A moisturizing antioxidant composition containing a guettarda speciosa extract and pdrn

Abstract

The application discloses a moisturizing and antioxidant composition containing a malpighia emarginata tree extract and PDNR and belongs to the technical field of cosmetics. The composition comprises the following components: a malpighia emarginata tree bark extract, a chrysophyllum coccineum fruit extract, a adenium obesum leaf cell extract, a cornus officinalis fruit extract and polydeoxyribonucleotide (PDRN), and the composition has good moisturizing and antioxidant effects through the synergistic effect among the five components.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of cosmetics, in particular to a moisturizing and antioxidant composition containing Euphorbia neriifolia extract and PDRN. BACKGROUND

[0002] Skin oxidative stress is a core pathological factor that accelerates aging, which leads to collagen degradation and skin barrier function decline through active oxygen (ROS)-mediated cell damage pathways. Currently, natural plant extracts (such as polyphenols and terpenoids) are widely used in anti-aging cosmetics due to their antioxidant activity. However, single plant active ingredients have the following significant limitations in application:

[0003] Limited target: single component cannot comprehensively cover the multi-pathway damage mechanism of oxidative stress, resulting in insufficient antioxidant efficacy;

[0004] High effective dose: high concentration is often required to achieve the desired effect, which may cause skin irritation or formulation stability problems;

[0005] Lack of synergistic mechanism: conventional compounding schemes rely on simple combination, and there is a lack of synergistic effect between components, making it difficult to break through the efficacy bottleneck.

[0006] In addition, the synergistic application of plant extracts and bioactive ingredients (such as nucleic acid substances) in the prior art is not sufficient, especially lacking a solution that can simultaneously optimize antioxidant and moisturizing efficacy and is suitable for sensitive skin. Therefore, it is urgent to develop a compounding system that achieves multi-component synergistic effect through scientific proportioning, to break through the limitations of existing technology with low dose and multi-target strategy. SUMMARY

[0007] The purpose of the present application is to provide a moisturizing and antioxidant composition containing Euphorbia neriifolia extract and PDRN, which uses natural plant extracts and PDRN for compounding, has high safety, and has significant moisturizing and antioxidant efficacy.

[0008] In order to achieve the above purpose, the present application adopts the following technical solutions:

[0009] In the first aspect, the present application provides a moisturizing and antioxidant composition containing Euphorbia neriifolia extract, which comprises Euphorbia neriifolia bark extract, Syagrus romanzoffiana fruit extract, Ochrocarpus desertus leaf cell extract, and PDRN in a mass ratio of 1:0.8-1:2-3:1-2:1-1.5.

[0010] In the second aspect, the present application provides the use of the moisturizing and antioxidant composition containing Euphorbia neriifolia extract in the first aspect in the preparation of cosmetics with moisturizing and antioxidant efficacy.

[0011] Preferably, the dosage form of the cosmetic is any one of a cream, an essence.

[0012] In a third aspect, the present application provides an essence with moisturizing and antioxidant effects, the essence with moisturizing and antioxidant effects is composed of the following components in mass percentage:

[0013] The moisturizing and antioxidant composition containing the Euphorbia maculata extract in the first aspect: 4-6wt%;

[0014] Dipotassium glycyrrhizinate: 0.1-0.3wt%;

[0015] Hydroxypropyl methyl cellulose: 0.5-1.5wt%;

[0016] Deionized water is supplemented to 100wt%.

[0017] In a fourth aspect, the present application provides a preparation method of the essence in the third aspect, comprising the following steps:

[0018] A1: the Euphorbia maculata bark extract, the annona glabra fruit extract, the adenium obesum leaf cell extract, the cornus officinalis fruit extract, the dipotassium glycyrrhizinate and the hydroxypropyl methyl cellulose are mixed uniformly using 2 / 3 of deionized water at 50-60℃, and then cooled to 2-8℃ to obtain a mixed solution A;

[0019] A2: the PDRN is mixed using the remaining deionized water at 2-8℃ to obtain a mixed solution B;

[0020] A3: the mixed solution A and the mixed solution B are mixed uniformly at 2-8℃ to obtain the essence, and the essence is sealed using an ampoule bottle and stored at 2-8℃ in the dark.

[0021] Compared with the prior art, the present application has the following beneficial effects:

[0022] The present application synchronously breaks through the antioxidant and moisturizing effects through the synergistic matching of active ingredients and low-temperature process innovation, and takes into account the safety and universality, and provides an efficient solution for sensitive skin care, as follows:

[0023] 1. Synergistic antioxidant effect

[0024] The Euphorbia maculata bark extract, the annona glabra fruit extract, the adenium obesum leaf cell extract, the cornus officinalis fruit extract and the PDRN are compounded according to a specific mass ratio in the present application, a synergistic mechanism is formed, the antioxidant efficiency is significantly improved, and there is no negative effect on skin cell activity.

[0025] 2. Long-acting moisturizing optimization

[0026] The essence liquid of the application is prepared by low-temperature step-by-step premixing and then mixing, so that the prepared essence liquid has significantly enhanced skin absorption efficiency and achieves long-lasting hydration and moisturizing effect.

[0027] 3. Security universality

[0028] The essence liquid of the application is mild and non-irritating, suitable for sensitive skin people, and has high biological safety and stability. DETAILED DESCRIPTION

[0029] In order to better understand the present application, the present application will be further described below in combination with specific examples, wherein the terms used in the examples are used for describing specific specific embodiments, and do not constitute a limitation on the protection scope of the present application.

[0030] The experimental methods in the following tests without specific conditions are usually according to the conventional conditions, or according to the conditions suggested by the manufacturers. Unless otherwise specified, the percentages and parts are calculated by weight.

[0031] Part of the raw materials and sources are as follows:

[0032] PDRN: purchased from Regenesis (Shandong) Biotechnology Co., Ltd., model: RJMPDRN-850K.

[0033] Pachysandra terminalis bark: purchased from Shanghai Qianfei Chemical Co., Ltd.

[0034] Annaspiro extract: purchased from Shanghai Yanganfu Industry Co., Ltd.

[0035] Desert rose leaf cell extract: purchased from Shanghai Xinsengmeike Biological Co., Ltd.

[0036] Fructus corni: purchased from Guangzhou Nuoyuan Biological Technology Co., Ltd.

[0037] Other raw materials or reagents are all conventional market sales.

[0038] The preparation method of the Pachysandra terminalis bark extract disclosed in the application refers to the preparation method of Pachysandra terminalis bark extract 1 disclosed in the patent "Antioxidant composition containing Pachysandra terminalis bark extract and application thereof" (application number: CN202510334360.3), and the specific steps are as follows:

[0039] S1. Dry the Pachysandra terminalis bark to a water content of ≤5%, and grind it into coarse powder;

[0040] S2. Mix the Pachysandra terminalis bark coarse powder with rapeseed oil and polyglyceryl-3 diisostearate, heat to 55℃, and stir at 300rpm for 20min to obtain a mixed liquid;

[0041] S3. Under the atmosphere of N2, the mixture in S2 is subjected to ultrasonic-assisted extraction to obtain an extract, the extraction temperature is 55 DEG C, the ultrasonic frequency is 30 kHz, and the extraction time is 45 min;

[0042] S4. The extract is filtered to remove insoluble substances, centrifuged, and the supernatant is taken to obtain the E. punctata bark extract.

[0043] In step S2, the mass ratio of the E. punctata bark coarse powder, rapeseed oil and polyglycerol-3 diisostearate is 1:3:0.2.

[0044] The preparation method of the Cornus officinalis fruit extract is based on the preparation method of the Morroniside-rich Cornus officinalis fruit extract disclosed in the embodiments of the patent “Morroniside-rich Cornus officinalis fruit extract, preparation method thereof and application” (application number: CN202510000746.0), and the specific steps are as follows:

[0045] (1) A certain amount of Cornus officinalis fruit medicinal material (produced in Qinling, Shaanxi) is dried and crushed into powder for use. 1 kg of 60% ethanol is weighed according to a solid-liquid ratio of 1:10, and then added into an extraction tank after adjusting the pH to 5. The Cornus officinalis fruit powder is then added and stirred to obtain a mixture to be extracted.

[0046] (2) 0.5% of pectinase and cellulase based on the dry weight of the Cornus officinalis fruit are added to the mixture to be extracted, and the mass ratio of the pectinase to the cellulase is 1:1. First, the mixture is extracted at a constant temperature of 50 DEG C for 1 h, and then the temperature is increased to 70 DEG C for 1 h. Then, the temperature is increased to 90 DEG C for 30 min to inactivate the enzymes to obtain a crude extract. Then, the temperature is decreased to 50 DEG C, and the crude extract is discharged into a storage tank. The crude extract is centrifuged, and the filtrate is collected and the filter residue is discarded. The filtrate is added into a concentrator in three batches for concentration, and then centrifuged to obtain a crude extract. It should be noted that the cyclic extraction refers to an operation of promoting the contact between the material and the solvent through stirring, shaking and the like.

[0047] (3) The crude extract is diluted with 10 times pure water, and then added into a chromatographic column filled with a polyamide filler layer for chromatography. Then, the column is eluted with pure water, wherein the flow rate of the sample loading is controlled at 2 times the column volume / h, and 6 times the column volume of pure water is used for elution at a flow rate of 2 times the column volume / h, and the eluate is collected from the start of the sample loading. The collected eluate is added into a concentrator for concentration for 3 times, and then centrifuged to obtain the Cornus officinalis fruit extract. The pH value of the Cornus officinalis fruit extract is 5.6, and the relative density is 1.084.

[0048] The mass ratio of each component in the composition with moisturizing and antioxidant effects is as follows:

[0049] Composition 1:

[0050] The extract of the bark of Pachysandra terminalis, the extract of the fruit of Pseudowintera colorata, the extract of the leaf cell of Adenium obesum, the extract of the fruit of Cornus officinalis, and PDRN are in a mass ratio of 1:0.9:2.5:1.5:1.3.

[0051] Composition 2:

[0052] The extract of the bark of Pachysandra terminalis, the extract of the fruit of Pseudowintera colorata, the extract of the leaf cell of Adenium obesum, the extract of the fruit of Cornus officinalis, and PDRN are in a mass ratio of 1:1:3:2:1.

[0053] Composition 3:

[0054] The extract of the bark of Pachysandra terminalis, the extract of the fruit of Pseudowintera colorata, the extract of the leaf cell of Adenium obesum, the extract of the fruit of Cornus officinalis, and PDRN are in a mass ratio of 1:0.8:2:1:1.5.

[0055] Composition ①:

[0056] Different from Composition 1, Composition ① does not contain the extract of the bark of Pachysandra terminalis, and is composed of the extract of the fruit of Pseudowintera colorata, the extract of the leaf cell of Adenium obesum, the extract of the fruit of Cornus officinalis, and PDRN in a mass ratio of 0.9:2.5:1.5:1.3; the rest of the raw materials are the same as those in Composition 1.

[0057] Composition ②:

[0058] Different from Composition 1, Composition ② does not contain the extract of the fruit of Pseudowintera colorata, and is composed of the extract of the bark of Pachysandra terminalis, the extract of the leaf cell of Adenium obesum, the extract of the fruit of Cornus officinalis, and PDRN in a mass ratio of 1:2.5:1.5:1.3; the rest of the raw materials are the same as those in Composition 1.

[0059] Composition ③:

[0060] Different from Composition 1, Composition ③ does not contain the extract of the leaf cell of Adenium obesum, and is composed of the extract of the bark of Pachysandra terminalis, the extract of the fruit of Pseudowintera colorata, the extract of the fruit of Cornus officinalis, and PDRN in a mass ratio of 1:0.9:1.5:1.3; the rest of the raw materials are the same as those in Composition 1.

[0061] Composition ④:

[0062] Different from Composition 1, Composition ④ does not contain the extract of the fruit of Cornus officinalis, and is composed of the extract of the bark of Pachysandra terminalis, the extract of the fruit of Pseudowintera colorata, the extract of the leaf cell of Adenium obesum, and PDRN in a mass ratio of 1:0.9:2.5:1.3; the rest of the raw materials are the same as those in Composition 1.

[0063] Composition ⑤:

[0064] Different from composition 1, composition 5 does not contain PDRN, which is composed of Euphorbia tirucalli bark extract, annona glabra fruit extract, adenium obesum leaf cell extract and cornus officinalis fruit extract with a mass ratio of 1:0.9:2.5:1.5, and the rest of the raw materials are the same as composition 1.

[0065] Composition 6:

[0066] Different from composition 1, composition 6 is composed of Euphorbia tirucalli bark extract, annona glabra fruit extract, adenium obesum leaf cell extract, cornus officinalis fruit extract and PDRN with a mass ratio of 1:0.5:1:3:2.

[0067] The mass percentage and preparation steps of each component in the essence with moisturizing and antioxidant effects according to the present application are as follows:

[0068] Essence 1: composition 1: 6wt%, dipotassium glycyrrhizinate: 0.3wt%, hydroxypropyl methyl cellulose: 1.5wt%, and deionized water is supplemented to 100wt%;

[0069] Preparation method:

[0070] A1: Euphorbia tirucalli bark extract, annona glabra fruit extract, adenium obesum leaf cell extract, cornus officinalis fruit extract, dipotassium glycyrrhizinate and hydroxypropyl methyl cellulose are mixed uniformly with 2 / 3 of deionized water at 55℃, and then cooled to 8℃ to obtain a mixed solution A;

[0071] A2: PDRN is mixed with the remaining deionized water at 8℃ to obtain a mixed solution B;

[0072] A3: The mixed solution A and the mixed solution B are mixed uniformly at 8℃ to obtain an essence, which is sealed and stored at 8℃ in the dark.

[0073] Essence 2: composition 2: 5wt%, dipotassium glycyrrhizinate: 0.2wt%, hydroxypropyl methyl cellulose: 1wt%, and deionized water is supplemented to 100wt%;

[0074] The preparation steps are the same as those of essence 1.

[0075] Essence 3: composition 3: 4wt%, dipotassium glycyrrhizinate: 0.1wt%, hydroxypropyl methyl cellulose: 0.5wt%, and deionized water is supplemented to 100wt%;

[0076] The preparation steps are the same as those of essence 1.

[0077] Essence 1: composition 1: 6wt%, dipotassium glycyrrhizinate: 0.3wt%, hydroxypropyl methyl cellulose: 1.5wt%, and deionized water is supplemented to 100wt%;

[0078] Preparation method:

[0079] Deionized water was mixed with each component in Composition 1, dipotassium glycyrrhizinate, and hydroxypropyl methylcellulose at 55°C, and cooled to 8°C to obtain an essence, which was sealed and stored at 8°C in the dark.

[0080] Test 1: Antioxidant test determination

[0081] 1-1: ABTS inhibition rate determination

[0082] ABTS reacts with potassium persulfate to generate green ABTS·. + free radicals. This radical has a maximum absorption at 752 nm. Therefore, by detecting the absorbance at 752 nm, the concentration can be determined. If a substance is added to the ABTS· + free radical working solution, and the absorbance at 752 nm decreases, it indicates that the substance has free radical scavenging activity and is an antioxidant.

[0083] Test samples: Composition 1-3, Composition ①-⑥.

[0084] Test sample preparation: The test sample was prepared into a mixture of 0.1 mg / mL using deionized water.

[0085] The sample solution was detected using a total antioxidant capacity (ABTS method) test kit (purchased from Shanghai Zhiyan, model ml092653), and the detection method strictly followed the kit instructions.

[0086] The experimental results are shown in Table 1.

[0087] Table 1: Effect of composition on ABST clearance rate

[0088] Sample name Radical ABST clearance rate (%) Composition 1 48.33 Composition 2 46.93 Composition 3 46.81 Composition 1 36.57 Composition 2 33.76 Composition 3 27.42 Composition 4 36.81 Composition 5 36.59 Composition 6 41.31

[0089] As can be seen from the results of Composition 1-3, the antioxidant effect of Composition 1 is the best, so Composition 1 is selected as the best embodiment.

[0090] From the results of comparing composition 1 with compositions ①-⑥, it can be seen that in the composition provided by the present application, the four components of Euclea natalensis bark extract, Annona glabra fruit extract, Adenium obesum leaf cell extract and Cornus officinalis fruit extract have a significant synergistic effect with PDRN. Specifically, from the results of comparing composition 1 with compositions ①-⑤, it can be seen that there is a certain synergistic effect between the Euclea natalensis bark extract, Annona glabra fruit extract, Adenium obesum leaf cell extract and Cornus officinalis fruit extract in the present application; from the results of comparing composition 1 with composition ⑥, it can be seen that the composition obtained by compounding the Euclea natalensis bark extract, Annona glabra fruit extract, Adenium obesum leaf cell extract and Cornus officinalis fruit extract with PDRN in the mass ratio defined in the present application has a better ABST free radical scavenging rate.

[0091] 1-2: Composition ROS scavenging of human keratinocytes

[0092] Sample solution: The sample solution is a mixture prepared by using Promocell special complete culture medium to prepare compositions 1-3 and compositions ①-⑥ into a mixture with a concentration of 5 mg / mL.

[0093] Cell culture: Human primary skin keratinocytes (NEKs) are isolated from normal skin tissue and cultured in a Promocell special complete culture medium (Keratinocyte Growth Medium 2, C-20011) at 37°C, 5% CO2, and subcultured every 5 days when the cells grow to near confluence.

[0094] Cell viability detection: Take NEKs cells in the best growth state, and perform routine treatment. The cell suspension density is adjusted to 8×10 4 -1×10 5 μL of the cell suspension per well in a 96-well plate, and placed in a 37°C, 5% CO2 incubator. Add 50 μL of the sample solution and incubate for 24 h. Set up a negative control group (equivalent amount of cell culture medium instead of sample solution) and a blank control group (no cell suspension inoculation, only add cell culture medium). Each experimental group has 3 parallel holes. After 24 h of culture, add 10 μL of CCK-8 reagent to each well, and incubate for 2 h. Use a microplate reader to measure the absorbance at 450 nm, and the reference wavelength is 600 nm or above.

[0095] ROS detection: Take NEKs cells in the best growth state, and use Promocell special culture medium for culture. The cell suspension density is adjusted to 8×10 4 -1×10 5The cells were inoculated in 96-well plates at 100 μL per well with a cell suspension, and were placed in a 37°C, 5% CO2 incubator. A final concentration of 10 μmol / L vitamin K3 was added to each reaction well, and 50 μL of sample solution was added to the experimental group, 50 μL of vitamin E solution (solvent: cell culture medium, final concentration of vitamin E in the reaction well: 200 μmol / L) was added to the positive group, and 50 μL of cell culture medium was added to the blank group. After 24 h of culture, 1 μL of ROS fluorescent coloring reagent (CellRox, Thermo) was added to each well, and conventional incubation was performed for 4 h. The fluorescence value was measured using an enzyme-labeled instrument, the excitation wavelength was 485 nm, and the emission wavelength was 520 nm.

[0096] Relative cell viability / % = (test group detection value - blank group detection value) / (negative group detection value - blank group detection value) x 100%

[0097] The test results are expressed as mean values, as shown in Table 2.

[0098] ROS inhibition rate / % = (blank group fluorescence intensity - experimental group or positive group fluorescence intensity) / blank group fluorescence intensity x 100%

[0099] The test results are expressed as mean values, as shown in Table 3.

[0100] Table 2 Effect of the composition on NEKs activity

[0101] Group Cell viability / % Negative group 100 Composition 1 98.42 Composition 2 99.73 Composition 3 99.64 Composition 1 98.19 Composition 2 98.76 Composition 3 97.42 Composition 4 97.98 Composition 5 98.51 Composition 6 99.36

[0102] According to the results in Table 2, the composition has little effect on the cell viability of NEKs, and therefore, the next ROS detection test is performed.

[0103] Table 3 Effect of the composition on ROS inhibition rate

[0104] Group ROS inhibition rate / % Composition 1 34.57 Composition 2 33.59 Composition 3 32.41 Composition 1 20.16 Composition 2 22.31 Composition 3 21.52 Composition 4 20.73 Composition 5 22.48 Composition 6 28.19 Vitamin E 45.73

[0105] According to Table 3, the results of compositions 1-3 show that the ROS inhibition rate of composition 1 is the best, and therefore, composition 1 is selected as the best embodiment.

[0106] From the ROS inhibition rate results of composition 1 and compositions ①-⑥, it can be seen that in the composition provided by the application, there is a significant synergistic effect between the bark extract of Eucryphia lucida, the fruit extract of Annona glabra, the leaf cell extract of Adenium obesum, the fruit extract of Cornus officinalis and PDRN. Specifically, from the results of composition 1 and compositions ①-⑤, it can be seen that there is a certain synergistic effect between the bark extract of Eucryphia lucida, the fruit extract of Annona glabra, the leaf cell extract of Adenium obesum, the fruit extract of Cornus officinalis and PDRN in the application; from the results of composition 1 and composition ⑥, it can be seen that the composition obtained by compounding the bark extract of Eucryphia lucida, the fruit extract of Annona glabra, the leaf cell extract of Adenium obesum, the fruit extract of Cornus officinalis and PDRN in the quality ratio defined in the application has better ROS inhibition rate effect.

[0107] Test 2: Human safety test

[0108] 2-1. Human skin patch test

[0109] Test substances: serum 1-3, serum ①.

[0110] Selection of subjects: healthy volunteers aged 21-57 years old were selected, who voluntarily participated and signed the informed consent, could complete the test during the test period according to the requirements, and were randomly divided into groups, with 30 subjects in each group.

[0111] Experimental method: qualified patch equipment was selected, and the closed patch test method was used. 1g of the test substance was placed in the patch equipment and evenly coated, and then the low-sensitization adhesive tape was applied to the flexor of the forearm of the subject. After 24 hours, the test substance was removed, and the skin reaction was observed and recorded at 0.5, 24 and 48 hours after removal, respectively.

[0112] Result evaluation: the skin adverse reaction grading was determined according to the skin adverse reaction grading of human trial test in the “Cosmetic Safety Technology Specification” (2015 edition), and the grading standard is shown in Table 4, and the results are shown in Table 5.

[0113] Table 4 Skin adverse reaction grading standard

[0114] Grade classification Phenomenon 0 level Skin has no reaction 1 level Skin appears pinkish red spots 2 level Skin appears red spots, infiltration and papules 3 level Skin appears red spots, edema, papules and blisters 4 level Skin appears red spots, edema and bullae

[0115] Table 5 Results of human skin patch test

[0116]

[0117] 2-2. Human application test for sensitive skin

[0118] Test substances: serum 1-3, serum ①.

[0119] Subject selection: Healthy volunteers aged 21-44 years old, positive in lactic acid stinging test, voluntarily participate and sign the informed consent, can complete the test as required during the test period, randomly grouped, 30 subjects in each group.

[0120] Experimental method: Volunteers randomly divide the bilateral face into test side and control side, after cleansing, evenly apply the product to the facial skin of the test side according to the product usage method, and the control side does not use any product. After 28 days of trial, the volunteers feedback the local skin reaction, and if there is an adverse reaction, the dermatologist evaluates the adverse reaction.

[0121] Result evaluation: The skin adverse reaction grading is determined according to the skin adverse reaction grading of human trial test in the “Cosmetic Safety Technology Specification” (2015 edition), and the grading standard is shown in Table 4, and the results are shown in Table 6.

[0122] Table 6 Results of human trial test

[0123]

[0124] From the results of human skin patch test, during the test period, essence 1-3 and essence 1 had good safety, and the subjects had no adverse reactions.

[0125] From the results of sensitive skin application test, during the test period, no subjects had skin adverse reactions, indicating that essence 1-3 and essence 1 had good safety, were mild and non-irritating, and were suitable for sensitive skin population.

[0126] 2-3: Moisturizing property test:

[0127] Test substance: Essence 1-3, essence 1.

[0128] Subject selection: Healthy volunteers aged 21-44 years old, positive in lactic acid stinging test, voluntarily participate and sign the informed consent, can complete the test as required during the test period, randomly grouped, 30 subjects in each group.

[0129] Test method:

[0130] The subjects washed their forearms, sat quietly for 30 min in the set humidity environment, and selected a square test area with a side length of 3 cm on the left and right forearms of the subjects. The left arm was used as the test area of the test substance, and the symmetric area of the right arm was used as the blank control. The moisture content of each test site was detected using Corneometer CM825, repeated 3 times, and the average value was recorded.

[0131] The calculation formula of skin hydration rate is:

[0132] Hydration rate = (test detection value - blank control detection value) / blank control detection value x 100%

[0133] The average skin hydration rate results of the subjects after using the samples for 2 hours are shown in Table 7.

[0134] Table 7 Effect of the serum on skin hydration rate

[0135] Group Hydration rate / % Serum 1 43.72 Serum 2 41.53 Serum 3 42.35 Serum 1 33.42

[0136] From the data in Table 7, it can be seen that the serums 1-3 have a significant effect of improving the skin hydration rate. By comparing the results of serum 1 and serum 1, it can be seen that the serum prepared by the preparation method of the present application has a more significant effect on improving the skin hydration rate.

[0137] The above-described embodiments are part of the embodiments of the present application, rather than all the embodiments. The detailed description of the embodiments of the present application is not intended to limit the scope of the claimed application, but only represents selected embodiments of the present application. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative labor fall within the scope of protection of the present application.

Claims

1. A moisturizing antioxidant composition comprising an extract of Eupatorium odoratum, characterized in that, The composition is composed of Euphorbia macropodoides tree bark extract, annona glabra fruit extract, adenium obesum leaf cell extract, cornus officinalis fruit extract and PDRN with a mass ratio of 1:0.8-1:2-3:1-2:1-1.5; The PDRN is purchased from Regenesis (Shandong) Biotechnology Co., Ltd., and the model is RJMPDRN-850K; The Euphorbia macropodoides tree bark is purchased from Shanghai Qianfei Chemical Co., Ltd.; The annona glabra fruit extract is purchased from Shanghai Yanganfu Industry Co., Ltd.; The adenium obesum leaf cell extract is purchased from Shanghai Xinsengmeike Biological Co., Ltd.; The cornus officinalis fruit is purchased from Guangzhou Nuoyuan Biological Technology Co., Ltd.

2. The moisturizing antioxidant composition containing extracts of Euphorbia tirucalli according to claim 1, characterized in that, The composition is composed of Euphorbia macropodoides tree bark extract, annona glabra fruit extract, adenium obesum leaf cell extract, cornus officinalis fruit extract and PDRN with a mass ratio of 1:0.9:2.5:1.5:1.

3.

3. Use of the moisturizing and antioxidant composition containing Euphorbia macropodoides extract according to claim 1 or 2 in the preparation of cosmetics with moisturizing and antioxidant effects.

4. Use according to claim 3, characterized in that, The dosage form of the cosmetic is any one of cream and essence.

5. An essence having moisturizing and antioxidant effects, characterized by comprising the extract of claim 1. The essence contains 4-6wt% of the moisturizing and antioxidant composition containing Euphorbia macropodoides extract according to claim 1 or 2.

6. The serum with moisturizing and antioxidant efficacy according to claim 5, characterized in that, The essence further contains the following components by mass percentage: Dipotassium glycyrrhizate: 0.1-0.3wt%; Hydroxypropyl methyl cellulose: 0.5-1.5wt%; Deionized water is added to 100wt%.

7. The method for preparing the essence having the moisturizing and antioxidation efficacy according to claim 6, characterized in that, The method comprises the following steps: A1: uniformly mix 2 / 3 of the deionized water with Euphorbia macropodoides tree bark extract, annona glabra fruit extract, adenium obesum leaf cell extract, cornus officinalis fruit extract, dipotassium glycyrrhizate and hydroxypropyl methyl cellulose at 50-60℃, and cool to 2-8℃ to obtain a mixed solution A; A2: uniformly mix PDRN with the remaining deionized water at 2-8℃ to obtain a mixed solution B; A3: uniformly mix the mixed solution A and the mixed solution B at 2-8℃ to obtain an essence, seal and store at 2-8℃ in the dark.

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