45results about How to "Good survival rate" patented technology

It is intended to provide Bifidobacterium bifidum which has an effect of killing Helicobacter pylori and shows high survivability even in the case of being stored in a fermented milk food or drink under aerobic condition. The Bifidobacterium bifidum has the following characteristics: (1) having an effect of killing Helicobacter pylori; and (2) showing a survival rate of 10% or higher in the case of being stored in a fermented milk drink or food under aerobic condition at 10° C. for 14 days.

The invention discloses an antibiotic-free conservation fodder for piglets. The conservation fodder comprises the following components in part by weight: 550-640 parts of maize, 40-60 parts of super steam fish meal, 200-250 parts of peeled soybean meal, 40-60 parts of whey powder, 20-30 parts of glucose, 1-2 parts of table salt, 5-9 parts of secondary calcium phosphate, 10-25 parts of Nengbao, 7-12 parts of calcium formate, 10 parts of premix of the conservation fodder for piglets, 6-11 parts of lysine, 1 part of acidifying agent (coated), premix of vitamins, premix of compound minor elements for piglets, phytase, multienzyme, antibacterial peptide, prebiotics, antioxidant and zeolite powder. The antibiotic-free conservation fodder for piglets does not contain any antibiotics, has no defect of drug resistance of animals, drug residues, environmental pollution and the like, and can rival with or even exceed the antibiotic-contained piglet conservation fodder in the prior art in controlling diarrhea and promoting growth of the piglets.

The invention relates to a litopenaeus vannamei culture method based on a microorganism-aquatic plant-marine fish multistage biological system, wherein high-density culture without changing water canbe achieved. According to the method, a microorganism, aquatic plant and fish multistage culture system is built, staged high-density culture of litopenaeus vannamei is implemented, two stages includea high-density culture stage of a young litopenaeus vannamei pond and an amplification culture stage of a large pond in the development period of young litopenaeus vannamei, the survival rate and theyield of the litopenaeus vannamei are increased, and ecological benefits, economic benefits, resource conservation and the like in a culture system are remarkable.

The invention discloses a nanometer sustained-release compound aquaculture feed and a feeding method thereof. The nanometer sustained-release compound aquaculture feed is prepared from the following component in parts by weight: 30-35 parts of high-protein-content whole potato powder. The nanometer sustained-release compound aquaculture feed and the feeding method thereof are complete in protocol and simple in technology. The feeding technology and the rationally matched multiple raw materials in the feed are coordinated, so that the nutrients in the raw materials are mutually complementary; thus, the feed is high in protein content. The chitosan is a kind of natural macromolecular polysaccharide, and has the performance characteristics that the chitosan is wide in source, non-toxic, tasteless, good in biocompatibility, good in biodegradation, and good in antibacterial properties and so on. Thus, the feed is rich and comprehensive in nutrients, good in palatability, low in feed coefficient, and capable of improving immunity and antioxidant capacity of the tinca tinca. Moreover, the tinca tinca fed with the feed is high in survival rate.

The invention discloses a tissue culture method for a succulent plant. The method comprises the steps that 1, leaves of the succulent plant are sterilized and disinfected and then chopped as an explant, and the explant is placed in a basic culture medium for primary induction to obtain callus; 2), the callus is placed in an induction culture medium for secondary induction to promote generation ofat least two adventitious buds; 3, the callus with the adventitious buds is cut to obtain callus blocks, and the callus blocks are placed in a proliferation culture medium for proliferation culture toobtain proliferation bud blocks; 4, the proliferation bud blocks are placed in a rooting culture medium for rooting culture to obtain tissue culture seedlings; 5, the tissue culture seedlings are subjected to seedling hardening. The tissue culture method of the succulent plant has high survival rate and proliferation rate.

The invention relates to a method for manufacturing a seaweed seed bottom laying layer. The method comprises the steps that seaweed seeds are collected and pretreated; the pretreated seaweed seeds and a matrix are uniformly mixed to form a mixture; net cloth is selected as a bottom laying layer net frame and folded to form the double-layer bottom laying layer net frame, and the double-layer bottom laying layer net frame is sewn with twine in an equally spaced mode so that multiple interlayers of the double-layer bottom laying layer net frame can be formed; the interlayers of the net frame are filled with the mixture from the open end of the net frame, the open end is sewn with twine, and then the seaweed seed bottom laying layer is formed; the manufactured seaweed seed bottom laying layer is placed in a water tank, transported to a bottom laying site and directly put into the sea. Materials adopted in the method are all natural materials and can gradually degrade after being planted underwater without causing secondary pollution to the environment. The method facilitates planting, laying, management and maintenance of seaweed.

The invention discloses a micro-nano composite structure surface matched with circulating tumor cell size, a preparation method and application thereof, and belongs to the technical field of the biologics. The preparation method comprises the following steps: preparing a micro-nano composite structure array matched with a to-be-detected tumor cell size on the surface of the substrate, and then fixing specific antibody on the tumor cell surface on the surface of the structure area, utilizing the structure array to synergistically effect with the specific antibody to realize the efficient and specific capturing on the circulating tumor cell. The theory of designing the micron/nanometer structure is designed according to the target cell size can be further used for detecting other cells, thereby providing a new path for the preparation of the biological detection substrate. The substrate prepared through the invention can efficiently and specifically capture the circulating tumor cell, and has the features of being simple in preparation and low in cost; the captured cell has good survival efficiency, and a new path is provided for the preparation of the biological detection substrate.

The invention provides a cuttage cultivation method for picria fel-terrae lour. According to the method, the tip portion of the picria fel-terrae lour which is good in growth condition and free of the disease and pest damage is obtained and directly inserted in the soil in a cuttage mode, a callus is formed in the cuttage portion, then rooting is achieved and the plant of the picria fel-terrae lour is obtained. According to the method, a high survival rate and a high growth rate of cultivation of the picria fel-terrae lour can be guaranteed.

The invention discloses a fresh ginger tissue culture method which comprises the following steps of 1) taking fresh ginger shoot tips as explants to perform bacterium removal with heat treatment, thenputting a product in an antibiotic solution for secondary sterilization, then putting a product in an initial medium for induced culture and in a multiplication medium for multiplication culture to obtain differentiated seedlings; 2) performing rooting culture on the differentiated seedlings in a rooting medium to obtain a tissue culture seedlings; and 3) seedling hardening on the tissue cultureseedlings, wherein the culture conditions of the step 1 to the step 2 at least accord with the following conditions: the condition is aseptic and air permeable; the initial medium is an MS culture medium containing indoleacetic acid (IAA), 6-benzyladenine 6-BA and folic acid; the multiplication culture medium is an MS culture medium containing zeatin ZT, streptomycin, 2,4-D and a watermelon juice;and the rooting medium is a 1/2 MS culture medium containing paclobutrazol, indole butyric acid (IBA) and mashed potatoes. The fresh ginger tissue culture method has excellent survive rate and reproductive rate.

The invention discloses a parthenocissus quinquefolia culturing box, a slope greening system and a greening method. Parthenocissus quinquefolia is cultured to a mature stage through the culturing box in advance. The culturing box is integrally fixed to a slope. The parthenocissus quinquefolia can cover the whole slope after growing up. The whole growth covering process is quick and simple, the spray-seeding process is omitted, and the construction speed is high. The parthenocissus quinquefolia survival rate is high, manual management is not needed after the parthenocissus quinquefolia is planted, and the parthenocissus quinquefolia culturing box, the slope greening system and the greening method are quite suitable for quickly greening slopes, especially large-angle bared rock slopes, cliffs of exploited mines and various bridge piers.

The invention discloses a tissue culture method for pineapples. The tissue culture method comprises the following steps: (1) sterilizing and disinfecting crown buds of the pineapples, cutting the crown buds up to serve as an explant, putting the explant into a basic culture medium for primary induction, so as to obtain a callus; (2) putting the callus into an induction culture medium for secondaryinduction, so as to hasten the generation of adventitious buds; (3) dicing the callus with the adventitious buds so as to obtain a callus block with at least two adventitious buds, putting the callusblock into a proliferation culture medium for proliferation culture, so as to obtain a proliferated bud block; (4) putting the proliferated bud block into a rooting culture medium for rooting culture, so as to obtain a tissue culture seedling; and (5) hardening the tissue culture seedling. The tissue culture method for the pineapples has excellent survival rate and reproduction rate.

The invention provides a method for papaya potting in north China. The method comprises the following steps of a. nutrient soil preparing, wherein nutrient soil comprises 40%-50% of turfy soil, 10%-20% of vermiculite, 5%-15% of surface soil, 10%-15% of perlite and 10% of decomposed manure; b. planting, wherein planting time is the last ten-day period of April, a potting container is filled with the nutrient soil with the depth of 25 cm-35 cm, and water is fully added after papaya seedlings are planted; c. water management, wherein watering is carried out every 5-7 days in spring, every 2-3 days in summer, every 4-5 days in autumn and every 6-8 days in winter, and 10 kg-20 kg of water is added each time; d. manure management, wherein in a quick-growing period in July, capillary roots exposed out of the ground surface are cover by the nutrient soil by 2 cm-3 cm, and 50 g of compound fertilizer is applied; and e. temperature management, wherein temperature in summer is 26-32 DEG C, and temperature in winter is higher than 15 DEG C for heat preservation. According to the method, potting of papayas with good growth vigor can be carried out in north China.

The invention belongs to the technical field of plant seedling reproduction, and discloses a cutting cultivation method for wax begonias. The cutting cultivation method includes selecting one-year-oldhealthy twigs, free from plant diseases and insect pests, with terminal buds, short internodes, strong sprouts and branches, cutting the twigs, pinching off lower leaves, and flattening the lower sides of node orders of bases of the twigs by a blade; soaking the lower portions of cuttings in a composite solution for more than 30 seconds; inserting the cuttings into holes after soaking, and watering the cuttings after compacting; covering the cuttings with plastic bags with holes for moistening, selecting a shading net with the light transmittance of 70% for lighting management, checking rooting conditions, and performing transplant. The cutting cultivation method has the advantages of vigorous growth of root systems, high quantity of roots and high survival rate.

The invention provides a cutting seedling culture method of pileostegia viburnoides under subtropical high-altitude climatic conditions. The cutting seedling culture method of pileostegia viburnoidesunder subtropical high-altitude climatic conditions comprises the following steps of (1) preparing cutting substrates: uniformly mixing peat soil and yellow soil to obtain mixtures to be used as the cutting substrates; (2) preparing cutting panicles; (3) performing soaking with rooting powder: soaking the cutting panicles cut in the step (2) in heteroauxing rooting powder; (4) performing cutting;(5) performing moisture management; and (6) performing illumination management. The cutting seedling culture method of pileostegia viburnoides under subtropical high-altitude climatic conditions disclosed by the invention has the advantages that the pileostegia viburnoides can be well planted at high-altitude low-temperature regions, the cutting seedling breeding speed is high, the cutting seedling culture method of pileostegia viburnoides under subtropical high-altitude climatic conditions is labor-saving, effortless and environmentally-friendly, pileostegia viburnoides seedlings are easy tosurvive after being transplanted, and the cutting seedling culture method of pileostegia viburnoides under subtropical high-altitude climatic conditions has favorable repairing effects on vegetation destruction caused by manual activities of way building, mine exploiting and the like at subtropical mountain massifs.

The invention relates to the technical field of seedling breeding, in particular to a Portuguese oyster triploid artificial seedling breeding technology, which comprises the following steps: hybridizing cultivated crassostrea gigas tetraploid and cultivated Portuguese oyster diploid to obtain Portuguese oyster triploid fertilized eggs, cultivating the Portuguese oyster triploid fertilized eggs to juvenile mollusks, and culturing the juvenile mollusks to obtain the Portuguese oyster triploid artificial seedling. The juvenile mollusks are cultivated into Portuguese oyster triploid adults; proper temperature and salinity are provided for culture of the Portuguese oyster triploid, and the cultured Portuguese oyster triploid has a good survival rate.

The invention discloses a tissue culture method of trichosanthes kirilowii, which includes: 1) performing thermal treatment sterilization to trichosanthes kirilowii stem sections being an explant, performing secondary sterilization in alcohol solution, and performing induction culture in a primary culture medium and then proliferation culture in a proliferation culture medium to obtain differentiated seedlings; 2) performing rooting culture on the differentiated seedlings in a rooting culture medium, then performing low temperature treatment at lower than 5 DEG C to obtain tissue culture seedlings; 3) exercising the tissue culture seedlings. The primary culture medium is a MS culture medium containing naphthylacetic acid (NAA), 6-benzyladenine (6-BA) and inositol; the proliferation culturemedium is a MS culture medium containing zeatin (ZT), kanamycin, 2,4-D and tomato juice; and the rooting culture medium is a 1/2 MS culture medium containing paclobutrazol, indolebutyric acid (IBA) and smashed banana. The method has excellent survival rate and reproductive rate.