System for co-immobilizing laccase and mediator on amino silanized magnetic oxidized graphene nano particles and preparation method thereof

A technology of aminosilanization and immobilization of laccase, which is applied in chemical instruments and methods, biochemical equipment and methods, and immobilized on or in inorganic carriers, which can solve the problems of non-reusable use, low use efficiency, and difficult separation, etc. problems, to achieve the effect of easy recycling, high utilization rate and low cost

Inactive Publication Date: 2017-05-10
NANJING UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It is used to solve the low content of free laccase and TEMPO mediator immobilized on the carrier, and the use efficiency is low. At the same time, graphene oxide can improve the electron transfer effici...

Method used

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Examples

Experimental program
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Embodiment 1

[0028] A method for preparing aminosilanized magnetic graphene oxide nanoparticles co-immobilized laccase and a mediator system, comprising the following steps:

[0029] (1) Preparation of Fe3O4 magnetic graphene oxide nanoparticles: first add 1.0 g of graphite powder, 6.0 g of potassium permanganate, 120 mL of concentrated sulfuric acid, and 13.3 mL of phosphoric acid into a 500 mL three-necked flask and mix thoroughly, and place the flask on ice Under water bath conditions, control its temperature during the mixing process to be less than 35°C. After the mixing is completed, heat to 50°C and stir for 10 h. After the reaction is completed, cool to room temperature and add 150 mL of ice water (containing 10 mL of 30% (wt) h 2 o 2 ), continue stirring for 1 h, after the reaction is complete, wash with 200 mL deionized water, 200 mL30% HCl, and 200 mL ethanol in sequence, and finally centrifuge the solution and dry it in vacuum to obtain graphene oxide; then weigh 100 mg of the...

Embodiment 2

[0035] A method for preparing aminosilanized magnetic graphene oxide nanoparticles co-immobilized laccase and a mediator system, comprising the following steps:

[0036] (1) Preparation of Fe3O4 magnetic graphene oxide nanoparticles: first add 1.0 g of graphite powder, 6.0 g of potassium permanganate, 120 mL of concentrated sulfuric acid, and 13.3 mL of phosphoric acid into a 500 mL three-necked flask and mix thoroughly, and place the flask on ice Under the condition of water bath, control its temperature during the mixing process to less than 35 °C. After the mixing is completed, heat to 50 °C and stir for 10 h. After the reaction is completed, cool to room temperature and add 150 mL of ice water (containing 10 mL of 30% H 2 o 2 ), continue to stir for 1 h, after the reaction is complete, wash with 200 mL of deionized water, 200 mL of 30% HCl, and 200 mL of ethanol in sequence, and finally centrifuge the solution and dry it in vacuum to obtain graphene oxide; then weigh 100 m...

Embodiment 3

[0042] A method for preparing aminosilanized magnetic graphene oxide nanoparticles co-immobilized laccase and a mediator system, comprising the following steps:

[0043] (1) Preparation of Fe3O4 magnetic graphene oxide nanoparticles: first add 1.0 g of graphite powder, 6.0 g of potassium permanganate, 120 mL of concentrated sulfuric acid, and 13.3 mL of phosphoric acid into a 500 mL three-necked flask and mix thoroughly, and place the flask on ice Under the condition of water bath, control its temperature during the mixing process to less than 35 °C. After the mixing is completed, heat to 50 °C and stir for 10 h. After the reaction is completed, cool to room temperature and add 150 mL of ice water (containing 10 mL of 30% H 2 o 2 ), continue to stir for 1 h, after the reaction is complete, wash with 200 mL of deionized water, 200 mL of 30% HCl, and 200 mL of ethanol in sequence, and finally centrifuge the solution and dry it in vacuum to obtain graphene oxide; then weigh 100 m...

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PUM

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Abstract

The invention discloses a system for co-immobilizing laccase and a mediator on amino silanized magnetic oxidized graphene nano particles and a preparation method thereof. The system is obtained by co-immobilizing the laccase and the mediator on the amino silanized magnetic oxidized graphene nano particles, 0.3-0.6 mmol of mediator is fixed in every 1 g of laccase and mediator co-immobilizing system, and the laccase activity after co-immobilization is 120-300 U/g. The laccase and the mediator are co-immobilized on the magnetic oxidized graphene carrier for the first time and can be recovered and recycled through an external magnetic field, the electron transfer efficiency between the laccase and a substrate is improved through graphite oxide, accordingly the laccase utilization rate is improved, and the cost is reduced.

Description

technical field [0001] The invention belongs to the field of preparing immobilized enzymes, in particular to an aminosilanized magnetic graphene oxide nanoparticle co-immobilized laccase and mediator system and a preparation method thereof. Background technique [0002] With the rapid development of the economy, the pollution of the water environment is becoming more and more serious. The water pollution caused by the accumulation of organic pollutants has seriously endangered the health of the ecology and the sustainable development of human beings. One of the causes of water pollution is the discharge of printing and dyeing wastewater in the dye industry, which has become the most harmful and difficult to control important pollution source. Currently used dyes mainly include azo, anthraquinone, indigo and triphenylmethane dyes, these dyes have low biodegradability, and are mutagenic and carcinogenic. Compared with traditional methods, biodegradable dyes have a stable effe...

Claims

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Application Information

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IPC IPC(8): C12N11/14C12N9/02C02F3/34C02F101/30
CPCC12N9/0061C02F3/342C02F2003/001C02F2101/308C02F2305/08C12N11/14C12Y110/03002
Inventor 贾红华夏永洋高振韦萍周华
Owner NANJING UNIV OF TECH
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