17 results about "Melanin synthesis" patented technology

This invention relates to the cosmetic use of a composition for improving gray hair. The composition of this invention promotes melanin synthesis in hair by activating the Wnt / β-catenin signaling pathway, thereby exhibiting significant and excellent effects in inhibiting, preventing, improving, alleviating, delaying, or treating gray hair (white hair syndrome).

A whitening composition containing the following components: Mori folium, Panacis quinquefolii radix, (-)-podophyllotoxin, and (+)-glaucarubinone. Further provided is the use of the whitening composition in the preparation of a product for whitening the skin, or a product for inhibiting melanin synthesis, or a product for improving skin pigmentation.

The application belongs to the technical field of biological materials, and specifically discloses an amino acid whitening polypeptide and a preparation method thereof. The structural general formula of the polypeptide is as follows: R-A-B-NH2, wherein R is a modified acyl group serving as an N terminal and having a carbon number ranging from 6 to 10, A is D-proline and a derivative thereof, and B is D-arginine and a derivative thereof. The amino whitening polypeptide is prepared by using an Fmoc solid-phase synthesis method. The whitening polypeptide has excellent melanin synthesis inhibition activity, and plays a role by targeting inhibition of TRP-1. At a concentration of 1 μM, the melanin synthesis inhibition rate of B16 cells reaches a statistically significant standard, which is significantly better than a conventional whitening agent that can only achieve a similar inhibition effect at a high concentration, and greatly reduces the effective use concentration of a whitening product.

The invention relates to the technical field of biology. The invention discloses a strain bacillus sp. JX-7, and the preservation number of the strain bacillus sp. JX-7 is CGMCC (China General Microbiological Culture Collection Center) No.27978. The preservation date of the strain is July 24, 2023, the preservation unit is China General Microbiological Culture Collection Center, the China General Microbiological Culture Collection Center is called CGMCC for short, and the address of the China General Microbiological Culture Collection Center is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing. The bacillus sp. JX-7 provided by the invention can be used for effectively increasing the content of organic carbon in soil and has efficient melanin synthesis capability.

This invention relates to the field of probiotic screening and application technology, specifically to a Bifidobacterium adolescentis HC2916 that inhibits melanin synthesis, its microbial preparations, and applications. Bifidobacterium adolescentis HC2916 was deposited on May 16, 2023, at the China General Microbiological Culture Collection Center (CGMCC), with accession number CGMCC No. 27352. This strain exhibits excellent antioxidant capacity, capable of scavenging various free radicals and inhibiting lipid peroxidation. Furthermore, its fermentation supernatant and lysate significantly downregulate the protein and mRNA expression of MITF, a core factor in melanin production signaling, and inhibit the expression of its downstream key enzymes TYRP1 and TYRP2. This effectively suppresses UVA-induced increases in melanin content in skin keratinocytes, demonstrating promising application prospects in the field of skin whitening.

This invention discloses the establishment and application of a method for melanin synthesis using *Escherichia coli*, belonging to the fields of genetic engineering, enzyme engineering, and biocatalysis. The invention involves the artificial synthesis of a target gene, the sequence of which is shown in SEQ ID NO:1. Based on this gene sequence, a recombinant vector pCDFDuet-1-melC is constructed and transformed into *E. coli* BL21(DE3) to obtain a recombinant genetically engineered bacterium. A whole-cell catalytic system is then constructed based on this recombinant genetically engineered bacterium for melanin synthesis, achieving a melanin yield of 7.46 g / L. This recombinant genetically engineered bacterium possesses advantages such as high catalytic activity and easy heterologous expression, providing a new pathway for the industrial-scale green production of melanin. The enzyme prepared from this recombinant genetically engineered bacterium exhibits the characteristics of tyrosinase and requires low concentrations of Cu. 2+ It can significantly activate enzyme activity.

The present application relates to the technical field of biology, and discloses a Stenotrophomonas maltophilia JGDQ29-2, which has a preservation number of CGMCC No. 27979.The preservation date of the strain is July 24, 2023, and the preservation unit is the General Microbiological Center of China Microbial Culture Collection Management Committee (CGMCC for short), which is located at No. 3, Beichen West Road, Haidian District, Beijing.The Stenotrophomonas maltophilia JGDQ29-2 provided by the present application can effectively increase the organic carbon content in soil and has high melanin synthesis capacity.

This invention belongs to the field of daily chemical products technology, and particularly relates to a hair-darkening composition, its preparation method, and its application. The hair-darkening composition provided by this invention is scientifically formulated with acetyl hexapeptide-1 and extracts of Polygonum multiflorum, Epimedium, and Aristolochia debilis root extract and Avena sativa seed extract. Through the synergistic effect of small molecule peptides and multiple plant active ingredients, it achieves multi-target and multi-pathway regulation of melanin production mechanisms. Each active ingredient, under specific ratios, exhibits significant synergistic effects, synergistically promoting melanin synthesis in hair follicles. Compared to single ingredients, it effectively enhances hair-darkening efficacy, providing a stable and efficient technical solution for improving gray hair and restoring its natural color, possessing outstanding practicality and application value.

This invention discloses a regulatory protein that activates melanin in the dermal papilla to promote hair darkening and its preparation method, belonging to the field of biotechnology. The method includes: identifying key endogenous proteins that positively regulate melanin synthesis in dermal papilla cells through multi-omics analysis; constructing a humanized recombinant regulatory protein fused with a membrane-penetrating peptide; loading its coding sequence into a eukaryotic expression vector to achieve local and sustained expression; verifying its melanin-promoting efficacy using a three-dimensional hair follicle organoid model and animal experiments; and formulating a thermosensitive gel for topical application to improve hair follicle-targeted delivery efficiency. This invention aims to address the problems of unclear mechanisms of action and limited efficacy in existing hair-darkening products. It can directly activate the MITF signaling pathway, significantly promote the expression of key enzymes such as TYR and TRP-1, induce melanosome synthesis and deposition, resulting in a hair color darkening rate of over 95%, with long-lasting efficacy and good safety.

The present application relates to the technical field of polypeptide compounds, and particularly relates to a whitening polypeptide compound, a preparation method and application thereof, wherein the whitening polypeptide compound is obtained by free arrangement and combination of tryptophan, tyrosine and beta-alanine through an amide bond. The whitening polypeptide compound provided by the present application has excellent whitening activity, and is verified by B16-F10 cells induced by alpha-MSH. The whitening polypeptide compound can efficiently inhibit skin melanin synthesis, and has the effects of whitening and freckle lightening. Meanwhile, the whitening polypeptide compound has high whitening activity, good mild safety, a simple preparation process, high synthesis efficiency, low cost, strong stability in a cosmetic system, good skin permeability, can simultaneously realize efficient whitening, freckle lightening and skin barrier repair, can be widely applied to whitening cosmetics to prepare various dosage forms, improve product whitening durability and stability, has high application value, and meets the demand of the cosmetic industry for high-performance mild whitening active ingredients.

This invention relates to a plant composition with whitening effects and its application. The whitening plant composition includes extracts of *Scleroderma wilfordii*, *Myrothamnus flabellifolia*, and *Solanum nigrum* root. The whitening plant composition of this invention utilizes the combination of three plant extracts, resulting in a rational formulation where each active ingredient complements the others, synergistically enhancing their effects. This effectively inhibits tyrosinase activity and reduces melanin synthesis, exhibiting significant whitening efficacy. When applied to cosmetics, this plant composition results in stable products that also provide skin repair, reduce the irritation of whitening ingredients, and are suitable for various skin types, fully meeting consumers' demand for natural, gentle, and highly effective whitening products.

This invention relates to the field of biomedical technology, and particularly to a peony-derived exosome-like nanovesicle, its preparation method, and its applications. The nanovesicles are derived from the flowers, leaves, or buds of *Paeonia suffruticosa*, with a particle size of 30–200 nm, and exhibit a cup-shaped or near-circular membrane vesicle structure. These nanovesicles possess the functions of scavenging reactive oxygen species, inhibiting the expression of cellular aging markers P21 / P53 / γ-H2AX, inhibiting the expression of matrix metalloproteinase MMP2 and inflammatory factors IL-6 / IL-8 / IL-1β / TNF-α, and simultaneously downregulating the MITF / TYR / DCT pathway to inhibit melanin synthesis. These nanovesicles can simultaneously alleviate skin photoaging and inhibit melanin production, and can be used to prepare anti-photoaging, whitening, or dual-effect skincare products, pharmaceuticals, or topical skin preparations.

The application discloses a whitening composition containing controlled-release ascorbic acid and application thereof, and belongs to the technical field of cosmetic preparations. The composition comprises the following components in percentage by weight: Aframomum melegueta seed extract 0.1-5%, sodium mannose phosphate 0.1-5%, controlled-release ascorbic acid 10-20%, crocus sativus flower extract 0.1-5%, dipropylene glycol 15-30% and water in remainder. The application controls and releases ascorbic acid, significantly improves the stability and transdermal persistence of ascorbic acid in the formula, and realizes multi-target synergistic effect on the basis of compounding the controlled-release ascorbic acid with the Aframomum melegueta seed extract, the crocus sativus flower extract and the sodium mannose phosphate. Experiments prove that the composition can not only efficiently inhibit tyrosinase activity and melanin synthesis in B16F10 cells, but also has superiority in long-term stability and long-acting inhibition effect.

The application provides a method for inducing rapid rooting of lily bulbils and application thereof, and the lily bulbil (diameter>1.0cm) is used as material, the outer scale is peeled off after surface disinfection and low-temperature pretreatment, the peeled-off outer scale is placed in sterile sawdust with humidity of 15%-25% and cultured at 26 DEG C in dark, and the lily bulbil can be rooted. Compared with a control, the lily bulbil obtained by the method has developed root system, early root system germination, and the number and length of roots are obviously increased, the low-temperature pretreatment time is short, the culture period is short, and a large amount of lily root system can be rapidly obtained in about 10 weeks, and the lily root system can be applied to preparation of medicines, foods and cosmetics resisting melanin synthesis and inflammation reaction, and the method can also realize rapid propagation of lily and provide a new selection, and has important significance. The material is easy to obtain, the method is non-tissue culture, simple and convenient to operate, and can be produced on a large scale, and has good application prospect.

The present invention relates to a whitening composition comprising a whitening effective amount of a compound of formula (I), or a tautomer, stereoisomer, hydrate, solvate, salt, or polymorph thereof, wherein R 1 、R 2 、R 3 、R 4 、R 5 、R 6 、R 7 、m and n are as defined in the specification and claims. The present invention also relates to a compound of formula (I2) or a tautomer, stereoisomer, hydrate, solvate, salt, or polymorph thereof, wherein R 1 、R 2 、R 3 、R 4 、R 5 、R 6 、and R 7 are as defined in the specification and claims. The present invention also relates to the use of at least one compound of formula (I) or any subgroup thereof, or a mixture thereof, as a depigmenting agent. The present invention also relates to the use of at least one compound of formula (I) or any subgroup thereof, or the use of a whitening composition, for preventing and / or reducing pigmentation of normal skin. The present invention also relates to the use of at least one compound, or the use of a whitening composition according to the first aspect, for reducing the amount of melanin in melanocytes, for reducing melanin synthesis, for impairing melanin transport, and / or for impairing the distribution of melanin in the upper basal layer. JPEG2026520910000038.jpg3191

This invention discloses a small molecule peptide derived from *Hirudo medicinalis*, with the amino acid sequence Leu-Leu-Phe-Arg. The peptide was obtained by enzymatically hydrolyzing the waste protein residue after purifying hirudin from *Hirudo medicinalis*, combined with tyrosinase-functionalized magnetic bead affinity fishing technology and mass spectrometry identification. In vitro kinetics and molecular dynamics simulations confirmed that the peptide LLFR significantly prolongs the lag time of monophenolase reactions. It penetrates the catalytic pocket of tyrosinase through an "induced fit" mechanism, forming a stable dead-end complex with the enzyme and inducing the unfolding of the enzyme's tertiary structure. Cell experiments confirmed that LLFR significantly inhibits tyrosinase activity and melanin synthesis in melanoma cells without significant cytotoxicity. This peptide exhibits high safety and good water solubility, providing a new approach for the high-value utilization of industrial waste from *Hirudo medicinalis*, and is suitable for the industrial production of whitening cosmetics and functional products.

This invention discloses a method for analyzing the anti-aging and skin whitening mechanisms of bergamot extract based on network pharmacology and experimental verification. Belonging to the field of computer-aided drug screening technology, this method uses network pharmacology to predict the core targets of the active ingredients in bergamot and screens the signaling pathways regulated by the bergamot extract. Through DPPH free radical scavenging experiments, mushroom tyrosinase inhibition experiments, B16F10 cell melanin production inhibition experiments, HSF cell type I collagen secretion promotion experiments, and a UVB-induced mouse skin pigmentation model, the method systematically verifies that bergamot extract has multiple effects, including significantly inhibiting melanin synthesis, scavenging free radicals, promoting collagen secretion, and improving skin pigmentation. This invention is the first to combine computational prediction with experimental verification, fully elucidating the whitening and anti-aging potential of bergamot extract and its underlying molecular mechanisms, providing a reliable method and basis for developing novel natural cosmetic raw materials.