43 results about "Laccase activity" patented technology

The invention belongs to the field of microorganisms, and particularly relates to bacillus Z161 capable of efficiently degrading straw as well as a screening method and application of the bacillus Z161. The bacillus subtilis Z161 is bacillus mexicanus, the preservation number of the bacillus mexicanus Z161 is CGMCC (China General Microbiological Culture Collection Center) NO.36393, and the bacillus mexicanus Z161 is preserved in the China General Microbiological Culture Collection Center on October 29, 2025. The bacillus Z161 screened by the invention has strong laccase activity and high temperature resistance, and can efficiently degrade lignin under the condition of 50 DEG C; the problem that in the prior art, normal-temperature zymophyte is mostly adopted, and the requirement for fermentation conditions in the fermentation process is harsh is solved, and therefore the rapid compost fermentation technology is easier to achieve. The bacillus Z161 provided by the invention has the advantages of genetic and functional character stability, has good tolerance at high temperature, enables lignin to be quickly depolymerized, and improves the decomposition efficiency.

The invention discloses a method for detecting pesticide based on a colorimetric sensing array of a copper-based MOF composite material, and relates to the technical field of pesticide detection.The method comprises the steps that a Co, Zn and Cu-MOF (VB2) composite nano-enzyme material with peroxidase-like activity and laccase-like activity is prepared; before and after a pesticide analyte to be detected is added, the peroxidase-like activity and laccase-like activity of the Co, Zn and Cu-MOF (VB2) composite nano-enzyme material catalyze different substrates in a neutral environment, so that catalytic products have different colorimetric responses to detect the pesticide analyte to be detected. The method can be used for simultaneously distinguishing and detecting five pesticides including carbendazim, pendimethalin, thiophanate-methyl, triadimefon and acetamiprid, and has the advantages of simplicity and convenience in operation and high detection speed.

The invention discloses a method for efficiently expressing laccase by regulating endoplasmic reticulum stress reaction of coprinus cinereus, and belongs to the field of gene engineering and fermentation engineering. According to the invention, by regulating endoplasmic reticulum stress reaction action genes der1, Anhac1 and Cchac1 of filamentous fungi coprinus cinereus, recombinant extracellular expression of laccase Lcc5 is finally improved and regulated. Wherein the extracellular laccase activities of the recombinant bacteria can be respectively improved by 18%, 70% and 26% through interference of der1, overexpression of Anhac1 and overexpression of Cchac1, and the obtained extracellular laccase activities are respectively 31.4 U / mL, 45.1 U / mL and 33.6 U / mL, which is the highest level of coprinus cinereus homologous recombination expression laccase Lcc5 reported at present. The invention firstly indicates that the regulation and control of the coprinus cinereus endoplasmic reticulum stress reaction can promote the efficient expression of laccase in coprinus cinereus, and the invention further promotes the development of a coprinus cinereus expression system.

The invention discloses an Hg < 2 + > detection method for enhancing Hg < 2 + > intrinsic laccase-like activity based on ZIF-8-Au. The invention finds that the mercury ions have laccase-like activity, the enzyme activity of the mercury ions is obviously enhanced under the action of ZIF-8-Au, and the color of the reaction catalyzed by the mercury ions is obviously changed along with the increase of the concentration of Hg < 2 + >, so that a colorimetric analysis method for detecting the mercury ions is developed on the basis, the Turn-On type visual analysis is realized, and the application prospect is wide. The method realizes simple, rapid, sensitive and high-selectivity detection of Hg < 2 + > residues in water and food. The invention provides a new method and principle for the fields of Hg < 2 + > environment monitoring and food safety, and provides a new direction for the development of a heavy metal ion detection technology.

The invention provides a laccase gene LAC18 screened from apples and aphid resistance application of the laccase gene LAC18 in catalytic synthesis of plant lignin, the nucleotide sequence of the gene is SEQ ID NO: 1, and the amino acid sequence of encoded protein of the gene is SEQ ID NO: 2. According to the method, the aphid resistance of the transgenic tobacco is comprehensively evaluated by inoculating aphids on leaves of the transgenic laccase gene tobacco and observing indexes such as the survival rate and the aphid yield. The result shows that the laccase activity and the lignin content of the transgenic laccase tobacco are remarkably improved, the lignin of cell walls is thickened, and the lignification degree is remarkably improved. The translaccase gene tobacco not only can effectively reduce the survival rate of myzus persicae, but also has a remarkable inhibition effect on the average reproductive capacity of the myzus persicae, and the aphid resistance of plants is greatly improved.

The application discloses a silver-based metal organic framework nano-enzyme loaded with silver nanoparticles and having multi-enzyme-like activity, and a preparation and application thereof. The application uses 2-methyl imidazole as an organic ligand, triethylamine as a deprotonation reagent and a basic environment provider, methanol as a solvent and a silver ion reducing agent, and obtains a silver-based metal organic framework by using the coordination of the deprotonated 2-methyl imidazole and the silver ion. Meanwhile, the silver ion adsorbed on the surface of the silver-based metal organic framework is reduced into silver nanoparticles by using methanol under the alkaline condition, and a silver-based metal organic framework nano-enzyme loaded with silver nanoparticles is prepared. The nano-enzyme can exhibit multi-enzyme-like activities such as oxide enzyme-like activity, peroxide enzyme-like activity and laccase enzyme-like activity under different conditions, and can realize the colorimetric detection of a plurality of trace analysis objects in different types of complex samples by using different enzyme-like activities under different detection conditions.

The invention provides an efficient microbial fermentation inoculant for straws and a preparation method thereof, and relates to the technical field of biodegradation. The efficient microbial fermentation inoculant for the straw is formed by compounding four strains including trichoderma reesei, streptomycete, lactobacillus plantarum and bacillus amyloliquefaciens according to the mass ratio, the ratio of the viable count of the four strains is that the ratio of the viable count of the trichoderma reesei to the viable count of the streptomycete to the viable count of the lactobacillus plantarum to the viable count of the bacillus amyloliquefaciens is 3: 2: 2: 1.5, the total viable count of the inoculant is larger than or equal to 1.3 * 10 CFU / g, and the cellulase activity is larger than or equal to 85 U / g. The laccase activity is greater than or equal to 42U / g. The invention can effectively solve the industrial pain points of difficult degradation of straws, poor quality and weak stability, and in agricultural production, the microbial inoculum can increase the content of organic matters in soil, can reduce the feed cost in the feed field, and can be used for production of straw-based organic fertilizers in the environmental protection field, and can reduce the use amount of chemical fertilizers.

The invention discloses a colorimetric detection method for daucoside based on enhanced octahedral silver oxide laccase activity and application, and belongs to the technical field of agricultural product safety detection. According to the present invention, the nucleic acid aptamer capable of being specifically combined with the danioside is adopted as the original recognition element, and the colorimetric detection method for enhancing the activity of the octahedral silver oxide laccase by using the nucleic acid aptamer is constructed, and is used for rapid danioside detection; the method is high in sensitivity and strong in specificity, the lowest detection limit of the droomcorn glycoside is 0.0743 mu g / mL, the color change can be distinguished by naked eyes, the method has the advantages that the operation is simple and convenient, the reaction is rapid, large-scale instruments are not needed and the like, the recovery rate in a sorghum sample reaches 89.66%-100.87%, and the method is suitable for on-site screening of the droomcorn glycoside in practical samples such as sorghum.

The present application relates to a kind of preparation methods of phaeolus schweinitzii fungicide, phaeolus schweinitzii (P. Phlebiopsis pilatii ) HE39 is inoculated medium fermentation culture, centrifugation, and the supernatant is taken;The preservation number of phaeolus schweinitzii (P. Phlebiopsis pilatii ) HE39 is CGMCC No.41168.The phaeolus schweinitzii fungicide prepared in the present application can be used for degrading lignin and cellulose, has good degradation ability to lignin in ground combustible, has good laccase activity, lignin peroxidase and manganese peroxidase activity, and can improve the weight loss rate of forest combustible.

The present application provides a kind of marine bacteria capable of degrading lignin and removing nitrite, which is a deep-sea seabed sulfide source, and has the function of degrading lignin and removing nitrite.The strain of Halomonas sp.has a preservation number of CGMCC No.34690.The strain screened in the present application can grow and reproduce in seawater, can utilize guaiacol as a carbon source, and can remove nitrite.The strain of the present application has laccase activity, has laccase gene and nitrite reductase gene.The experimental results show that the cumulative removal rate of guaiacol after 2 days of culture is 56%, and the removal rate of nitrite after 4 days of culture is close to 70%, which has great application potential in the marine aquaculture industry.

This invention discloses a laccase derived from *Coprinus gracilis*, its recombinant expression strain, and a highly efficient expression method, belonging to the fields of microbiology and bioengineering technology. Using *Aspergillus niger* MA70.15 as the experimental strain, this invention employs a random integration strategy to achieve pC3-P... citA -SP GlaA - lcc9‑ T trpC ‑pyrG The expression vector was integrated into the upstream region of the promoter of the host bacterium citrate synthase in the form of nine consecutive copies. To investigate the effects of microparticles and osmotic regulators in the fermentation medium on laccase production by recombinant Aspergillus niger, this invention added different types and concentrations of microparticles and osmotic regulators, resulting in a laccase activity of 1566.7 U / L in shake-flask fermentation of recombinant strain AnRcgL1 and 1961 U / L in a 1 L fermenter, successfully achieving efficient recombinant expression of laccase Lcc9 in Aspergillus niger.

The invention belongs to the technical field of microorganisms, and particularly relates to bacillus velezensis, a straw degradation agent, a preparation method, a straw degradation method and application. The invention provides a bacillus velezensis LLS, and the preservation number of the bacillus velezensis LLS is CGMCC (China General Microbiological Culture Collection Center) No.35634. The bacillus velezensis LLS provided by the invention is high in cellulase total activity, endo beta-1, 4-glucosidase activity, exo beta-1, 4-glucosidase activity, beta-1. 4-glucosidase activity, lignin peroxidase activity, laccase activity and manganese peroxidase activity, can tolerate low temperature, and can be used for preparing the bacillus velezensis LLS. The straw degradation effect is good under the low-temperature condition.

The invention discloses a laccase mutant S186D and application of the laccase mutant S186D in catalyzing oxidative polymerization of cardanol. The invention provides a laccase mutant, the laccase mutant is characterized in that the 186th amino acid serine of laccase cotABs is mutated into other amino acids, and the amino acid sequence of the laccase cotABs is as shown in SEQ ID NO: 1. According to the laccase mutant CotAS186D disclosed by the invention, amino acid at the 186th site of the laccase from bacillus subtilis B.subtilis 168 is mutated from serine S to aspartic acid D (laccase mutant CotAS186D) in a site-specific mutagenesis manner, the catalytic activity of the laccase is remarkably improved, and compared with wild type laccase, the CotAS186D shows remarkably improved laccase activity and is more suitable for industrial production. Experiments show that a recombinant strain based on the expression laccase mutant has excellent CotAS186D production capacity, and has a better industrial application prospect.

This invention discloses a method for detecting octopamine and tyramine using a laccase-like Schiff base Cu-MOF nanozyme. The method uses a Schiff base formed from glutaraldehyde and 2-aminoterephthalic acid as starting reagents as ligands to synthesize a flower-like Cu-MOF nanozyme. This Cu-MOF exhibits near-infrared light-triggered laccase-like activity. Using octopamine and tyramine as substrates, Cu-MOF catalyzes the oxidation of octopamine and tyramine, conjugating 4-aminoantipyrine to form a red coupling product. This product exhibits a high absorption spectrum at 505 nm, demonstrating high detection efficiency with detection limits of 0.18 μg / mL and 0.60 μg / mL, respectively. X-ray photoelectron spectroscopy analysis shows that the Cu(I) / Cu(II) redox reaction participates in the catalytic process. This method is characterized by high sensitivity, strong specificity, simple operation, and rapid response.

Provided is a new CueO mutant with improved activity compared with wild-type CueO. The protein according to one or more embodiments of the present invention contains an amino acid sequence [1] or [2] and has laccase activity: [1] an amino acid sequence containing at least a region of positions 29-516 in SEQ ID NO: 14 with mutations (a) and (b): (a) a substitution of D360 with an amino acid other than D; and (b) one or more selected from a substitution of G304 with an amino acid other than G, a substitution of D373 with an amino acid other than D, and a substitution of Q374 with an amino acid other than Q; or [2] an amino acid sequence 90% or more identical to the sequence [1], and having residues corresponding to positions 360, 304, 373, and 374 of SEQ ID NO: 14 identical to those of the sequence [1].

The application discloses a PtIrRuCuPd high-entropy alloy nanoscale enzyme and a preparation method and application thereof, and belongs to the field of catalytic materials and analytical chemistry. The high-entropy alloy nanoscale enzyme is a nanoparticle composed of five elements of Pt, Ir, Ru, Cu and Pd, and has a quasi-spherical structure. The noble metal elements Pt, Ir, Ru and Pd are combined with the transition group metal element Cu, a multi-element synergistic effect is caused, the peroxidase activity, the oxidase activity and the laccase activity are improved, and the high-entropy alloy nanoscale enzyme has optimal comprehensive enzyme activity. The high-entropy alloy nanoscale enzyme prepared by the application realizes the synchronous enhancement of POD-like, OXD-like and LAC-like activities, a multi-channel sensing array is constructed, different responses of bisphenol substances are generated through different catalytic paths, and high-selectivity recognition and trace detection of bisphenol A in a complex sample are realized by combining hierarchical clustering analysis and linear discriminant analysis algorithms. The application provides a reference for the design of a novel multi-activity nanoscale enzyme and the development of an advanced sensing array.

A method for dual-mode detection of beta-lactam antibiotic residues based on laccase-like activity nano-enzyme Cu-MOF is characterized in that a copper metal organic framework (Cu-MOF) with laccase-like activity and blue fluorescence is prepared by adopting a hydrothermal method, color change is generated in the process of oxidizing o-phenylenediamine (OPD) to generate a yellow product 2, 3-diaminophenazine (DAP), and the color change is changed in the process of oxidizing o-phenylenediamine (OPD) to generate the yellow product 2, 3-diaminophenazine (DAP). And dual-mode signal output of colorimetric and ratiometric fluorescence is realized. When the BLs exist, the BLs and OPD are competitively combined with Cu-MOF active sites, generation of DAP is inhibited, a colorimetric signal is weakened, blue fluorescence is recovered, and therefore high-sensitivity and high-selectivity detection of the BLs is achieved. According to the invention, a portable on-site quantitative detection platform is established by further combining smartphone image acquisition and RGB analysis technologies, is successfully applied to rapid and accurate detection of BLs residues in animal-derived foods such as milk and pork, and has the advantages of simplicity and convenience in operation, low cost, on-site applicability and the like.

The invention discloses a double-enzyme active nano-enzyme, a preparation method thereof and application of the double-enzyme active nano-enzyme in profenofos bimodal detection, the double-enzyme active nano-enzyme is UiO-66 (Ce)-Cu, has efficient and balanced phosphohydrolase and laccase activity, and has high sensitivity and selectivity in self-cascade detection of profenofos; according to the preparation method, a solvothermal method is utilized to add copper on UiO-66 (Ce) for modification, so that the double-enzyme active nano-enzyme can be effectively prepared; the invention also constructs a colorimetric method and a fluorescence method for detecting profenofos by using the double-enzyme active nano enzyme, and the colorimetric detection method is simple and convenient to operate, efficient in process, low in detection limit and visual and clear in result; a fluorescence detection method is high in speed and short in time consumption, and a detection result can be obtained in only four minutes; the two detection methods can sensitively, specifically and accurately detect the residual profenofos in the vegetables.

The invention belongs to the technical field of nano-enzyme preparation and sensing, and particularly relates to a laccase-like fluorescent nano-enzyme and application of the nano-enzyme in epinephrine dual-mode detection. The nano enzyme can realize the integration of the catalytic activity and the fluorescence property of imitated laccase in a single inorganic material, and can synchronously trigger obvious fluorescence signals and color changes in the same reaction system, so that the fluorescence-colorimetric dual-mode detection of epinephrine is realized. According to the technical scheme, the defects of poor stability and high cost of natural enzyme are avoided, the problems of tedious preparation steps, unstable performance and the like of the traditional composite fluorescent nano-enzyme are solved, meanwhile, the defects that single-mode detection is easily interfered by the environment and is insufficient in accuracy are overcome, and the method has the advantages of being simple and convenient to operate, high in sensitivity, good in accuracy and the like; the method can be used for various scenes from on-site rapid screening to laboratory accurate analysis and the like.

The present application relates to a kind of wide-range lead ion colorimetric detection method based on four manganese oxide laccase activity anti-competitive inhibition, it is related to lead ion colorimetric detection technical field.The method utilizes the activity change of Mn3O4 catalysis 2,4-dichlorophenol and 4-aminoantipyrine color reaction as signal output, when Pb (II) exists in system, color reaction is inhibited, the absorbance of solution at 510 nm is significantly reduced;The detection range of the method to Pb (II) covers 1~2000 μM, the detection limit in low concentration interval is as low as 0.31 μM, in high concentration interval still maintains good linear response, the detection range is far more than most of the colorimetric detection methods currently reported, and simultaneously, the method has excellent selectivity and anti-interference to Pb (II).

The invention provides a ganoderma lucidum fungus grass substitute cultivation medium. The ganoderma lucidum fungus grass substitute cultivation medium is prepared from, by weight, 40%-50% of oasis No.1 fungus grass powder, 9%-12% of bran, 0.5%-1.0% of gypsum powder, 6%-8% of CuSO4 and 30%-40% of water. The invention further provides a method for preparing the ganoderma lucidum pennisetum substitute cultivation medium. Compared with a conventional wood chip substitute cultivation formula, the ganoderma lucidum grass substitute cultivation medium can improve the activity of laccase secreted in the medium during ganoderma lucidum cultivation, promote degradation of lignocellulose in the medium, further improve the total polysaccharide content and the active polysaccharide content in ganoderma lucidum sporocarp and effectively improve the quality of ganoderma lucidum sporocarp raw materials.

The application belongs to the field of material chemistry, and particularly relates to a chiral manganese dioxide nanomzyme with high laccase activity and high selectivity, and a preparation method and application thereof. The application mainly comprises the following aspects: synthesis of the chiral manganese dioxide nanomzyme and optimization of the synthesis conditions, a 2,4-dichlorophenol (2,4-DP) degradation method: using the laccase-mimicking property of the chiral manganese dioxide nanomzyme to catalyze 2,4-dichlorophenol, and selective catalysis of chiral substrates: using the selective catalysis of the chiral manganese dioxide nanomzyme on chiral substrates L-DOPA and D-DOPA. The application provides a synthesis method of a biomimetic laccase with a catalytic efficiency 20 times higher than that of natural laccase and a chiral manganese dioxide nanomzyme with selective catalysis, and the biomimetic laccase and the chiral manganese dioxide nanomzyme have good application prospects.

The application provides a kind of succession degeneration auricularia auricula-judae strain species recovery method and application, belong to edible mushroom cultivation technical field.Activated succession degeneration auricularia auricula-judae strain is inoculated in poor sawdust culture medium 18~20 DEG C cultivation, the mycelium of cultivation is inoculated in poor PDA culture medium 20~22 DEG C cultivation, the mycelium of cultivation is inoculated in sawdust culture medium 22~25 DEG C cultivation, the mycelium of cultivation is inoculated in PDA culture medium 22~25 DEG C cultivation;The mycelium of cultivation is inoculated in conventional sawdust culture medium 25~28 DEG C cultivation, the mycelium obtained is inoculated in cPDA culture medium 25~28 DEG C cultivation, and the auricularia auricula-judae strain of species recovery is obtained.The method of the application can restore the species of auricularia auricula-judae strain caused by multiple succession degeneration, and the mycelium length speed, amylase activity, laccase activity, cellulase activity, single bag yield and fruit body sensory evaluation score of auricularia auricula-judae strain can be restored to consistent with the starting strain.

The invention provides a CeMOF-on-CuMOF nano enzyme, a preparation method thereof and application of the CeMOF-on-CuMOF nano enzyme in alpha-glucosidase detection, and belongs to the technical field of alpha-glucosidase detection. The MOF-on-MOF nano-enzyme (CeMOF-on-CuMOF) with double-enzyme activity is successfully synthesized, oxidase-like activity and laccase-like activity of the MOF-on-MOF nano-enzyme are both synergistically improved, a fluorescence and colorimetric dual-mode sensing method is established based on the enhanced double-enzyme activity, high-sensitivity detection of alpha-glucosidase is realized, and the detection limits are respectively as low as 0.35 U / L and 0.16 U / L. The dual-mode detection has a built-in self-correction function, so that the dual-mode detection is more reliable than the single-mode detection; and the dual-mode sensing technology based on the multifunctional MOF has a wide development prospect by virtue of the synergistic effect, the convenient multifunctional MOF synthesis method and the flexible multi-mode sensing design.

The application belongs to the technical field of microorganisms, and particularly relates to a laccase-producing Serratia marcescens and application thereof. Specifically, the application provides a high-yield laccase-producing Serratia marcescens ZH-5. It is verified through experiments that the Serratia marcescens obtained in the application has significantly improved laccase activity, reaching 16822 U / L, in a LB culture medium added with glycerol. Meanwhile, the strain has good salt tolerance and acid and alkali tolerance, heavy metal tolerance, and the laccase produced by the strain still has certain enzyme activity at 80 DEG C, and has good enzyme activity in a strong acidic environment, thus having good practical application value.

The invention discloses a nano-enzyme with laccase activity and coexistence of Cu single atoms and Cu nano-particles. The nano-enzyme is used for degrading aflatoxin B1. Cu nanoparticles are loaded on Cu single atoms through a one-step pyrolysis method, CuSAC and CuNPs are successfully synthesized, under the low concentration of the material, the CuSAC and CuNPs can rapidly generate free radicals to oxidize AFB1, the degradation rate of aflatoxin B1 within 30 min can reach 90% or above, meanwhile, the material has good anti-interference performance and stability, and experiments show that the CuSAC and CuNPs nano-enzyme has huge potential in the aspect of degrading aflatoxin B1.

This invention discloses a bimetallic co-doped carbon-based nanozyme, its preparation method, and its applications, belonging to the fields of nanomaterials and biomimetic enzyme technology. Using tannic acid and melamine as carbon and nitrogen sources, and introducing iron and manganese salts, this invention successfully prepared an iron-manganese bimetallic co-doped carbon-based nanozyme through a simple mixing, evaporation, and high-temperature calcination method. The resulting nanozyme exhibits an ultrathin nanosheet structure, with Fe and Mn elements uniformly dispersed within the carbon framework. This nanozyme demonstrates significantly enhanced laccase-like activity, with a specific activity reaching 3.4 U / mg, 6.8 times that of natural laccase from Trametes versicolor, and exhibits excellent pH and temperature stability as well as high substrate affinity. The preparation method of this invention is simple, low-cost, and easily scalable. This bimetallic co-doped carbon-based nanozyme has broad application prospects in the fields of catalytic degradation and colorimetric detection and analysis of organic pollutants.

The invention discloses a method for improving enzyme activity of laccase produced by ganoderma lucidum, aims to improve laccase production capacity of ganoderma lucidum, and belongs to the technical field of microbial ferment.The method includes the steps that dominant strains are selected in the mode that strains are screened from all stages for fermentation enzyme production, so that the enzyme activity of laccase used for follow-up production is improved, and the yield of laccase is improved. The method mainly comprises the following steps: (1) screening preservation bacteria; (2) strain recovery and screening; (3) strain amplification culture and screening; and (4) screening strain fermentation enzyme production seed liquid. According to the method, the ganoderma lucidum is screened in different stages, so that the ganoderma lucidum in each stage has the best performance, and the final laccase activity is improved. The enzyme activity of the ganoderma lucidum laccase is improved, the stability of the ganoderma lucidum laccase can be improved, and it is ensured that the enzyme activity of each batch can range from 140000 u / L to 160000 u / L.

The application discloses a kind of quaternary ammonium chitosan modified copper-based simulation laccase detection spermine and spermidine method.The detection method uses quaternary ammonium chitosan (QCS) modified poly-γ-glutamic acid copper (Cu-γ-PGA) composite nanometer enzyme as core sensing material, first by drawing spermine absorbance standard curve and spermidine absorbance standard curve, then according to spermine absorbance standard curve and spermidine absorbance standard curve, linear regression equation of the concentration and absorbance value of spermine and spermidine is constructed, according to linear regression equation, the sample to be measured containing spermine and spermidine is detected, and the concentration of spermine and spermidine contained in the sample to be measured is determined.The application first applies the simulation laccase activity of QCS functionalized copper-based nanometer enzyme Cu-γ-PGA@QCS to biological amine detection.In Tris-HCl buffer, spermine and spermidine can specifically enhance the laccase activity of Cu-γ-PGA@QCS nanometer enzyme by electrostatic interaction, promote the oxidation of substrate 2,4-dichlorophenol and the generation of semiquinone free radical, so as to realize rapid detection by deepening the color of reaction solution from light pink to deep pink.

The application belongs to the technical field of analysis and detection, and particularly relates to a high-activity nano-enzyme for simultaneously detecting various chlorophenols, a preparation method and application thereof. The nano-enzyme is a composite material formed by in-situ chelation of copper nitrate, adenine and hierarchical porous cerium metal organic framework HM UIO-66(Ce). The copper-adenine formed by coordination of copper nitrate and adenine is uniformly loaded on the surface or pore interface of the hierarchical porous cerium metal organic framework HM UIO-66(Ce) and forms a stable combined structure. The high-activity nano-enzyme has excellent laccase activity. Based on the difference in catalytic reaction kinetics of different chlorophenols, a colorimetric sensing array for detecting various chlorophenols is constructed, so that the recognition and analysis of various target substances are realized. The method does not need to rely on a complex electrode system or various buffer conditions, and has the characteristics of stable structure, simple operation and suitability for rapid detection.