Chinese herbal medicine composition, preparation method and application thereof in freckle-removing and whitening products

Abstract

The present invention discloses a Chinese herbal medicine composition, a preparation method and its application in freckle removal and whitening products. The Chinese herbal medicine composition comprises the following raw materials in parts by weight: 45-60 parts of honeysuckle / cornus officinalis fermentation product, 20-35 parts of bletilla striata extract, and 20-35 parts of zedoary turmeric extract; wherein, the honeysuckle / cornus officinalis fermentation product is obtained by activating bifidobacteria and inoculating them into a seed culture medium to obtain a seed liquid, and then transferring the seed liquid to a fermentation medium for cultivation to obtain a fermentation liquid, and finally filtering and freeze-drying the fermentation liquid; wherein, the seed culture medium contains cornus officinalis extract, and the fermentation medium contains honeysuckle extract and cornus officinalis extract. The Chinese herbal medicine composition of the present application has a synergistic effect between the various components, and has significant effects such as anti-oxidation, tyrosine inhibition, and repair of ultraviolet-aged skin.

Description

Technical Field

[0001] The present invention relates to the technical field of Chinese herbal medicines, in particular to a Chinese herbal medicine composition, a preparation method and application thereof in freckle-removing and whitening products. Background Art

[0002] As living standards improve, people are more conscious of their appearance than ever before. This awareness and ability to improve skin problems are also increasing, leading to a growing demand for skincare products and a growing emphasis on their safety and effectiveness. The formation of dark spots is mostly caused by the accumulation and deposition of melanin, a pigment produced by melanocytes in the human body and then transported to the surface of the skin, resulting in dark spots. Currently, there are two methods for removing dark spots: physical methods, which reduce melanin production by shielding against UV radiation; and active ingredients that inhibit tyrosinase activity.

[0003] Modern whitening and freckle-removing active ingredients are generally obtained through two ways: the first is to obtain extracts of biological agents such as animals and plants through extraction and other methods; the second is to obtain whitening active ingredients through chemical synthesis, such as: glycolic acid, kojic acid and its derivatives, gallic acid, arbutin, vitamin C and its derivatives, glutathione, cysteine, hesperidin, niacinamide, tranexamic acid, salicylic acid, retinoids, ellagic acid, linoleic acid, asiaticoside, and glycyrrhizin.

[0004] With the advancement of traditional Chinese medicine purification technology and the refinement of traditional Chinese medicine preparations, topical Chinese medicines have become unique in the beauty and skincare market due to their minimal toxicity, ease of use, and intuitive efficacy. Our team, combining years of experience in cosmetic formulation development with traditional Chinese herbal fermentation technology, has conducted research and development on fermentation product raw materials with skin-whitening and repairing properties. Through formulating these raw materials, we have developed a traditional Chinese herbal medicine combination with whitening, anti-freckle, antioxidant, and skin-repairing properties. Summary of the Invention

[0005] The purpose of the present invention is to overcome the deficiencies of the prior art and provide a Chinese herbal medicine composition, a preparation method and its application in freckle removal and whitening products to solve the problems raised in the above technical background.

[0006] To achieve the above object, the present invention is implemented through the following technical solutions:

[0007] In a first aspect, the present invention provides a Chinese herbal medicine composition comprising the following raw materials in parts by weight: 45-60 parts of honeysuckle / cornus officinalis fermentation product, 20-35 parts of bletilla striata extract, and 20-35 parts of zedoary turmeric extract;

[0008] The honeysuckle / cornus officinalis fermented product is obtained by activating bifidobacteria and inoculating them into a seed culture medium to obtain a seed liquid, then transferring the seed liquid into a fermentation culture medium to obtain a fermentation liquid, and finally filtering and freeze-drying the fermentation liquid; wherein the seed culture medium contains cornus officinalis extract, and the fermentation culture medium contains honeysuckle extract and cornus officinalis extract.

[0009] Preferably, the Chinese herbal medicine composition comprises the following raw materials in parts by weight: 50 parts of honeysuckle / cornus officinalis fermentation product, 30 parts of bletilla striata extract, and 30 parts of zedoary turmeric extract.

[0010] Preferably, the Bletilla striata extract and the Curcuma zedoaria extract are both obtained by water extraction followed by freeze-drying.

[0011] Preferably, the seed culture medium contains 2.5-4.0 g / L Cornus officinalis extract, 10.0 g / L beef extract powder, 5.0 g / L peptone, 3.0 g / L yeast extract powder, 5.0 g / L glucose, 1.0 g / L soluble starch, 5.0 g / L NaCl, 3.0 g / L NaAc, and 0.5 g / L L-cysteine ​​hydrochloride.

[0012] Further preferably, the seed culture medium contains 3.0 g / L Cornus officinalis extract, 10.0 g / L beef extract powder, 5.0 g / L peptone, 3.0 g / L yeast extract powder, 5.0 g / L glucose, 1.0 g / L soluble starch, 5.0 g / L NaCl, 3.0 g / L NaAc, and 0.5 g / L L-cysteine ​​hydrochloride.

[0013] Preferably, the fermentation medium contains 0.8-2.5 g / L honeysuckle extract, 1.2-3.0 g / L cornus officinalis extract, 10.0 g / L beef extract powder, 5.0 g / L peptone, 3.0 g / L yeast extract powder, 5.0 g / L glucose, 1.0 g / L soluble starch, 5.0 g / L NaCl, 3.0 g / L NaAc, and 0.5 g / L L-cysteine ​​hydrochloride.

[0014] Further preferably, the fermentation medium contains 1.5 g / L honeysuckle extract, 2.5 g / L cornus officinalis extract, 10.0 g / L beef extract powder, 5.0 g / L peptone, 3.0 g / L yeast extract powder, 5.0 g / L glucose, 1.0 g / L soluble starch, 5.0 g / L NaCl, 3.0 g / L NaAc, and 0.5 g / L L-cysteine ​​hydrochloride.

[0015] Preferably, the bifidobacterium is Bifidobacterium animalis CICC 6165.

[0016] In a second aspect, the present invention provides a method for preparing the above-mentioned Chinese herbal medicine composition, comprising the following steps:

[0017] S1. Activating Bifidobacterium and inoculating it into a seed culture medium containing Cornus officinalis extract and anaerobic culture at 37° C. to obtain a seed solution;

[0018] S2. transferring the seed solution to a fermentation medium containing Lonicera japonica extract and Cornus officinalis extract and culturing the mixture anaerobically at 37° C. to obtain a fermentation broth;

[0019] S3, filtering and freeze-drying the fermentation liquid to obtain a honeysuckle / cornus officinalis fermentation product;

[0020] S4, extracting with water, concentrating and freeze-drying to obtain Bletilla striata extract and Curcuma zedoaria extract respectively;

[0021] S5. Take 45-60 parts of honeysuckle / cornus officinalis fermentation product, 20-35 parts of bletilla striata extract, and 20-35 parts of zedoary turmeric extract, and mix them evenly to obtain a Chinese herbal medicine composition.

[0022] In a third aspect, the present invention provides an application of the Chinese herbal medicine composition in freckle removal and whitening products.

[0023] In a fourth aspect, the present invention provides the use of the Chinese herbal medicine composition in antioxidant skin repair products.

[0024] Compared with the prior art, the present invention has the following beneficial effects:

[0025] This application utilizes a culture medium containing honeysuckle extract and cornus officinalis extract as raw material, ferments it using animal Bifidobacterium CICC 6165, and compounding the fermented honeysuckle / cornus officinalis fermented product with bletilla striata extract and zedoaria extract to obtain a Chinese herbal medicine composition. The components in the Chinese herbal medicine composition act synergistically, have significant antioxidant, tyrosine inhibition and UV-aging skin repair effects, and have broad application prospects in cosmetics and medical beauty products. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] Figure 1 A bar graph showing the effect of the Chinese herbal medicine composition of the present invention on the tyrosinase inhibition rate;

[0027] Figure 2 A bar graph showing the effect of the Chinese herbal medicine composition of the present invention on DPPH free radical scavenging rate;

[0028] Figure 3 This is a bar graph showing the effect of the Chinese herbal medicine composition of the present invention on the SOD activity in the skin tissue of mice with skin photoaging model;

[0029] Figure 4The figure is a bar graph showing the effect of the Chinese herbal medicine composition of the present invention on the MDA content in the skin tissue of mice with skin photoaging model. DETAILED DESCRIPTION

[0030] The following describes the embodiments of the present invention through specific examples. Those skilled in the art can easily understand other advantages and effects of the present invention from the content disclosed in this specification. The present invention can also be implemented or applied through other different specific embodiments. The details in this specification can also be modified or changed based on different viewpoints and applications without departing from the spirit of the present invention. It should be noted that the following embodiments and features in the embodiments can be combined with each other unless they conflict.

[0031] In the present invention, honeysuckle, cornus officinalis, bletilla striata and zedoaria are all purchased from the market, and the quality requirements all meet the standards of the 2015 edition of the Pharmacopoeia.

[0032] In the present invention, the honeysuckle extract, cornus officinalis extract, bletilla striata extract and zedoary turmeric extract are all prepared by water extraction followed by concentration and freeze-drying; specifically:

[0033] Preparation of honeysuckle extract: crush the honeysuckle into 100 mesh particles, mix with 10 times its weight of water, and extract by ultrasonic in a 45°C water bath for 2 hours. Centrifuge and filter the extract, and set aside the filtrate; take the residue and continue to mix it with 10 times its weight of water, boil for 1.5 hours, filter, combine the two filtrates, concentrate and freeze-dry, which is the honeysuckle extract.

[0034] Preparation of Cornus officinalis extract: Grind Cornus officinalis into 100 mesh particles, mix with 10 times its weight of water, and extract by ultrasonic in a water bath at 45°C for 2 hours. Centrifuge and filter the extract, and set aside the filtrate. Take the residue and continue to mix it with 10 times its weight of water, boil for 1.5 hours, filter, combine the two filtrates, concentrate and freeze-dry to obtain the Cornus officinalis extract.

[0035] Preparation of Bletilla striata extract: Take Bletilla striata, crush it into 100 mesh particles, add 10 times the amount of water, decoct for 1 hour, filter, and set aside the filtrate; take the residue and continue to add 8 times the amount of water, decoct for 40 minutes, filter, and set aside the filtrate; take the residue and continue to add 8 times the amount of water, decoct for 40 minutes, filter, discard the residue, combine the filtrates three times and concentrate and freeze-dry to obtain the Bletilla striata extract.

[0036] Preparation of Curcuma zedoaria extract: Take Curcuma zedoaria, crush it into 100 mesh particles, add 10 times the amount of water, decoct for 1 hour, filter, and set aside the filtrate; take the residue and continue to add 8 times the amount of water, decoct for 40 minutes, filter, and set aside the filtrate; take the residue and continue to add 8 times the amount of water, decoct for 40 minutes, filter, discard the residue, combine the three filtrates and concentrate and freeze-dry to obtain the Curcuma zedoaria extract.

[0037] Honeysuckle / Cornus officinalis ferment:

[0038] (1) Strain

[0039] Bifidobacterium animalis CICC 6165 was purchased from China Industrial Microorganism Culture Collection Center.

[0040] (2) Preparation method:

[0041] S1. Use a sterile pipette to draw 0.5 ml of liquid culture medium from the freeze-dried tube to completely dissolve the freeze-dried bacterial powder of Bifidobacterium animalis CICC 6165. Transfer the dissolved bacterial suspension to a test tube containing 5 ml of liquid culture medium, mix thoroughly, and incubate in an anaerobic incubator at 37°C for 72 hours. Spread the activated liquid after 72 hours of incubation on the solid culture medium and incubate anaerobically at 37°C. After a single colony grows, take the single colony, dilute it with 0.5 mL of sterile water, and inoculate it into the seed culture medium at a volume ratio of 3 v / v%. Incubate anaerobically at 37°C for 24 hours to obtain the seed liquid.

[0042] Among them, the solid culture medium: 10.0g / L beef extract, 5.0g / L peptone, 3.0g / L yeast extract, 5.0g / L glucose, 1.0g / L soluble starch, 5.0g / L NaCl, 3.0g / L NaAc, 0.5g / L L-cysteine ​​hydrochloride, 15g / L agar, adjust the pH to 6.8, sterilize at 121℃ for 15min, and set aside. The liquid culture medium does not add agar. The seed culture medium contains 2.5~4.0g / L Cornus officinalis extract, 10.0g / L beef extract, 5.0g / L peptone, 3.0g / L yeast extract, 5.0g / L glucose, 1.0g / L soluble starch, 5.0g / L NaCl, 3.0g / L NaAc, 0.5g / L L-cysteine ​​hydrochloride, adjust the pH to 6.8, sterilize at 121℃ for 15min, and set aside.

[0043] S2. Transfer the seed liquid into a fermentation medium at a rate of 3 v / v%, and culture anaerobically at 37° C. for 48 h to prepare a fermentation broth; wherein the fermentation medium comprises 0.8-2.5 g / L honeysuckle extract, 1.2-3.0 g / L cornus officinalis extract, 10.0 g / L beef extract powder, 5.0 g / L peptone, 3.0 g / L yeast extract powder, 5.0 g / L glucose, 1.0 g / L soluble starch, 5.0 g / L NaCl, 3.0 g / L NaAc, and 0.5 g / L L-cysteine ​​hydrochloride; the pH is adjusted to 6.8, and the medium is sterilized at 121° C. for 15 min and set aside.

[0044] S3. The fermentation broth was inactivated by heating at 121°C for 15 minutes. 0.5 m / v% coconut shell activated carbon (10-18 mesh, Henan Jingke Water Treatment Materials Co., Ltd.) was added to the inactivated fermentation broth. After standing overnight, the supernatant was filtered, concentrated under reduced pressure, and freeze-dried to obtain a honeysuckle / cornus officinalis fermentation product. The 0.5 m / v% refers to the addition of 0.5 g of coconut shell activated carbon to 100 ml of fermentation broth.

[0045] Example 1

[0046] This embodiment provides a Chinese herbal medicine composition, the preparation method of which comprises the following steps:

[0047] S1. Bifidobacterium animalis CICC 6165 was activated and inoculated into a seed culture medium containing Cornus officinalis extract and anaerobically cultured at 37° C. to obtain a seed solution; Bifidobacterium animalis CICC 6165 was purchased from the China Industrial Microbiological Culture Collection Center.

[0048] S2. Transfer the seed liquid to a fermentation medium containing honeysuckle extract and cornus extract and culture it anaerobically at 37°C to obtain a fermentation liquid; wherein the seed culture medium contains 2.5 g / L cornus extract, 10.0 g / L beef extract powder, 5.0 g / L peptone, 3.0 g / L yeast extract powder, 5.0 g / L glucose, 1.0 g / L soluble starch, 5.0 g / L NaCl, 3.0 g / L NaAc, and 0.5 g / L L-cysteine ​​hydrochloride.

[0049] S3. Filter and freeze-dry the fermentation broth to obtain a honeysuckle / cornus officinalis fermented product; wherein the fermentation medium comprises 2.5 g / L honeysuckle extract, 1.2 g / L cornus officinalis extract, 10.0 g / L beef extract powder, 5.0 g / L peptone, 3.0 g / L yeast extract powder, 5.0 g / L glucose, 1.0 g / L soluble starch, 5.0 g / L NaCl, 3.0 g / L NaAc, and 0.5 g / L L-cysteine ​​hydrochloride. The specific preparation method of the honeysuckle / cornus officinalis fermented product is described above.

[0050] S4, extracting with water, concentrating and freeze-drying to obtain Bletilla striata extract and Curcuma zedoaria extract, respectively. The specific preparation methods are described above;

[0051] S5. Take 45 parts of honeysuckle / cornus officinalis fermentation product, 35 parts of bletilla striata extract, and 35 parts of zedoary turmeric extract, and mix them evenly to obtain a Chinese herbal medicine composition.

[0052] Example 2

[0053] This embodiment provides a Chinese herbal medicine composition, the preparation method of which comprises the following steps:

[0054] S1. Bifidobacterium animalis CICC 6165 was activated and inoculated into a seed culture medium containing Cornus officinalis extract and anaerobically cultured at 37° C. to obtain a seed solution; Bifidobacterium animalis CICC 6165 was purchased from the China Industrial Microbiological Culture Collection Center.

[0055] S2. Transfer the seed liquid to a fermentation medium containing honeysuckle extract and cornus extract and culture it anaerobically at 37°C to obtain a fermentation liquid; wherein the seed culture medium contains 4.0 g / L cornus extract, 10.0 g / L beef extract powder, 5.0 g / L peptone, 3.0 g / L yeast extract powder, 5.0 g / L glucose, 1.0 g / L soluble starch, 5.0 g / L NaCl, 3.0 g / L NaAc, and 0.5 g / L L-cysteine ​​hydrochloride.

[0056] S3. Filter and freeze-dry the fermentation broth to obtain a honeysuckle / cornus officinalis fermented product; wherein the fermentation medium comprises 0.8 g / L honeysuckle extract, 3.0 g / L cornus officinalis extract, 10.0 g / L beef extract powder, 5.0 g / L peptone, 3.0 g / L yeast extract powder, 5.0 g / L glucose, 1.0 g / L soluble starch, 5.0 g / L NaCl, 3.0 g / L NaAc, and 0.5 g / L L-cysteine ​​hydrochloride. The specific preparation method of the honeysuckle / cornus officinalis fermented product is described above.

[0057] S4, extracting with water, concentrating and freeze-drying to obtain Bletilla striata extract and Curcuma zedoaria extract, respectively. The specific preparation methods are described above;

[0058] S5. Take 60 parts of honeysuckle / cornus officinalis fermentation product, 20 parts of bletilla striata extract, and 20 parts of zedoary turmeric extract, and mix them evenly to obtain a Chinese herbal medicine composition.

[0059] Example 3

[0060] This embodiment provides a Chinese herbal medicine composition, the preparation method of which comprises the following steps:

[0061] S1. Bifidobacterium animalis CICC 6165 was activated and inoculated into a seed culture medium containing Cornus officinalis extract and anaerobically cultured at 37° C. to obtain a seed solution; Bifidobacterium animalis CICC 6165 was purchased from the China Industrial Microbiological Culture Collection Center.

[0062] S2. Transfer the seed liquid to a fermentation medium containing honeysuckle extract and cornus extract and culture it anaerobically at 37°C to obtain a fermentation liquid; wherein the seed culture medium contains 3.0 g / L cornus extract, 10.0 g / L beef extract powder, 5.0 g / L peptone, 3.0 g / L yeast extract powder, 5.0 g / L glucose, 1.0 g / L soluble starch, 5.0 g / L NaCl, 3.0 g / L NaAc, and 0.5 g / L L-cysteine ​​hydrochloride.

[0063] S3. Filter and freeze-dry the fermentation broth to obtain a honeysuckle / cornus officinalis fermented product; wherein the fermentation medium comprises 1.5 g / L honeysuckle extract, 2.5 g / L cornus officinalis extract, 10.0 g / L beef extract powder, 5.0 g / L peptone, 3.0 g / L yeast extract powder, 5.0 g / L glucose, 1.0 g / L soluble starch, 5.0 g / L NaCl, 3.0 g / L NaAc, and 0.5 g / L L-cysteine ​​hydrochloride. The specific preparation method of the honeysuckle / cornus officinalis fermented product is described above.

[0064] S4, extracting with water, concentrating and freeze-drying to obtain Bletilla striata extract and Curcuma zedoaria extract, respectively. The specific preparation methods are described above;

[0065] S5. Take 50 parts of honeysuckle / cornus officinalis fermentation product, 30 parts of bletilla striata extract, and 30 parts of zedoary turmeric extract, and mix them evenly to obtain a Chinese herbal medicine composition.

[0066] Comparative Example 1

[0067] This embodiment is similar to embodiment 3, except that the fermented product of honeysuckle and cornus officinalis is removed.

[0068] Comparative Example 2

[0069] This embodiment is similar to embodiment 3, except that the Bletilla striata extract is removed.

[0070] Comparative Example 3

[0071] This embodiment is similar to embodiment 3, except that the Curcuma zedoaria extract is removed.

[0072] Comparative Example 4

[0073] This example is similar to Example 3, using the same Chinese herbal medicine formula, except that the formula contains a different honeysuckle / cornus officinalis fermentation product and a different seed culture medium, comprising 10.0 g / L beef extract, 5.0 g / L peptone, 3.0 g / L yeast extract, 5.0 g / L glucose, 1.0 g / L soluble starch, 5.0 g / L NaCl, 3.0 g / L NaAc, and 0.5 g / L L-cysteine ​​hydrochloride. The fermentation medium is the same as that in Example 3.

[0074] Comparative Example 5

[0075] This example is similar to Example 3, using the same Chinese herbal medicine formula, except that the formula contains a different honeysuckle / cornus officinalis fermentation product and a different fermentation medium, comprising 1.5 g / L honeysuckle extract, 10.0 g / L beef extract powder, 5.0 g / L peptone, 3.0 g / L yeast extract powder, 5.0 g / L glucose, 1.0 g / L soluble starch, 5.0 g / L NaCl, 3.0 g / L NaAc, and 0.5 g / L L-cysteine ​​hydrochloride. The seed culture medium is the same as that in Example 3.

[0076] Comparative Example 6

[0077] This example is similar to Example 3, using the same Chinese herbal medicine formula, except that the formula contains a different honeysuckle / cornus officinalis fermentation product and a different fermentation medium, comprising 2.5 g / L cornus officinalis extract, 10.0 g / L beef extract powder, 5.0 g / L peptone, 3.0 g / L yeast extract powder, 5.0 g / L glucose, 1.0 g / L soluble starch, 5.0 g / L NaCl, 3.0 g / L NaAc, and 0.5 g / L L-cysteine ​​hydrochloride. The seed culture medium is the same as that in Example 3.

[0078] Comparative Example 7

[0079] This example is similar to Example 3, and the Chinese herbal medicine formula is the same, except that the formula contains different honeysuckle / cornus officinalis fermentation products, and the seed culture medium and fermentation medium used for the honeysuckle / cornus officinalis fermentation products are different. The seed culture medium contains 10.0 g / L beef extract, 5.0 g / L peptone, 3.0 g / L yeast extract, 5.0 g / L glucose, 1.0 g / L soluble starch, 5.0 g / L NaCl, 3.0 g / L NaAc, and 0.5 g / L L-cysteine ​​hydrochloride. The fermentation medium contains 10.0 g / L beef extract, 5.0 g / L peptone, 3.0 g / L yeast extract, 5.0 g / L glucose, 1.0 g / L soluble starch, 5.0 g / L NaCl, 3.0 g / L NaAc, and 0.5 g / L L-cysteine ​​hydrochloride.

[0080] Performance test of Chinese herbal medicine composition:

[0081] 1. Tyrosinase inhibition test

[0082] 1.1 Reagents: Disodium hydrogen phosphate-citrate buffer (pH 6.8): 154.5 mL of 0.2 mol / L Na2HPO4·12H2O, add 45.5 mL of 0.1 mol / L citric acid monohydrate to make 200 mL of disodium hydrogen phosphate-citrate buffer; use the disodium hydrogen phosphate-citrate buffer (pH 6.8) to prepare 100 U / mL tyrosinase solution and 1 mg / mL levodopa solution.

[0083] 1.2 Sample Solutions: The herbal compositions obtained in Examples 1-3 and Comparative Examples 1-7 were dissolved in a pH 6.8 sodium hydrogen phosphate-citric acid buffer solution and diluted to a corresponding sample concentration of 0.2 wt% (mass percentage concentration). A solution containing 0.09% of the honeysuckle / cornus officinalis fermented product was prepared using a pH 6.8 sodium hydrogen phosphate-citric acid buffer solution. A solution containing 0.05% of the bletilla striata extract was prepared using a pH 6.8 sodium hydrogen phosphate-citric acid buffer solution. A solution containing 0.05% of the zedoary turmeric extract was prepared using a pH 6.8 sodium hydrogen phosphate-citric acid buffer solution.

[0084] 1.3 A tyrosinase catalytic reaction system was constructed to evaluate the inhibitory effects of the Chinese herbal medicine compositions obtained in Examples 1 to 3 and Comparative Examples 1-7, the fermented products of honeysuckle / cornus officinalis, the extract of bletilla striata, and the extract of zedoaria on tyrosinase. Referring to the tyrosinase catalytic reaction system (Table 1), four reaction tubes A1, A2, A3, and A4 were divided for sample addition. The sample solution, pH 6.8 disodium hydrogen phosphate-citrate buffer solution, and tyrosinase solution were respectively drawn into the reaction tubes, shaken, and incubated in a 37°C water bath for 10 minutes. After taking out, levodopa solution was added, and after reacting for 5 minutes, the absorbance values ​​of A1, A2, A3, and A4 were measured at 475nm. Each sample was repeated 3 times. The tyrosinase inhibition rate was calculated according to the following formula. The results are shown in Tables 2 and Figure 1 shown.

[0085] Tyrosinase inhibition rate (%) = [1-(A3-A4) / (A1-A2)] × 100%, where A1, A2, A3, and A4 are the absorbance values ​​at 475 nm after the reaction in the corresponding reaction tubes.

[0086] Table 1

[0087]

[0088] 2. DPPH free radical scavenging test

[0089] 2.1 Sample Solution: Dissolve the herbal compositions obtained in Examples 1-3 and Comparative Examples 1-7 in distilled water and dilute to a sample concentration of 0.2 wt% (mass percentage). Prepare a solution containing 0.09% of the honeysuckle / cornus officinalis fermented product with distilled water, a solution containing 0.05% of the bletilla striata extract with distilled water, and a solution containing 0.05% of the zedoary turmeric extract with distilled water.

[0090] 2.2 Take 1 mL of sample solution, add 1 mL of 0.2 mmol / L DPPH ethanol solution, mix well and react in the dark at room temperature for 30 minutes, then use a microplate reader to measure the absorbance of the sample at 517 nm; the background group is a mixture of 1 mL of sample solution and 1 mL of anhydrous ethanol, and the blank control group is a mixture of 1 mL of distilled water and 1 mL of DPPH anhydrous ethanol solution. The DPPH free radical scavenging rate is calculated according to the formula, and the results are shown in Table 2 and Figure 2 The calculation formula is: Q=[1-(A1-A2) / A0]×100%,

[0091] Where:

[0092] Q: DPPH free radical scavenging rate,%;

[0093] A1: absorbance value of sample group;

[0094] A2: absorbance value of background group;

[0095] A0: absorbance of blank control group.

[0096] Table 2

[0097]

[0098] From Table 2, Figure 1 and Figure 2 It can be seen that the tyrosinase inhibition rate and DPPH free radical scavenging rate of the herbal compositions provided in Examples 1 to 3 are significantly higher than those of the herbal compositions, honeysuckle / cornus fermentation product, bletilla striata extract, and zedoary extract provided in Comparative Examples 1 to 7, indicating that the herbal compositions provided in the examples of the present application have better antioxidant capacity and stronger tyrosinase inhibition rate; indicating that the honeysuckle / cornus fermentation product, bletilla striata extract, and zedoary extract in the herbal composition have a synergistic effect and have significant whitening and antioxidant effects.

[0099] 3. The effect of Chinese herbal medicine combination on repairing skin photoaging

[0100] 3.1 Animal grouping and drug administration

[0101] 100 female KM mice were purchased from Beijing Huafukang Biotechnology Co., Ltd.; after normal feeding for one week, they were randomly divided into 10 groups, namely a normal group, a model group, and 8 drug-treated groups, with 10 mice in each group. The drug-treated groups were treated with the corresponding Chinese medicine compositions obtained in Example 3 and Comparative Examples 1-7, and the Chinese medicine compositions were diluted with physiological saline to a 0.2% solution.

[0102] Normal group: During the experimental period, the mice were depilated with a shaver on the center of their backs every day, with a depilatory area of ​​2 cm × 3 cm. No UV radiation and no drug treatment were performed.

[0103] Model group: During the experimental period, the mice were shaved with a shaver on the center of their backs every day, with a 2 cm × 3 cm hair removal area, and were exposed to UV radiation and no drug treatment;

[0104] Dosage group: During the experimental period, the center of the back of the mice was depilated with a shaver every day, with the depilated area of ​​2 cm × 3 cm. The mice were treated with the corresponding traditional Chinese medicine composition obtained in Example 3 and Comparative Examples 1-7 (2 mg each time) 1 hour before UV irradiation.

[0105] 2. Construction of skin photoaging model mice

[0106] The mice were shaved in the center of their backs, with a 2cm x 3cm area. Three UVA and five UVB lamps were suspended 30 cm above the exposed skin on the rats' backs, spaced 5 cm apart. The experiment lasted for 10 weeks. The combined UVA and UVB radiation doses were set at a MED (100 mJ / cm) for the first week. 2 ) for irradiation, 4 times a week (Monday, Wednesday, Friday, Sunday), and the irradiation dose will increase by one MED each week compared with the previous week until 4 MEDs in the fourth week. The irradiation dose will be maintained at 4 MEDs in the fourth week and thereafter.

[0107] The normal group underwent daily hair removal, no UV radiation, and no drug treatment; the model group underwent daily hair removal, and was subjected to UV radiation on Mondays, Wednesdays, Fridays, and Sundays (the irradiation dose was as described above), and was not subjected to drug treatment; the drug treatment group underwent daily hair removal and UV radiation (the irradiation dose was as described above), and was treated with the corresponding traditional Chinese medicine composition obtained in Example 3 and Comparative Examples 1-7 (2 mg each time) 1 hour before UV radiation;

[0108] Test indicators

[0109] After the experiment, the mice in each group were killed by cervical dislocation. The skin tissues from the backs of the mice in each group were collected. After the subcutaneous fat was removed from the skin tissues, they were rinsed three times with pre-cooled physiological saline and dried with filter paper. The tissues were weighed and minced under ice bath conditions. Nine times the amount of pre-cooled 0.9% physiological saline was added and the tissues were homogenized and centrifuged under ice bath conditions. The SOD activity and MDA content in the tissues were detected using a kit (Nanjing Jiancheng Bioengineering Institute). The results are shown in Table 3. Figure 3 and Figure 4 shown.

[0110] Table 3

[0111]

[0112] From Table 3, Figure 3 and Figure 4 As can be seen, compared with the normal group, the SOD activity in the skin tissue of the model mice was significantly reduced, while the MDA content was significantly increased, indicating that the model was successfully established and the mouse skin had undergone photoaging. Compared with Comparative Examples 1-7, the SOD activity in the skin tissue of the mice in Example 3 was significantly increased, and its MDA content was significantly reduced; this shows that the honeysuckle / cornus officinalis fermentation product, Bletilla striata extract, and Curcuma zedoaria extract can synergistically repair SOD activity and reduce MDA content in the skin tissue of photoaged rats, showing an effect on repairing UV-induced skin aging.

[0113] This shows that the Chinese herbal medicine composition provided in this application can not only whiten and remove spots, and resist oxidation, but also repair photo-aged skin after ultraviolet irradiation.

[0114] The above-described embodiments merely represent specific implementations of the present invention. While the descriptions are relatively specific and detailed, they should not be construed as limiting the scope of the present invention. It should be noted that a person skilled in the art would be able to make numerous variations and improvements without departing from the spirit of the present invention, and all such variations and improvements fall within the scope of protection of the present invention.

Claims

1. A Chinese herbal medicine composition, characterized in that: The invention is composed of the following raw materials in parts by weight: 45-60 parts of honeysuckle flower / cornus officinalis fermented material, 20-35 parts of bletilla striata extract, and 20-35 parts of zedoary turmeric extract; The honeysuckle / cornus officinalis fermented product is obtained by activating bifidobacteria and inoculating them into a seed culture medium to obtain a seed liquid, then transferring the seed liquid into a fermentation culture medium to obtain a fermentation liquid, and finally filtering and freeze-drying the fermentation liquid; wherein, the seed culture medium contains 2.5~4.0g / L cornus officinalis extract, and the fermentation culture medium contains 0.8~2.5g / L honeysuckle extract and 1.2~3.0g / L cornus officinalis extract; the bifidobacterium is selected from animal Bifidobacterium CICC6165.

2. A Chinese herbal medicine composition according to claim 1, characterized in that: The preparation method is composed of the following raw materials in parts by weight: 50 parts of honeysuckle flower / cornus officinalis fermentation product, 30 parts of bletilla striata extract and 30 parts of zedoary turmeric extract.

3. A Chinese herbal medicine composition according to claim 1, characterized in that: The bletilla striata extract and the zedoary turmeric extract are both obtained by water extraction and then freeze-drying.

4. A Chinese herbal medicine composition according to claim 1, characterized in that: The seed culture medium comprises 2.5-4.0 g / L cornus officinalis extract, 10.0 g / L beef extract powder, 5.0 g / L peptone, 3.0 g / L yeast extract powder, 5.0 g / L glucose, 1.0 g / L soluble starch, 5.0 g / L NaCl, 3.0 g / L NaAc, and 0.5 g / L L-cysteine ​​hydrochloride.

5. A Chinese herbal medicine composition according to claim 4, characterized in that: The fermentation medium comprises 0.8-2.5 g / L honeysuckle extract, 1.2-3.0 g / L cornus officinalis extract, 10.0 g / L beef extract powder, 5.0 g / L peptone, 3.0 g / L yeast extract powder, 5.0 g / L glucose, 1.0 g / L soluble starch, 5.0 g / L NaCl, 3.0 g / L NaAc, and 0.5 g / L L-cysteine ​​hydrochloride.

6. A Chinese herbal medicine composition according to claim 5, characterized in that: The seed culture medium comprises 3.0 g / L cornus officinalis extract, 10.0 g / L beef extract powder, 5.0 g / L peptone, 3.0 g / L yeast extract powder, 5.0 g / L glucose, 1.0 g / L soluble starch, 5.0 g / L NaCl, 3.0 g / L NaAc, and 0.5 g / L L-cysteine ​​hydrochloride; The fermentation medium contains 1.5 g / L honeysuckle extract, 2.5 g / L cornus officinalis extract, 10.0 g / L beef extract powder, 5.0 g / L peptone, 3.0 g / L yeast extract powder, 5.0 g / L glucose, 1.0 g / L soluble starch, 5.0 g / L NaCl, 3.0 g / L NaAc, and 0.5 g / L L-cysteine ​​hydrochloride.

7. The method for preparing the Chinese herbal medicine composition according to any one of claims 1 to 6, characterized in that: The following steps are involved: S1. Activating Bifidobacterium and inoculating it into a seed culture medium containing Cornus officinalis extract and anaerobic culture at 37° C. to obtain a seed solution; S2. transferring the seed solution to a fermentation medium containing Lonicera japonica extract and Cornus officinalis extract and culturing the mixture anaerobically at 37° C. to obtain a fermentation broth; S3, filtering and freeze-drying the fermentation liquid to obtain a honeysuckle / cornus officinalis fermentation product; S4, extracting with water, concentrating and freeze-drying to obtain Bletilla striata extract and Curcuma zedoaria extract respectively; S5. Take 45-60 parts of honeysuckle / cornus officinalis fermentation product, 20-35 parts of bletilla striata extract, and 20-35 parts of zedoary turmeric extract, and mix them evenly to obtain a Chinese herbal medicine composition.

8. Use of the Chinese herbal medicine composition according to any one of claims 1 to 6 in freckle-removing and whitening products.

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