3 results about "Laccase activity" patented technology

This invention discloses a bimetallic co-doped carbon-based nanozyme, its preparation method, and its applications, belonging to the fields of nanomaterials and biomimetic enzyme technology. Using tannic acid and melamine as carbon and nitrogen sources, and introducing iron and manganese salts, this invention successfully prepared an iron-manganese bimetallic co-doped carbon-based nanozyme through a simple mixing, evaporation, and high-temperature calcination method. The resulting nanozyme exhibits an ultrathin nanosheet structure, with Fe and Mn elements uniformly dispersed within the carbon framework. This nanozyme demonstrates significantly enhanced laccase-like activity, with a specific activity reaching 3.4 U / mg, 6.8 times that of natural laccase from Trametes versicolor, and exhibits excellent pH and temperature stability as well as high substrate affinity. The preparation method of this invention is simple, low-cost, and easily scalable. This bimetallic co-doped carbon-based nanozyme has broad application prospects in the fields of catalytic degradation and colorimetric detection and analysis of organic pollutants.

The application belongs to the technical field of analysis and detection, and particularly relates to a high-activity nano-enzyme for simultaneously detecting various chlorophenols, a preparation method and application thereof. The nano-enzyme is a composite material formed by in-situ chelation of copper nitrate, adenine and hierarchical porous cerium metal organic framework HM UIO-66(Ce). The copper-adenine formed by coordination of copper nitrate and adenine is uniformly loaded on the surface or pore interface of the hierarchical porous cerium metal organic framework HM UIO-66(Ce) and forms a stable combined structure. The high-activity nano-enzyme has excellent laccase activity. Based on the difference in catalytic reaction kinetics of different chlorophenols, a colorimetric sensing array for detecting various chlorophenols is constructed, so that the recognition and analysis of various target substances are realized. The method does not need to rely on a complex electrode system or various buffer conditions, and has the characteristics of stable structure, simple operation and suitability for rapid detection.

This invention discloses a high-performance laccase mutant constructed based on multi-source gene recombination and its applications. Using BspLac from Bacillus sp. SL-1 and BLTCLac from Bacillus licheniformis TCCC111219 as templates, a mutant library DS was constructed using DNA shuffling technology. Two high-performance laccase mutants, DS2 and DS5, were obtained through screening. Dynamic enzyme production analysis showed that the laccase activity of DS2 peaked at 1922 U / L after 24 hours, equivalent to twice that of the original strain; the laccase activity of DS5 peaked at 1337 U / L after 24 hours, equivalent to 1.4 times that of the original strain. Simultaneously, experimental verification showed that DS2 and DS5 achieved degradation rates of 83% and 89% for malachite green, respectively. This laccase mutant significantly improved the detoxification efficiency of synthetic dyes and possesses good potential for industrial application.