59 results about "Thermus thermophilus" patented technology

A DNA amplification method of amplifying DNA by PCR comprises a process of preparing a mixed solution for PCR reaction by mixing a template DNA, a primer DNA, a RecA protein derived from Thermus thermophilus (T. th. RecA protein) and a phage T4 gene 32 protein, and a process of amplifying DNA by subjecting the prepared mixed solution for PCR reaction to PCR reaction.

The invention relates to thermus thermophilus UTM802 preserved with CGMCC (China General Microbiological Culture Collection Center) No.6186. In a bacterial strain of the thermus thermophilus, organic waste is utilized as a carrier, solid-state high-temperature microbe compost bactericide is prepared in low cost and has no effect on moisture of materials during compost. In high-temperature compostby the solid-state high-temperature microbe compost bactericide, inactivation of active microbes in inoculant during high-temperature fermenting can be avoided, and the thermus thermophilus UTM802 has the advantages of stable performance and convenience in use.

The invention discloses a skin-care substrate, a preparation method and the application thereof, a cosmetic and a preparation method thereof, and relates to the technical field of cosmetics. The skin-care substrate comprises the following components: tetrahydromethyl pyrimidinyl carboxylic acid, a thermus thermophilus fermentation product, a buddleja officinalis extract, recombinant human-sourced collagen and water, wherein the weight percents of the tetrahydromethyl pyrimidinyl carboxylic acid, the thermus thermophilus fermentation product, the buddleja officinalis extract and the recombinant human-sourced collagen in the skin-care substrate are not lower than 1%. The problems that most of the conventional anti-aging products are based single antioxidation or single collagen supplement or the like, are not significant in anti-aging effect, and do not have an infrared-resisting effect are solved. By taking the tetrahydromethyl pyrimidinyl carboxylic acid, the thermus thermophilus fermentation product and the buddleja officinalis extract as main functional components in the skin-care substrate, and through synergistic compounding of the recombinant human-sourced collagen, and through a synergistic effect of the components, the skin-care substrate has the infrared-resisting and anti-aging efficacies.

The invention relates to a heatproof trehalose synthase fusion enzyme mutant gene and an application thereof. On the basis of optimized trehalose synthase of streptomyces roseus, an amino acid sequence capable of improving heat stability is linked to a C-end to construct to obtain a new fusion enzyme mutant; optimal reaction temperature of the trehalose synthase fusion enzyme mutant is increased from 20-25 DEG C to 50-55 DEG C, heat stability is also improved, yield of trehalose from catalytic maltose is 79% and higher than that, of 67%, of trehalose from Thermus thermophilus trehalose synthase, and the rehalose synthase fusion enzyme mutant is more beneficial for reducing production cost of the trehalose.

The invention discloses application of a fermentation product in preparing reagents for eliminating inflammation, moistening skin or whitening skin, the fermentation product is prepared from thermus thermophilus and yeast through fermentation co-culture, and a thermus thermophilus and yeast co-fermentation method comprises the following steps: firstly, carrying out bacterium increase fermentationonce or for multiple times on yeast at a low temperature under an acid condition, keeping in an index growth period, increasing the temperature of a fermentation liquid to a high temperature from a low temperature, adding thermus thermophilus, and carrying out secondary fermentation under an alkali condition. By adopting the method, active components of the thermus thermophilus can be remarkably enriched, particularly the contents of active components used in skin caring preparations can be increased, or the activity of the active components can be improved, and particularly the fermentation product has remarkable effects of gentle inflammation elimination, skin moistening and skin whitening.

The invention provides an X-ray crystal structure of the 30S ribosome, obtained from Thermus thermophilus 30S subunit, having a tetragonal space group P4₁2₁2 with unit cell dimensions of a=401.4±4.0 Å, b=401.4±4.0 Å, c=175.9±5.0 Å. An advantageous feature of the structure is that it diffracts beyond 3 Å resolution. The invention also provides a crystal of 30S having the three dimensional atomic coordinates of the 30S ribosome, the coordinates being provided in Tables 1A and 1B. The data may be used for the rational design and modelling of inhibitors for the 30S ribosome, which have potential use as antibiotics.

The invention provides an X-ray crystal structure of the 30S ribosome, obtained from Thermus thermophilus 30S subunit, having a tetragonal space group P4₁2₁2 with unit cell dimensions of a=401.4±4.0 Å, b=401.4±4.0 Å, c=175.9±5.0 Å. An advantageous feature of the structure is that it diffracts beyond 3 Å resolution. The invention also provides a crystal of 30S having the three dimensional atomic coordinates of the 30S ribosome, the coordinates being provided in Tables 1A and 1B. The data may be used for the rational design and modelling of inhibitors for the 30S ribosome, which have potential use as antibiotics.

The invention relates to a centella asiatica fermentation liquor, a preparation method and application of the centella asiatica fermentation liquor, wherein the centella asiatica fermentation liquor can be used for converting high-content madecassoside and producing high-activity asiatic acid and derivatives of the high-activity asiatic acid by utilizing a microbial conversion reaction. After centella asiatica is fermented by thermus thermophilus, the centella asiatica is converted to generate the madecassoside and asiatic acid, the content of madecassoside is increased by 200-8000 times, and the effects of the fermented centella asiatica, such as inflammation resistance, wound repair, oxidation resistance, whitening and skin cell repair capability (light damage repair and scratch repair) are also remarkably improved.

The invention discloses a discontinuous xylo-pfan isomerase and its genes. The discontinuous xylo-pfan isomerase selects form Thermus thermophilus and its amino acid sequence is indicated by SEQ ID NO.2. The said discontinuous xylo-pfan isomerase has improved specificity to the substrate D-wood sugar without changing original heat endurance. The discontinuous xylo-pfan isomerase can be used in miscible liquids including wood sugar and amylaceum to specially detect the wood sugar content and can be used in constructing recombination bacteria with wood sugar.

The invention provides a nursing composition containing symbiotic bacterium combined fermented products, for resisting ageing and repairing skin, essence lotion, a preparation method of the nursing composition containing symbiotic bacterium combined fermented products, for resisting ageing and repairing skin, and an application of the essence lotion. The nursing composition containing symbiotic bacterium combined fermented products, for resisting ageing and repairing skin comprises the symbiotic bacterium combined fermented products, decarboxy carnosine HCl, palmitoyl pentapeptide-4, tocopheiyl acetate, a pale butterflybush flower extract, resveratrol, a pomegranate fruit extract and a narcissus bulb extract, wherein symbiotic bacteria of the symbiotic bacterium combined fermented productscomprise combination of schizophyllum commune, thermus thermophilus, microzymes and lactobacilli, and fermentation raw materials of the symbiotic bacterium combined fermented products comprise combination of rice, coix lacryma-jobi ma-yuen seeds, ginseng, kelp and raspberries. Through synergistic compounding of effective active components of the symbiotic bacterium combined fermented products andthe like, the nursing composition containing symbiotic bacterium combined fermented products, for resisting ageing and repairing skin can condition and repair skin ageing resistant routes of oxidation resistance, saccharification resistance, blue light resistance, collagen synthesis promotion and the like, so that cell vitality is protected, and the skin has the capacity for resisting exogenous environmental factors.

The invention discloses a thermus thermophilus laccase (benzenediol: oxygen oxidoreductases), engineering bacteria, a recombinant laccase and a use of the recombinant laccase. The thermus thermophilus laccase has an amino acid sequence shown in the formula of Seq No.2. The DNA sequence for coding the above laccase is shown in the formula of Seq No.1. The DNA can be used in construction of a Pichia pastoris expression system. Through expression vector pHKFA1-lacTT linearization and then Pichia pastoris conversion by the expression vector, recombinant Pichia pastoris engineering bacteria are obtained. The recombinant laccase is obtained by fermentation of the recombinant Pichia pastoris engineering bacteria. The recombinant laccase has laccase activity and high temperature resistance, can be used in dye decoloring and especially in azo dye and anthraquinone dye decoloring, and has a decolourization ratio of 100%.

The invention discloses a method for realizing over expression of the thermostable laccase gene through location transformation. Thermostable laccase produced from thermus thermophilus bacteria has great application potential in the industrial processes of pulp bleaching, textile decoloring, biomass hydrolysate detoxifying and the like, but the thermostable laccase gene has low expression level and can not become an enzyme source. In the invention, a carrier in a pHsh series is firstly applied to carrying out cloning, in-situ mutagenesis and induced expression on the laccase gene. By carryingout case analysis and multiple explorative site-directed mutagenesis on a special sequence of the thermostable laccase gene, the invention has the effects of reducing rare codons in the gene, lowering the GC base content and weakening formation of a nucleic acid secondary structure and the like, thereby enhancing the expression level of the gene in escherichia coli by 317 times compared with a natural gene.

The invention discloses a thermophilic neutral protease gene, engineering bacteria JD-TP (Escherichia coli) producing thermophilic neutral protease, thermophilic neutral protease produced by fermenting the engineering bacteria, mature thermophilic neutral protease after self-catalytic shearing and an application of the thermophilic neutral protease and the mature thermophilic neutral protease in degrading proteins, and belongs to the technical field of bioengineering. The thermophilic neutral protease gene is obtained by amplifying a thermophile Thermus thermophilus HB8 genome and is connected to an expression vector to be successfully expressed in escherichia coli; the optimum reaction temperature of the thermophilic neutral protease is 85 DEG C, the half life at 85 DEG C is 8d, and the optimum reaction temperature of the mature thermophilic neutral protease is 75 DEG C; the optimum pH of the thermophilic neutral protease is 7.5-10.0, and the optimum reaction pH of the mature thermophilic neutral protease is 6.5; the thermophilic neutral protease and the mature thermophilic neutral protease have very high optimum reaction temperatures, thermal stability and relatively wide reaction pH values and have a very good industrial application prospect.

The invention provides an amino acid surfactant composition containing a symbiotic bacterial composite fermentation product, a facial cleanser and a preparation method of the facial cleanser. The amino acid surfactant composition comprises the symbiotic bacterial composite fermentation product, compound amino acid, potassium cocoyl glycinate, sodium methyl cocoyl taurate, lauryl hydroxysultaine and disodium cocoamphodiacetate, wherein symbiotic bacteria of the symbiotic bacterial composite fermentation product comprise a combination of schizophyllum commune, thermus thermophilus, saccharomycetes and lactobacillus; and fermenting raw materials of the symbiotic bacterial composite fermentation product comprise rice, coix lacryma-jobi ma-yuen seeds, ginseng, giant kelp and raspberries. The facial cleanser prepared from the amino acid surfactant composition is high in foaming speed, has smooth and dense foam, relatively strong cleaning performance and relatively good moistening effect, ismild and non-irritant, cannot injure the skin barrier after using, and enables the skin to be refreshing but not tight.

The invention discloses a biological agent for improving aerobic fermentation efficiency of organic waste. The biological agent is prepared from the following raw materials: 40-60 parts of bacillus stearothermophilus, 40-60 parts of thermophilic bacteria, 40-60 parts of lactobacillus thermophilus, 20-30 parts of bacillus subtilis, 20-30 parts of bacillus subtilis subsp. subtilis, 10-20 parts of alcaligenes, 10-20 parts of bacillus circulans, 10-20 parts of aspergillus fumigatus, 5-15 parts of trichoderma viride, 5-15 parts of geotrichum candidum, 3-10 parts of white rot fungi, 3-10 parts of brown rot fungi, 1-5 parts of green algae and 1-5 parts of blue algae. A preparation method comprises the following steps of: (1) weighing the raw materials; (2) activating strains; (3) compounding mixed strains; (4) preparing a primary seed solution; and (5) preparing the biological agent. The biological agent disclosed by the invention can reduce generation and emission of odor in a composting process and reduce loss of nutrient substances such as nitrogen, so that the aerobic fermentation efficiency of the organic waste is improved.

The invention relates to the technical field of microorganisms, and discloses application of GSH synthesis and circulation related genes as well as a recombinant saccharomyces cerevisiae strain. The recombinant saccharomyces cerevisiae strain with excellent stress resistance is constructed by selecting GSH synthesis and circulation related genes from kluyveromyces marxianus, marine acid-resistant bacteria walleria mellica and extreme thermus thermophilus HB27. Compared with wild saccharomyces cerevisiae, the recombinant saccharomyces cerevisiae shows different effects in H2O2 resistance, heat shock resistance, high temperature resistance, acid resistance and salt resistance according to different exogenous genes; and an excellent yeast strain is provided for industrial fermentation production, and also a cell model is provided for later research on generation or protection of ROS in eukaryotes.

An object of the present invention is to provide a nucleic acid amplification method for amplifying a desired nucleic acid while suppressing amplification of byproducts in a PCR reaction, a reagent kit used for nucleic acid amplification, a method of detecting single nucleotide polymorphism to detect single nucleotide polymorphism by utilizing that amplification of byproducts is suppressed in a PCR reaction, and a reagent kit used for detecting single nucleotide polymorphism. The method of amplifying nucleic acids by PCR is characterized by admixing in a reaction solution, a homologous recombinant protein which contains at least one of a RecA protein derived from Thermus thermophilus, and a modified RecA protein obtained by modification of the RecA protein and having a function similar to that of the RecA protein, and carrying out PCR.

The invention clones a heat resistant alkaline phosphatase gene from a high-temperature bacteria Thermus thermophilus XM genome. Active polypeptide (enzyme) or modified enzyme modified in genetic engineer which is encoded by the gene can be widely used in research field of diagnostics, amynology, biological chemistry and molecular biology. The invention firstly relates to extraction of Thermus thermophilus XM genome DNA, secondly PCR amplification and sequence analysis of heat resistant alkaline phosphatase gene (tap gene), thirdly expression and purification of heat resistant alkaline phosphatase, fourthly measurement to enzymology property of recombination of alkaline phosphatase.

A synthesis method of aromatic amino acids according to one aspect of the present invention includes a process of preparing thermostable enzyme, a process of amino-transferring reaction and a process of product precipitation and enzyme recycling. The process of preparing thermostable enzyme comprises an isolation step of the gene of Thermus thermophilus aspartate aminotransferase (TtAspAT), a construction step of the TtAspAT gene in an expression vector, and a transformation step of host cells with the TtAspAT gene-harboring vector for producing a plurality of TtAspAT. The process of amino-transferring reaction catalyzed by TtAspAT, in which an amino group from an amino donor is transferred to the carbonyl group of a keto acid (the amino acceptor) through an amino-transferring reaction at the temperature between 50° C. and 80° C. for a reaction time from 30 to 90 minutes to produce aromatic amino acids. The process of product precipitation and enzyme recycling is used to recover and purify the synthesized amino acids and recycle the active aminotransferase in the supernatant of a reaction mixture.