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Method for evaluating water quality by use of MT (metallothionein) gene expression mode of freshwater crayfish

A technology for freshwater crayfish and gene expression, applied in biochemical equipment and methods, microbe measurement/inspection, fluorescence/phosphorescence, etc., can solve problems such as single detection means and inability to fully explain potential biological safety problems of polluted water bodies , to achieve the effect of simple technical operation

Inactive Publication Date: 2013-12-18
INNER MONGOLIA UNIV OF SCI & TECH
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Problems solved by technology

[0005] For a long time, environmental protection departments have mostly used chemical detection methods to determine whether the water body is polluted. This method has a single detection method and cannot fully explain the potential biological safety problems of the polluted water body. From a scientific point of view to reasonably explain the ecotoxicological effects of water pollution

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  • Method for evaluating water quality by use of MT (metallothionein) gene expression mode of freshwater crayfish
  • Method for evaluating water quality by use of MT (metallothionein) gene expression mode of freshwater crayfish

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Embodiment 1

[0019] Water Quality Evaluation and Potential Ecotoxicological Effect Analysis of Seepage Sewage from a Metal Smelting Tailings Pool

[0020] In Figure 1, the value of the ordinate is 2 -ΔCt , the value on the abscissa is time, and * indicates that this value is significantly different from the normal control group.

[0021] 1) Water sample pretreatment: take 5L-10L of seepage sewage (raw water) from a metal smelting tailings pond for backup, dilute it with distilled water 5 times and divide it into 3 parts on average (as 3 groups of parallel experiments), and obtain 3 groups of water samples ;

[0022] 2) Crayfish stimulated by sewage stress: put freshwater crayfish with a weight of 20g-30g into the three groups of water samples in step (1), and raise 30 crayfish in each group of water samples, and the sampling time point is after stress stimulation 6h, 12h, 24h, 48h, 72h, note: the crayfish sampled each time must be live crayfish, dead crayfish are not used as the research...

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Abstract

The invention relates to a method for evaluating the water quality by use of an MT (metallothionein) gene expression mode of freshwater crayfish, belonging to the field of environmental biotechnology. The method is used for evaluating the water quality of industrial sewage by use of the MT gene expression mode of freshwater crayfish. By adopting the method provided by the invention, the MT gene is cloned from the hepatopancreas tissue of the freshwater crayfish for the first time, an industrial sewage stress experiment proves that the gene is related to the physiological reaction of the freshwater crayfish against water environment stress, and the expression mode of the MT gene of the freshwater crayfish after sewage stress can reflect the degree of water pollution. Therefore, the invention provides a comprehensive and quick technological means for evaluating the quality of the polluted water as well as the existing potential ecotoxicological effect by use of a biotechnological method, and has relatively broad application prospects in terms of environmental protection.

Description

technical field [0001] The invention relates to a freshwater crayfish MT gene expression pattern method for evaluating water body quality, which belongs to the field of environmental biology technology. Background technique [0002] With the rapid development of my country's economy, governments at all levels from the central to local governments attach great importance to environmental protection. In the process of solving the contradiction between rapid economic development and increasingly serious environmental protection problems, governments at all levels have formulated very strict rules and regulations and treatment measures, especially taking water environmental protection as the primary task. Judging from the major environmental and residents' health problems caused by frequent water pollution over the years, it is imperative to protect the water environment. Judging from the frequent water pollution caused by malicious sewage discharges by enterprises and the shar...

Claims

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Application Information

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IPC IPC(8): C12Q1/68G01N21/64
Inventor 杜志强张雪峰王建英
Owner INNER MONGOLIA UNIV OF SCI & TECH
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