87 results about "Melanin synthesis" patented technology

The application discloses a whitening and freckle-removing composition, conditioner, medicine and cosmetic. The whitening and freckle-removing composition comprises broccoli, ganoderma lucidum and inula japonica in the composition; the broccoli comprises broccoli raw materials and / or broccoli extract, the ganoderma lucidum comprises ganoderma lucidum raw materials and / or ganoderma lucidum extract, and the inula japonica comprises inula japonica raw materials and / or inula japonica extract. The application has significant synergistic activity by scientific compounding of the broccoli and the inula japonica, has significant inhibitory effect on tyrosinase activity, can inhibit the expression and function of the rate-limiting enzyme TYR in the melanin synthesis process, has significant whitening and freckle-removing activity, is safe and efficient, and can be applied to various conditioners, medicines and cosmetics.

The present invention relates to a functional cosmetic composition comprising plant exosomes derived from ginseng as an active ingredient and a functional cosmetic containing the same. Ginseng exosomes have a bilipid layer structure and are rich in ginsenosides Rb1 and Rg1, which influence skin improvement and whitening. It has been proven that these powerful functional materials, ginsenosides Rb1 and Rg1, are absorbed in large quantities through ginseng exosomes into skin cells, enhance the synthesis of collagen and hyaluronic acid in skin cells, and have the efficacy of inhibiting melanin synthesis. Therefore, the ginseng exosomes rich in ginsenosides Rb1 and Rg1, presented in the present invention, can be beneficially used in functional cosmetic compositions for skin aging prevention, skin whitening, wrinkle improvement and skin cell regeneration.

The invention discloses application of Bombyx mori neuropeptide BommoOKAtype4 in preparation of a medicine for inhibiting melanoma cell growth, and belongs to the technical field of medical biology. Aiming at the technical problems that melanoma is high in malignancy degree, easy to transfer and limited in treatment means, and whether the bombyx mori neuropeptide BommoOKAtype4 can directly inhibit the growth of melanoma cells is not clear yet when the bombyx mori neuropeptide BommoOKAtype4 is mainly used for skin whitening before, the invention provides the application of the bombyx mori neuropeptide in preparing the medicine for inhibiting the melanoma cells. According to the silkworm neuropeptide, gene expression and protein functions of melanin synthesis key enzymes TYR, TRP1 and TRP2 are comprehensively inhibited by down-regulating expression of a core transcription factor MITF, so that melanoma cell proliferation is directly inhibited while melanin synthesis is inhibited, and therefore, the silkworm neuropeptide can be used for preparing drugs for treating melanoma and has a wide application prospect. A novel peptide candidate substance is provided for treatment of melanoma.

The peptides of the present invention exhibit skin whitening activity by inhibiting melanin synthesis in melanocytes and inhibiting melanin body migration. Therefore, the peptide of the present invention can be used as an active ingredient in a medicament for treating or preventing hyperpigmentation diseases caused by excessive deposition of melanin bodies, or as an active ingredient in a cosmetic for skin whitening.

This invention discloses the use of ONO-2952 or a pharmaceutically acceptable salt thereof in the preparation of drugs / cosmetics for treating and / or preventing hyperpigmentation disorders, belonging to the field of pharmaceutical technology. ONO-2952 of this invention inhibits melanin synthesis induced by endogenous factors α-MSH, ACTH, and UVB radiation, thereby inhibiting melanin synthesis by suppressing the expression of key melanin synthesis proteins MITF and Tyrosinase in melanocytes. Therefore, ONO-2952 or a pharmaceutically acceptable salt thereof can be used in the preparation of drugs / cosmetics for treating and / or preventing hyperpigmentation disorders.

This invention relates to the cosmetic use of a composition for improving gray hair. The composition of this invention promotes melanin synthesis in hair by activating the Wnt / β-catenin signaling pathway, thereby exhibiting significant and excellent effects in inhibiting, preventing, improving, alleviating, delaying, or treating gray hair (white hair syndrome).

A whitening composition containing the following components: Mori folium, Panacis quinquefolii radix, (-)-podophyllotoxin, and (+)-glaucarubinone. Further provided is the use of the whitening composition in the preparation of a product for whitening the skin, or a product for inhibiting melanin synthesis, or a product for improving skin pigmentation.

The invention discloses jellyfish polypeptide with tyrosinase inhibitory activity and application of the jellyfish polypeptide, and belongs to the technical field of biology. A jellyfish crude polypeptide is prepared, a jellyfish polypeptide GEPINPEAWLWYYKYVG is identified and optimized from the jellyfish crude polypeptide, and experiments prove that the jellyfish polypeptide provided by the invention can inhibit the activity of tyrosinase in vitro, can inhibit melanin secretion of B16-F10 mouse melanoma cells, and can inhibit the activity of tyrosinase in the cells. Therefore, the jellyfish polypeptide can be used as a tyrosinase inhibitor and an anti-melanin synthesis agent and is applied to the fields of cosmetics, medical treatment and the like. Experiments prove that the jellyfish polypeptide provided by the invention has no cytotoxicity, is derived from ocean, and has no disease risks and religious barriers of terrestrial animals.

The invention relates to the technical field of cosmetics, in particular to a compound active peptide with functions of resisting aging and whitening skin and application of the compound active peptide. Comprising acetyl hexapeptide-1, nonapeptide-1, acetyl tetrapeptide-11, hexapeptide-9 and palmitoyl hexapeptide-12. The compound peptide disclosed by the invention can play a synergistic anti-aging and whitening role on an epidermal layer and a corium layer, and has multiple advantages; the compound peptide acts on epidermal layer keratinocytes, dermal layer fibroblasts and B16F10 cells, and can be used for detecting the senescence of the cells by influencing the expression quantity of cell senescence marker genes, influencing the cell cycle, influencing the expression quantity of extracellular matrix related proteins, influencing melanin synthesis and influencing the secretion quantity of acetylcholine in the cells. Multiple skin anti-aging and whitening effects synergistically exerted on the epidermal layer and the corium layer are achieved.

The application belongs to the technical field of biological materials, and specifically discloses an amino acid whitening polypeptide and a preparation method thereof. The structural general formula of the polypeptide is as follows: R-A-B-NH2, wherein R is a modified acyl group serving as an N terminal and having a carbon number ranging from 6 to 10, A is D-proline and a derivative thereof, and B is D-arginine and a derivative thereof. The amino whitening polypeptide is prepared by using an Fmoc solid-phase synthesis method. The whitening polypeptide has excellent melanin synthesis inhibition activity, and plays a role by targeting inhibition of TRP-1. At a concentration of 1 μM, the melanin synthesis inhibition rate of B16 cells reaches a statistically significant standard, which is significantly better than a conventional whitening agent that can only achieve a similar inhibition effect at a high concentration, and greatly reduces the effective use concentration of a whitening product.

The present application relates to a kind of anti-ultraviolet damage composition and its application in sunscreen product, the anti-ultraviolet damage composition includes the following raw materials by weight parts: 5-8 parts of daphne giraldii leaf extract, 3-7 parts of green algae extract, 1-4 parts of dogwood extract, 8-10 parts of sea buckthorn extract, 2.5-4 parts of avocado butter and 5-8 parts of tea seed oil, each raw material has synergistic effect, by neutralizing the free radical induced by ultraviolet, reduce oxidative stress, protect skin cells from damage;Absorb ultraviolet, reduce the penetration of ultraviolet through skin, thereby reducing the direct damage of ultraviolet to skin;Regulate tyrosinase activity or block the melanin synthesis pathway, reduce the accumulation of melanin, help to prevent and improve the efficacy of anti-ultraviolet damage such as pigmentation, dull and other ways.

The invention relates to the technical field of biology. The invention discloses a strain bacillus sp. JX-7, and the preservation number of the strain bacillus sp. JX-7 is CGMCC (China General Microbiological Culture Collection Center) No.27978. The preservation date of the strain is July 24, 2023, the preservation unit is China General Microbiological Culture Collection Center, the China General Microbiological Culture Collection Center is called CGMCC for short, and the address of the China General Microbiological Culture Collection Center is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing. The bacillus sp. JX-7 provided by the invention can be used for effectively increasing the content of organic carbon in soil and has efficient melanin synthesis capability.

The application belongs to the technical field of biological medicine, and specifically discloses an engineered bacterium for in-situ synthesis of melanin for tumors and application thereof. The engineered bacterium is obtained by transforming a temperature-controlled expression vector containing a gene encoding tyrosinase into a bacterium. The bacterium can accumulate and proliferate in a tumor microenvironment after entering a living organism. The engineered bacterium can realize controllable synthesis of melanin in-situ for tumors. The engineered bacterium constructed in the application can be used for mass production of melanin. After entering a living organism, the bacterium can target and colonize in a tumor microenvironment. Then, melanin synthesis can be started by near-infrared laser irradiation, controllable amplification of drugs at a tumor site and photothermal combined immunotherapy can be realized, and the killing effect and safety on tumors can be improved.

The invention discloses a natural spray for promoting blackening of white hair and growth of hair and a preparation method of the natural spray. The spray is prepared by compounding natural plant extracts of polygonum multiflorum, cacumen biotae, black sesame, fructus viticis, angelica sinensis, ginger, ligusticum wallichii, liquorice and the like, and clove or / and cinnamon extract is creatively adopted as a natural preservative flavor enhancer. Through a multi-target synergistic system of nourishing, blood activating and infiltration promoting and activating, anti-inflammation and stability maintaining, hair follicle melanocytes can be nourished from the source, white hair can be naturally turned into black, dormant hair follicles can be effectively activated, and hair growth is promoted. Preferably, ginseng and lucid ganoderma extracts can also be added to enhance the nourishing and stability maintaining effects. The invention aims to overcome the double defects in the prior art, and provides a multifunctional spray which is 100% purely natural and does not contain any chemically synthesized dye, oxidant, hormone, chemical preservative and harmful additive. The invention aims to realize natural blackening of white hair by gradually recovering melanin synthesis of hair follicles through exogenous nutrition supplement, and effectively promote hair growth and prevent hair loss by stimulating scalp microcirculation, resisting inflammation, relieving and directly activating hair papilla cells. The invention finally provides a daily care product which integrates blackening, hair growth and maintenance, is convenient to use (washing-free after spraying) and does not need to be cleaned. All the components are natural, safe and non-irritant, and the shampoo adopts a washing-free spray design, is convenient to use and is suitable for all hair qualities.

The application discloses a mouse vitiligo induction model based on chronic stress factors, wherein different kinds of stress factors capable of triggering stress response of animals are applied to stimulate the mouse periodically within a certain time period, so as to prevent the mouse from producing habitual response to the stress source and to promote the mouse to become white and the tail epidermis to be flaky. The technical method is different from the existing vitiligo model method, is based on the mechanism of occurrence and development of vitiligo observed in the clinic, and promotes the mouse to truly develop the vitiligo phenomenon from the whole instead of simulating the disease evolution locally. Compared with the existing vitiligo promoting mechanism, the effect is more stable and the authenticity is higher. The technical method is based on the objective fact that the clinical vitiligo patients often combine anxiety and depression to design the stress factors, and considers that the stress factors and the occurrence and development of vitiligo may be inter-caused and viciously circulate, namely, the adverse life events as the stress factors cause the damage and dysfunction of the melanin synthesis system at the micro level and form the vitiligo change at the macro level.

Provided is a composition comprising 20-40 parts by weight of cordyceps militaris powder, 3-25 parts by weight of theaflavins, and 1.25-4.2 parts by weight of astaxanthin, which is a whitening composition or a melanin synthesis signal antagonizing composition. The composition can be used as a main ingredient in whitening food or health food.

This invention relates to the field of probiotic screening and application technology, specifically to a Bifidobacterium adolescentis HC2916 that inhibits melanin synthesis, its microbial preparations, and applications. Bifidobacterium adolescentis HC2916 was deposited on May 16, 2023, at the China General Microbiological Culture Collection Center (CGMCC), with accession number CGMCC No. 27352. This strain exhibits excellent antioxidant capacity, capable of scavenging various free radicals and inhibiting lipid peroxidation. Furthermore, its fermentation supernatant and lysate significantly downregulate the protein and mRNA expression of MITF, a core factor in melanin production signaling, and inhibit the expression of its downstream key enzymes TYRP1 and TYRP2. This effectively suppresses UVA-induced increases in melanin content in skin keratinocytes, demonstrating promising application prospects in the field of skin whitening.

We disclose a composition, comprising a compound, comprising a melanin precursor that spontaneously synthesizes a melanin when in free form in vivo, and a peptide directly covalently bonded to or indirectly linked to the melanin precursor, wherein the direct covalent bond or indirect link of the peptide to the melanin precursor blocks spontaneous synthesis of the melanin in vivo in the absence of protease activity. We also disclose methods of tumor imaging, tumor detection, diagnosis, and treatment, including methods comprising administering, to a patient suffering from a tumor, the composition; and either surgically resecting the tumor or thermally ablating the tumor, wherein the surgical resection is guided at least in part by contrast imparted by, or the thermally ablating targets cells containing, the melanin, wherein the melanin is spontaneously synthesized in the tumor after administering the composition.

The invention relates to the technical field of animal breeding, in particular to an expression vector based on a skin specific promoter and application of the expression vector in preparation of black skin pigs. The expression vector comprises an expression cassette, and the expression cassette comprises a 5'end regulation region of a pig source K5 gene, an intron of a beta-globin gene, a target gene and a 3 'end regulation region of a K14 gene. An expression vector for specifically expressing a target gene in animal skin is provided by adopting a regulatory region of a porcine K5 gene and an intron of a beta-globin gene, and the target gene (such as a melanin synthesis regulatory gene) is integrated by adopting the expression vector and can be efficiently expressed in a porcine somatic cell so as to prepare a pig with specific characters; the method has an important application value in the pig raising industry.

The invention discloses a traditional Chinese medicine composition for nourishing and blacking hair and a preparation method thereof, and belongs to the technical field of daily chemical products, the traditional Chinese medicine composition is composed of fleece-flower root, prepared rehmannia root, Chinese angelica, black sesame, mulberry, glossy privet fruit, eclipta, cacumen biotae, wolfberry, dodder, dried rehmannia root, red sage root, frosted mulberry leaf and sharpleaf galangal fruit. The preparation method comprises the steps of raw material pretreatment, normal-temperature liquor soaking, soft fire concentration, canning and sealing. The traditional Chinese medicine components in the traditional Chinese medicine composition are pharmacologically complementary, melanin synthesis can be promoted, hair follicles can be activated, scalp microcirculation can be improved, and the effects of nourishing and blacking hair, preventing hair loss and growing hair can be achieved. A skin irritation test and a clinical test prove that the composition has no skin irritation and sensitization, is high in external use safety, and can significantly reduce the amount of alopecia after being used for 12 weeks.

The invention provides a composition with whitening, tightening and dull yellow improving effects and a preparation method thereof, the composition comprises raw materials such as nicotinamide, tranexamic acid, glycyrrhiza glabra root extract, biological tightening peptide and the like, and auxiliary components such as grape seed extract, modified hyaluronic acid and the like can also be added. The biological tightening peptide is prepared from palmitoyl tripeptide-5, palmitoyl tetrapeptide-7 and acetyl hexapeptide-8 according to a specific ratio. The preparation method comprises the steps of water phase preparation, active component dispersion, bioactive component addition, homogeneous stabilization and the like. Experiments show that the composition can efficiently inhibit tyrosinase activity and melanin synthesis and promote generation of elastin and collagen, is high in oxidation resistance and good in transdermal absorption effect, can be used for preparing cosmetics and has a good application prospect.

The invention discloses an aspergillus niger endogenous strong promoter Plac and an application thereof in production of catalase, an endogenous lactamase gene promoter Plac with higher expression activity than a currently common glyceraldehyde-3-phosphate dehydrogenase gene promoter is successfully identified in aspergillus niger, and the nucleotide sequence of the endogenous lactamase gene promoter Plac is shown as SEQ ID NO.1; a recombinant expression cassette is constructed by using Plac and catalase and is integrated to an albA site of an aspergillus niger melanin synthesis gene, and the obtained aspergillus niger strain is applied to industrial production of catalase, so that the yield and the enzyme activity of the catalase are remarkably improved.

The application of a drug or medical aesthetic product containing tolvaptan as an active ingredient for inhibiting melanin synthesis. Cell activity detection and melanin content determination show that tolvaptan can significantly reduce the intracellular melanin content without affecting the cell activity. Tyrosinase activity detection shows that tolvaptan does not affect the catalytic activity of TYR. Western blotting experiment shows that tolvaptan can down-regulate the expression levels of TYR family proteins, microphthalmia-associated transcription factor (MITF), cyclic adenosine monophosphate response element binding protein (CREB) and melanocortin 1 receptor (MC1R).

The application provides a composition containing salicin, a product and application thereof, and relates to the technical field of cosmetics. Specifically, the application provides application of salicin in promoting intracellular melanin synthesis, and it is first proved that salicin can promote intracellular melanin synthesis by promoting tyrosinase activity, thereby providing a new functional ingredient for enhancing intracellular melanin synthesis, and preparing a product for improving and treating white hair and vitiligo.

This invention discloses a naturally derived anti-aging moisturizing lotion and its preparation method, relating to the cosmetics field. The anti-aging moisturizing lotion comprises phase A, phase B, and phase C; phase A includes a natural oil-in-water emulsifier, dioctyl carbonate, plant-derived squalane, emollients, coconut oil-caprylate / caprylate, behenyl alcohol, sorbitan caprylate, and Bidens trifoliata extract; phase B includes a solvent, glycerin, 1,3-propanediol, and a thickener; phase C includes sodium hyaluronate, 1,2-pentanediol, Globe amaranth extract, sea fennel callus culture filtrate, and a skin conditioning agent. The anti-aging moisturizing lotion of this application does not cause skin irritation and is suitable for sensitive skin; it also has excellent water retention properties, improving skin elasticity, reducing fine lines or wrinkles, and effectively reducing and decomposing melanin synthesis in the skin, thus achieving true anti-aging effects.

The invention provides new application of an ATOX1 gene and ATOX1 protein, and belongs to the technical field of treatment of vitiligo diseases. The ATOX1 gene serving as a key gene of copper metabolism is disclosed for the first time, and melanin synthesis is promoted by activating the melanocyte copper metabolism process, so that the effect of resisting leucoderma is achieved. The ATOX1 gene and / or the ATOX1 protein can be used as a key biomarker in the vitiligo morbidity process, and a new target and a new molecular basis are provided for vitiligo research.

This invention discloses the establishment and application of a method for melanin synthesis using *Escherichia coli*, belonging to the fields of genetic engineering, enzyme engineering, and biocatalysis. The invention involves the artificial synthesis of a target gene, the sequence of which is shown in SEQ ID NO:1. Based on this gene sequence, a recombinant vector pCDFDuet-1-melC is constructed and transformed into *E. coli* BL21(DE3) to obtain a recombinant genetically engineered bacterium. A whole-cell catalytic system is then constructed based on this recombinant genetically engineered bacterium for melanin synthesis, achieving a melanin yield of 7.46 g / L. This recombinant genetically engineered bacterium possesses advantages such as high catalytic activity and easy heterologous expression, providing a new pathway for the industrial-scale green production of melanin. The enzyme prepared from this recombinant genetically engineered bacterium exhibits the characteristics of tyrosinase and requires low concentrations of Cu. 2+ It can significantly activate enzyme activity.

This invention belongs to the field of cosmetic technology, specifically relating to compositions with skin-whitening and brightening functions and their applications. The skin-whitening and brightening composition of this invention includes: white truffle extract, black truffle extract, vitamin C derivative, melanin transport inhibitor, and collagen supplement. This invention combines five ingredients—white truffle extract, black truffle extract, soluble collagen, vitamin C ethyl ether, and niacinamide—to comprehensively improve the excessive melanin synthesis, inflammatory response, and collagen degradation processes caused by UV radiation through multiple pathways, resulting in a composition with skin-whitening and brightening functions.

The present application relates to the technical field of biology, and discloses a Stenotrophomonas maltophilia JGDQ29-2, which has a preservation number of CGMCC No. 27979.The preservation date of the strain is July 24, 2023, and the preservation unit is the General Microbiological Center of China Microbial Culture Collection Management Committee (CGMCC for short), which is located at No. 3, Beichen West Road, Haidian District, Beijing.The Stenotrophomonas maltophilia JGDQ29-2 provided by the present application can effectively increase the organic carbon content in soil and has high melanin synthesis capacity.

The invention discloses a hair-blacking plant extract composition liquid medicine and a preparation method thereof.The liquid medicine is composed of black beans, sesame, dried apricots, oat, kale, palmarosa, a ligusticum wallichii extracting solution, a mulberry extracting solution and auxiliaries, and tyrosine substrates, trace elements and vitamin B family sources are provided to synergistically promote melanin synthesis; dOPC lipid, glycerol and propylene glycol are combined to form a multi-stage permeation system to improve the scalp absorption rate, active ingredients are reserved by adopting mild processes such as vinegar boiling, steaming extraction, cold pressing and wine soaking, alcohol keeps balanced concentration in a finished product so as to take the effects of sterilization, permeation promotion and moisturizing into account, the pH value of the finally prepared liquid medicine is 4.5-6.5, the ethanol content is not more than 8%, and the prepared liquid medicine can be applied to scalp treatment. The shampoo can effectively improve stress and nutritional white hair, enhance hair follicle activity and scalp nutrition circulation, and realize dual effects of melanogenesis recovery and natural blackening and brightening of hair.