11 results about "Hepatopancreas" patented technology

The present application belongs to the field of aquaculture technology, and mainly relates to a traditional Chinese medicine composition for preventing and treating acute hepatopancreas necrosis disease of prawn, a preparation method and application. The traditional Chinese medicine composition comprises the following raw materials in parts by weight: 28-32 parts of Radix Sanguisorbae, 28-32 parts of Radix Scutellariae, 18-22 parts of Phellodendri Cortex and 8-12 parts of Lonicerae Japonicae Flos. The traditional Chinese medicine composition can significantly inhibit the activity of the main pathogen of the disease, i.e. Vibrio parahaemolyticus, improve the immune function and disease resistance of prawn, and further repair the hepatopancreas and intestinal tissues of the diseased prawn, thereby achieving the effect of effectively preventing and treating the acute hepatopancreas necrosis disease of prawn.

The invention provides a breeding method suitable for breeding litopenaeus vannamei in saline-alkali soil, and relates to the technical field of aquaculture. According to the breeding method suitable for breeding the litopenaeus vannamei in the saline-alkali soil, the proportion of Ca < 2 + > to Mg < 2 + > to K < + > in a water body is optimized to be 2.8: 1: 0.3 by customizing a compound ion regulation and control solution, SO4 < 2-> / Cl <-> is controlled to be 0.14-0.8, and the pH is stabilized to be 8.2-8.8 in combination with a citric acid-sodium citrate buffer system; a slow-medium-fast segmented domestication strategy is adopted to adapt to different development stages of the fries; ethanol clostridium protein (CAP) is added into the feed to be combined with compound probiotics, so that immune metabolism regulation and control are enhanced. Experiments prove that according to the method, the survival rate of the litopenaeus vannamei seedlings reaches 86.3% + / -2.1%, the culture period is shortened by 12-15 days, the hepatopancreas SOD activity is improved by 42.6%, the vibrio inhibition rate reaches 78.5%, and the method is remarkably superior to the prior art. The method does not need large-scale pond transformation, adapts to different types of saline-alkali soil water bodies, realizes efficient green culture, and has important application value.

This invention discloses a method for detecting mitochondrial membrane potential in the gills or hepatopancreas of crustaceans, belonging to the field of mitochondrial membrane potential detection technology. This invention, by addressing the structural and physiological differences between gill and hepatopancreas tissues in crustaceans, formulates differentiated incubation strategies: for gill tissue, a "live pre-equilibration-short-time staining-rapid slide preparation" method is used, strictly controlling the JC-1 incubation time to within 10 minutes to effectively avoid probe toxicity damage to mitochondria; for hepatopancreas tissue, in vivo incubation is extended to 1 hour to ensure sufficient dye penetration. Under the condition of uniformly using 2 μM JC-1 and 20 μmol / L CCCP as positive controls, this method significantly improves the tissue penetration and signal uniformity of the dye, solving problems such as uneven staining and weak signal caused by exoskeleton barriers and high lipid content, providing reliable technical support for aquatic health assessment and toxicology research.

The application belongs to the technical field of disease prevention and treatment of aquaculture, and particularly relates to application of honokiol combined with sulfisoxazole sodium in preparation of a Vibrio parahaemolyticus inhibitor, characterized by that the mass ratio of honokiol to sulfisoxazole sodium in the composition is 1:0.5-2, the concentration of honokiol in the composition is 32-64 µg / mL, and the concentration of sulfisoxazole sodium in the composition is 32-128 µg / mL, and the application further provides a medicine for improving sensitivity of Vibrio parahaemolyticus to sulfisoxazole sodium and treating acute hepatopancreas necrosis disease of prawns, and the mass ratio of honokiol to sulfisoxazole sodium in the medicine is 1:0.125-0.5; the composition can significantly reverse drug resistance of Vibrio parahaemolyticus to SMM-Na, reduce the use amount of SMM-Na, and produce a rapid sterilization effect through combined action, and meanwhile, the composition exhibits excellent protection efficacy in prawn bodies.

The application provides a method for promoting synchronous maturation of parent shrimps, which comprises the following steps: temporarily breeding male parent shrimps and female parent shrimps in a pool; adjusting water flow velocity to perform water flow domestication and temperature domestication, setting the water flow velocity to be 0.4-1.5 m / s, and feeding nutrition enhancer to perform intensive feeding when the water flow domestication is performed; the nutrition enhancer comprises the following components: taurine, arachidonic acid, phosphatidylserine and cuttlefish bone powder; setting the temperature to be 18-19 DEG C, increasing the temperature by 0.4-0.6 DEG C per day, and increasing the temperature to 27-28 DEG C. The water flow domestication and the temperature domestication are performed by adjusting the water flow velocity, and the intensive feeding of the nutrition enhancer is combined when the water flow domestication is performed, which is beneficial to the accumulation of nutritional substances in the hepatopancreas tissue of the female shrimps, promotes synchronous development and maturation of the ovary, and can make the maturation rate of the female shrimps reach 100%, and the synchronous maturation rate reach 80%.

The invention provides an ampullaria gigas-based prochloraz pesticide ecotoxicity detection method, which comprises the following steps: collecting or domesticating ampullaria gigas, and distinguishing individuals in a new hatching period and individuals in an adult period as test organisms; the method comprises the following steps: respectively exposing new hatching period pomacea canaliculata and adult period pomacea canaliculata in prochloraz aqueous solutions with different concentrations, exposing for 96 hours, and regularly observing and recording the survival individual number, the inhibited individual number and the individual number with escape behavior in each group during the period; the method comprises the following steps: exposing adult pomacea canaliculata in a series of prochloraz aqueous solutions with sublethal concentrations for 21 days, and regularly measuring and recording shell height and weight of the pomacea canaliculata bodies during exposure; taking the exposed hepatopancreas, kidney and stomach tissues of the ampullaria gigas, and carrying out histopathologic observation and analysis; the invention also provides application of the detection method in evaluating the ecotoxicity of the prochloraz pesticide. The pesticide toxicity can be rapidly and systematically tested through the easily obtained pomacea canaliculata, and the detection method is comprehensive and convenient to operate.

The application discloses a Bacillus altitudinis JYD51 and application thereof in aquaculture, which has been preserved in the China General Microbiological Culture Collection Center on October 10, 2024, and the preservation number is CGMCC No.32164. The JYD51 strain can be fermented by using a culture medium rich in organic matter, and the fermentation product has no toxic effect on Penaeus vannamei and crabs, can inhibit the infection of hepatopancreas ciliates on the Penaeus vannamei and the harm of Paramoeba on the crabs. Meanwhile, the microbial agent prepared by the JYD51 strain and Bacillus sphaericus JYQ7 can effectively control the harm of the hepatopancreas ciliates on the Penaeus vannamei and the Paramoeba on the crabs, purify the water environment for the growth of the Penaeus vannamei and the crabs, and improve the survival rate and product quality of the Penaeus vannamei and the crabs in aquaculture.

The present application relates to a kind of dsRNA of prawn SGLT1 gene and its application.The target sequence of the dsRNA is derived from the coding sequence corresponding to the transmembrane region outside loop, intracellular loop or extracellular loop of sodium-dependent glucose cotransporter SGLT1 gene coding protein.The dsRNA of prawn SGLT1 gene provides a new molecular means for preventing and controlling acute hepatopancreas necrosis disease.The dsRNA molecule can be synthesized by in vitro transcription or prokaryotic / eukaryotic expression system, and can be further constructed to contain the sequence expression cassette, recombinant vector and recombinant bacteria.The dsRNA molecule of the present application is small in molecular weight, biodegradable, and will not cause environmental pollution or drug residue, with the advantages of safety, environmental protection, high efficiency and scale production, etc., and can be used as a new biological agent or feed additive for preventing and controlling Vibrio parahaemolyticus AHPND infection.

This invention relates to the field of feed technology and provides a compound feed and preparation method for enhancing the redness of cooked river crab shells and crab roe. The feed comprises the following raw materials by weight percentage: fish meal 25-32%, defatted soybean 8-12%, blood meal 3-5%, cottonseed meal 3-5%, cottonseed meal enzymatically hydrolyzed protein 0.8-1.6%, peanut meal 16-20%, rapeseed meal 1.5-2.5%, α-starch 18-22%, soybean lecithin oil 2.5-4.0%, fish oil 1-2%, calcium dihydrogen phosphate 1.2%-1.5%, zeolite powder 0.6-1.0%, compound premix 1%, capsanthin 0.5-1.0%, zeaxanthin 0.3-0.5%, natural astaxanthin 0.1-0.2%, bile acids 0.5%, and ethoxyquinoline 0.5%. This invention utilizes the strong emulsifying properties of phospholipids to improve the bioavailability of carotenoids and enhance the redness of the hepatopancreas and crab shell; it also utilizes the phospholipid components rich in soybean phospholipid oil to promote the development of crab gonads, increase the volume of crab roe (hepatopancreas and gonads), and further enhance the overall visual redness of the crab roe through pigment accumulation during gonad development.

The invention provides a strain of Bacteroides neonati XGB-1, a bacterial agent, an aquaculture feed and an application, and belongs to the technical field of aquaculture. The Bacteroides neonati XGB-1 microbial inoculum can realize synergistic and accurate regulation and control of hepatopancreas and gonad development of the eriocheir sinensis, remarkably improves the quality consistency, gonad maturation rate and marketing efficiency of commercial crabs on the premise of not using any chemical growth promoting agent, breaks through the technical limitation of'single-point improvement and overall deficiency 'of traditional nutrient enrichment and conventional probiotics, and has a wide application prospect. And a replicable and generalizable core microbial technology path is provided for transformation of the river crab industry to high quality, safety and ecologicalization. The Bacteroides neonati XGB-1 strain provided by the invention is derived from healthy Eriocheir sinensis intestinal tracts, accords with national green aquaculture policy guidance, and has the advantages of simple production process, easily available raw materials and low cost.

The invention belongs to the technical field of biology, and particularly relates to a macrobrachium rosenbergii MrBeclin1 gene, recombinant protein and application. The autophagy related gene MrBeclin1 is cloned and identified from macrobrachium rosenbergii, and the autophagy related gene MrBeclin1 encodes proteins of 426 amino acids and has a conservative APG6 structural domain. The gene has the highest expression in hemolymph, is positioned in cytoplasm, and has obviously up-regulated expression in hepatopancreas and gill after being stimulated by aeromonas hydrophila or LPS (Lipopolysaccharide). Functional mechanism research shows that MrBeclin1 interference can cause significant change of autophagy related gene expression and cause serious pathological damage to hepatopancreas and gill. Meanwhile, the recombinant protein can be directly combined with aeromonas hydrophila, citrobacter freundii, staphylococcus aureus and streptococcus iniae, and can specifically inhibit the growth of the first two gram-negative bacteria. The invention systematically clarifies the key effect of MrBeclin1 in immune defense of macrobrachium rosenbergii through dual mechanisms of autophagy regulation and direct bacteriostasis, and provides a new molecular target and theoretical basis for prevention and control of shrimp epidemic diseases.