Composition capable of resisting ultraviolet damage and application of composition in sunscreen product

Abstract

The invention relates to an anti-ultraviolet-damage composition and application thereof in sunscreen products, and the anti-ultraviolet-damage composition is prepared from the following raw materials in parts by weight: 5-8 parts of oxhide tea leaf extract, 3-7 parts of green alga extract, 1-4 parts of dogwood extract, 8-10 parts of sea-buckthorn extract, 2.5-4 parts of butyrospermum parkii butter and 5-8 parts of camellia oleosa seed oil. All the raw materials have a synergistic effect, and free radicals generated by ultraviolet induction are neutralized, so that oxidative stress is relieved, and skin cells are protected from being damaged; ultraviolet rays are absorbed and are prevented from penetrating through the skin, so that direct damage of the ultraviolet rays to the skin is reduced; the activity of tyrosinase is regulated or a melanin synthesis pathway is blocked, the accumulation of melanin is reduced, and the effects of preventing and improving stains, darkness and the like are achieved.

Description

Technical Field

[0001] The present invention relates to the technical field of daily chemicals, and specifically to a composition for resisting ultraviolet damage and its application in sunscreen products. Background Art

[0002] Ultraviolet rays consist of short-wave UVC, medium-wave UVB, and long-wave UVA. UVA accounts for approximately 4.9% of sunlight energy and can penetrate the dermis, causing skin aging, pigmentation, darkening, and even skin cancer. UVB penetrates the surface layer of the skin, dilating blood vessels and causing localized redness, swelling, and warmth. These are the most common symptoms of sunburn. To mitigate the harmful effects of UV rays on the skin, compositions containing anti-UV damage agents are incorporated into skincare products. These agents form a protective layer on the skin's surface, blocking harmful UV rays and other harmful rays, thereby providing protection. Summary of the Invention

[0003] In view of this, a composition for resisting ultraviolet damage is provided to solve the above problems.

[0004] To achieve the above object, the technical solution adopted by the present invention is:

[0005] A composition for resisting ultraviolet damage comprises the following raw materials, measured by weight: 5-8 parts of cowhide tea leaf extract, 3-7 parts of green algae extract, 1-4 parts of cornus officinalis extract, 8-10 parts of sea buckthorn extract, 2.5-4 parts of avocado tree fat and 5-8 parts of camellia oil.

[0006] Rhododendron chrysanthum Pall. (Rhododendron chrysanthum Pall.) contains active ingredients such as chlorogenic acid, quercetin, and hyperoside. Chlorogenic acid scavenges free radicals and reduces oxidative stress. Quercetin has antioxidant properties, protecting epidermal lipids from UV degradation, thereby strengthening the skin barrier and reducing water loss.

[0007] Green algae extract is rich in various active ingredients, such as carotenoids, polyphenols, and polysaccharides. These active ingredients possess powerful antioxidant properties, scavenging free radicals induced by UV rays and mitigating oxidative damage. They also absorb UV rays, reducing damage to skin cells. They also enhance the skin's moisturizing and barrier function, promoting skin repair.

[0008] Cornus officinalis extract is rich in various active ingredients, such as ursolic acid and flavonoids. These active ingredients have significant antioxidant properties, capable of scavenging excess reactive oxygen species (ROS) induced by UV rays, reducing oxidative stress, and inhibiting the production of UV-induced inflammatory factors, thereby alleviating inflammatory responses.

[0009] Seabuckthorn extract is rich in various active ingredients, such as vitamin E and flavonoids. These active ingredients have strong antioxidant properties, effectively scavenging free radicals induced by ultraviolet rays and reducing oxidative damage. They can also absorb ultraviolet rays and protect cell membranes from oxidative damage caused by ultraviolet rays.

[0010] Avocado butter is rich in various active ingredients, such as vitamin E, cinnamate, and unsaturated fatty acids. These active ingredients can prevent allergies caused by light exposure and have a certain repair effect on sunburn and sun spots. They also help maintain the integrity of skin cell membranes and enhance the skin's barrier function.

[0011] Camellia oil is rich in various active ingredients, such as vitamin E, polyphenols, and unsaturated fatty acids. UV radiation can trigger the production of free radicals in the skin, and the active ingredients in camellia oil can scavenge these free radicals, reducing oxidative stress and protecting skin cells. UV radiation can also cause skin inflammation, and the active ingredients in camellia oil can inhibit the production of inflammatory factors and alleviate inflammation.

[0012] Furthermore, the preparation method of the cowhide tea leaf extract comprises the following steps:

[0013] S1, crushing the dried cowhide tea leaves and passing through a 50-80 mesh sieve to obtain cowhide tea leaves powder;

[0014] S2, using 10-15 times the mass of ethanol solution with a concentration of 60-80% v / v to perform ultrasonic-assisted extraction on the cowhide tea powder obtained in S1, filtering to obtain an extract;

[0015] S3, purifying the extract obtained in S2 using a macroporous resin and collecting the eluate;

[0016] S4. Concentrating the eluate obtained in S3 under reduced pressure and spray drying to obtain the cowhide tea leaf extract.

[0017] Furthermore, in step S2, the ultrasonic power is 200-300 W, the ultrasonic frequency is 40-60 kHz, and the extraction time is 1-2 h.

[0018] Furthermore, in step S3, the macroporous resin is one of HPD300, HZ806, and AB-8.

[0019] Furthermore, the step S3 is specifically as follows:

[0020] The pH of the extract is adjusted to 2-2.5 using a hydrochloric acid solution to prepare a loading solution;

[0021] After the sample solution is loaded onto the macroporous resin column, it is eluted with water, 30-40% v / v ethanol solution and 70-80% v / v ethanol solution in sequence at an elution rate of 2-3 BV / h, and the 70-80% v / v ethanol solution eluate is collected.

[0022] Furthermore, the volume ratio of the sample solution to the macroporous resin is 1:2-3; the amount of water is 8-10BV; the amount of 30-40% v / v ethanol solution is 4-6BV; the amount of 70-80% v / v ethanol solution is 2-3BV.

[0023] A sunscreen product contains 1-10 wt% of the above-mentioned anti-ultraviolet damage composition.

[0024] Furthermore, the sunscreen product also includes ethylhexyl triazone, phenylbenzimidazole sulfonic acid and titanium dioxide.

[0025] Compared with the prior art, the present invention has the following beneficial effects:

[0026] The anti-UV damage composition of the present invention includes raw materials such as cowhide tea extract, green algae extract, cornus officinalis extract, sea buckthorn extract, avocado oil, and camellia oil. The composition is rich in multiple active ingredients that work synergistically with each other to neutralize free radicals induced by ultraviolet light, reduce oxidative stress, and protect skin cells from damage. It also absorbs ultraviolet light, reducing its penetration through the skin and thus reducing direct damage to the skin. Furthermore, it regulates tyrosinase activity or blocks the melanin synthesis pathway, reducing melanin accumulation and helping to prevent and improve dark spots and dark spots. DETAILED DESCRIPTION

[0027] In order to better illustrate the purpose, technical solutions and advantages of the present invention, the present invention will be further described below in conjunction with specific embodiments.

[0028] Unless otherwise specified, other materials and reagents used in the examples can be obtained from commercial sources.

[0029] Avocado tree (BUTYROSPERMUM PARKI I) fruit fat: purchased from Guangzhou Rong Daxing Chemical Co., Ltd.

[0030] Camellia oleifera seed oil: purchased from Guangzhou Fuzhilin Biotechnology Co., Ltd.

[0031] Green algae (CHLOROPHYTA SPP.) extract: purchased from Guangzhou Jijia Trading Co., Ltd.;

[0032] Cornus officinalis extract: purchased from Xi'an Zhongkeda Biotechnology Co., Ltd.

[0033] Hippophae rhamnoides extract: purchased from Xi'an Zhongkeda Biotechnology Co., Ltd.

[0034] Example 1 Preparation method of cowhide tea extract

[0035] Experimental Group 1:

[0036] S1, crushing the dried cowhide tea leaves and passing through a 50-mesh sieve to obtain cowhide tea leaves powder;

[0037] S2, using 10 times the mass of 60% v / v ethanol solution to ultrasonically extract the cowhide tea powder obtained in S1, filtering to obtain an extract, wherein the ultrasonic power is 200 W, the ultrasonic frequency is 40 kHz, and the extraction time is 1 hour;

[0038] S3. Adjust the pH of the extract to 2 with hydrochloric acid solution to prepare a loading solution. Load the loading solution onto an AB-8 macroporous resin column and elute with 8 BV of water, 4 BV of 30% v / v ethanol solution, and 2 BV of 70% ethanol solution at a rate of 2 BV / h. Collect the 70% v / v ethanol solution eluate.

[0039] S4. The eluate obtained in S3 is concentrated under reduced pressure at -0.08 MPa and 50° C., and spray-dried at an inlet air temperature of 170° C. and an outlet air temperature of 100° C. to obtain the cowhide tea leaf extract.

[0040] Experimental Group 2:

[0041] S1, crushing the dried cowhide tea leaves and passing through an 80-mesh sieve to obtain cowhide tea leaves powder;

[0042] S2, using 15 times the mass of ethanol solution with a concentration of 80% v / v to perform ultrasonic-assisted extraction on the cowhide tea powder obtained in S1, filtering to obtain an extract, wherein the ultrasonic power is 300 W, the ultrasonic frequency is 60 kHz, and the extraction time is 2 h;

[0043] S3. The pH of the extract was adjusted to 2.5 with hydrochloric acid solution to prepare a loading solution. The loading solution was loaded onto an AB-8 macroporous resin column and eluted with 10 BV of water, 6 BV of 40% v / v ethanol solution, and 3 BV of 80% ethanol solution at a rate of 3 BV / h. The 80% v / v ethanol solution eluate was collected.

[0044] S4. The eluate obtained in S3 is concentrated under reduced pressure at -0.08 MPa and 50° C., and spray-dried at an inlet air temperature of 170° C. and an outlet air temperature of 100° C. to obtain the cowhide tea leaf extract.

[0045] Experimental Group 3:

[0046] S1, crushing the dried cowhide tea leaves and passing through a 60-mesh sieve to obtain cowhide tea leaves powder;

[0047] S2, using 13 times the mass of ethanol solution with a concentration of 70% v / v to perform ultrasonic-assisted extraction on the cowhide tea powder obtained in S1, filtering to obtain an extract, wherein the ultrasonic power is 250 W, the ultrasonic frequency is 50 kHz, and the extraction time is 1.5 h;

[0048] S3. The pH of the extract was adjusted to 2.3 with hydrochloric acid solution to prepare a loading solution. The loading solution was loaded onto an AB-8 macroporous resin column and eluted with 9 BV of water, 5 BV of 35% v / v ethanol solution, and 2 BV of 75% ethanol solution at a rate of 3 BV / h. The 75% v / v ethanol solution eluate was collected.

[0049] S4. The eluate obtained in S3 is concentrated under reduced pressure at -0.08 MPa and 50° C., and spray-dried at an inlet air temperature of 170° C. and an outlet air temperature of 100° C. to obtain the cowhide tea leaf extract.

[0050] Experimental Group 4:

[0051] S1, crushing the dried cowhide tea leaves and passing through a 60-mesh sieve to obtain cowhide tea leaves powder;

[0052] S2, using 13 times the mass and 70% v / v ethanol solution to extract the cowhide tea powder obtained in S1, filtering to obtain an extract, and the extraction time is 5 hours;

[0053] S3. The pH of the extract was adjusted to 2.3 with hydrochloric acid solution to prepare a loading solution. The loading solution was loaded onto an AB-8 macroporous resin column and eluted with 9 BV of water, 5 BV of 35% v / v ethanol solution, and 2 BV of 75% ethanol solution at a rate of 3 BV / h. The 75% v / v ethanol solution eluate was collected.

[0054] S4. The eluate obtained in S3 is concentrated under reduced pressure at -0.08 MPa and 50° C., and spray-dried at an inlet air temperature of 170° C. and an outlet air temperature of 100° C. to obtain the cowhide tea leaf extract.

[0055] Experimental Group 5:

[0056] S1, crushing the dried cowhide tea leaves and passing through a 60-mesh sieve to obtain cowhide tea leaves powder;

[0057] S2, using 13 times the mass of ethanol solution with a concentration of 70% v / v to perform ultrasonic-assisted extraction on the cowhide tea powder obtained in S1, filtering to obtain an extract, wherein the ultrasonic power is 250 W, the ultrasonic frequency is 50 kHz, and the extraction time is 3 h;

[0058] S3. The pH of the extract was adjusted to 2.3 with hydrochloric acid solution to prepare a loading solution. The loading solution was loaded onto an AB-8 macroporous resin column and eluted with 9 BV of water, 5 BV of 35% v / v ethanol solution, and 2 BV of 75% ethanol solution at a rate of 3 BV / h. The 75% v / v ethanol solution eluate was collected.

[0059] S4. The eluate obtained in S3 is concentrated under reduced pressure at -0.08 MPa and 50° C., and spray-dried at an inlet air temperature of 170° C. and an outlet air temperature of 100° C. to obtain the cowhide tea leaf extract.

[0060] Experimental Group 6:

[0061] S1, crushing the dried cowhide tea leaves and passing through a 60-mesh sieve to obtain cowhide tea leaves powder;

[0062] S2, using 13 times the mass of ethanol solution with a concentration of 70% v / v to perform ultrasonic-assisted extraction on the cowhide tea powder obtained in S1, filtering to obtain an extract, wherein the ultrasonic power is 350 W, the ultrasonic frequency is 80 kHz, and the extraction time is 1.5 h;

[0063] S3. The pH of the extract was adjusted to 2.3 with hydrochloric acid solution to prepare a loading solution. The loading solution was loaded onto an AB-8 macroporous resin column and eluted with 9 BV of water, 5 BV of 35% v / v ethanol solution, and 2 BV of 75% ethanol solution at a rate of 3 BV / h. The 75% v / v ethanol solution eluate was collected.

[0064] S4. The eluate obtained in S3 is concentrated under reduced pressure at -0.08 MPa and 50° C., and spray-dried at an inlet air temperature of 170° C. and an outlet air temperature of 100° C. to obtain the cowhide tea leaf extract.

[0065] Experimental Group 7:

[0066] S1, crushing the dried cowhide tea leaves and passing through a 60-mesh sieve to obtain cowhide tea leaves powder;

[0067] S2, using 13 times the mass of ethanol solution with a concentration of 70% v / v to perform ultrasonic-assisted extraction on the cowhide tea powder obtained in S1, filtering to obtain an extract, wherein the ultrasonic power is 250 W, the ultrasonic frequency is 50 kHz, and the extraction time is 1.5 h;

[0068] S3. Adjust the pH of the extract to 2.3 with hydrochloric acid solution to prepare a loading solution. Load the loading solution onto an AB-8 macroporous resin column and elute with 9 BV of 20% v / v ethanol solution, 5 BV of 35% v / v ethanol solution, and 2 BV of 75% ethanol solution, respectively, at an elution rate of 3 BV / h. Collect the 75% v / v ethanol solution eluate.

[0069] S4. The eluate obtained in S3 is concentrated under reduced pressure at -0.08 MPa and 50° C., and spray-dried at an inlet air temperature of 170° C. and an outlet air temperature of 100° C. to obtain the cowhide tea leaf extract.

[0070] Experimental Group 8:

[0071] S1, crushing the dried cowhide tea leaves and passing through a 60-mesh sieve to obtain cowhide tea leaves powder;

[0072] S2, using 13 times the mass of ethanol solution with a concentration of 70% v / v to perform ultrasonic-assisted extraction on the cowhide tea powder obtained in S1, filtering to obtain an extract, wherein the ultrasonic power is 250 W, the ultrasonic frequency is 50 kHz, and the extraction time is 1.5 h;

[0073] S3. Adjust the pH of the extract to 4 with hydrochloric acid solution to prepare a loading solution. Load the loading solution onto an AB-8 macroporous resin column and elute with 9 BV of water, 5 BV of 35% v / v ethanol solution, and 2 BV of 75% ethanol solution, respectively, at a rate of 3 BV / h. Collect the 75% v / v ethanol solution eluate.

[0074] S4. The eluate obtained in S3 is concentrated under reduced pressure at -0.08 MPa and 50° C., and spray-dried at an inlet air temperature of 170° C. and an outlet air temperature of 100° C. to obtain the cowhide tea leaf extract.

[0075] Test Example 1

[0076] The cowhide tea extracts obtained from experimental groups 1-8 were placed at a high temperature of 45°C for 72 hours. The cowhide tea extracts at 0 days and 72 hours were respectively used for ABTS free radical scavenging test and UV radiation resistance test.

[0077] 1. ABTS free radical scavenging test method:

[0078] (1) Add ABTS to deionized water to prepare a 7 mmol / L ABTS stock solution.

[0079] (2) Potassium persulfate was added to deionized water to prepare a 140 mmol / L potassium persulfate stock solution.

[0080] (3) ABTS stock solution and potassium persulfate stock solution were mixed in a 1:1 ratio. After reacting in the dark for 12 hours, the working solution was diluted with anhydrous ethanol until the absorbance of the solution was 0.7 ± 0.1.

[0081] (4) The cowhide tea leaf extract was diluted 10 times with 95% v / v ethanol to prepare a sample solution.

[0082] (5) Prepare a test sample by mixing the sample solution and the working solution in a mass ratio of 1:4. Let the sample stand for 6 minutes and measure the absorbance at 734 nm. Perform three parallel tests on the sample.

[0083] ABTS free radical scavenging rate = [A-(BC)] / A × 100%

[0084] Wherein, A is the blank control (using deionized water instead of the sample solution); B is the test sample; and C is the sample solution.

[0085] 2. Determination of UV radiation resistance:

[0086] (1) Take the cowhide tea extracts prepared in experimental groups 1-8, and dilute the cowhide tea extracts of each group 50 times with 50% ethanol solution to prepare sample solutions.

[0087] (2) An ethanol solution with a volume concentration of 50% was used as the control solution.

[0088] (3) The sample solution and the control solution were scanned in the wavelength range of 280-400 nm using a UV-visible spectrophotometer. Three parallel tests were performed for each experimental group, and the average absorbance values at UVB (280-320 nm) and UVA (320-400 nm) were calculated.

[0089]

[0090] The experimental results of ABTS free radical scavenging test show that the cowhide tea extract obtained by the preparation method of the present invention has a good ABTS free radical scavenging effect. Combined with the anti-ultraviolet radiation ability determination and analysis, the cowhide tea extract has a good absorption effect on ultraviolet rays in the 280-400nm band, indicating that the cowhide tea extract has a strong antioxidant capacity and can reduce the damage to the skin caused by ultraviolet radiation. After high temperature testing at 45°C, the antioxidant capacity of the cowhide tea extracts obtained in experimental groups 1-3 changed little. The ultrasonic parameters and resin purification extraction process were adjusted in experimental groups 4-8, resulting in a decrease in the ABTS free radical scavenging effect. Among them, ultrasonic treatment was not used in experimental group 4, resulting in a decrease in the ABTS free radical scavenging effect and anti-ultraviolet radiation ability of the cowhide tea extract. The use of reasonable ultrasonic parameters to treat the cowhide tea in the present invention can promote the release and dissolution of active ingredients in the cowhide tea; the cowhide tea extract obtained by extending the extraction time in experimental group 5 and increasing the ultrasonic frequency in experimental group 6 The extracts showed comparable ABTS free radical scavenging effects and UV radiation resistance to those of experimental groups 1-3. However, the ultrasonic parameters used in experimental groups 5-6 resulted in increased energy consumption compared to those of experimental groups 1-3. Changing the type of eluent in experimental group 7 not only reduced the ABTS free radical scavenging effect of the cowhide tea extract, but also significantly decreased after being placed at a high temperature of 45°C for 72 hours. Changing the pH value of the sample solution in experimental group 8 resulted in a decrease in the purification effect. However, adjusting the pH value of the sample solution to 2-2.5 in the present invention improved the adsorption of the active ingredient by the resin. Therefore, the cowhide tea extract obtained in experimental group 3 was selected as the raw material for the composition.

[0091] Example 2 Composition for resisting ultraviolet damage

[0092] Treatment Group 1:

[0093] The anti-ultraviolet damage composition includes the following raw materials by weight: 5 parts of cowhide tea leaf extract (experimental group 3), 3 parts of green algae extract, 1 part of cornus officinalis extract, 8 parts of sea buckthorn extract, 2.5 parts of avocado tree fat and 5 parts of camellia oil.

[0094] Treatment Group 2:

[0095] The anti-ultraviolet damage composition includes the following raw materials by weight: 8 parts of cowhide tea leaf extract (experimental group 3), 7 parts of green algae extract, 4 parts of cornus officinalis extract, 10 parts of sea buckthorn extract, 6 parts of avocado tree fat and 8 parts of camellia oil.

[0096] Treatment Group 3:

[0097] The anti-ultraviolet damage composition includes the following raw materials by weight: 6.5 parts of cowhide tea leaf extract (experimental group 3), 5 parts of green algae extract, 2.5 parts of cornus officinalis extract, 9 parts of sea buckthorn extract, 4 parts of avocado tree fat and 7 parts of camellia oil.

[0098] Treatment Group 4:

[0099] The only difference from treatment group 3 is that it does not contain cowhide tea leaf extract, and the missing amount is made up by green algae extract, cornus officinalis extract, and sea buckthorn extract in a mass ratio of 5:2.5:9.

[0100] Treatment Group 5:

[0101] The only difference from treatment group 3 is that the green algae extract is not included, and the missing amount is made up by cowhide tea extract, cornus officinalis extract, and sea buckthorn extract in a mass ratio of 6.5:2.5:9.

[0102] Treatment Group 6:

[0103] The only difference from treatment group 3 is that: Cornus officinalis extract is not included, and the missing amount is made up by cowhide tea extract, green algae extract, and sea buckthorn extract in a mass ratio of 6.5:5:9.

[0104] Treatment Group 7:

[0105] The only difference from treatment group 3 is that sea buckthorn extract is not included, and the missing amount is made up by cowhide tea leaf extract, green algae extract, and cornus officinalis extract in a mass ratio of 6.5:5:2.5.

[0106] Test Example 2

[0107] Tyrosinase inhibition test process:

[0108] (1) Tyrosinase and L-tyrosine solutions were prepared using PBS buffer to prepare 500 U / mL tyrosinase solution and 0.5 g / L L-tyrosine solution, respectively.

[0109] (2) The compositions of treatment groups 1-7 were prepared into 1 wt% test samples using PBS buffer.

[0110] (3) Each treatment group was tested using 12 wells. 50 μL of tyrosine solution was added to each well, and the cells were then divided equally into groups A, B, C, and D, with 3 wells in each group. Group A added 100 μL of deionized water and 20 μL of L-tyrosine solution; Group B added 120 μL of deionized water; Group C added 100 μL of the test sample and 20 μL of L-tyrosine solution; and Group D added 120 μL of the test sample. The cells were reacted at 25°C for 10 min, and the absorbance was measured at 475 nm to calculate the tyrosinase inhibition rate.

[0111] Tyrosinase inhibition rate = (AB) - (CD) / AB × 100%

[0112] Melanin inhibition test:

[0113] Tested in accordance with T / SHRH 036-2021 "Cosmetic Melanin Inhibition Test - Zebrafish Embryo Test Method".

[0114] The embryo culture medium was prepared by weighing 17.5 g of NaCl, 0.76 g of KCl, 2.91 g of CaCl2, and 2.38 g of MgSO4, adding 300 mL of deionized water and mixing, then adding 0.714 g of HEPES, and adjusting the pH to 7.2 with 1 mol / L NaOH to prepare the embryo culture medium.

[0115] The compositions of treatment groups 1-7 were each prepared with water to a 1 wt% concentration of the test sample. Each group consisted of 30 zebrafish embryos, with three replicates performed. The experimental group consisted of zebrafish embryos placed in the test sample, the positive control group consisted of zebrafish embryos placed in a 0.03 mg / mL phenylthiourea solution, and the blank group consisted of zebrafish embryos placed in embryo culture medium. After 48 hours of exposure in a 28°C incubator, the melanin signal intensity of the fish embryos was measured and statistically analyzed.

[0116] Melanin inhibition rate (%) = (average value of “average signal intensity” of fish embryos in the blank group - average value of “average signal intensity” of fish embryos in the experimental group) / (average value of “average signal intensity” of fish embryos in the blank group - average value of “average signal intensity” of fish embryos in the positive control group) × 100%

[0117] name Tyrosinase inhibition rate (%) Melanin inhibition rate (%) Treatment group 1 83.4 26.2 Treatment group 2 84.1 25.7 Treatment group 3 84.5 27.1 Treatment group 4 73.4 22.4 Treatment group 5 76.8 21.9 Treatment group 6 74.6 23.8 Treatment group 7 78.2 20.5

[0118] The test results show that the composition of the present invention can effectively inhibit the activity of tyrosinase, inhibit the production of melanin, and prevent the skin from undergoing a melanization reaction after being irradiated by ultraviolet rays.

[0119] Example 3 Sunscreen product

[0120] The composition of treatment group 3 was used to prepare a sunscreen product according to the raw material list below:

[0121]

[0122] Comparative Example 1

[0123] On the basis of Example 3, the composition of Treatment Group 3 was not added, and the missing amount was made up by an equal amount of deionized water. The rest was the same as Example 3.

[0124] Preparation process of sunscreen products of Example 3 and Comparative Example 1:

[0125] Preparation of oil phase: Add the oil phase components into the oil phase pot, heat to 70°C, and stir evenly to fully dissolve and disperse the components to form a uniform oil phase.

[0126] Preparation of aqueous solution phase: Add the aqueous phase components, the composition of treatment group 3 and deionized water into the aqueous phase pot, heat to 75-80°C, stir evenly to fully dissolve the components and form a uniform aqueous solution phase.

[0127] Emulsification: Slowly add the aqueous phase to the oil phase, stirring while adding. Control the stirring speed to ensure that the oil and water phases are fully mixed to form colostrum. Homogenize at 4000 rpm for 4 minutes to prepare the sunscreen.

[0128] The finished product shall be tested for SPF value and PFA value in accordance with the test method for sunscreen cosmetics sun protection index (SPF value) and the test method for long-wave ultraviolet protection index (PFA value) of sunscreen cosmetics in the "Technical Specifications for Safety of Cosmetics".

[0129] name PFA SPF Example 3 11 58.9 Comparative Example 1 7 42.7

[0130] Experimental results show that the sunscreen prepared from the composition of the present invention has a good protective effect.

[0131] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and are not intended to limit the scope of protection of the present invention. Although the present invention has been described in detail with reference to preferred embodiments, those skilled in the art should understand that the technical solutions of the present invention may be modified or replaced by equivalents without departing from the essence and scope of the technical solutions of the present invention.

Claims

1. A composition for resisting ultraviolet damage, characterized in that, The composition comprises the following raw materials in parts by weight: 5-8 parts of cowhide tea leaf extract, 3-7 parts of green algae extract, 1-4 parts of cornus officinalis extract, 8-10 parts of sea buckthorn extract, 2.5-4 parts of avocado tree butter and 5-8 parts of camellia oil.

2. The composition for resisting ultraviolet damage according to claim 1, wherein The preparation method of the cowhide tea leaf extract comprises the following steps: S1, crushing the dried cowhide tea leaves and passing through a 50-80 mesh sieve to obtain cowhide tea leaves powder; S2, using 10-15 times the mass of ethanol solution with a concentration of 60-80% v / v to perform ultrasonic-assisted extraction on the cowhide tea powder obtained in S1, filtering to obtain an extract; S3, purifying the extract obtained in S2 using a macroporous resin and collecting the eluate; S4. Concentrating the eluate obtained in S3 under reduced pressure and spray drying to obtain the cowhide tea leaf extract.

3. The composition for resisting ultraviolet damage according to claim 2, wherein In step S2, the ultrasonic power is 200-300 W, the ultrasonic frequency is 40-60 kHz, and the extraction time is 1-2 h.

4. The composition for resisting ultraviolet damage according to claim 2, characterized in that The macroporous resin in step S3 is one of HPD300, HZ806, and AB-8.

5. The composition for resisting ultraviolet damage according to claim 2, characterized in that The step S3 is specifically as follows: The pH of the extract is adjusted to 2-2.5 using a hydrochloric acid solution to prepare a loading solution; After the sample solution is loaded onto the macroporous resin column, it is eluted with water, 30-40% v / v ethanol solution and 70-80% v / v ethanol solution in sequence at an elution rate of 2-3 BV / h, and the 70-80% v / v ethanol solution eluate is collected.

6. The composition for resisting ultraviolet damage according to claim 5, characterized in that The volume ratio of the sample solution to the macroporous resin is 1:2-3; the amount of water is 8-10BV; the amount of 30-40% v / v ethanol solution is 4-6BV; the amount of 70-80% v / v ethanol solution is 2-3BV.

7. Use of the anti-ultraviolet damage composition according to any one of claims 1 to 6 in sunscreen products.

8. A sunscreen product, characterized in that: A composition for resisting ultraviolet damage comprising 1-10 wt % of any one of claims 1-6.

9. The sunscreen product according to claim 8, characterized in that Also included are ethylhexyl triazone, phenylbenzimidazole sulfonic acid, and titanium dioxide.

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