54 results about "Hair cell" patented technology

The invention discloses application of a carrier carrying Pou4f3 genetic information in preparation of a medicine for treating DFNA15 deafness, and belongs to the technical field of gene therapy, the DFNA15 deafness is that POU4F3 is mutated, and the POU4F3 is mutated into POU4F3c. 337Cgt; the medicine for treating the T-type mutation DFNA15 deafness carries a carrier with Pou4f3 genetic information, the AAV carrier is a combination of AAV-Anc80L65 and a Myo15 promoter, and the AAV carrier is delivered to an inner ear through round window membrane injection, targets outer hair cells and compensates for insufficient haplodose of POU4F3. The invention verifies that AAV delivers POU4F3 gene to compensate c.337Cgt for the first time; deafness caused by dominant genetic mutation of DFNA15 can be corrected due to insufficient haplodose caused by T (p.Q113 *) mutation, the optimized AAV vector design breaks through the problem of insufficient hair cell targeting of a traditional vector, the treatment efficiency is remarkably improved, meanwhile, a P60-P180 dynamic monitoring system is established, it is proved that the curative effect is lasting, no side effect exists, and a reliable basis is provided for clinical transformation.

The invention relates to a dietary therapy formula for improving microcirculation of inner ears and a preparation method. Components with homology of medicine and food are selected, the black rice, the blueberries and the purple cabbages are rich in anthocyanin and beneficial to removing free radicals in cochlea and inhibiting hair cell apoptosis, the walnut kernels and the Chinese wolfberry fruits can improve blood vessel elasticity and protect auditory neurons, and the radix salviae miltiorrhizae, the hawthorn fruits and the radix puerariae can increase blood flow of inner ears, promote nutrition delivery, reduce blood viscosity and reduce vasospasm, so that the health-care food is suitable for being eaten by people. The whole component is low in sugar content, suitable for diabetics and capable of expanding the crowd. Effective components are processed into non-medicinal products, food therapy is beneficial to improving microcirculation of inner ears, relieving ear symptoms such as tinnitus and the like and delaying hearing loss, and a user can eat the food for a long time without psychological burden of medication.

The invention relates to the technical field of biological medicines, in particular to an adeno-associated virus AAV-IETP2 with a function of crossing a blood lost barrier and application of the adeno-associated virus AAV-IETP2. The adeno-associated virus AAV-IETP2 is prepared by inserting a nucleotide IETP2 oligopeptide capable of coding a blood lost barrier penetrating factor into an N end of a protein coding sequence of the adeno-associated virus VP2, and the nucleotide IETP2 oligopeptide has at least 80% of homology with a nucleotide sequence as shown in SEQ ID NO.1. The AAV-IETP2 disclosed by the invention can be administered through intravenous administration, and can penetrate through a blood labyrinth barrier to reach inner ear hair cells.

This utility model discloses a low-noise centrifugal pump for petrochemical applications, comprising a centrifugal pump body, an impeller movably mounted inside the pump body with an impeller shaft mounted on it, an inlet pipe at the front of the pump body, a drain pipe at the top of the pump body, and a low-noise mechanism wrapped around the outside of the pump body. The low-noise mechanism consists of noise reduction covers A, B, C, and D. Noise reduction cover B is fixedly mounted at one end of noise reduction cover A, and noise reduction cover D is fixedly mounted at one end of noise reduction cover C. This low-noise centrifugal pump for petrochemical applications features modularly designed noise reduction covers that can be quickly installed using a mounting bracket without complex tools or specialized skills, reducing the noise level generated by the centrifugal pump and preventing damage to the inner ear hair cells of factory workers, thus avoiding irreversible hearing loss. The rock wool itself has a porous fiber structure, primarily absorbing mid-to-high frequency noise.

The invention relates to the technical field of noise deafness susceptibility grading, in particular to a noise deafness susceptibility grading method and system based on a multi-band hearing threshold, and the method comprises the steps: presetting measurement frequency points, generating a measurement frequency point set according to a sequence from high frequency to low frequency, and completing M times of independent measurement for each frequency point; recording a tested subjective response timestamp, and generating a confidence value label; an otoacoustic emission signal in a tested ear canal is collected in real time, a time domain signal with the highest confidence coefficient of a single frequency point is extracted, spectrum features are calculated, and otoacoustic emission joint parameters are constructed. According to the method, the time sequence diffusion rule from high frequency to low and medium frequency hair cell damage can be comprehensively captured, the identification accuracy of potential auditory impairment is remarkably improved, the propagation mechanism from high frequency damage to intermediate frequency can be simulated by constructing the physically constrained spectrum migration operator, and otoacoustic emission time domain-frequency domain joint parameters and physiological indexes are combined, so that the identification accuracy of the potential auditory impairment is improved. And individualized and dynamic injury risk prediction is realized.

The invention belongs to the technical field of transgenic and biomedical research tools, and particularly relates to a method for constructing a transgenic fish system based on a Tol2 transposon system, which comprises the following steps: constructing a myo6b-lhfpl5b-DsRed plasmid by using myo6b as a promoter, lhfpl5b as a target gene and DsRed as a red fluorescent protein tag through a microinjection method, and constructing a Tol2 transposon system-based transgenic fish system. Co-injecting the plasmid containing the Tol2 transposase mRNA and the Tol2 transposase mRNA into a fertilized egg of an AB zebra fish strain, and screening generation by generation to obtain a transgenic fish strain with stable inheritance MET channel gene lhfpl5b marked hair cells. The spatio-temporal dynamic visualization of the living whole fish to the lhfpl5b is realized, the deafness-causing molecular pathway is clarified, and an in-vivo platform is provided for drug screening and intervention strategies.

The invention discloses an HSP70-targeting chimeric antigen receptor-macrophage and application thereof in treatment of noise-induced hearing loss, an extracellular domain of the HSP70-targeting chimeric antigen receptor comprises an HSP70 specific binding fragment, an intracellular domain comprises an IL-4 signal channel functional element, a signal switch type CAR structure of the HSP70 is formed, and the macrophage can be used for treating noise-induced hearing loss, so that the HSP70-targeting chimeric antigen receptor-macrophage can be used for treating noise-induced hearing loss. The HSP70-targeted chimeric antigen receptor-macrophage can be used for preparing the HSP70-targeted chimeric antigen receptor-macrophage, is used for mediating polarization of the macrophage to an M2 type and exerting an anti-inflammatory function, is proved to be capable of specifically responding to an inner ear inflammation microenvironment, switching an anti-inflammatory phenotype and protecting hair cells and auditory nerves, can be used for treating noise-induced hearing loss, and can be used for preparing the HSP70-targeted chimeric antigen receptor-macrophage. The limitation that traditional hearing-aid equipment supports treatment on symptoms is broken through, and the hearing-aid device has a remarkable application prospect.

The embodiment of the invention provides a cell guiding device, and the device comprises a receiving coil which is used for receiving a radio frequency signal transmitted by an in-vitro energy supply device; the stimulator is electrically connected with the receiving coil and is used for generating an electrical stimulation pulse according to the radio frequency signal; the electrode array is used for being arranged in the cochlea and electrically connected with the stimulator; the electrode array is used for applying the electrical stimulation pulse to hair cells in the cochlea so as to stimulate the hair cells to proliferate and differentiate. According to the technical scheme provided by the embodiment of the invention, the electrical stimulation pulse is generated according to the in-vitro radio frequency signal and is applied to the hair cells in the cochlea, so that accurate and efficient electrical stimulation on the hair cells can be realized. Meanwhile, the implanted design is adopted to directly act on the hair cells in the cochlea, so that the trauma to the body can be reduced, and the safety and comfort of treatment are improved.

The disclosure provides polynucleotides containing regions of the Myosin 15 (Myo15) promoter, as well as vectors containing the same, that can be used to promote expression of a transgene specifically in hair cells. The polynucleotides described herein may be operably linked to a transgene, such as a transgene encoding a therapeutic protein, so as to promote hair cell-specific expression of the transgene. The polynucleotides described herein may be operably linked to a therapeutic transgene and used for the treatment of subjects having or at risk of developing hearing loss or vestibular dysfunction.

The invention discloses a Pcdh15 gene enhancer sequence and application thereof, and belongs to the field of gene engineering, and the nucleotide sequence of the enhancer is shown as SEQ ID No.1. A mouse embryo microinjection system is used for verifying that the sequence is an enhancer, and the transcriptional activity of EGFP protein genes in hair cells can be remarkably enhanced in inner ears. Compared with other sequences with similar sizes, the enhancer sequence fragment can drive the expression ability of a reporter gene or a Pcdh15 gene to be obviously enhanced in inner ear hair cells, is specifically expressed in inner and outer hair cells, is suitable for biological materials such as recombinant DNA (Deoxyribonucleic Acid), vectors or adenoviruses and the like, and has a wide application prospect. The method is used for promoting transcription of EGFP genes or other genes in internal and external hair cells of inner ears.

The present disclosure describes gene therapy systems, and related methods, useful for treating and / or preventing deafness caused by genetic mutation of the TMPRSS3 gene or the LOXHD1 gene. The compositions and methods disclosed herein use adeno-associated viral (AAV) vector gene delivery of TRMPSS3 or LOXHD1 into the inner ear to restore activity of the TMPRSS3 gene or the LOXHD1 gene, respectively, promote hair cell survival and restore hearing in patients suffering from hearing loss. As disclosed herein, the systems and methods may utilize a combination of gene therapy (e.g., molecular therapeutics) for hearing loss caused by a genetic mutation together with implantation of a cochlear implant.

The invention relates to an application of glycyrrhizic acid-curcumin micelles in preparation of a medicine for treating deafness caused by gentamicin, and in the application, the glycyrrhizic acid-curcumin micelles are prepared from the following raw material medicines in percentage by weight: 66-89% of ammonium glycyrrhizinate and 11-34% of curcumin. According to the application, the glycyrrhizic acid-curcumin micelle can obviously improve hearing function impairment caused by drugs and obviously increase the fluorescent staining area of hair cells, and the effect of treating deafness caused by gentamicin is obvious.

The application discloses a kind of based on bionic lateral line's curved optical fiber multi-parameter sensor, one end of input optical fiber is connected with light source emission end, the other end is sequentially penetrated through the sensor base and artificial cilium cantilever beam and bare optical fiber fixed connection;The other end of bare optical fiber is fixedly connected with output optical fiber;The other end of output optical fiber is penetrated through artificial cilium cantilever beam and is connected with the light source receiving end communication connection.The sensor of the application can monitor the size of flow velocity, flow velocity direction, salinity and other multiple parameters simultaneously, and can meet the multidimensional monitoring demand under complex marine environment.Meanwhile, by imitating the structure of fish lateral line hair cell, using flexible artificial cilium cantilever beam as core perception element, the accurate detection of water flow velocity size and direction can be realized.In addition, the sensor is directly contacted with fluid through bare optical fiber part, and the detection of fluid salinity is realized by using the difference of different fluid refractive index.

The invention relates to application of ginkgo leaf exosomes in preparation of a medicine for treating hearing loss, and belongs to the field of medical technologies and biomedical materials. According to the application of the ginkgo leaf exosome in preparing the medicine for treating the hearing loss, the ginkgo leaf exosome can scavenge free radicals, so that inner ear hair cell apoptosis is inhibited, the inner ear active oxygen content is reduced, and the effect of treating the hearing loss is achieved. Therefore, the technical problems that in the prior art, the bioavailability of the ginkgo leaf extract is low, and the metabolism rate of active ingredients in the body is high are solved.

The disclosure provides polynucleotides containing regions of the Myosin 15 (Myo15) promoter, as well as vectors containing the same, that can be used to promote expression of a transgene specifically in hair cells. The polynucleotides described herein may be operably linked to a transgene, such as a transgene encoding a therapeutic protein, so as to promote hair cell-specific expression of the transgene. The polynucleotides described herein may be operably linked to a therapeutic transgene and used for the treatment of subjects having or at risk of developing hearing loss or vestibular dysfunction.

The invention belongs to the technical field of medicine, and particularly provides application of clotrimazole in preparation of a medicine for preventing and relieving cisplatin toxicity, the clotrimazole is an artificially synthesized imidazole antifungal compound, and the purity of the clotrimazole is 99.97% or above; the protective effect of clotrimazole in hair cells and hair cell lines of cochlea basilar membranes cultured in vitro is verified through HEI-OC1 cell culture and C57 mouse basilar membrane culture, and the in-vivo effect of clotrimazole is verified through tympanic injection of C57 mice; besides, the research test of clotrimazole for mouse cis-platinum ear toxicity shows that hearing thresholds of all frequencies after cis-platinum treatment are obviously increased, ABR threshold offset caused by cis-platinum is reduced by injection of clotrimazole into tympanic chambers, hair cell loss of all gynaecology is greatly improved due to the protection effect of clotrimazole, and the cis-platinum ear toxicity of mice is improved. Finally, the clotrimazole is proved to have a remarkable effect on prevention or treatment of cisplatin toxicity.

The invention provides Chinese herbal medicine hair-nourishing and color-protecting cream, and belongs to the technical field of hair-protecting products. The invention relates to a Chinese herbal medicine hair nourishing and color protecting cream which can effectively solve various hair problems, such as alopecia, white hair, scurf, itching in head, folliculitis, dry and dry hair and the like. The hair nourishing cream provided by the invention is safe, non-toxic and free of side effects. When in use, the traditional Chinese medicine composition is externally applied to hair follicles at the roots of hair, and through the principle of transdermal administration, the traditional Chinese medicine composition deeply permeates into the skin layers of the hair follicles, activates hair cells and enables effective components in the Chinese herbal medicines to fully permeate into the hair follicles, so that the traditional Chinese medicine composition has a remarkable repairing effect on various hair problems and helps more people to get rid of hair troubles.

The disclosure provides polynucleotides containing regions of the Myosin 15 (Myo15) promoter, as well as vectors containing the same, that can be used to promote expression of a transgene specifically in hair cells. The polynucleotides described herein may be operably linked to a transgene, such as a transgene encoding a therapeutic protein, so as to promote hair cell-specific expression of the transgene. The polynucleotides described herein may be operably linked to a therapeutic transgene and used for the treatment of subjects having or at risk of developing hearing loss or vestibular dysfunction.

Disclosed herein are methods for treating and / or preventing AG-induced hearing loss. The methods involve downmodulating GABARAP disclosed herein to thereby treat and / or prevent AG-induced hearing loss. Also disclosed herein are methods for treating and / or preventing AG-induced hearing loss in a patient in need thereof comprising downmodulating GABARAP, disclosed herein, to thereby treat and / or prevent AG-induced hearing loss in a patient in need thereof.

The invention relates to the technical field of sustained-release preparation, in particular to a microcrystalline sustained-release preparation and a preparation method thereof.The sustained-release preparation comprises a core composed of edaravone rodlike microcrystals and a shell composed of 2-4 double layers, the shell comprises at least one polyethylene glycol-polylysine copolymer inner layer, and the polyethylene glycol-polylysine copolymer inner layer is a polyethylene glycol-polylysine copolymer outer layer. And at least one outer layer of hyaluronic acid modified with a CD44 targeting peptide. The CD44 targeting peptide modified by the shell layer can specifically recognize a CD44 receptor on the surface of an inner ear hair cell, so that the binding efficiency of a preparation, a round window membrane and a target cell is remarkably improved, the diffusion of a drug to non-target tissues is reduced, and the local drug concentration is improved.

Presented herein are techniques that make use of objective measurements obtained in response to acoustic stimulation signals. More specifically, at least one measure of outer hair cell function and at least one measure of auditory nerve function are obtained from a tonotopic region of an inner ear of a recipient of a hearing prosthesis. The at least one measure of auditory nerve function and the least one measure of outer hair cell function are then analyzed relative to one another.

The invention relates to a dietary therapy formula for repairing ear hearing nerve cells and a preparation method thereof. Components with homology of medicine and food are optimally selected, the salvia miltiorrhiza and the radix puerariae can increase blood flow of inner ears, promote nutrition delivery, inhibit vasospasm of the inner ears and reduce ischemic injury of hair cells, alpha-linolenic acid is provided for a human body by utilizing the flaxseeds and the walnuts and converted into DHA, the DHA directly participates in nerve myelin sheath reconstruction, auditory nerve signal conduction is enhanced, and the health care effect is achieved. Chinese yam and astragalus membranaceus can provide Chinese yam polysaccharide and astragaloside, the mitochondrial function is improved, and cell apoptosis is delayed. The whole component is low in sugar content, is suitable for diabetics, expands the application crowd and non-medicine products, can reduce the psychological burden of eaters taking medicines for a long time, and is beneficial to popularization and application.

A method for treating hearing loss in a subject in need thereof is provided. The method includes providing a combination of transcription factors to induce generation of a hair cell-like cell, thereby treating the hearing loss in the subject. The generated hair cell-like cells exhibit characteristic of mature functional cells that is useful in cell replacement therapy for autologous transplantation.

Disclosed are compositions and methods for treating diseases of the mammalian eye, and in particular, complications of the retina associated with Usher syndrome 1B (USH1B). Further disclosed are compositions and methods for treating diseases of the mammalian inner ear, and in particular, complications of ear hair cells associated with Usher syndrome 1B (USH1B). The disclosure provides improved AAV-based, dual vector systems that facilitate the expression of full-length proteins whose coding sequences exceed that of the polynucleotide packaging capacity of an individual AAV vector. Described herein are modified hybrid dual vector systems that shift the coding sequence for the MYO7A tail domain from the front-half vector to the back-half vector by altering the split point (e.g., from between exons 23 and 24, to between exons 21 and 22), in order to eliminate the production of truncated MYO7A protein. Further described herein are improved, codon-modified hybrid and overlap vector systems in which putative stop codons and residual sequences in non-coding sequences are removed.

The invention discloses a cell culture device for hair regeneration, which comprises a culture medium in which a culture dish, a cell culture tank and a polystyrene microporous culture plate are arranged, and the culture medium is used for loading hair cells and a culture solution. The device is provided with an end cover, a temperature sensor and a PH sensor are installed on the end cover, and culture environment parameters can be monitored in real time. A PH value buffering assembly is arranged, a buffering solution in a bicarbonate buffering solution tank is conveyed to the culture dish through a water pump, and the PH value is adjusted. The device further comprises a temperature control assembly, a semiconductor chilling plate and a graphene electric heating piece are utilized, a heat conduction plate, a heat conduction copper pipe, heat dissipation fins and a heat dissipation fan are combined, and the culture temperature is adjusted. In the culture process, the sensor continuously monitors, all the components cooperatively work under the control of an external control system, the temperature and the PH value in the culture dish are kept within the range suitable for hair cell growth and regeneration, and effective cell culture support is provided for hair regeneration research.

The invention belongs to the technical field of biology, and particularly relates to an acoustic escape reaction detection device and a method for detecting zebra fish hair cell functions. The acoustic escape reaction detection device provided by the invention comprises the optical detection module, the illumination module, the culture module, the sound stimulation module and the transmission module, and special parameter design is carried out for the illumination module, so that a new method for detecting a to-be-detected sample by using the acoustic escape reaction is provided; the acoustic escape reaction detection device provided by the invention can more sensitively reflect the condition of functional damage of the sample, and is more convenient and faster to operate.

This invention discloses a hair cell protectant based on a ferritin nanoplatform and its application in treating hearing loss, belonging to the field of biomedicine. The ferritin nanoparticles of this invention, loaded with cimicifugin, include ferritin (Fn) as a carrier and cimicifugin (Ci@Fn) loaded within the ferritin. By loading cimicifugin (Ci), an extract of cimicifugin, into ferritin, it is endowed with additional anti-inflammatory and tissue-protective functions, thereby achieving a more precise and effective therapeutic effect. After injection through the round window membrane (RWM) behind the ear, Ci@Fn is transported to the basement membrane along with the flow of perilymph and endolymph, ultimately targeting the inner ear hair cells (HCs). After entering the hair cells, Ci@Fn is taken up and degraded by lysosomes, releasing cimicifugin (Ci), thereby exerting a protective effect on the hair cells.

The application discloses a chimeric antigen receptor-macrophage targeting HSP70 and application thereof in the treatment of noise-induced hearing loss, wherein the extracellular domain of the chimeric antigen receptor targeting HSP70 comprises an HSP70 specific binding fragment, and the intracellular domain comprises an IL-4 signal pathway functional element, forming a'signal switch type' CAR structure targeting HSP70, which can be used for preparing the chimeric antigen receptor-macrophage targeting HSP70 and mediating the polarization of the macrophage to M2 type and exerting the anti-inflammatory function. It has been verified that the chimeric antigen receptor-macrophage targeting HSP70 can specifically respond to the inner ear inflammatory microenvironment, switch the anti-inflammatory phenotype and protect the hair cells and auditory nerves, and can be used for treating the noise-induced hearing loss, breaking through the limitation of the symptomatic support treatment of the traditional hearing aid device and having a significant application prospect.

Presented herein are techniques that make use of objective measurements obtained in response to acoustic stimulation signals. More specifically, at least one measure of outer hair cell function and at least one measure of auditory nerve function are obtained from a tonotopic region of an inner ear of a recipient of a hearing prosthesis. The at least one measure of auditory nerve function and the least one measure of outer hair cell function are then analyzed relative to one another.

The invention belongs to the technical field of biological medicines, and particularly relates to application of neratinib in preparation of a medicine for preventing and treating noise-induced deafness. According to the specific technical scheme, the application of the neratinib in preparation of the medicine for preventing and treating hearing impairment / deafness is provided. According to the invention, the fact that neratinib can play a hearing protection role by specifically regulating and controlling cochlea immune microenvironment is found for the first time, and the hearing protection role is specifically realized by the following mechanisms: regulating and controlling biological effects such as cell signal channels, inflammatory response and cell stress response, protecting hair cell structures, and reducing cochlea basilar membrane outer hair cell loss caused by noise exposure. Based on the new discovery of the invention, the neratinib can be used for preparing medicines for preventing and treating hearing impairment and deafness caused by noise.