Method for extracting myricetin through microbial fermentation

Abstract

The invention belongs to the technical field of separation of natural products, and relates to a method for extracting myricetin through microbial fermentation. The method comprises the following steps of (1) preparing co-immobilization gel beads containing aspergillus niger spore suspension and lactobacilli suspension; (2) mashing myricetin extraction raw materials, adding ethanol, performing reflow extraction for 1-5 times, combining filtrate, performing concentration, and drying filter residues; (3) adding water to a fermenter, adding the co-immobilization gel beads obtained in the step (1), an extract obtained in the step (2) and 1 / 5-4 / 5 of dried filter residues, and performing fermentation and extraction at 40-50 DEG C for 140-170h; (4) after the fermentation is completed, filtratingfermentation liquid with a screen mesh, and removing the co-immobilization gel beads; (5) performing reflow extraction on the filtered fermentation liquid with ethanol for 1-5 times, combining filtrate, and performing concentration so as to obtain precipitate; and (6) repeatedly washing the precipitate with water for 4-6 times so as to obtain finished products. Through the adoption of the extraction method, the yield and the purity of the myricetin are greatly increased, and the method is environmentally-friendly and safe.

Description

technical field

[0001] The invention belongs to the technical field of natural product separation, and relates to a method for extracting myricetin by microbial fermentation. Background technique

[0002] Myricetin [3,5,7-trihydroxy-2-(3,4,5-trihydroxyphenyl)-4H-1-benzofuran-4-one, Myricetin] also known as myricetin, myricetin , myricetin, myricetin, is a polyhydroxy flavonol compound, yellow needle crystal, melting point is 324.0 ~ 325.5 ℃, easily soluble in methanol, ethanol, acetone, ethyl acetate, slightly soluble in water, hardly soluble in Chloroform, petroleum ether. Modern pharmacological studies have shown that myricetin has various pharmacological activities such as anti-inflammatory and analgesic, anti-tumor, hypoglycemic, liver protection and anti-oxidation, especially in the prevention and treatment of cardiovascular diseases. In view of its excellent antioxidant function and cholesterol-lowering effects, extracting myricetin from plants has become a research ...