63 results about "Crassostrea" patented technology

The invention discloses a cultivation method of a novel strain of crassostrea gigas with a pure purple left shells. The cultivation method is characterized in that operation comprises the steps of: (1) seed production: selecting male and female individuals with pure purple left shells from a cultured group as cultivating parent shellfishes; performing one-to-one insemination and copulation, respectively marking breeding systems, cultivating larva by adopting strict isolation measures, and placing in a same sea area for intermediate culture and cultivation; breeding generation by generation to purify so as to form a family; and (2) purification of the novel strain: selecting inseparate families with character proportion of above 90% as parent shellfishes, performing seedling propagation so as to cultivate the novel strain of crassostrea gigas with the pure purple left shells and stable character. With the adoption of the cultivation method provided by the invention, the cultivating process of the novel strain is accelerated and transgenic and pharmaceutical treatment do not need to be carried out, the novel strain is cultivated, the additional value of product is also improved through color character, and the market sale price and income of cultivation enterprises and cultivators are increased, and market prospect is wide.

The invention relates to a seed producing method for obtaining the hybrid advantage of a crassostrea gigas, which is characterized by comprising the following steps: selecting Chinese and Korean crassostrea gigases as parent oysters to carry out temporary culture; ripening the Chinese and Korean crassostrea gigases synchronously; then hybridizing sperms and ovums of the Chinese and Korean crassostrea gigases by artificial insemination; and finally carrying out the incubation, the seed culture and the growth of the hybridized oyster. The invention cultures a crassostrea gigas hybridized seed with high disease resistance and survival rate and rapid growing speed by hybridizing two crassostrea gigas geographic populations with reproductive isolation; the production performance of the crassostrea gigas hybridized seed is obviously better than that of a Chinese locally inbred comparison population; and the method is simple and has favorable industrialized application prospect.

The present invention relates to a PCR-RFLP identification method for 7 kinds of Chinese south inshore oysters. Said identification method includes the following several main steps: making genome DNA extraction, PCR amplification of object fragment, selecting proper incision enzyme combination, utilizing selected incision enzyme to enzyme-cut the PCR product and utilizing agarose gel electrophoresis to make detection.

The invention provides a substratum used in an artificial breeding and production process of crassostrea gigas and a manufacturing method of the substratum, and provides a method for cultivating the crassostrea gigas by using the substratum. The manufacturing method of the substratum comprises the following steps: cutting and bundling polypropylene packing straps; then wrapping the outer surface of the polypropylene packing straps with mixed cement paste; and air-drying the polypropylene packing straps to obtain the substratum. The mixed cement paste comprises the following components in percentages by mass: 0.5-1% of epoxy resin, 10-15% of fine sand, 15-20% of shell powder and the balance of cement; and the total mass percentage is 100%. The substratum is made of materials obtained easily, is low in cost and high in strength, and resists stretching and seawater corrosion. By the manufactured substratum, seedlings are collected easily, and the seedling attachment effect is good. Compared with frequently used chlamys farreri, the substratum is better in effect.

The invention discloses a breeding method for a new strain of crassostrea gigas with orange left and right shells. The breeding method comprises the following steps of selecting individuals with purple left shells and black palliums from a cultured population to be used as male reproduction brood stocks; selecting individuals with black left shells and black palliums to be used as female reproduction brood stocks; carrying out monogamous insemination to establish an F1 family; selecting female and male individuals with black left and right shells and black palliums from the F1 to be used as reproduction brood stocks and establishing an F2 family; continuously selecting female and male individuals with purple black left and right shells and black palliums from the F2 to be used as reproduction brood stocks and establishing an F3 family; selecting orange individuals as parents of the new strain from the F3 and propagating offspring seeds to culture the new strain of the crassostrea gigas with the orange left and right shells and stable characters. The additional value of products is improved and the market prospect is broad.

The invention discloses a breeding method of crassostrea gigas (black oysters) with a high melanin content in the soft parts and a proof scheme for the effectiveness of the method. The breeding method includes the following steps that by observing the color of common oysters, the oysters of which the ratio of the area of black shells to the total shell area is more than 90 percent and the lightness value V< 0.20 are selected, melanin extraction of oyster shells, melanin extraction of soft parts of the oyster shells and relation analysis of the melanin content and the melanin content of the soft parts are carried out, the applicant finds a significant positive correlation phenomenon of the color of the shells of the crassostrea gigas and the deposit rate of melanin in the pallium in the experiment process, and the blacker the shells are, the higher the melanin content of the soft parts is. By means of the breeding method of the crassostrea gigas (black oysters) with the high melanin content in the soft parts, the oysters with the high melanin content in the soft parts can be selected and bred rapidly, the breeding efficiency of the oysters is improved, and as the health-care value of melanin is accepted by the market, the new oyster species obtained through the method can increase the additional value of breeding.

The invention discloses a method for quickly detecting the content of glycogen and protein of crassostrea gigas. The method comprises the following steps: firstly, homogenizing a to-be-detected sample; then, collecting near infrared spectrum data; and finally comparing with a pre-established crassostrea gigas glycogen and protein content near infrared model to acquire a measurement value of the to-be-detected sample. The method has the characteristics of high detection speed, no chemical reagent, low experiment cost and no environmental pollution in the process of analyzing the content of glycogen and protein of crassostrea gigas. Establishment of the method for quickly detecting content of glycogen and protein of crassostrea gigas has important meaning to crassostrea gigas meat quality analysis, meat property breeding, breeding generation identification and genetic resource evaluation.

The invention discloses a non-damage preparation method for single crassostrea hongkongensis. The preparation method comprises the following steps: carrying out seedling collection by adopting a crassostrea angulata left shell as an adhering substrate, enabling seedlings of the crassostrea hongkongensis to be adhered onto the adhering substrate, and then carrying out seedling cultivation till the seedlings grow to be 30-60mm, so as to obtain adhering substrate combined clustered seedlings; placing the adhering substrate combined clustered seedlings into a culture cage, transferring the culture cage with the seedlings into a water body substrate-removing environment of which the salinity is 3-9 ppt, and the pH value is 6.00-6.75, and obtaining the single crassostrea hongkongensis after the crassostrea angulata left shell is molten. According to the invention, as the crassostrea hongkongensis has the characteristic of being capable of adapting to extreme environments with low salinity, low pH value and the like, the crassostrea angulata left shell is adopted as the adhering substrate, when the seedlings of the crassostrea hongkongensis grow to be 30-60 mm, the seedlings and the adhering substrate are placed in the culture cage, the culture cage is transferred into the environment with low salinity and low pH value, the cultivation is carried out for 15-30d, the adhering substrate is spontaneously and gradually molten through the ocean acidification reaction between slight acid brackish water and the crassostrea angulata left shell, the single crassostrea hongkongensis is prepared and scattered in the culture cage, and then, the single crassostrea hongkongensis is transferred to a proper culture area for continuing the cultivation.

The invention provides a new ostrea rivularis species breeding method. Firstly, ostrea rivularis and crassostrea gigas parents are selected, and oyster parent ripening promotion breeding is conducted; secondly, ostrea rivularis and crassostrea gigas are manually dissected, female ostrea rivularis and male crassostrea gigas are selected in a microscopic examination mode, and eggs of ostrea rivularis and sperms of crassostrea gigas are taken out for fertilization to form hybrid fertilized ovums of ostrea rivularis; thirdly, after incubation of the hybrid fertilized ovums, larvae are selected and bred in a larva breeding pond; finally, settlement and metamorphosis are conducted on the bred larvae, and a first generation of hybrid fry of ostrea rivularis for breeding is bred, and through artificial breeding, new ostrea rivularis species which is high in disease resistance and growing speed and good in meat quality is screened out to be bred.

The invention discloses a microsatellite multiplex-PCR (Polymerase Chain Reaction) method for carrying out a paternity testing on crassostrea gigas. The microsatellite multiplex-PCR method comprises the following steps of extracting parent and offspring DNA (Deoxyribose Nucleic Acid); carrying out multiplex-PCR amplification on extracted DNA samples by using 18 microsatellite sites and M13 (-21) universal primers with fluorescent marks; detecting a fluorescent signal of an amplified product, and obtaining parent and offspring gene type data; carrying out genetic relationship identification on the parent and the offspring. The microsatellite multiplex-PCR method disclosed by the invention verifies that two groups of multiplex-PCR combinations are used for carrying out the paternity testing on 12 crassostrea gigas full-sib families, so that the identification success rate is up to 100 percent; a set of DNA mark-based paternity testing technology which is economic and effective and is high in accuracy is provided for the crassostrea gigas; by a genealogical relationship record obtained through microsatellite mark identification, producing places of individual crassostrea gigas can be traced back, so that a tracking and source tracing technology of crassostrea gigas products can be established, and powerful technical support can be provided for solving food safety problems which are increasingly concerned by people in nowadays society.

The invention belongs to the technical field of molecular biology and particularly relates to application of crassostrea gigas C-type agglutinin-4 (CgCLec-4).According to the method, an in vitro recombination expression technology is utilized, recombination protein of a carbohydrate recognition domain (CRD) of crassostrea gigas CgCLec-4 is obtained, and application of crassostrea gigas C-type agglutinin-4 (CgCLec-4) recombination protein to preparation of a bacteriostatic agent or a fodder additive is obtained.The obtained recombination protein can be used for development of an antibacterial fodder additive or novel immunopotentiator.

The invention relates to a preparation method of crassostrea gigas meat antifatigue nutrient solution. Crassostrea gigas meat is smashed into pulp, ethyl acetate is added and stirred for removing fishy smell, centrifugation is carried out, ethyl acetate is removed, crassostrea gigas meat pulp is collected, water is added, stirring to be uniform is carried out, heating is carried out, neutral protease is added according to the mass of crassostrea gigas for enzymolysis, the neutral protease is activated, papain is added according to the mass of crassostrea gigas for enzymolysis, the papain is activated, then bromelain is added according to the mass of crassostrea gigas for enzymolysis, and then the bromelain is activated, and cooling to room temperature is carried out. Centrifugation is carried out, water solution is collected, vacuum concentration is carried out, sterile water is used for diluting to appropriate concentration, sugar, citric acid and edible salt are added, and mixing to be uniform is carried out, thus obtaining the product. The invention adopts advanced technology, process is simple and feasible, especially three-enzyme composite hydrolysis is adopted, hydrolyzing degree of protein of crassostrea gigas meat can be obviously improved, more amino acid and small peptide can be produced, and product quality is obviously improved. Animal experiment shows that antifatigue effect is good.

The invention belongs to the field of molecular biology research, and relates to a Crassostrea gigas CgIFN-like gene in-vitro recombinant expression technique and function identification thereof. The in-vitro recombinant expression technique is utilized to obtain the Crassostrea gigas interferoid recombinant protein and detects that the Crassostrea gigas CgIFN-like gene in-vitro recombinant protein can activate the immune system of the Crassostrea gigas, cause apoptosis, promote the phagocytic function of the cells for Vibro Splendidus and inhibit the proliferation of the human lung cancer cell A549. The recombinant protein can be used for development of novel immunopotentiators, and development and utilization of marine natural anticancer drugs.

The invention discloses recombinant serum amyloid A capable of enhancing immune response of crassostrea gigas. The recombinant serum amyloid A is characterized that the amino acid sequence is represented by SEQ ID NO. 1. Gene expression of cytokines CgIL17-1, CgIL17-5, and CgTNF in blood lymphocytes can be remarkably promoted inside and outside bodies of the crassostrea gigas, the immune responselevel of the crassostrea gigas can be enhanced, and the recombinant serum amyloid A has application value in preparation of novel immune preparations and other drugs for shellfishes, especially breeding shellfishes.

The invention provides an induction method of a high-heterozygosity tetraploid of a new variety of crassostrea gigas 'Haida No.3'. The method comprises the following steps: taking female triploid crassostrea gigas 'Haida No.3' obtained by inhibiting discharge of first polar bodies of fertilized eggs of diploid crassostrea gigas 'Haida No.3' as a female parent; taking a male diploid 'Haida No.3' as a male parent; after the female parent and the male parent are artificially matured, putting ova into seawater to be matured, and when the ova are round through microscopic examination, the ova are matured; conducting insemination on the cured ova and sperms, starting timing after the sperms and the ova are mixed, treating fertilized ova with cytochalasin B, inhibiting discharging of the first polar bodies of the fertilized ova, and collecting and soaking the fertilized ova; and transferring the fertilized ova into a cultivation container for incubation and larva cultivation. The triploid female parent shellfish is obtained by inhibiting the discharge of the first polar bodies of the fertilized ova of the diploid crassostrea gigas 'Haida No.3', and the tetraploid obtained by the triploid female parent shellfish has higher growth advantage due to higher gene heterozygosity.

The invention provides an SNP locus relevant to rapid growth of crassostrea gigas. The SNP locus is located in an exon of gene LOB, and is a 341 bit starting from the 5' end of the sequence SEQ IDNO:1, and the basic group of the SNP locus is G or T; the GG type shell width of a genotype individual of the mark is remarkably larger than those of TT genotype and TG genotype individuals, the composition of an amino acid sequence is changed, and leucine is changed into phenylalanine. According to application of the SNP locus, the blindness of crassostrea gigas parent screening can be greatly reduced, and crassostrea gigas individuals with high growth speed and genetic stability can be rapidly obtained for breeding or seedling culture.

The invention discloses a method for producing allotetraploid by hybridizing crassostrea angulata diploid and crassostrea hongkongensis triploid. According to the method, the crassostrea hongkongensistriploid is used as a female parent, the full weight is used as a target, the crassostrea angulata diploid is preferably selected as a male parent, and the oyster allotetraploid is produced through drug-induced hybridization. The oyster allotetraploid obtained by the method has the characteristics of euryhalinity and high temperature resistance, is suitable for breeding in low-salt areas such asestuary, and the oyster triploid produced by hybridizing the oyster allotetraploid with the common crassostrea angulata or crassostrea hongkongensis diploid is suitable for aquaculture in sea areas with different salt contents in China , and has wide popularization and application prospects.

The invention discloses a recombinant crassostrea gigas high-mobility family protein r-CgHMGB1, a preparation method and application thereof. The amino acid sequence of the recombinant protein is shown as SEQ ID NO. 1. The preparation method is carried out according to the following steps in sequence: carrying out PCR amplification on a crassostrea gigas CgHMGB1 coding region fragment by using primers P1 and P2, wherein the DNA sequence of the primer P1 is shown as SEQ ID NO. 2, and the DNA sequence of the primer P2 is shown as SEQ ID NO. 3; carrying out BamHI and XholI enzyme digestion on a PCR amplification product and a pET30a vector, then connecting through T4 ligase, and sequencing and identifying a recombinant; transferring the recombinant into an escherichia coli Transetta (DE3) expression strain for induction culture, and then purifying and renaturing. The recombinant crassostrea gigas high-mobility family protein r-CgHMGB1 can be applied to preparation of medicines for resisting vibrio splendidus and escherichia coli.