The invention discloses a non-damage preparation method for single
crassostrea hongkongensis. The preparation method comprises the following steps: carrying out
seedling collection by adopting a
crassostrea angulata left shell as an adhering substrate, enabling seedlings of the
crassostrea hongkongensis to be adhered onto the adhering substrate, and then carrying out
seedling cultivation till the seedlings grow to be 30-60mm, so as to obtain adhering substrate combined clustered seedlings; placing the adhering substrate combined clustered seedlings into a culture cage, transferring the culture cage with the seedlings into a
water body substrate-removing environment of which the
salinity is 3-9 ppt, and the pH value is 6.00-6.75, and obtaining the single
crassostrea hongkongensis after the crassostrea angulata left shell is molten. According to the invention, as the
crassostrea hongkongensis has the characteristic of being capable of adapting to extreme environments with
low salinity, low pH value and the like, the crassostrea angulata left shell is adopted as the adhering substrate, when the seedlings of the
crassostrea hongkongensis grow to be 30-60 mm, the seedlings and the adhering substrate are placed in the culture cage, the culture cage is transferred into the environment with
low salinity and low pH value, the cultivation is carried out for 15-30d, the adhering substrate is spontaneously and gradually molten through the
ocean acidification reaction between slight acid
brackish water and the crassostrea angulata left shell, the single crassostrea hongkongensis is prepared and scattered in the culture cage, and then, the single crassostrea hongkongensis is transferred to a proper culture area for continuing the cultivation.