106 results about "Hair cell" patented technology

The disclosure provides polynucleotides containing regions of the Myosin 15 (Myo15) promoter, as well as vectors containing the same, that can be used to promote expression of a transgene specifically in hair cells. The polynucleotides described herein may be operably linked to a transgene, such as a transgene encoding a therapeutic protein, so as to promote hair cell-specific expression of the transgene. The polynucleotides described herein may be operably linked to a therapeutic transgene and used for the treatment of subjects having or at risk of developing hearing loss or vestibular dysfunction.

The invention discloses an Lhfpl5 gene enhancer and application thereof, and belongs to the field of gene engineering. According to the invention, the Lhfpl5 gene enhancer is found, the enhancer can obviously enhance the expression of the Lhfpl5 gene or other genes in inner ear internal and external hair cells, and the gene enhancer can be used for gene therapy of deafness by utilizing the capability of specifically enhancing the gene expression in the inner ear internal and external hair cells; the recombinant DNA containing the Lhfpl5 gene enhancer, or a carrier and other biological materials are prepared through integration, gene expression regulation and control research is carried out, and the application prospect is good.

One end of an input optical fiber is connected with a light source emitting end, and the other end of the input optical fiber sequentially penetrates through a sensor base and an artificial cilia cantilever beam to be fixedly connected with an exposed optical fiber; the other end of the exposed fiber is fixedly connected with the output fiber; and the other end of the output optical fiber penetrates through the artificial cilium cantilever beam and is in communication connection with the light source receiving end. The sensor provided by the invention can monitor various parameters such as the flow velocity, the flow velocity direction, the salinity and the like at the same time, and can meet the multi-dimensional monitoring requirements in a complex marine environment. Meanwhile, by simulating the structure of lateral hair cells of fishes and adopting a bendable artificial cilia cantilever beam as a core sensing element, the speed and direction of water flow can be accurately detected. In addition, the sensor is in direct contact with the fluid through the exposed optical fiber part, and the salinity of the fluid is detected by utilizing the difference of refractive indexes of different fluids.

A method for operating a hearing device that include a microphone and a receiver that are signal-connected by way of a signal processing unit. A signal component that includes a specific frequency is selected from an input signal. A further signal at a further specific frequency is provided, and an output signal is created on a basis of the signal component and the further signal. The output signal is output by the receiver to create a sound. The further signal is configured such that first time intervals and second time intervals arise, with hair cells associated with a specific frequency assigned region of a basilar membrane of a user being excited on account of an interference during the first time intervals and there being no excitation of the hair cells during the second time intervals. There is also described a corresponding hearing device.

The present invention discloses an Ush1c gene enhancer and its application, belonging to the field of genetic engineering, and the nucleotide sequence of the enhancer is shown in SEQ ID No. 1. The mouse embryo microinjection system is used to confirm that the sequence is an enhancer, which can significantly enhance the transcription activity of the EGFP protein gene in the inner ear. Compared with other sequences of similar size, the enhancer sequence fragment can drive the reporter gene or Ush1c to express significantly in the inner ear, and specifically express it in inner and outer hair cells, and is suitable for recombinant DNA, vectors or adenovirus and other biological materials, and is used to promote the transcription of EGFP gene or other genes in the inner ear.

To provide novel therapies targeting hair cells for the treatment of sensorineural hearing loss or vestibular dysfunction.SOLUTION: The disclosure provides polynucleotides containing regions of the Myosin 15 (Myo15) promoter, as well as vectors containing the same, that can be used to promote expression of a transgene specifically in hair cells. The polynucleotides described herein may be operably linked to a transgene, such as a transgene encoding a therapeutic protein, so as to promote hair cell-specific expression of the transgene. The polynucleotides described herein may be operably linked to a therapeutic transgene and used for the treatment of subjects having or at risk of developing hearing loss or vestibular dysfunction.SELECTED DRAWING: Figure 1

The invention discloses application of a carrier carrying Pou4f3 genetic information in preparation of a medicine for treating DFNA15 deafness, and belongs to the technical field of gene therapy, the DFNA15 deafness is that POU4F3 is mutated, and the POU4F3 is mutated into POU4F3c. 337Cgt; the medicine for treating the T-type mutation DFNA15 deafness carries a carrier with Pou4f3 genetic information, the AAV carrier is a combination of AAV-Anc80L65 and a Myo15 promoter, and the AAV carrier is delivered to an inner ear through round window membrane injection, targets outer hair cells and compensates for insufficient haplodose of POU4F3. The invention verifies that AAV delivers POU4F3 gene to compensate c.337Cgt for the first time; deafness caused by dominant genetic mutation of DFNA15 can be corrected due to insufficient haplodose caused by T (p.Q113 *) mutation, the optimized AAV vector design breaks through the problem of insufficient hair cell targeting of a traditional vector, the treatment efficiency is remarkably improved, meanwhile, a P60-P180 dynamic monitoring system is established, it is proved that the curative effect is lasting, no side effect exists, and a reliable basis is provided for clinical transformation.

The invention discloses a method for promoting maturation of cochlea organs in a GELMA-MXene matrix based on electroacoustic stimulation, and belongs to the field of cochlea organ culture for hair cell regeneration. The preparation method of the cochlea organ culture component comprises the following steps: preparing GelMA; sequentially adding LiF, MAX, pure water and acid into the reaction container; stirring the mixture until the reaction is completed, centrifuging the mixture, and removing supernate and MXene to obtain Ti3C2Tx; dissolving GelMA by using a light curing agent, and adding Ti < 3 > C < 2 > T < x >; and irradiating with blue light to obtain the cochlea organ culture component. Using the cochlea organ culture component to culture inner ear stem cells, and adding a differential medium; and after the differentiation is completed, applying low-frequency electroacoustic stimulation to the differentiated organoid. The advantages of a culture support and electroacoustic stimulation are combined, and differentiation and maturation of cochlea organs are more effectively promoted.

The invention relates to a dietary therapy formula for improving microcirculation of inner ears and a preparation method. Components with homology of medicine and food are selected, the black rice, the blueberries and the purple cabbages are rich in anthocyanin and beneficial to removing free radicals in cochlea and inhibiting hair cell apoptosis, the walnut kernels and the Chinese wolfberry fruits can improve blood vessel elasticity and protect auditory neurons, and the radix salviae miltiorrhizae, the hawthorn fruits and the radix puerariae can increase blood flow of inner ears, promote nutrition delivery, reduce blood viscosity and reduce vasospasm, so that the health-care food is suitable for being eaten by people. The whole component is low in sugar content, suitable for diabetics and capable of expanding the crowd. Effective components are processed into non-medicinal products, food therapy is beneficial to improving microcirculation of inner ears, relieving ear symptoms such as tinnitus and the like and delaying hearing loss, and a user can eat the food for a long time without psychological burden of medication.

The invention relates to the technical field of biological medicines, in particular to an adeno-associated virus AAV-IETP2 with a function of crossing a blood lost barrier and application of the adeno-associated virus AAV-IETP2. The adeno-associated virus AAV-IETP2 is prepared by inserting a nucleotide IETP2 oligopeptide capable of coding a blood lost barrier penetrating factor into an N end of a protein coding sequence of the adeno-associated virus VP2, and the nucleotide IETP2 oligopeptide has at least 80% of homology with a nucleotide sequence as shown in SEQ ID NO.1. The AAV-IETP2 disclosed by the invention can be administered through intravenous administration, and can penetrate through a blood labyrinth barrier to reach inner ear hair cells.

This utility model discloses a low-noise centrifugal pump for petrochemical applications, comprising a centrifugal pump body, an impeller movably mounted inside the pump body with an impeller shaft mounted on it, an inlet pipe at the front of the pump body, a drain pipe at the top of the pump body, and a low-noise mechanism wrapped around the outside of the pump body. The low-noise mechanism consists of noise reduction covers A, B, C, and D. Noise reduction cover B is fixedly mounted at one end of noise reduction cover A, and noise reduction cover D is fixedly mounted at one end of noise reduction cover C. This low-noise centrifugal pump for petrochemical applications features modularly designed noise reduction covers that can be quickly installed using a mounting bracket without complex tools or specialized skills, reducing the noise level generated by the centrifugal pump and preventing damage to the inner ear hair cells of factory workers, thus avoiding irreversible hearing loss. The rock wool itself has a porous fiber structure, primarily absorbing mid-to-high frequency noise.

The invention relates to the technical field of noise deafness susceptibility grading, in particular to a noise deafness susceptibility grading method and system based on a multi-band hearing threshold, and the method comprises the steps: presetting measurement frequency points, generating a measurement frequency point set according to a sequence from high frequency to low frequency, and completing M times of independent measurement for each frequency point; recording a tested subjective response timestamp, and generating a confidence value label; an otoacoustic emission signal in a tested ear canal is collected in real time, a time domain signal with the highest confidence coefficient of a single frequency point is extracted, spectrum features are calculated, and otoacoustic emission joint parameters are constructed. According to the method, the time sequence diffusion rule from high frequency to low and medium frequency hair cell damage can be comprehensively captured, the identification accuracy of potential auditory impairment is remarkably improved, the propagation mechanism from high frequency damage to intermediate frequency can be simulated by constructing the physically constrained spectrum migration operator, and otoacoustic emission time domain-frequency domain joint parameters and physiological indexes are combined, so that the identification accuracy of the potential auditory impairment is improved. And individualized and dynamic injury risk prediction is realized.

The invention belongs to the technical field of transgenic and biomedical research tools, and particularly relates to a method for constructing a transgenic fish system based on a Tol2 transposon system, which comprises the following steps: constructing a myo6b-lhfpl5b-DsRed plasmid by using myo6b as a promoter, lhfpl5b as a target gene and DsRed as a red fluorescent protein tag through a microinjection method, and constructing a Tol2 transposon system-based transgenic fish system. Co-injecting the plasmid containing the Tol2 transposase mRNA and the Tol2 transposase mRNA into a fertilized egg of an AB zebra fish strain, and screening generation by generation to obtain a transgenic fish strain with stable inheritance MET channel gene lhfpl5b marked hair cells. The spatio-temporal dynamic visualization of the living whole fish to the lhfpl5b is realized, the deafness-causing molecular pathway is clarified, and an in-vivo platform is provided for drug screening and intervention strategies.

The invention discloses an HSP70-targeting chimeric antigen receptor-macrophage and application thereof in treatment of noise-induced hearing loss, an extracellular domain of the HSP70-targeting chimeric antigen receptor comprises an HSP70 specific binding fragment, an intracellular domain comprises an IL-4 signal channel functional element, a signal switch type CAR structure of the HSP70 is formed, and the macrophage can be used for treating noise-induced hearing loss, so that the HSP70-targeting chimeric antigen receptor-macrophage can be used for treating noise-induced hearing loss. The HSP70-targeted chimeric antigen receptor-macrophage can be used for preparing the HSP70-targeted chimeric antigen receptor-macrophage, is used for mediating polarization of the macrophage to an M2 type and exerting an anti-inflammatory function, is proved to be capable of specifically responding to an inner ear inflammation microenvironment, switching an anti-inflammatory phenotype and protecting hair cells and auditory nerves, can be used for treating noise-induced hearing loss, and can be used for preparing the HSP70-targeted chimeric antigen receptor-macrophage. The limitation that traditional hearing-aid equipment supports treatment on symptoms is broken through, and the hearing-aid device has a remarkable application prospect.

The invention discloses a bionic acoustic function integrated superstructure processor and a use method. The device comprises a plurality of bionic hair cell discrete resonance units and a snail-shaped sound channel connected with the bionic hair cell discrete resonance units. Each bionic hair cell discrete resonance unit is composed of a neck tube and a back cavity, and the neck tube is contracted in the back cavity so as to reduce the occupied structure volume. The method comprises the following steps: determining the number of bionic hair cell discrete resonance units and the appearance and the size of a cochlear sound channel; dividing a frequency band into a plurality of sub-bands, jointly adjusting size parameters of a neck tube and a dorsal cavity in each bionic hair cell discrete resonance unit and an arrangement sequence and an arrangement distance of the neck tube and the dorsal cavity on a cochlear sound channel, and iteratively designing a superstructure processor; the combination of the multi-channel measurement results results in an expected response result. The invention enables the development of acoustic devices with advanced hearing functions, which may replace human higher-order acoustic information processing in scenarios dependent on human subjective judgments and experiences.

The embodiment of the invention provides a cell guiding device, and the device comprises a receiving coil which is used for receiving a radio frequency signal transmitted by an in-vitro energy supply device; the stimulator is electrically connected with the receiving coil and is used for generating an electrical stimulation pulse according to the radio frequency signal; the electrode array is used for being arranged in the cochlea and electrically connected with the stimulator; the electrode array is used for applying the electrical stimulation pulse to hair cells in the cochlea so as to stimulate the hair cells to proliferate and differentiate. According to the technical scheme provided by the embodiment of the invention, the electrical stimulation pulse is generated according to the in-vitro radio frequency signal and is applied to the hair cells in the cochlea, so that accurate and efficient electrical stimulation on the hair cells can be realized. Meanwhile, the implanted design is adopted to directly act on the hair cells in the cochlea, so that the trauma to the body can be reduced, and the safety and comfort of treatment are improved.

The present disclosure relates to myosin 15 promoters and uses thereof. The present disclosure provides polynucleotides containing a region of a myosin 15 (Myo15) promoter, which can be used to promote specific expression of a transgene in hair cells, as well as vectors containing the polynucleotides. The polynucleotides described herein can be operably linked to a transgene, such as a transgene encoding a therapeutic protein, in order to promote hair cell-specific expression of the transgene. The polynucleotides described herein can be operably linked to a therapeutic transgene and used to treat a subject having or at risk of developing hearing loss or vestibular dysfunction.

The disclosure provides polynucleotides containing regions of the Myosin 15 (Myo15) promoter, as well as vectors containing the same, that can be used to promote expression of a transgene specifically in hair cells. The polynucleotides described herein may be operably linked to a transgene, such as a transgene encoding a therapeutic protein, so as to promote hair cell-specific expression of the transgene. The polynucleotides described herein may be operably linked to a therapeutic transgene and used for the treatment of subjects having or at risk of developing hearing loss or vestibular dysfunction.

The invention relates to a method (26) for operating a hearing aid (2) comprising a microphone (6) and a receiver (12) which are connected by means of a signal processing unit (10). An input signal (30) is provided, and a signal component (36) of the input signal (30) having a specific frequency (40) is selected. Another signal (44) with a further specific frequency (46) is provided, and an output signal (50) is generated based on the signal component (36) and the further signal (44). The output signal (50) is output via the receiver (12), thus producing a sound.The further signal (44) is configured such that first time intervals (54) and second time intervals (56) result, wherein during the first time intervals (54) hair cells located in a region (22) of a user's basilar membrane (20) associated with a specific frequency (40) are stimulated due to interference, and wherein during the second time intervals (56) the hair cells are not stimulated. The invention further relates to a hearing aid (2).

The invention discloses a Pcdh15 gene enhancer sequence and application thereof, and belongs to the field of gene engineering, and the nucleotide sequence of the enhancer is shown as SEQ ID No.1. A mouse embryo microinjection system is used for verifying that the sequence is an enhancer, and the transcriptional activity of EGFP protein genes in hair cells can be remarkably enhanced in inner ears. Compared with other sequences with similar sizes, the enhancer sequence fragment can drive the expression ability of a reporter gene or a Pcdh15 gene to be obviously enhanced in inner ear hair cells, is specifically expressed in inner and outer hair cells, is suitable for biological materials such as recombinant DNA (Deoxyribonucleic Acid), vectors or adenoviruses and the like, and has a wide application prospect. The method is used for promoting transcription of EGFP genes or other genes in internal and external hair cells of inner ears.

The present disclosure describes systems and methods to evaluate auditory efferent function by exposing a patient to short-duration acoustic click stimuli to generate a click-evoked otoacoustic emission (CEOAE) occurring in each ear of the patient, and in response to exposing the patient to click stimuli, concurrently sampling outer hair cell activity (OHC) and medial olivocochlear reflex (MOCR; efferents) in each ear of the patient, monitoring the middle ear muscle reflex (MEMR) in each ear of the patient, and measuring a change in cochlear activity in the patient based on the CEOAE, MOOR, and MEMR of each ear of the patient.

The present disclosure describes gene therapy systems, and related methods, useful for treating and / or preventing deafness caused by genetic mutation of the TMPRSS3 gene or the LOXHD1 gene. The compositions and methods disclosed herein use adeno-associated viral (AAV) vector gene delivery of TRMPSS3 or LOXHD1 into the inner ear to restore activity of the TMPRSS3 gene or the LOXHD1 gene, respectively, promote hair cell survival and restore hearing in patients suffering from hearing loss. As disclosed herein, the systems and methods may utilize a combination of gene therapy (e.g., molecular therapeutics) for hearing loss caused by a genetic mutation together with implantation of a cochlear implant.

The invention relates to an application of glycyrrhizic acid-curcumin micelles in preparation of a medicine for treating deafness caused by gentamicin, and in the application, the glycyrrhizic acid-curcumin micelles are prepared from the following raw material medicines in percentage by weight: 66-89% of ammonium glycyrrhizinate and 11-34% of curcumin. According to the application, the glycyrrhizic acid-curcumin micelle can obviously improve hearing function impairment caused by drugs and obviously increase the fluorescent staining area of hair cells, and the effect of treating deafness caused by gentamicin is obvious.

The application discloses a kind of based on bionic lateral line's curved optical fiber multi-parameter sensor, one end of input optical fiber is connected with light source emission end, the other end is sequentially penetrated through the sensor base and artificial cilium cantilever beam and bare optical fiber fixed connection;The other end of bare optical fiber is fixedly connected with output optical fiber;The other end of output optical fiber is penetrated through artificial cilium cantilever beam and is connected with the light source receiving end communication connection.The sensor of the application can monitor the size of flow velocity, flow velocity direction, salinity and other multiple parameters simultaneously, and can meet the multidimensional monitoring demand under complex marine environment.Meanwhile, by imitating the structure of fish lateral line hair cell, using flexible artificial cilium cantilever beam as core perception element, the accurate detection of water flow velocity size and direction can be realized.In addition, the sensor is directly contacted with fluid through bare optical fiber part, and the detection of fluid salinity is realized by using the difference of different fluid refractive index.

The invention relates to application of ginkgo leaf exosomes in preparation of a medicine for treating hearing loss, and belongs to the field of medical technologies and biomedical materials. According to the application of the ginkgo leaf exosome in preparing the medicine for treating the hearing loss, the ginkgo leaf exosome can scavenge free radicals, so that inner ear hair cell apoptosis is inhibited, the inner ear active oxygen content is reduced, and the effect of treating the hearing loss is achieved. Therefore, the technical problems that in the prior art, the bioavailability of the ginkgo leaf extract is low, and the metabolism rate of active ingredients in the body is high are solved.

The disclosure provides polynucleotides containing regions of the Myosin 15 (Myo15) promoter, as well as vectors containing the same, that can be used to promote expression of a transgene specifically in hair cells. The polynucleotides described herein may be operably linked to a transgene, such as a transgene encoding a therapeutic protein, so as to promote hair cell-specific expression of the transgene. The polynucleotides described herein may be operably linked to a therapeutic transgene and used for the treatment of subjects having or at risk of developing hearing loss or vestibular dysfunction.

The invention belongs to the technical field of medicine, and particularly provides application of clotrimazole in preparation of a medicine for preventing and relieving cisplatin toxicity, the clotrimazole is an artificially synthesized imidazole antifungal compound, and the purity of the clotrimazole is 99.97% or above; the protective effect of clotrimazole in hair cells and hair cell lines of cochlea basilar membranes cultured in vitro is verified through HEI-OC1 cell culture and C57 mouse basilar membrane culture, and the in-vivo effect of clotrimazole is verified through tympanic injection of C57 mice; besides, the research test of clotrimazole for mouse cis-platinum ear toxicity shows that hearing thresholds of all frequencies after cis-platinum treatment are obviously increased, ABR threshold offset caused by cis-platinum is reduced by injection of clotrimazole into tympanic chambers, hair cell loss of all gynaecology is greatly improved due to the protection effect of clotrimazole, and the cis-platinum ear toxicity of mice is improved. Finally, the clotrimazole is proved to have a remarkable effect on prevention or treatment of cisplatin toxicity.

The invention provides Chinese herbal medicine hair-nourishing and color-protecting cream, and belongs to the technical field of hair-protecting products. The invention relates to a Chinese herbal medicine hair nourishing and color protecting cream which can effectively solve various hair problems, such as alopecia, white hair, scurf, itching in head, folliculitis, dry and dry hair and the like. The hair nourishing cream provided by the invention is safe, non-toxic and free of side effects. When in use, the traditional Chinese medicine composition is externally applied to hair follicles at the roots of hair, and through the principle of transdermal administration, the traditional Chinese medicine composition deeply permeates into the skin layers of the hair follicles, activates hair cells and enables effective components in the Chinese herbal medicines to fully permeate into the hair follicles, so that the traditional Chinese medicine composition has a remarkable repairing effect on various hair problems and helps more people to get rid of hair troubles.

The disclosure provides polynucleotides containing regions of the Myosin 15 (Myo15) promoter, as well as vectors containing the same, that can be used to promote expression of a transgene specifically in hair cells. The polynucleotides described herein may be operably linked to a transgene, such as a transgene encoding a therapeutic protein, so as to promote hair cell-specific expression of the transgene. The polynucleotides described herein may be operably linked to a therapeutic transgene and used for the treatment of subjects having or at risk of developing hearing loss or vestibular dysfunction.

Disclosed herein are methods for treating and / or preventing AG-induced hearing loss. The methods involve downmodulating GABARAP disclosed herein to thereby treat and / or prevent AG-induced hearing loss. Also disclosed herein are methods for treating and / or preventing AG-induced hearing loss in a patient in need thereof comprising downmodulating GABARAP, disclosed herein, to thereby treat and / or prevent AG-induced hearing loss in a patient in need thereof.