137 results about "Hepatopancreas" patented technology

The invention discloses a feed additive with a prawn liver protection function, in particular to a bacteria-containing feed additive prepared by culturing and fermenting bacillus subtilis, bacillus licheniformis, lactobacillus plantarum and lactobacillus casei so as to regulate internal environments in prawns and the whole water environment to alleviate hepatopancreas damage and pathological change in the prawns culture. The invention further discloses a method for preparing the feed additive. The feed additive is safe, pollution-free, residue-free, green and environment-friendly, and can be used for effectively improving the immunity and the virus resistance of the prawns, promoting the repair of chemical liver damage of the prawns, protecting livers of the prawns and effectively preventing a feeding water body from being polluted so as to increase the yield and improve the quality of the prawns; according to the method, safety and pollution-free property are achieved, the operation is simple and convenient, the production cost is low and production of various scales is facilitated.

The invention belongs to a feed, and particularly relates to a compound feed for enhancing the physiological functions of intestinal mucosa and liver/pancreas cells of fishes, which contains fish meal, soybean meal, rapeseed meal, cottonseed meal, wheat, rice bran, rice bran meal, calcium biphosphate, zeolite powder, bentonite, DL carnitine, soybean oil, vitamin-mineral premix, ursodeoxycholic acid, taurine, glutamine and yeast culture. An experimental fish raised with feed, which serves as an experimental fish for the separation and primary culture of intestinal mucosa cells and liver cells,can obtain healthy intestinal mucosa cells and healthy liver cells, moreover, the physiological condition of the experimental fish can be kept in consistency, and the repeatability of a separation and primary culture experiment on the intestinal mucosa cells and the liver cells.

The invention discloses stratiomyiid meal-containing pelteobagrus fulvidraco feed and an application thereof. The stratiomyiid meal-containing pelteobagrus fulvidraco feed comprises the following components in parts by weight: 24-40 of fish meal, 6-23 parts of stratiomyiid meal, 27 parts of soybean meal, 22-24 parts of high protein flour, 0.5 parts of fish oil, 0.2-3.4 parts of soybean oil, 0.5 part of choline chloride, 0.4 part of vitamin premix, 0.4 part of mineral premix, 1 part of phagostimulant and 1-2.5 parts of microcrystalline cellulose. The stratiomyiid meal-containing pelteobagrus fulvidraco feed has the advantages that the novel pelteobagrus fulvidraco feed is prepared by replacing part of fish meal with the stratiomyiid meal and can effectively supply all nutrient substances required for pelteobagrus fulvidraco by matching of the stratiomyiid meal and other raw materials, so that the survival rate and weight gain rate of the pelteobagrus fulvidraco can be effectively ensured; the nonspecific immune factor serum lysozyme activity, serum and hepatopancreas SOD (Superoxide Dismutase) active content and leukocyte phagocytic capacity of the pelteobagrus fulvidraco are obviously improved, and the immunity of the pelteobagrus fulvidraco is remarkably improved.

The invention discloses a freshwater shrimp vitellogenin Vg gene, an encoding protein and an application of the freshwater shrimp vitellogenin Vg gene. The full-length cDNA sequence of the Vg gene is cloned from hepatopancreas of the freshwater shrimp by a primer group provided by the invention, and the expression of a vitellogenin gene in the ovary and the liver can be silenced after the dsRNA of the gene is injected to the pericardial cavity of a female freshwater shrimp, so that the ovary development of the freshwater shrimp is remarkably delayed. The invention provides a new thought and an effective means for solving the sexual precocity phenomenon of the freshwater shrimp and culturing good varieties.

The invention discloses a novel vibrio parahaemolyticus phage with a wide lysis spectrum, amplification application thereof and specific amplification primers of the phage, and the phage has a stronglysis effect on vibrio parahaemolyticus, can be specifically amplified by host bacteria, is short in proliferation time and high in fermentation product titer, and a bacteriophage source is provided for industrial production and application of the bacteriophage. The bacteriophage can be used for preparing a medicine for resisting vibrio parahaemolyticus, and a novel medicine is provided for controlling acute hepatopancreas necrosis syndrome caused by vibrio parahaemolyticus infection. After the bacteriophage is splashed on an aquaculture water body for one time, the quantity of vibrio parahaemolyticus in the water body can be obviously reduced; the phage is mixed and fed to penaeus vannamei, or the penaeus vannamei is soaked in the phage, so that infection caused by vibrio parahaemolyticuscan be effectively prevented and treated, and the survival rate of the penaeus vannamei is increased.

The invention discloses a penaeus vannamei feed containing chlorella residue. The penaeus vannamei feed is prepared from the following raw materials in parts by weight: 24.6-29.7 parts of fish meal, 3.0 parts of meat and bone meal, 0.0-19.0 parts of soybean meal, 10.0 parts of peanut meal, 5.0 parts of dried beer yeast, 21.1-24.0 parts of flour, 2.0 parts of cuttlefish meal, 2.0 parts of shrimp shell meal, 2.2 parts of fish oil, 1.4 parts of phospholipid oil, 0.2 part of calcium bicarbonate, 0.2 part of compound vitamin, 0.8 part of compound poly-mineral and 6.0-21.0 parts of chlorella residue. According to the penaeus vannamei feed, the chlorella residue obtained by extracting biodiesel by virtue of an ultrasonic-assisted method is taken as a plant protein source of the penaeus vannamei feed, under the condition that the survival rate and the growth rate of the penaeus vannamei are guaranteed and the cost of a formula is lowered, the use amount of normal soybean meal can be decreased from 19% to 10% when the use amount of the chlorella residue is 11%, the emission amount of nitrogen and phosphorus is reduced, and the growth, the non-specific immunity enzymatic activity, the gill, the hepatopancreas and intestinal canal structure of the penaeus vannamei cannot be influenced negatively.

A feed nutritive additive for fattening eriocheir sinensis relates to an aquaculture feed additive. The additive solves the problem that provisioning food cannot meet the needs of eriocheir sinensis parental gonadal development so a feed nutritive additive for fattening is needed to guarantee that the hepatopancreas of the eriocheir sinensis can gain rich nutrition and that the eriocheir sinensis can have good gonadal development as well as have plentiful cream and fat. The technical proposal of the invention which takes phospholipid, fish oil, vegetable oil and livestock animal oil as raw materials is characterized in that the phospholipid, the fish oil, the vegetable oil and the livestock animal oil are mixed well on weight percentage of 2:1:1:1 into the nutritive additive. The invention is used as feed nutritive additive for fattening the eriocheir sinensis.

The invention discloses a mixed fodder additive capable of reducing absorption and residues of fodder heavy metals in tilapia bodies. Per kilogram of the fodder additive comprises the following components by weight: 200-500g of chitosan, 50-300g of quercetin, 25-175g of allicin, 50-200g of alpha-lipoic acid and the balance of a carrier. The mixed fodder additive can be used for reducing the absorption and residues of the fodder heavy metals in the tilapia bodies. The adding amount of the mixed fodder additive in a tilapia mixed fodder is 0.2%-4% by weight of the fodder and the mixed fodder additive can obviously reduce the level of the heavy metals in intestines, livers, muscles and an aquaculture water body of tilapia, increase the discharge of the heavy metals in excrements and prevent secondary pollution; the mixed fodder additive can also improve the antioxidant capacity of hepatopancreas and effectively relieve the growth inhibition effect of the heavy metals. The mixed fodder additive has the characteristics of easiness in obtainment of raw materials, moderate cost, safety, effectiveness, no incompatibilities, simple preparation, low adding amount and the like and further has good application prospects.

The invention discloses a nitrite nitrogen resisting trait related molecular marker of Litopenaeus vannamei and screening of the nitrite nitrogen resisting trait related molecular marker of Litopenaeus vannamei. The SNP marker remarkably related to nitrite nitrogen resistance of the Litopenaeus vannamei is obtained through carrying out a nitrite nitrogen stress experiment on the Litopenaeus vannamei, reserving hepatopancreas of surviving and dead Litopenaeus vannamei as a sample, carrying out PCR amplification by using the sample as a template, sequencing an amplified fragment so as to obtaina partial sequence of a DNA gene of the hepatopancreas and a sequenced peak chart, carrying out BLACT comparison on a sequence result so as to screen out a base mutant site, i.e., a SNP locus, then, carrying out gene subtype analysis on the sequenced peak chart by using software Seqman, and analyzing correlation between the nitrite nitrogen resistance of the Litopenaeus vannamei and a genotype byusing chi-square test of software SPSS19.0. The marker is applied to the selective breeding of the Litopenaeus vannamei and can be used for accurately and efficiently selecting the Litopenaeus vannamei with the nitrite nitrogen resisting trait for large-scale culture.

The invention discloses a novel penaeus vannamei compound feed. The novel penaeus vannamei compound feed comprises the following components in parts by weight: 15-32 parts of steam fish meal, 3-6 parts of chicken meat powder, 2-5 parts of shrimp shell powder, 3-6 parts of fermented soybean meal, 10-15 parts of peeled soybean meal, 5-15 parts of peanut meal, 2-4 parts of shrimp cream, 0.5-1.5 parts of yeast hydrolyate, 0.05-0.2 part of living antibacterial peptide, 2-5 parts of entermorpha powder, 2-5 parts of kelp powder, 15-25 parts of strong flour, 0.5-2 parts of bentonite, 0.5-1.2 parts of refined seawater fish oil, 2.0-2.5 parts of soybean lecithin, 0.1-0.5 part of composite vitamins and 1.5-2.5 parts of complex mineral salt. According to the novel penaeus vannamei compound feed disclosed by the invention, the assimilation rate of prawns to nitrogen and the absorption rate of the prawn to phosphorus can be increased, the growth of the prawns can be promoted, the unit cost can be significantly reduced, the health of the hepatopancreas and the health of the intestinal tracts of penaeus vannamei can be significantly improved, and the disease resistance of the penaeus vannamei can be obviously strengthened.

The invention discloses a special killing drug of Enterocytozoon hepatopenaei in prawns and a preparation method and application of the special killing drug. The special killing drug of Enterocytozoonhepatopenaei in prawns includes the following components: in percents by weight, 30-60% of albendazole, 10-30% of bile acid, 5-15% of Phyllanthus urinaria powder, 10-20% of artemisia apiacea powder and 5-15% of vitamin C. Application of the special killing drug comprises the steps: in percent by weight, taking the drug, mixing 1-2% of the drug with feed evenly, then spraying a diluted adhesive, performing drying for use, and performing application continuously for 2 weeks or above, wherein the drug can also be used as a feed additive, and be added into the feed at a ratio of 0.5-1% by weight.The combination and matching of the drug are reasonable and scientific, and hepatopancreas of the prawns can be protected through the synergy of the pesticide effects of the components, so that Enterocytozoon hepatopenaei in the prawns is killed, growth of the prawns is accelerated, and the immunity of the prawns is improved.

The invention provides a residue testing method for levamisole hydrochloride in different tissues of an aquatic product, and belongs to the technical field of levamisole hydrochloride detection methods. The method comprises the steps of 1, setting the basic value of an ultraviolet detector of an ultra-high performance liquid chromatograph (UPLC); 2, extracting the residue of levamisole hydrochloride in the plasma of an aquatic product; 3, extracting the residues of levamisole hydrochloride in the muscle, the hepatopancreas and the kidney of the aquatic product; 4, determining the content of levamisole hydrochloride in the aquatic product. According to the technical scheme of the invention, pre-treatment technologies are optimized and rationally combined, so that the extraction and purification effect is ensured to be good. Meanwhile, the condition of the UPLC is explored and confirmed, and the sensitivity, the repeatability and the accuracy of the detection method are improved. Especially, the detection and analysis speed is accelerated by several times, and the reagent waste is lowered. The environmental pollution is reduced. The method is suitable for the residue detection of great batch of samples.

The invention discloses a MnRXR gene of macrobrachium nipponensis, and an amplification primer group and an amplification method thereof. A full-length cDAN sequence of the MnRXR gene is cloned from the hepatopancreas tissue of macrobrachium nipponensis. The gene comprises two variable spliceosomes: MnRXR-L and MnRXR-S. The invention lays an important base for research on functions of the MnRXR gene of macrobrachium nipponensis, in particularon important effects in a moult cycle.

The invention discloses an eriocheir sinensis HSP90 gene clone and application thereof. The gene has a base sequence as shown in SEQ ID NO.1. The protein encoded by the gene has an amino acid sequence as shown in SEQ ID NO.2. The HSP90 gene provided by the invention is firstly obtained from eriocheir sinensis hepatopancreas by amplification, can be used for in vitro recombination expression and gene transfer of the HSP90 gene, and lays a foundation for developing medicine products, selected genetic breeding and improving, and enhancing stress resistance to high temperature, low temperature, heavy metal ion exceeding, pathogenic bacterial infection and the like.

The invention relates to a trypsin gene cDNA sequence of Scylla paramamosain and a cloning method and application thereof. The trypsin gene cDNA sequence of Scylla paramamosain has the sequence shown in SEQ ID NO:1; the cloning thereof comprises the following steps: extracting total RNA from the hepatopancreas of the Scylla paramamosain; building a cDNA plasmid library; carrying out authentication and sequencing analysis on the cDNA plasmid library; and according to the BLAST comparison, obtaining the sequence shown in SEQ ID NO:1. The trypsin gene cDNA sequence of the Scylla paramamosain can forecast feeding amount and exuviation cycle in the germchit and reproductive process of blue crabs so that people can master the life cycle of the blue crabs and select and use in inheritance breeding, reduce the bait consumption, improve the germchit survival rate, thereby optimizing the process of grow seedlings.

The invention relates to a protein yolk antibody for immunologic diagnosis of acute hepatopancreas necrosis syndrome, and a preparation process and application thereof. The protein yolk antibody uses pirA and pirB protein as antigen immune egg laying poultry and is extracted from the yolk of eggs; the specific binding with the pirA and pirB protein can be realized. The prepared protein yolk antibody has good activity, good specificity, good stability and high hypertonicity resistance. Meanwhile, a preparation method provided by the invention has the characteristics of high benefit, safety, low cost, environment-friendly effect, high prevention performance and the like; the method provides an effective path for prevention, treatment and detection of the acute hepatopancreas necrosis syndrome.

The invention discloses an anti-stress immunopotentiator for a Chinese mitten crab juvenile crab and a preparation method and application thereof. The method is as follows: taking fructooligosaccharides, lactic acid bacteria, Bacillus subtilis and yeast; respectively crushing the fructooligosaccharides, the lactic acid bacteria, the Bacillus subtilis and the yeast with a superfine crusher, and then sieving with a 120 mesh screen; evenly fully mixing the lactic acid bacteria, the Bacillus subtilis and the yeast in a mixer according to the proportion, and mixing the fructooligosaccharides with the lactic acid bacteria, Bacillus subtilis and yeast mixture evenly to obtain the powdery anti-stress immunopotentiator. The anti-stress immunopotentiator has the advantages of stable sources of raw materials, wide range of raw materials, simple preparation and high cost performance; the anti-stress immunopotentiator is based on the immune mechanism of crustaceans, and is helpful to improve the antioxidant capacity of crab hepatopancreas, remove free radicals in body, improve immunity and anti stress ability of the body, and promote the healthy growth of the juvenile crab when in use.

The invention discloses a method for controlling prawn hepatopancreas necrosis by polyculture of scatophagus argus and marsupenaeus japonicas. The method comprises: in breeding, throwing marsupenaeus japonicas shrimp seeds whose total length is 0.8-1.0 cm, throw-in density being 70-80 thousands seeds in each mu, when the shrimp seeds growing to 2-3 cm of marsupenaeus japonicas body length, throwing scatophagus argus whose body length is 3-5 cm, throw-in volume being 200-300 fish/mu. Through throwing the scatophagus argus which has omnivory and benthic feeding habit in proper density, proper specification, prawn culture pond water body and bottom pollution can be reduced, so as to improve culture pond environment, reduce harmful bacteria contents in the pond water and bottom mud, and control occurrence and diffusion of bacterial diseases in prawn culture. The method can effectively eliminate redundant nutrient substances in the bottom of the pond and in the water, reduces bacteria number, prevents outburst of prawn hepatopancreas necrosis, and improves success rate of culture.

The invention relates to a method for evaluating the water quality by use of an MT (metallothionein) gene expression mode of freshwater crayfish, belonging to the field of environmental biotechnology. The method is used for evaluating the water quality of industrial sewage by use of the MT gene expression mode of freshwater crayfish. By adopting the method provided by the invention, the MT gene is cloned from the hepatopancreas tissue of the freshwater crayfish for the first time, an industrial sewage stress experiment proves that the gene is related to the physiological reaction of the freshwater crayfish against water environment stress, and the expression mode of the MT gene of the freshwater crayfish after sewage stress can reflect the degree of water pollution. Therefore, the invention provides a comprehensive and quick technological means for evaluating the quality of the polluted water as well as the existing potential ecotoxicological effect by use of a biotechnological method, and has relatively broad application prospects in terms of environmental protection.

The invention discloses a macrobrachium rosenbergii compound feed using a daphnia magna dry powder for partially replacing fish meal. According to the macrobrachium rosenbergii feed, the daphnia magna dry powder is used for replacing 21.7 percent to 44.0 percent of fish meal. The optimum dosage of the daphnia magna dry powder is 5 percent to 20 percent, and the dosage of the fish meal is reduced to 16.8 percent to 26.7 percent from 30 percent. The experiment proves that compared with non-replaced fish meal control groups, macrobrachium rosenbergii hepatopancreas SOD, serum MDA, serum glutamic pyruvic transaminase, serum glutamic oxalacetic transaminase in replacement groups are lower than those in the control groups in different degrees, so that by using the daphnia magna dry powder for replacing the fish meal, the macrobrachium rosenbergii can have a better anti-oxidative stress capability, and is more beneficial for keeping the health of hepatopancreas.

The invention belongs to the technical field of nondestructive testing, and relates to a nondestructive testing method for the internal eating quality of Eriocheir sinensis based on a smart phone. The method comprises the following steps: taking Eriocheir sinensis in different mature periods as samples, acquiring spectral data of crab meat, hepatopancreas, female crab gonad and male crab gonad ROI of the samples by utilizing a spectral data acquisition system based on the smart phone, and measuring the contents of characteristic chemical substances of the samples; carrying out SG smooth filtering preprocessing on the spectral data; according to the maturity of the samples, combining with the characteristic chemical substance content of crab meat, hepatopancreas, female crab gonad and male crab sex to formulate the grading index of the internal eating quality of the Eriocheir sinensis; enabling the preprocessed spectral data to be in one-to-one correspondence with the characteristic chemical substance content data, and obtaining the characteristic wavelength corresponding to each part of the sample through an algorithm; constructing a prediction model according to the characteristic wavelength data and the edible quality grading indexes. The method has the advantages of accuracy, high efficiency, no damage and real-time performance, and makes up the vacancy of the grading standard of the internal edible quality of the commercial crabs.

The invention relates to a red-increasing feed additive for parrot fish. The additive comprises capsanthin and vitamin E in a weight ratio of (7-15):(3-10). According to the invention, capsanthin and vitamin E which are adopted as raw materials are mixed into common feed in the ratio, and then the feed is conventionally used for feeding. Experiments prove that the surface of the parrot fish is obviously colored after the use of the additive, and the coloring effect is far greater than that of a control group; activities of glutamic-pyruvic transaminase and glutamic oxalacetic transaminase are not influenced so that no side influences occur to liver-pancreas of the parrot fish; moreover, the weight increment of the parrot fish and the activities of antioxidant indexes in the liver-pancreas are obviously influenced; in conclusion, the additive can effectively increase the color of the surface of the parrot fish and promote the growth of the parrot fish and has good effect in the increment of the antioxidant ability of the parrot fish and the accumulation of carotenoid.

The invention relates to a feed additive capable of improving the antioxidant function and reproductive performance of macrobrachium nipponensis. The feed additive is prepared from, by weight, 15-27 parts of radix isatidis, 17-25 parts of astragalus polysaccharides, 4-8 parts of flos lonicerae, 4-8 parts of fructus rosae laevigatae, 4-13 parts of radix et rhizoma rhei, 8-15 parts of rhizoma calami, 7-15 parts of shrimp shell meal, 4-9 parts of shell powder and 16-22 parts of yolk immunoglobulin. The feed additive has the advantages that 2-8% of the feed additive is stirred in basic feed of which the protein content is 37.4%, when the water temperature rises to 10 DEG C in March, feeding is started, feeding is carried out twice each day, continuous feeding is carried out for 1 month, thereby being capable of not only improving the growth speed of macrobrachium nipponensis and the antioxidant function of the hepatopancreas, but also promoting the development of the ovary of female shrimps; the problems that a macrobrachium nipponensis female parent is slow in growth speed, low in disease resistance and slow in ovary development in the traditional macrobrachium nipponensis breeding process are solved, the additive amount is low, and the preparation method is simple.