxazole sodium and treating acute hepatopancreas necrosis disease of prawns, and the mass ratio of honokiol to sulfisoxazole sodium in the medicine is 1:0.125-0.5; the composition can significantly reverse drug resistance of Vibrio parahaemolyticus to SMM-Na, reduce the use amount of SMM-Na, and produce a rapid sterilization effect through combined action, and meanwhile, the composition exhibits excellent protection efficacy in prawn bodies.

The invention discloses a steppe red oomycete strain, a microbial agent thereof and application of the steppe red oomycete strain in aquaculture, the steppe red oomycete strain is sent to China General Microbiological Culture Collection Center (CGMCC) for preservation, the preservation number is CGMCC No.35769, and the preservation date is August 28, 2025. The strain disclosed by the invention is subjected to anaerobic illumination culture in a conventional photosynthetic bacterium liquid culture medium to form a red viscous trait microbial agent, and the degradation rate of the microbial agent on H2S in water can reach 90% or above; meanwhile, the water purification effect is achieved when the fertilizer is applied to a culture pond with organic matter exceeding the standard, odor can be eliminated, and water can be cleared. The strain disclosed by the invention can effectively block toxic stress such as gill injury, hepatopancreas poisoning and the like of H2S to fish, shrimp and crab culture organisms and remarkably improve culture water environment and bottom mud quality, so that an efficient and environment-friendly novel microbial resource is provided for water H2S pollution treatment in aquaculture; the method is especially suitable for high-density pond culture and has wide practical application prospects and popularization value.

The present invention discloses a method for studying cholesterol replacement in Chinese mitten crab feed and promoting gonadal development. By measuring the growth performance of Chinese mitten crabs in different cholesterol content groups and different squalene content groups and the amount of cholesterol deposition in the hepatopancreas of juvenile Chinese mitten crabs fed with different diets, a linear model of the amount of cholesterol deposition in the hepatopancreas of juvenile Chinese mitten crabs fed with different cholesterol levels was prepared, and the biological efficacy of different amounts of squalene added to replace cholesterol was analyzed. This method can be used as an effective method for screening cholesterol substitutes. In addition, the test results show that, compared with the existing commercially available Chinese mitten crab feed and chilled fish farming, by adding 0.4% squalene to the basic formula of Chinese mitten crab feed, the poor growth and gonadal development of Chinese mitten crabs in the rearing period can be effectively improved, basically achieving the effect of chilled fish farming.

The invention belongs to the technical field of fish feed, and particularly relates to functional feed for promoting hepatopancreas of leuciscus waleckii to be healthy and application. The functional feed for promoting hepatopancreas health of the leuciscus waleckii comprises a basal feed and an additive chlorogenic acid, the addition amount of the chlorogenic acid is 0.02 to 0.08 percent. The functional feed capable of promoting hepatopancreas health of the leuciscus waleckii is simple in component, easy in raw material obtaining, safe and harmless, and by adding a proper amount of chlorogenic acid, the fish growth and feed utilization rate can be effectively improved, the lipid metabolism function, the immune function and the oxidation resistance are improved, and the growth performance and hepatopancreas lipolysis of the leuciscus waleckii are effectively promoted.

The invention discloses a river crab hepatopancreas index detection method and system based on hyperspectrum, and belongs to the field of aquatic product quality detection. Comprising the following steps: obtaining a river crab sample hyperspectral data set, and obtaining real morphological characteristics and actual hepatopancreas indexes of corresponding river crab samples; constructing a river crab form detection model, and identifying the area, length and width of the carapace of the river crab so as to obtain the real length and width of the river crab; constructing a river crab hepatopancreas index prediction network model which comprises a data screening module, a spectrum-space feature extraction module and a data fusion and prediction module; and predicting the hepatopancreas indexes of the river crabs by using the trained river crab form detection model and the trained river crab hepatopancreas index prediction network model. According to the method, the river crab hepatopancreas index prediction network model is constructed based on the hyperspectral data, physiological information of internal edible tissues of river crabs is obtained under the condition that the tissues of the river crabs are not damaged, and consumption of human resources and damage to river crab samples are reduced.

The application provides a use of a compound BMS309403 in preparation of a medicament for preventing and treating acute hepatopancreas necrosis disease of prawns, and belongs to the technical field of prawn disease prevention and treatment. It is unexpectedly found that an inhibitor of a fatty acid binding protein LvFABP of Litopenaeus vannamei, i.e. the compound BMS309403, can be effectively applied in the prevention and treatment of AHPND, which can not only reduce the disease of hepatopancreas and intestinal tract of prawns, but also effectively reduce the Vibrio virulence gene copy number of hepatopancreas of diseased prawns, so that the survival rate of prawns infected with acute hepatopancreas necrosis disease can be significantly improved. In addition, as a small molecule compound, the BMS309403 is more superior to small molecule double-stranded RNA in synthesis, preservation and use, and therefore can have a better prospect in the actual production application of AHPND prevention and treatment.

The present application belongs to the technical field of traditional Chinese medicine, and mainly relates to a traditional Chinese medicine composition for blocking bacterial vitreous body disease of shrimplets, a preparation method and application. The traditional Chinese medicine composition comprises the following raw materials in parts by weight: chebula 20-60 parts, schisandra 10-40 parts, and cyperus 10-40 parts. The traditional Chinese medicine composition can significantly reduce the mortality of shrimplets suffering from bacterial vitreous body disease (BVS), reduce the number of cultivable bacteria and the number of vibrio in the hepatopancreas of prawns, and has a good repair effect on the damage of the hepatopancreas and intestinal tract of the diseased shrimplets. The traditional Chinese medicine composition is simple and convenient to use, safe and green, can effectively reduce or replace the use of antibiotics, and is suitable for the control of vibrio diseases in the process of shrimplet cultivation and breeding production.

The invention discloses application of defatted insect protein sources in promoting ovarian development of female parent shrimps, ovarian development of the female parent shrimps can be promoted to different degrees after three defatted insect protein sources are added into feed, especially after defatted silkworm chrysalis meal is added, the defatted insect protein sources can be used as main protein sources, and the ovarian development of the female parent shrimps can be promoted to different degrees. The serum sex hormone level of female parent shrimps is remarkably improved, lysine degradation, amino acid biosynthesis, and enrichment of metabolites of metabolic pathways related to amino acids and fatty acids, such as glycerophospholipids, arachidonic acid and 2-oxo-carboxylic acid metabolic pathways, are increased, and the protein content of hepatopancreas and the accumulation of triglyceride of ovaries are effectively improved; the development of ovaries is promoted and the development quality of ovaries is improved.

The invention discloses a strain of spermaceti soxhlet ZX 2401Ca, which is preserved in China General Microbiological Culture Collection Center (CGMCC) on January 9, 2025, and the preservation number is CGMCC No.33340. The invention also discloses a method for preparing the spermaceti soxhlet ZX 2401Ca. The cetella sobolifera ZX 2401Ca is indigenous bacteria in the intestinal tract of the snakeheaded fish, and is obtained by screening from the intestinal tract of the snakeheaded fish for the first time. The strain can be metabolized to generate short-chain fatty acid mainly comprising acetic acid, and has very strong oxidation resistance. According to breeding experiments, the inventor finds that the fermented feed prepared from the strain has the functions of improving antioxidant capacity disorder, hepatopancreas injury and the like caused by oxidized fish oil, and also has a good effect in the aspect of improving the aeromonas hydrophila infection resistance of macrobrachium rosenbergii.

The present invention provides the application of Roseovarius litoreus in promoting the growth of Portunus trituberculatus. Another aspect of the present invention provides another use of Roseovarius litoreus, which is the application in the preparation of products for promoting the growth of Portunus trituberculatus. The present invention also provides a feed for Portunus trituberculatus, and viable bacteria of Roseovarius litoreus are added to the feed. The present invention discovers that Roseovarius litoreus can significantly promote the growth of Portunus trituberculatus, further improve the weight gain rate and hepatopancreas index of crabs, and has broad application prospects in aquaculture.

The invention provides a compound feed for juvenile penaeus vannamei as well as a preparation process and equipment of the compound feed. The compound feed comprises fish meal, euphausia superba powder, soybean meal, cottonseed protein, flour, soybean phospholipid oil, bile acid, carnitine, a vitamin premix, vitamin C phosphate, xanthophyll, methionine, choline chloride, monocalcium phosphate and a mineral premix. The compound feed is designed according to the physiological needs of juvenile penaeus vannamei in the liver conversion stage, and the health condition of hepatopancreas of the juvenile penaeus vannamei can be effectively improved by adding multivitamins, bile acid and carnitine, so that the juvenile penaeus vannamei is assisted to smoothly pass the liver conversion stage. Meanwhile, the feed intake can be remarkably increased, sufficient nutrition guarantee is provided for growth of the juvenile shrimps, and therefore the growth speed of the juvenile shrimps is increased. Besides, the preparation process comprises the steps of mixing for multiple times, crushing, conditioning, granulating, curing, cooling, sieving and the like, and special equipment is adopted for collaborative operation, so that the stable quality of the feed is guaranteed, and powerful support is provided for efficient culture of the penaeus vannamei boone.

The present invention discloses a detection device for detecting shrimp hepatopancreatic lesions based on images, which belongs to the field of shrimp farming technology, and comprises a box with a channel inside, an open inner cavity for shrimp to enter is provided at one end of the box, and the two ends of the channel are connected to the open inner cavity and the outside of the box respectively; lighting facilities are provided on the side walls of the open inner cavity for supplementing light to the open inner cavity and the channel; and the camera at the top of the channel can obtain pictures of the backs of the shrimps. Under the illumination of the lighting facilities, the shrimps swim towards the open inner cavity of the box towards the light, and the shrimps entering the channel obtain pictures of the backs of the shrimps through the top camera, and the camera uploads the images to a controller outside the breeding pond, and the controller analyzes and determines whether the shrimps' hepatopancreatic lesions occur according to the pictures of the shrimps' backs. The present invention can improve the accuracy of observation and judgment, timely observe the health of the shrimps' hepatopancreatic lesions during the breeding process, and take targeted and reasonable treatments according to the detected abnormalities of the hepatopancreatic lesions to ensure the healthy growth of the shrimps.

The invention discloses a prawn culture pathogenic bacteria multiple ERA detection primer and probe based on a biochip technology and application of the prawn culture pathogenic bacteria multiple ERA detection primer and probe. The kit is characterized in that the primers and the probe respectively aim at tcdB genes of highly pathogenic vibrios, hypothetical protein genes of white spot syndrome viruses, 16S rRNA genes of enterocytozoon hepatopenaei, non-structural protein genes of infectious subcutaneous and hematopoietic necrosis viruses, DNA primer enzyme genes of decapod iridovirus 1 and PirB genes of bacterial prawn acute hepatopancreatic necrosis pathogens; the method comprises the following steps of: detecting a capsid protein gene of a prawn infectious muscle necrosis virus, a capsid protein gene of a nedavirus, a capsid protein gene of the nedavirus, a structural protein ORF1 gene of the prawn infectious muscle necrosis virus, a non-structural polyprotein gene of a taura syndrome virus and a Rep-1b gene of a yellow head virus, and further discloses specific sequences. The sensitivity, the specificity and the accuracy are high.

The application provides a method for promoting synchronous maturation of parent shrimps, which comprises the following steps: temporarily breeding male parent shrimps and female parent shrimps in a pool; adjusting water flow velocity to perform water flow domestication and temperature domestication, setting the water flow velocity to be 0.4-1.5 m / s, and feeding nutrition enhancer to perform intensive feeding when the water flow domestication is performed; the nutrition enhancer comprises the following components: taurine, arachidonic acid, phosphatidylserine and cuttlefish bone powder; setting the temperature to be 18-19 DEG C, increasing the temperature by 0.4-0.6 DEG C per day, and increasing the temperature to 27-28 DEG C. The water flow domestication and the temperature domestication are performed by adjusting the water flow velocity, and the intensive feeding of the nutrition enhancer is combined when the water flow domestication is performed, which is beneficial to the accumulation of nutritional substances in the hepatopancreas tissue of the female shrimps, promotes synchronous development and maturation of the ovary, and can make the maturation rate of the female shrimps reach 100%, and the synchronous maturation rate reach 80%.

The invention relates to the technical field of aquatic feed, and discloses an oyster shell feed additive and an application of the oyster shell feed additive in shellfish breeding, and the additive comprises the following raw materials in parts by weight: 80-90 parts of oyster shell powder; 1-4 parts of a vitamin composition; 0.5 to 0.9 part of iron glycinate; 0.5 to 1.5 parts of zinc lysine; 0.2 to 0.9 part of manganese methionine; 0.22 to 0.45 part of copper lysine; the preparation method comprises the following steps: cleaning oyster shells, performing staged high-temperature calcination and crushing to obtain oyster shell powder, mixing the oyster shell powder with other raw materials under set conditions, and drying to obtain the oyster shell feed additive, the addition amount of the oyster shell feed additive in a crustacean animal feed is 1-5%, the oyster shell feed additive is fed 2-4 times every day, and the oyster shell feed additive is continuously fed until the culture is finished. By adding the oyster shell feed additive into the feed, the activity of immune enzyme in hepatopancreas is enhanced, the survival rate of crustaceans is effectively improved, and the mineral content of muscles and shells of the crustaceans is increased.