The invention discloses a culture medium for fermenting panus rudis to produce laccase and a laccase production method. A culture medium formula comprises 10.04g/L of soluble starch, 0.2g/L of peptone, 0.50g/L of K2HPO4, 0.50g/L of KH2PO4, 0.01g/L of ZnSO4.7H2O, 0.5g/L of MgSO4.7H2O, 0.01g/L of CuSO4.7H2O, 0.005g/L of FeSO4.7H2O, 0.05g/L of MnSO4, 0.08g/L of CaCl2, 0.1g/L of VB1, 0.4% of tween-40, and 0.05g/L of veratryl alcohol; the initial pH value of the culture medium is 6.8. A fermentation seed solution is inoculated in the culture medium so as to culture and produce the laccase at the temperature of 31 DEG C and the rotating speed of 160r/min. The culture medium for fermenting the panus rudis to produce the laccase, provided by the invention, has the advantages of high laccase productivity, high laccase activity and the like; the culture method is short in period and mild in conditions.