Heatproof trehalose synthase fusion enzyme mutant gene and application thereof

Abstract

The invention relates to a heatproof trehalose synthase fusion enzyme mutant gene and an application thereof. On the basis of optimized trehalose synthase of streptomyces roseus, an amino acid sequence capable of improving heat stability is linked to a C-end to construct to obtain a new fusion enzyme mutant; optimal reaction temperature of the trehalose synthase fusion enzyme mutant is increased from 20-25 DEG C to 50-55 DEG C, heat stability is also improved, yield of trehalose from catalytic maltose is 79% and higher than that, of 67%, of trehalose from Thermus thermophilus trehalose synthase, and the rehalose synthase fusion enzyme mutant is more beneficial for reducing production cost of the trehalose.

Description

technical field [0001] The invention relates to the fields of genetic engineering and enzyme engineering, in particular to a heat-resistant trehalose synthase fusion enzyme mutant gene and its application. The trehalose synthase expressed by the gene can be used to produce trehalose. Background technique [0002] Trehalose is a non-reducing disaccharide composed of two molecules of glucose connected by α,α-1,1 glycosidic bonds. It widely exists in microorganisms, shrimps, Saccharomyces cerevisiae, mushrooms and various fungi, insects, plants, etc. Exogenous trehalose also has a good non-specific protective effect on organisms, biological macromolecules, and biofilms. It can protect cells from damage caused by environmental changes (such as dehydration, hypertonicity), and the specific performance is Effective protection of biofilm, protein and DNA, etc., so it is known as "sugar of life". The content of trehalose in nature is very low. In the past, it was mainly extracted f...

AI Technical Summary

Problems solved by technology

Although the heat-resistant trehalose synthase has the characteristics of high optimum reaction temperature and good thermal stability, due to the higher reaction temperature, the generation of glucose as a by-product will be increased, thereby reducing the conversion rate of the substrate. At the same time, heat-resistant and heat-resistant trehalose synthase also has the characteristics of large molecular weight and slow reaction speed

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