The invention discloses a double-strain co-immobilization fermented mulberry wine and a preparation method thereof. The preparation method comprises the following steps: selecting mulberries, cleaning, juicing, carrying out enzymolysis, filtering and centrifugating, regulating the components, pasteurizing, cooling, carrying out primary fermentation on immobilized Saccharomyces cerevisiae and Zygosaccharomyces cidri, clarifying, filtering, carrying out secondary fermentation, aging, filtering, purifying through an ultrafiltration membrane, and filling to obtain the finished product. The method can effectively solve problem of low storability of massive mulberries in the ripening season, and the problems of complex operation, high inactivation tendency of the strains and the like in the traditional fermented fruit wine. The method can perform mass continuous production on the mulberries, shorten the production cycle, enhance the productivity, recycle the strains and lower the production cost. The mulberry wine is clear and transparent, has the stable purple color, has the particular subtle fragrance of the mulberries, and is soft and thick in mouthfeel. Compared with the mulberry wine fermented by the traditional process, the odorous constituents are obviously enhanced. The mulberry wine has the health-care effects of tonifying the blood, nourishing yin, moisturizing the intestines, postponing aging, blackening the hair, improving the eyesight, improving the skin blood supply and the like.