120 results about "Melanin synthesis" patented technology

The invention discloses a multi-effect repair type collagen and application thereof, and belongs to the technical field of synthetic biology. Transdermal peptide TD-1, a transmembrane region of human XVII type collagen, an extracellular sixteenth non-collagen region, an extracellular fifteenth collagen region and a coding gene of a His tag are selected to be connected in series, and a gene sequence is optimized through pichia pastoris codon selection preference; and then the T-COL17R3 is constructed and expressed to obtain the T-COL17R3. Efficacy experiments prove that compared with similar products sold in the market, T-COL17R3 not only has excellent transdermal performance, but also has better cell proliferation and migration promoting capability, better capability of resisting early saccharification product ketoamine and later saccharification product dicarbonyl compounds, better capability of scavenging free radicals and resisting elastase, and can be used for preparing the anti-epidermal drug. In addition, synthesis of melanin in B16-F10 cells can be reduced, so that T-COL17R3 has excellent application potential in development of pharmaceutical compositions or skin care products with repairing, anti-wrinkle and firming or whitening and brightening effects.

The invention discloses a high-transdermal recombinant humanized XVII type collagen as well as a preparation method and application thereof, and belongs to the technical field of synthetic biology. According to the preparation method, transdermal peptide TD-1, different functional fragments of human XVII type collagen and coding genes of His tags are selected to be connected in series, gene sequences are optimized through pichia pastoris codon selection preference firstly, and then the high-transdermal recombinant humanized XVII type collagen is obtained; and then the recombinant humanized XVII type collagen T-COL17-NC16 and the recombinant humanized XVII type collagen T-COL17 are constructed by expression of the recombinant humanized XVII type collagen T-COL17. Effect experiments prove that compared with similar products sold in the market, the skin-care product has more excellent transdermal performance, can improve cell proliferation and migration ability, also has obvious ability of resisting early saccharification product ketoamine and late saccharification product dicarbonyl compounds, better ability of scavenging free radicals and resisting elastase, and can be used for preparing the skin-care product. Moreover, synthesis of melanin in B16-F10 cells can be reduced, and the compound is expected to become a novel functional biological raw material to be applied to the fields of pharmaceutical compositions or skin care products and the like.

The invention relates to the field of cosmetics, in particular to sea rice fermentation filtrate, a preparation method thereof and a whitening cosmetic. Inoculating the first fermentation seed solution and the second fermentation seed solution into a fermentation culture medium, fermenting at 25-30 DEG C to obtain a fermentation product, and removing thalli in the fermentation product; the fermentation culture medium is prepared from the following components in percentage by mass: 5 to 10 percent of sea rice powder, 1 to 5 percent of saccharides and the balance of water; the pH value of the fermentation culture medium is 4-5; the first fermentation seed solution is a saccharomyces cerevisiae strain fermentation seed solution; the second fermentation seed solution is a lactobacillus plantarum strain fermentation seed solution. The method can promote the growth of saccharomyces cerevisiae and lactobacillus plantarum while promoting the release of an active component phytic acid in the sea rice, and has a remarkable effect of removing excessive free radicals and inhibiting the activity of related enzymes for synthesizing melanin; therefore, the whitening effect of the cosmetic can be effectively improved.

The application discloses a whitening and freckle-removing composition, conditioner, medicine and cosmetic. The whitening and freckle-removing composition comprises broccoli, ganoderma lucidum and inula japonica in the composition; the broccoli comprises broccoli raw materials and / or broccoli extract, the ganoderma lucidum comprises ganoderma lucidum raw materials and / or ganoderma lucidum extract, and the inula japonica comprises inula japonica raw materials and / or inula japonica extract. The application has significant synergistic activity by scientific compounding of the broccoli and the inula japonica, has significant inhibitory effect on tyrosinase activity, can inhibit the expression and function of the rate-limiting enzyme TYR in the melanin synthesis process, has significant whitening and freckle-removing activity, is safe and efficient, and can be applied to various conditioners, medicines and cosmetics.

The invention relates to an anti-ultraviolet-damage composition and application thereof in sunscreen products, and the anti-ultraviolet-damage composition is prepared from the following raw materials in parts by weight: 5-8 parts of oxhide tea leaf extract, 3-7 parts of green alga extract, 1-4 parts of dogwood extract, 8-10 parts of sea-buckthorn extract, 2.5-4 parts of butyrospermum parkii butter and 5-8 parts of camellia oleosa seed oil. All the raw materials have a synergistic effect, and free radicals generated by ultraviolet induction are neutralized, so that oxidative stress is relieved, and skin cells are protected from being damaged; ultraviolet rays are absorbed and are prevented from penetrating through the skin, so that direct damage of the ultraviolet rays to the skin is reduced; the activity of tyrosinase is regulated or a melanin synthesis pathway is blocked, the accumulation of melanin is reduced, and the effects of preventing and improving stains, darkness and the like are achieved.

The present invention relates to a functional cosmetic composition comprising plant exosomes derived from ginseng as an active ingredient and a functional cosmetic containing the same. Ginseng exosomes have a bilipid layer structure and are rich in ginsenosides Rb1 and Rg1, which influence skin improvement and whitening. It has been proven that these powerful functional materials, ginsenosides Rb1 and Rg1, are absorbed in large quantities through ginseng exosomes into skin cells, enhance the synthesis of collagen and hyaluronic acid in skin cells, and have the efficacy of inhibiting melanin synthesis. Therefore, the ginseng exosomes rich in ginsenosides Rb1 and Rg1, presented in the present invention, can be beneficially used in functional cosmetic compositions for skin aging prevention, skin whitening, wrinkle improvement and skin cell regeneration.

The invention discloses a whitening and freckle-removing composition, a conditioner, a medicine and a cosmetic. The whitening and freckle-removing composition is prepared from emblic leafflower fruit, passion fruit and Chinese ladiestresses root and leaf A; the phyllanthus emblica comprises a phyllanthus emblica raw material and / or a phyllanthus emblica extract, and the passion fruit comprises a passion fruit raw material and / or a passion fruit extract. Through scientific compatibility of the Chinese ladiestresses root-ginseng A and the second active component comprising one or more of the emblic leafflower fruit, the passion fruit and the salvia officinalis, the Chinese ladiestresses root-ginseng A has a remarkable inhibition effect on tyrosinase activity, can inhibit expression and functions of rate-limiting enzyme TYR in the melanin synthesis process, has a remarkable whitening and freckle-removing active effect, and has a remarkable whitening and freckle-removing effect. The composition is safe and efficient, and can be applied to various conditioners, medicines and cosmetics.

The invention discloses application of Bombyx mori neuropeptide BommoOKAtype4 in preparation of a medicine for inhibiting melanoma cell growth, and belongs to the technical field of medical biology. Aiming at the technical problems that melanoma is high in malignancy degree, easy to transfer and limited in treatment means, and whether the bombyx mori neuropeptide BommoOKAtype4 can directly inhibit the growth of melanoma cells is not clear yet when the bombyx mori neuropeptide BommoOKAtype4 is mainly used for skin whitening before, the invention provides the application of the bombyx mori neuropeptide in preparing the medicine for inhibiting the melanoma cells. According to the silkworm neuropeptide, gene expression and protein functions of melanin synthesis key enzymes TYR, TRP1 and TRP2 are comprehensively inhibited by down-regulating expression of a core transcription factor MITF, so that melanoma cell proliferation is directly inhibited while melanin synthesis is inhibited, and therefore, the silkworm neuropeptide can be used for preparing drugs for treating melanoma and has a wide application prospect. A novel peptide candidate substance is provided for treatment of melanoma.

The peptides of the present invention exhibit skin whitening activity by inhibiting melanin synthesis in melanocytes and inhibiting melanin body migration. Therefore, the peptide of the present invention can be used as an active ingredient in a medicament for treating or preventing hyperpigmentation diseases caused by excessive deposition of melanin bodies, or as an active ingredient in a cosmetic for skin whitening.

This invention discloses the use of ONO-2952 or a pharmaceutically acceptable salt thereof in the preparation of drugs / cosmetics for treating and / or preventing hyperpigmentation disorders, belonging to the field of pharmaceutical technology. ONO-2952 of this invention inhibits melanin synthesis induced by endogenous factors α-MSH, ACTH, and UVB radiation, thereby inhibiting melanin synthesis by suppressing the expression of key melanin synthesis proteins MITF and Tyrosinase in melanocytes. Therefore, ONO-2952 or a pharmaceutically acceptable salt thereof can be used in the preparation of drugs / cosmetics for treating and / or preventing hyperpigmentation disorders.

This invention relates to the cosmetic use of a composition for improving gray hair. The composition of this invention promotes melanin synthesis in hair by activating the Wnt / β-catenin signaling pathway, thereby exhibiting significant and excellent effects in inhibiting, preventing, improving, alleviating, delaying, or treating gray hair (white hair syndrome).

A whitening composition containing the following components: Mori folium, Panacis quinquefolii radix, (-)-podophyllotoxin, and (+)-glaucarubinone. Further provided is the use of the whitening composition in the preparation of a product for whitening the skin, or a product for inhibiting melanin synthesis, or a product for improving skin pigmentation.

The invention discloses jellyfish polypeptide with tyrosinase inhibitory activity and application of the jellyfish polypeptide, and belongs to the technical field of biology. A jellyfish crude polypeptide is prepared, a jellyfish polypeptide GEPINPEAWLWYYKYVG is identified and optimized from the jellyfish crude polypeptide, and experiments prove that the jellyfish polypeptide provided by the invention can inhibit the activity of tyrosinase in vitro, can inhibit melanin secretion of B16-F10 mouse melanoma cells, and can inhibit the activity of tyrosinase in the cells. Therefore, the jellyfish polypeptide can be used as a tyrosinase inhibitor and an anti-melanin synthesis agent and is applied to the fields of cosmetics, medical treatment and the like. Experiments prove that the jellyfish polypeptide provided by the invention has no cytotoxicity, is derived from ocean, and has no disease risks and religious barriers of terrestrial animals.

The invention relates to the technical field of cosmetics, in particular to a compound active peptide with functions of resisting aging and whitening skin and application of the compound active peptide. Comprising acetyl hexapeptide-1, nonapeptide-1, acetyl tetrapeptide-11, hexapeptide-9 and palmitoyl hexapeptide-12. The compound peptide disclosed by the invention can play a synergistic anti-aging and whitening role on an epidermal layer and a corium layer, and has multiple advantages; the compound peptide acts on epidermal layer keratinocytes, dermal layer fibroblasts and B16F10 cells, and can be used for detecting the senescence of the cells by influencing the expression quantity of cell senescence marker genes, influencing the cell cycle, influencing the expression quantity of extracellular matrix related proteins, influencing melanin synthesis and influencing the secretion quantity of acetylcholine in the cells. Multiple skin anti-aging and whitening effects synergistically exerted on the epidermal layer and the corium layer are achieved.

The invention discloses application of rifudine in preparation of a preparation for promoting cell melanin synthesis, and belongs to the technical field of special drugs. The invention provides a new application of rifudine, which can promote synthesis and secretion of melanin and expression of TYR, TRP-1 and TRP-2, provides a new functional component for enhancing synthesis of melanin in vivo and preparing products for treating vitiligo and the like, and has a wide application prospect in the fields of pharmaceutical preparations, cosmetics, care products, beauty products and the like.

The application belongs to the technical field of biological materials, and specifically discloses an amino acid whitening polypeptide and a preparation method thereof. The structural general formula of the polypeptide is as follows: R-A-B-NH2, wherein R is a modified acyl group serving as an N terminal and having a carbon number ranging from 6 to 10, A is D-proline and a derivative thereof, and B is D-arginine and a derivative thereof. The amino whitening polypeptide is prepared by using an Fmoc solid-phase synthesis method. The whitening polypeptide has excellent melanin synthesis inhibition activity, and plays a role by targeting inhibition of TRP-1. At a concentration of 1 μM, the melanin synthesis inhibition rate of B16 cells reaches a statistically significant standard, which is significantly better than a conventional whitening agent that can only achieve a similar inhibition effect at a high concentration, and greatly reduces the effective use concentration of a whitening product.

The invention relates to the technical field of skin care products, in particular to a turnip extracting solution and a preparation method and application thereof. The preparation method comprises the following steps: mixing turnip with water, decocting to obtain a decoction, decolorizing, filtering and concentrating to obtain the turnip extracting solution. The turnip extracting solution is used for effectively inhibiting B16 cells from generating melanin and inhibiting the activity of tyrosinase in a melanin synthesis path; the increase of the active oxygen level in HaCaT cells is effectively prevented, and the antioxidant effect is obvious; the inflammation of the SDS surfactant on HaCaT cells is effectively prevented, and the generation of an inflammatory factor TNF-alpha is reduced; the expression of cell natural moisturizing factors, ceramide de novo synthesis rate-limiting enzymes and epidermal barrier related genes is up-regulated; the composition can effectively promote the generation of cell collagen and elastin, reduces the synthesis of matrix metalloproteinase 1 for degrading collagen under ultraviolet radiation, and has an anti-aging effect.

The present application relates to a kind of anti-ultraviolet damage composition and its application in sunscreen product, the anti-ultraviolet damage composition includes the following raw materials by weight parts: 5-8 parts of daphne giraldii leaf extract, 3-7 parts of green algae extract, 1-4 parts of dogwood extract, 8-10 parts of sea buckthorn extract, 2.5-4 parts of avocado butter and 5-8 parts of tea seed oil, each raw material has synergistic effect, by neutralizing the free radical induced by ultraviolet, reduce oxidative stress, protect skin cells from damage;Absorb ultraviolet, reduce the penetration of ultraviolet through skin, thereby reducing the direct damage of ultraviolet to skin;Regulate tyrosinase activity or block the melanin synthesis pathway, reduce the accumulation of melanin, help to prevent and improve the efficacy of anti-ultraviolet damage such as pigmentation, dull and other ways.

The invention relates to the technical field of biology. The invention discloses a strain bacillus sp. JX-7, and the preservation number of the strain bacillus sp. JX-7 is CGMCC (China General Microbiological Culture Collection Center) No.27978. The preservation date of the strain is July 24, 2023, the preservation unit is China General Microbiological Culture Collection Center, the China General Microbiological Culture Collection Center is called CGMCC for short, and the address of the China General Microbiological Culture Collection Center is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing. The bacillus sp. JX-7 provided by the invention can be used for effectively increasing the content of organic carbon in soil and has efficient melanin synthesis capability.

The application belongs to the technical field of biological medicine, and specifically discloses an engineered bacterium for in-situ synthesis of melanin for tumors and application thereof. The engineered bacterium is obtained by transforming a temperature-controlled expression vector containing a gene encoding tyrosinase into a bacterium. The bacterium can accumulate and proliferate in a tumor microenvironment after entering a living organism. The engineered bacterium can realize controllable synthesis of melanin in-situ for tumors. The engineered bacterium constructed in the application can be used for mass production of melanin. After entering a living organism, the bacterium can target and colonize in a tumor microenvironment. Then, melanin synthesis can be started by near-infrared laser irradiation, controllable amplification of drugs at a tumor site and photothermal combined immunotherapy can be realized, and the killing effect and safety on tumors can be improved.

The invention discloses a natural spray for promoting blackening of white hair and growth of hair and a preparation method of the natural spray. The spray is prepared by compounding natural plant extracts of polygonum multiflorum, cacumen biotae, black sesame, fructus viticis, angelica sinensis, ginger, ligusticum wallichii, liquorice and the like, and clove or / and cinnamon extract is creatively adopted as a natural preservative flavor enhancer. Through a multi-target synergistic system of nourishing, blood activating and infiltration promoting and activating, anti-inflammation and stability maintaining, hair follicle melanocytes can be nourished from the source, white hair can be naturally turned into black, dormant hair follicles can be effectively activated, and hair growth is promoted. Preferably, ginseng and lucid ganoderma extracts can also be added to enhance the nourishing and stability maintaining effects. The invention aims to overcome the double defects in the prior art, and provides a multifunctional spray which is 100% purely natural and does not contain any chemically synthesized dye, oxidant, hormone, chemical preservative and harmful additive. The invention aims to realize natural blackening of white hair by gradually recovering melanin synthesis of hair follicles through exogenous nutrition supplement, and effectively promote hair growth and prevent hair loss by stimulating scalp microcirculation, resisting inflammation, relieving and directly activating hair papilla cells. The invention finally provides a daily care product which integrates blackening, hair growth and maintenance, is convenient to use (washing-free after spraying) and does not need to be cleaned. All the components are natural, safe and non-irritant, and the shampoo adopts a washing-free spray design, is convenient to use and is suitable for all hair qualities.

The application discloses a mouse vitiligo induction model based on chronic stress factors, wherein different kinds of stress factors capable of triggering stress response of animals are applied to stimulate the mouse periodically within a certain time period, so as to prevent the mouse from producing habitual response to the stress source and to promote the mouse to become white and the tail epidermis to be flaky. The technical method is different from the existing vitiligo model method, is based on the mechanism of occurrence and development of vitiligo observed in the clinic, and promotes the mouse to truly develop the vitiligo phenomenon from the whole instead of simulating the disease evolution locally. Compared with the existing vitiligo promoting mechanism, the effect is more stable and the authenticity is higher. The technical method is based on the objective fact that the clinical vitiligo patients often combine anxiety and depression to design the stress factors, and considers that the stress factors and the occurrence and development of vitiligo may be inter-caused and viciously circulate, namely, the adverse life events as the stress factors cause the damage and dysfunction of the melanin synthesis system at the micro level and form the vitiligo change at the macro level.

Provided is a composition comprising 20-40 parts by weight of cordyceps militaris powder, 3-25 parts by weight of theaflavins, and 1.25-4.2 parts by weight of astaxanthin, which is a whitening composition or a melanin synthesis signal antagonizing composition. The composition can be used as a main ingredient in whitening food or health food.

This invention relates to the field of probiotic screening and application technology, specifically to a Bifidobacterium adolescentis HC2916 that inhibits melanin synthesis, its microbial preparations, and applications. Bifidobacterium adolescentis HC2916 was deposited on May 16, 2023, at the China General Microbiological Culture Collection Center (CGMCC), with accession number CGMCC No. 27352. This strain exhibits excellent antioxidant capacity, capable of scavenging various free radicals and inhibiting lipid peroxidation. Furthermore, its fermentation supernatant and lysate significantly downregulate the protein and mRNA expression of MITF, a core factor in melanin production signaling, and inhibit the expression of its downstream key enzymes TYRP1 and TYRP2. This effectively suppresses UVA-induced increases in melanin content in skin keratinocytes, demonstrating promising application prospects in the field of skin whitening.

We disclose a composition, comprising a compound, comprising a melanin precursor that spontaneously synthesizes a melanin when in free form in vivo, and a peptide directly covalently bonded to or indirectly linked to the melanin precursor, wherein the direct covalent bond or indirect link of the peptide to the melanin precursor blocks spontaneous synthesis of the melanin in vivo in the absence of protease activity. We also disclose methods of tumor imaging, tumor detection, diagnosis, and treatment, including methods comprising administering, to a patient suffering from a tumor, the composition; and either surgically resecting the tumor or thermally ablating the tumor, wherein the surgical resection is guided at least in part by contrast imparted by, or the thermally ablating targets cells containing, the melanin, wherein the melanin is spontaneously synthesized in the tumor after administering the composition.

The invention relates to the technical field of animal breeding, in particular to an expression vector based on a skin specific promoter and application of the expression vector in preparation of black skin pigs. The expression vector comprises an expression cassette, and the expression cassette comprises a 5'end regulation region of a pig source K5 gene, an intron of a beta-globin gene, a target gene and a 3 'end regulation region of a K14 gene. An expression vector for specifically expressing a target gene in animal skin is provided by adopting a regulatory region of a porcine K5 gene and an intron of a beta-globin gene, and the target gene (such as a melanin synthesis regulatory gene) is integrated by adopting the expression vector and can be efficiently expressed in a porcine somatic cell so as to prepare a pig with specific characters; the method has an important application value in the pig raising industry.

The invention discloses a traditional Chinese medicine composition for nourishing and blacking hair and a preparation method thereof, and belongs to the technical field of daily chemical products, the traditional Chinese medicine composition is composed of fleece-flower root, prepared rehmannia root, Chinese angelica, black sesame, mulberry, glossy privet fruit, eclipta, cacumen biotae, wolfberry, dodder, dried rehmannia root, red sage root, frosted mulberry leaf and sharpleaf galangal fruit. The preparation method comprises the steps of raw material pretreatment, normal-temperature liquor soaking, soft fire concentration, canning and sealing. The traditional Chinese medicine components in the traditional Chinese medicine composition are pharmacologically complementary, melanin synthesis can be promoted, hair follicles can be activated, scalp microcirculation can be improved, and the effects of nourishing and blacking hair, preventing hair loss and growing hair can be achieved. A skin irritation test and a clinical test prove that the composition has no skin irritation and sensitization, is high in external use safety, and can significantly reduce the amount of alopecia after being used for 12 weeks.

The invention provides a composition with whitening, tightening and dull yellow improving effects and a preparation method thereof, the composition comprises raw materials such as nicotinamide, tranexamic acid, glycyrrhiza glabra root extract, biological tightening peptide and the like, and auxiliary components such as grape seed extract, modified hyaluronic acid and the like can also be added. The biological tightening peptide is prepared from palmitoyl tripeptide-5, palmitoyl tetrapeptide-7 and acetyl hexapeptide-8 according to a specific ratio. The preparation method comprises the steps of water phase preparation, active component dispersion, bioactive component addition, homogeneous stabilization and the like. Experiments show that the composition can efficiently inhibit tyrosinase activity and melanin synthesis and promote generation of elastin and collagen, is high in oxidation resistance and good in transdermal absorption effect, can be used for preparing cosmetics and has a good application prospect.

The invention discloses an aspergillus niger endogenous strong promoter Plac and an application thereof in production of catalase, an endogenous lactamase gene promoter Plac with higher expression activity than a currently common glyceraldehyde-3-phosphate dehydrogenase gene promoter is successfully identified in aspergillus niger, and the nucleotide sequence of the endogenous lactamase gene promoter Plac is shown as SEQ ID NO.1; a recombinant expression cassette is constructed by using Plac and catalase and is integrated to an albA site of an aspergillus niger melanin synthesis gene, and the obtained aspergillus niger strain is applied to industrial production of catalase, so that the yield and the enzyme activity of the catalase are remarkably improved.

The invention relates to the technical field of daily chemicals, in particular to an anti-aging composition containing DNA sodium and application of the anti-aging composition. The composition is prepared from DNA sodium, hydrolyzed elastin, collagen, snake venom tripeptide, vitamin E and sodium hyaluronate. According to the emulsion disclosed by the invention, various active ingredients such as the DNA sodium, the snake venom tripeptide, the elastin peptide, the low-molecular collagen peptide, the nicotinamide, the alpha-arbutin and the purple blue bletilla striata bulb fermentation liquor are organically combined, so that multiple anti-aging and whitening effects of resisting oxidation, inhibiting melanin synthesis, promoting collagen and elastin generation, improving uneven skin color and the like can be synergistically realized. Purple blue bletilla striata bulbs are subjected to lactobacillus-candida compound fermentation, the product does not contain coarse particles after refined filtration treatment, is clarified, is stable for a long time after being added into an emulsion system, and is not easy to separate out and precipitate or cause system layering. The emulsion is light in structure, not sticky and greasy, endows skin with soft and moist touch feeling, and is suitable for various skin types including sensitive skin, and active ingredients are easy to permeate and absorb.