29results about How to "Stable fermentation" patented technology

The invention discloses a method for dynamically adjusting a tea fermentation environment by utilizing fuzzy logic control. The method comprises the following steps: S1, collecting and obtaining environment parameter data of each layer in real time; s2, fuzzy processing is carried out on the temperature, humidity and oxygen concentration in each time period through a fuzzy logic controller, and fuzzy control output of each time period is generated; s3, combining a hybrid neural network architecture and a meta-learning technology to construct an environment parameter change model; s4, optimizing the fuzzy rule base by using the environment parameter change model; s5, converting the fuzzy control output of each time period into a control instruction; s6, optimizing the fermentation environment through a cross-layer parameter equalization strategy to obtain the optimal state of the environmental parameters in the fermentation process; and S7, displaying environmental parameters of each spatial level and time stage in real time, and supporting switching between a manual mode and an automatic mode. The method combines fuzzy logic control and a deep learning technology, realizes accurate dynamic adjustment of the tea fermentation environment, and has the advantages of high adaptability and the like.

The invention discloses a tea fertilizer preparation method based on microbial fermentation, and particularly relates to the field of tea compost, tea waste is crushed and wetted, and a nitrogen source and trace elements are added to optimize the carbon nitrogen ratio, so that a foundation is laid for the fermentation process; then, selected microbial strains are proliferated and purified, and the treated waste materials are uniformly inoculated with the selected microbial strains; in the fermentation process, the stability of fermentation conditions is ensured by controlling the temperature and the humidity; the tannin concentration and the pH value are monitored in real time, when the tannin concentration exceeds a preset value and the pH deviates from a normal range, the abnormal state of tannin degradation is evaluated and identified through a reaction activity index, an interference source is positioned through a sparse principal component analysis algorithm, and noise signals are removed; and after the interference source is positioned, the fermentation condition is adjusted in a targeted manner, so that the tannin degradation efficiency reaches the expectation, and the compost is maturely fermented and meets the fertilizer use standard.

The invention discloses a fruity yoghurt powder formula and a preparation method thereof, and relates to the technical field of food processing.The fruity yoghurt powder formula comprises milk, white granulated sugar, fruit juice, a yoghurt leavening agent, a stabilizer, a sweetening agent, an emulsifying agent, an acidity adjusting agent and water, the stabilizer is one of modified starch, gelatin, pectin and a yoghourt modifier. The stabilizer is added to improve the texture and stability of the yoghurt and reduce the problems of whey separation, texture roughness and the like, so that the formula of the fruity yoghurt powder is optimized, the taste can be ensured, the stability and quality of the yoghurt are enhanced, the layering or precipitation phenomenon of the yoghurt in the storage and transportation process is prevented, and the quality of the yoghurt is improved. Streptococcus thermophilus and lactobacillus bulgaricus in a mass ratio of 2: 1 are selected as yoghurt leavening agents, so that the fermentation process is stable, and the yoghurt is fine and smooth in texture.

The invention discloses a household garbage undersize humus resourceful treatment system and method, and belongs to the technical field of solid waste treatment. Comprising the following steps: mounting an aeration pipeline in a bottom groove of a fermentation unit of the humus soil recycling site, and connecting the aeration pipeline with a fan system outside the fermentation unit; a broken stone hardcore and an isolating layer are sequentially arranged on the aeration pipeline of the fermentation unit; humus soil which is uniformly mixed with a high-temperature fermentation inoculant is contained on the isolation layer in the fermentation unit, and the humus soil is covered with a high-molecular intelligent film; the humus soil is subjected to automatic fermentation control, so that resourceful treatment of the humus soil is realized. According to the method, the exclusive high-temperature fermentation inoculant is added into the humus soil, and organic matters in the humus soil can be stably, efficiently and environment-friendly removed by adopting a high-molecular intelligent membrane automatic fermentation technology, so that the treated humus soil meets the engineering construction backfill soil requirements, and the tail-section treatment problem of the screened humus soil can be thoroughly solved; the method has popularization significance in the field of stock household garbage disposal.

This utility model discloses a sterilization tank device for probiotic fermented beverages, including a container mechanism and a cleaning mechanism. The container mechanism includes a tank body, a lid that snaps onto the top of the tank body, and a high-temperature pipe penetrating the top of the lid. The cleaning mechanism involves cleaning the inside of the tank by re-closing the lid, introducing clean water and detergent through the high-temperature pipe, activating a hydraulic cylinder, and driving a transmission rod to reciprocate, causing an extension plate and sponges on the plate to repeatedly squeeze the inner wall of the tank. The porous structure of the sponges facilitates air entry, thus better generating foam. Once sufficient foam is generated, the hydraulic cylinder is closed and the motor is started, causing the two sponges to rotate synchronously. The two sponges work together to effectively clean the inner wall of the tank. The lid is then opened to drain the foamy water from the tank. This cleaning process is repeated three times to ensure thorough removal of residual foam.

This invention belongs to the fields of fermentation engineering and enzyme engineering technology, specifically relating to a chitosanase fermentation method based on a Pichia pastoris expression vector and its application. The chitosanase fermentation method provided by this invention includes two stages: fermentation during the cell growth phase and fermentation during the induction phase, with strict control over the carbon source and specific feeding method at each stage. During fermentation, this invention significantly improves the expression level and absolute enzyme activity of chitosanase Baa by adding casein hydrolysate as a molecular chaperone, mannitol as an adjuvant, and further optimizing the carbon source feeding strategy and parameters such as pH and temperature. Compared with the starting process, this invention increases the chitosanase activity by 1.81 times and the protein expression level by 2.01 times. Moreover, the fermentation method of this invention has been successfully verified in a 50L pilot-scale operation, demonstrating a good foundation for industrial application and cost advantages.

This invention discloses a harmless treatment device for livestock and poultry breeding waste, belonging to the field of anaerobic treatment technology. To address the problem of biogas suffocation during biogas digester use, leading to a significant reduction in effective gas production and affecting digester safety, as well as poor gas-liquid separation during gas collection and severe liquid contamination in biogas, resulting in low pure gas capture rates, the invention includes a biogas digester body and an inlet connected to one side of the digester body. A skylight cover is fixed to the upper center of the digester body, and ventilation ports are provided on both sides of the bottom surface of the skylight cover. This invention enables the collection and circulation of biogas from within the liquid. Combined with the ventilation ports and guide pipes, it collects and circulates the naturally rising biogas within the digester body, improving biogas collection efficiency while preventing scum and foam from covering the gas-liquid interface, thus avoiding suffocation of biogas and affecting biogas collection efficiency and pressure safety within the digester body.

This invention provides an engineered bacterium for producing ectoine, its preparation method, and its application. The engineered bacterium is prepared using a method comprising the following steps: using *Corynebacterium glutamicum* as the starting strain, feedback inhibition by aspartate kinase LysC is relieved, the activity of the gene lysE encoding lysine efflux permease is reduced, and the ectoine synthesis gene cluster ectABC is expressed. Based on this, the activity of any one or at least two of the following—diaminobutyrate acetyltransferase EctA, pyruvate carboxylase Pyc, or aspartate kinase LysC—is enhanced, and / or the activity of any one or at least two of the following—maloquinone oxidoreductase Mqo or homoserine dehydrogenase Hom—is reduced to further increase ectoine yield.

The utility model belongs to the technical field of plant beverage production equipment, and discloses a fermentation device for plant beverage production for controlling blood sugar, which comprises a fermentation tank, an outer-layer temperature control component arranged in the tank wall of the fermentation tank, an inner-layer temperature control component arranged in a ring shape in the fermentation tank, an inner-layer stirring component arranged on the inner side of the inner-layer temperature control component, and an outer-layer stirring component arranged on the inner side of the inner-layer stirring component, an outer-layer stirring assembly is arranged on the outer side of the inner-layer temperature control assembly, the inner-layer stirring assembly and the outer-layer stirring assembly are jointly connected with a rotary driving mechanism, an oxygenation structure is further arranged in the fermentation tank, and a vacuum pump is installed on the tank wall of the fermentation tank; the fermentation device is simple in overall structure, can be used for both aerobic fermentation and anaerobic fermentation, improves the applicability, and ensures the fermentation quality and the fermentation efficiency of the fermentation device.

The application discloses collagen tripeptide and a preparation method and application thereof, and belongs to the technical field of bioengineering. The preparation method comprises the following steps: obtaining bacillus velezensis with high collagenase yield through separation and identification, the bacillus velezensis belongs to probiotics, and high-activity collagenase is obtained through fermentation culture and purification; taking shark cartilage or blue fish skin as raw materials, and obtaining collagen tripeptide through the following steps of combined enzymolysis of alkaline protease and collagenase after pretreatment, inactivation, centrifugal separation and freeze-drying. The collagen tripeptide prepared by the application has a molecular weight of less than 500 Dalton, contains active components such as GHK tripeptide, is easy to absorb and has high safety, and can be used for preparing antioxidant products, anti-inflammatory products, memory-enhancing products or immunity-enhancing products. The application solves the problems of unsafe collagenase source and low collagen tripeptide preparation efficiency, and has a good industrial application prospect.

The invention relates to the technical field of agriculture, in particular to a method for fusing anaerobic fermentation compost, which comprises the following steps: mixing organic waste raw materials, and adding sodium citrate and magnesium sulfate for treatment; feeding the treated raw materials into an anaerobic reactor, adding an iron-based core-shell structure material, controlling the pH value of the system, and carrying out anaerobic fermentation to obtain digestion residues; bicarbonate and sodium citrate are added into the digestion residues to be mixed; and entering an aerobic composting stage, controlling the moisture content of the pile body under an oxygen supply condition, heating the pile body, maintaining the pile body to enter a decomposition stage, and carrying out natural aging to produce a decomposed organic fertilizer. In the invention, by virtue of the porous structure and surface characteristics of the iron-based core-shell structure material, not only is the stability of the fermentation process ensured, but also the microbial electron transfer process is optimized, and the methane production efficiency is improved; the compound microbial agent effectively increases the number of effective microbial communities, improves the temperature and ventilation conditions in the composting process, and ensures uniform decomposition of the organic wastes.

The invention relates to the technical field of quality prediction, in particular to a fermentation quality prediction management system for enzyme production based on data processing. The system comprises the following modules: an environmental parameter acquisition module, a microbial activity monitoring module, a fermentation state identification module and a prediction regulation and control module, wherein the environmental parameter acquisition module acquires environmental condition parameters in a fermentation tank in real time through a multi-parameter sensor array; an enzyme activity index and nutrition consumption rate data are obtained from the microbial activity monitoring module; the microbial activity monitoring module is used for judging a microbial behavior track, correcting track offset and identifying characteristics of each fermentation stage; and the prediction regulation and control module predicts the product quality, and optimizes fermentation process parameters according to the deviation between the prediction result and the target quality. According to the method, prospective control over the enzyme production quality is achieved, the intelligent level of the whole fermentation process is improved, and a flexible and efficient fermentation quality control mechanism is formed.

The invention discloses a biological organic fertilizer fermentation device, and relates to the technical field of fertilizer ferment.The biological organic fertilizer fermentation device comprises a fermentation box body, a mounting box is arranged in the fermentation box body, a connecting column is rotationally connected to the outer wall of the bottom of the mounting box, and the connecting column is driven to rotate through a servo motor; the turning paddle turns over compost in the middle of the fermentation box, cranks at the two ends are driven by the transmission column to rotate, the cleaning column is promoted to swing in a reciprocating mode, the inner side edge of the fermentation box is turned over through the cleaning column on the lower portion of the cleaning column, and the inner wall of the fermentation box can be cleaned through the cleaning brush on the cleaning column. Therefore, the problem of material accumulation caused by pile turning dead angles in the pile turning process can be avoided, uniform distribution of the materials in a pile body can be ensured, microorganisms can be in full contact with each part of the materials, more uniform fermentation is further realized, decomposition of organic matters and release of nutrients are accelerated, and the fermentation period is shortened.

The invention relates to the field of microbial fermentation, in particular to a device and a method for directly fermenting fresh rhizoma polygonati by using edible mushrooms. Comprising a box body, a treatment cavity and a sterilization cavity are formed in the box body, a sealing door is installed on the front side of the treatment cavity, and a control switch and a display panel are installed on the front side of the box body; and the placement mechanism comprises a first rectangular groove formed in the inner bottom of the treatment cavity, a bearing plate capable of sliding left and right is arranged in the first rectangular groove, a second rectangular groove is formed in the upper end of the bearing plate, and a shaking plate capable of sliding up and down is arranged in the second rectangular groove. Compared with a preparation method in the prior art, the fermentation method is simple, safe and wide in raw material source, the prepared fermentation product has a very good anti-tumor effect, in addition, in the fermentation device, the solid fermentation product can be subjected to shaking and gas injection treatment, solid fermentation is promoted for aerobic respiration, and the fermentation efficiency and effect are improved.

The roxburgh rose pomace fermentation device comprises a cuboid-shaped fermentation tank, a fermentation cavity formed by a metal groove body is formed in the fermentation tank, and the fermentation cavity is provided with an opening distributed upwards; a heating plate is arranged between the metal tank body and the fermentation tank, the inner surface of the heating plate is in contact with the metal tank body, and a heat insulation layer is arranged on the outer surface of the heating plate; a sealing cover for covering the fermentation cavity is arranged at the top of the fermentation tank and is provided with an exhaust valve; a plurality of positioning convex columns are arranged at the top of the sealing cover, and positioning grooves matched with the positioning convex columns in an inserting manner are correspondingly formed in the bottom of the fermentation tank. According to the utility model, the fermentation tank is designed into a cuboid shape, so that the fermentation tank is conveniently and stably stacked and placed, and the storage space occupied during fermentation is reduced; through the matching of the positioning convex columns and the positioning grooves, the fermentation tanks which are stacked up and down are relatively stable and are not easy to topple and overturn; in addition, proper heating and temperature rise can be achieved, and temperature fermentation is kept.

The utility model belongs to the technical field of fermentation production equipment, and particularly relates to a constant temperature device for a fermentation tank, which comprises a fermentation tank body, two electric heating wires are fixedly connected to the side wall of the fermentation tank body, the two electric heating wires are oppositely arranged, two water pumps are fixedly connected to the side wall of the fermentation tank body, the two water pumps are oppositely arranged, water inlet pipes are fixedly connected to the side walls of the water pumps, water storage pipes are fixedly connected to the bottoms of the water pumps, and the two water storage pipes are fixedly connected between the two electric heating wires. The temperature outside the fermentation tank body is controlled and transmitted to the interior of the fermentation tank body, heating and cooling effects can be achieved under the condition that the temperature is unstable, the temperature inside the fermentation tank body can be kept stable and balanced through cooperative use of the electric heating wire and the water storage pipe, and the fermentation tank is convenient to use. Therefore, a constant-temperature fermentation environment can be effectively provided, and energy can be effectively utilized through the electric heating wire and the water storage pipe.

The invention relates to the technical field of organic fertilizer production, in particular to an organic fertilizer production process and equipment based on three-source fermentation, and the process comprises the following steps: crushing, screening and mixing fermentation raw materials to obtain a fermentation base material; stirring and mixing the fermentation base material, water and the fermentation culture medium additive to obtain a fermentation mixed matrix; stirring the fermentation mixed matrix for fermentation, obtaining the mixed gas production speed of mixed gas generated in the stirring process in the detection period to adjust the fermentation temperature in the fermentation tank, and detecting the microbial concentration to obtain the actual microbial concentration; the fermentation stirring speed is adjusted to meet the fermentation requirement under the condition that the microbial concentration cannot meet the fermentation requirement; whether fermentation is stopped or not is judged by detecting oxygen consumption and fermentation temperature changes, and the fermented mixed substrate is guided into an organic fertilizer separation device to obtain the organic fertilizer. By accurately controlling the particle size of the fermentation base material and adjusting the fermentation temperature, the microbial activity is improved, and the quality of the organic fertilizer is improved.

The invention provides a food fermentation control method, a refrigerator and a computer readable storage medium. The food fermentation control method comprises the following steps: acquiring a target oxygen concentration range corresponding to food to be fermented; and the electrochemical module is controlled to generate oxygen through electrochemical reaction, and the oxygen is discharged into the refrigeration box body, so that the current oxygen concentration in the refrigeration box body is within the target oxygen concentration range. The cold storage box body adopts a sealed direct cooling mode, so that a low-temperature and high-humidity environment can be provided; in addition, the oxygen content in the refrigeration space can be increased by carrying the electrochemical module, so that a high-oxygen environment can be provided; therefore, fermentation can be performed under high-oxygen, low-temperature and high-humidity conditions, food flavor can be more unique, food aging can be delayed, the fermentation process is more stable and controllable, and the problems that food is easy to become sour due to too fast normal-temperature fermentation, low-temperature fermentation is too slow, the flavor is not faint scent and the like can be solved; the activity of low-temperature fermentation yeast of the food is effectively promoted; the unique flavor formation of the fermented food is improved.

The all-dimensional turning and throwing device for the dynamic composting of the agricultural product waste organic fertilizer comprises a composting pool, an adjustable turning structure and a gathering structure are arranged on the composting pool, and limiting sliding grooves and a plurality of groups of positioning columns are symmetrically arranged on the composting pool. By adopting the structure, the compost material is conveyed upwards and continuously turned, so that the material is ventilated and cooled and is fully contacted and reacted with oxygen, and stable fermentation of organic matters in the material is ensured; bottom guiding, material gathering adjustment and matched material conveying and turning are achieved, a closed loop is formed, automatic machining is achieved, manual operation is avoided, and safety and high efficiency are achieved. The device is simple and reasonable in overall structure, convenient to operate and use and high in automation degree.

The present application relates to the technical field of wine brewing, in particular to a method for brewing Thompson Seedless white grape wine by carbon dioxide impregnation fermentation, comprising the following steps: adopting a process scheme combining carbon dioxide whole fruit impregnation and sequential inoculation of multiple yeasts, placing whole Thompson Seedless white grape berries in a closed carbon dioxide environment for impregnation at 28-32 DEG C for 8-12 days to promote the dissolution and conversion of skin aroma precursors; after crushing, juice extraction and clarification, first inoculating non-wine yeast such as Cryptococcus or Saccharomycodes deemii for fermentation for 48 hours, and then inoculating wine yeast QA23 or EC1118 to complete main fermentation and segmented temperature control. The present application significantly increases the content of ester and terpene aroma substances, enhances fruitiness and floral characteristics, effectively improves the complexity and level of aroma, guarantees fermentation stability, and provides a new technical approach for the quality upgrading of Thompson Seedless white grape wine.

This invention belongs to the field of tea processing technology. It provides a novel comprehensive processing technique for improving the quality of summer and autumn black tea, comprising the steps of gentle shaking combined with UV-B-LED combined spectral withering, low-temperature controlled pressure gradient kneading, three-parameter linked dynamic fermentation, microwave-hot air coupled drying, and far-infrared variable-temperature aroma enhancement. Gentle shaking combined with UV-B-LED combined spectroscopy promotes the polymerization and transformation of flavor components; low-temperature controlled pressure gradient kneading allows for controllable cell breakage of tea leaves, promoting the outflow of tea juice; three-parameter linked dynamic fermentation increases the content of theaflavins and aromatic substances, improving the uniformity of fermentation; microwave-hot air coupled drying reduces aroma loss and improves drying efficiency; far-infrared variable-temperature aroma enhancement enriches the aroma of the tea, improves tea quality, and enhances the harmony and lasting sweetness of the tea soup. The resulting black tea has a complex aroma of flowers and honey, a bright red liquor, a mellow and refreshing taste, and a comprehensive sensory score as high as 95.6.

The present invention discloses a method for dynamically adjusting the environment of tea fermentation using fuzzy logic control, comprising the following steps: S1, real-time collection and acquisition of environmental parameter data of each layer; S2, fuzzy processing of temperature, humidity and oxygen concentration in each time period by a fuzzy logic controller, generating fuzzy control outputs of each time period; S3, combining a hybrid neural network architecture with meta-learning technology to construct an environmental parameter change model; S4, optimizing the fuzzy rule base using the environmental parameter change model; S5, converting the fuzzy control outputs of each time period into control instructions; S6, optimizing the fermentation environment through a cross-layer parameter balancing strategy, and obtaining the optimal state of environmental parameters of the fermentation process; S7, real-time display of environmental parameters of each spatial level and time stage, and supporting manual and automatic mode switching. The present invention combines fuzzy logic control with deep learning technology to achieve accurate dynamic adjustment of the tea fermentation environment, and has the advantages of strong adaptability.

The utility model provides bean product processing and fermenting equipment. The bean product processing and fermenting equipment comprises a box body, a fermentation cavity is formed in the box body, multiple sets of placement parts are oppositely arranged on the two opposite inner side faces of the fermentation cavity, fermentation discs are arranged on the multiple sets of placement parts in a replaceable mode, and breathable pads are arranged in the multiple sets of fermentation discs; two groups of assembly parts are oppositely arranged on the box body close to one side of the fermentation cavity, and a protective door assembly is rotatably arranged on the two groups of assembly parts; a temperature and humidity control assembly is further arranged on one side of the box body. The temperature sensor and the humidity sensor can continuously collect temperature data in the fermentation cavity, the data can be instantly transmitted to the display panel to be clearly displayed, and when the monitored temperature or humidity deviates from a preset standard value, the heating plate can start the heating function according to the temperature condition so as to recover the proper temperature. And the steam humidifier can perform spraying operation according to the humidity requirement to adjust the humidity, so that the stable proceeding of the bean product fermentation process is guaranteed.

The invention discloses a covering aerobic high-temperature fermentation composting system based on an intelligent molecular film in the technical field of organic fertilizer fermentation, the system comprises a composting table and the molecular film, a retaining wall is arranged on the side edge of the composting table, a mounting rack is slidably connected to the retaining wall, and a winding drum for winding the molecular film is rotatably mounted on the mounting rack; a driving mechanism for driving the mounting frame to move is arranged on the retaining wall; a pressing plate is arranged above the composting table, an L-shaped first sliding groove is formed in the retaining wall, a first sliding block is installed in the first sliding groove in a sliding mode, and the first sliding block is fixedly connected with the pressing plate; a first push block and a wedge-shaped push block are arranged on the side edge of the first sliding block and fixedly connected with the mounting frame. Two groups of film pressing mechanisms are also arranged above the composting table and are symmetrically arranged relative to the pressing plate; according to the invention, the compost can be better fermented.

The utility model discloses a kind of quick fermentation edible mushroom processing device, it is related to edible mushroom processing technical field, including fermentation tank, fermentation tank is equipped with fermentation adjusting mechanism and oxygen feeding mechanism in its inside, fermentation adjusting mechanism includes first rotating shaft, the one end of first rotating shaft is slidably connected with second rotating shaft, the outside of second rotating shaft is fixedly connected with electric push rod, in initial fermentation process in the utility model, pinion is engaged with fixed toothing, can drive connecting shaft self-rotation, stirring blade rotates, to quickly turn animal material, promote the mixing of material in fermentation tank inside, in fermentation later period, electric push rod works and drives pinion to move and separate with fixed toothing, connecting shaft only follows the rotation of first rotating shaft and does circular motion, reduce the mechanical damage to mycelium, while maintaining good mixing effect, guarantee fermentation process stable progress, according to fermentation process switching mixing mode, improve mixing effect while reducing material damage.

The invention relates to the technical field of lactic acid bacteria fermentation, and discloses lactic acid bacteria powder fermentation culture equipment which comprises a support, a fermentation tank is arranged at the top of the support, a top cover is arranged at the top of the fermentation tank, a top pressing assembly is arranged at the bottom end of the top cover, and a fermentation cavity is formed in the middle of the fermentation tank. A partition disc is arranged on the inner wall of the fermentation cavity, a driving shaft is arranged in the middle of the partition disc, a long shaft is arranged on the outer side of the driving shaft, and a stirring assembly is arranged on the outer side of the long shaft. Lactic acid bacteria powder to be stirred is poured into the fermentation cavity, when the sealing plug is inserted into the fermentation cavity, a right-angle block arranged at the bottom end of the sealing plug abuts against a pressing rod in the compression driving part, the driving shaft is driven to drive the stirring assembly, and therefore the lactic acid bacteria powder to be fermented and the lactic acid bacteria powder being fermented are stirred; and the lactic acid bacteria powder can be mixed more uniformly and fermented better.

The present invention provides a hypoxanthine production strain and a construction method and application thereof, wherein the strain overexpresses <h2 style=";text-align:left;direction:ltr">glnA The gene encoding glutamine synthetase and <h2 style=";text-align:left;direction:ltr"> aspC Gene encoding aspartate aminotransferase, <h2 style=";text-align:left;direction:ltr"> thrA The natural promoter of the gene was replaced by P BBa_J23109 ; then <h2 style=";text-align:left;direction:ltr"> pgi The natural promoter of the gene was replaced by P BBa_J23105 , knocking out the gene encoding 6-phosphogluconate dehydratase <h2 style=";text-align:left;direction:ltr"> edd ; Finally, the output system of hypoxanthine was strengthened and the <h2 style=";text-align:left;direction:ltr"> nepI Finally, a high-yield hypoxanthine engineering strain with a clear genetic background and stable fermentation process was obtained, which could achieve a hypoxanthine yield of 32.8 g / L in a 5L fermenter within 48 hours.

The utility model discloses a persimmon vinegar fermentation culture device capable of controlling a fermentation environment, which relates to the field of persimmon vinegar fermentation and comprises a fermentation barrel body and a barrel cover movably mounted at the top of the fermentation barrel body. A stirring assembly located in the fermentation barrel body is movably installed at the bottom of the barrel cover, a placing assembly is movably installed in the fermentation barrel body, a discharge pipe is fixedly connected to the lower portion of the rear side of the fermentation barrel body, and a butterfly valve is fixedly installed outside the discharge pipe. According to the utility model, the clamping design is adopted in the fermentation barrel body, and the temperature control assembly is arranged in the fermentation barrel body, so that the temperature control assembly is matched with the stirring assembly to uniformly heat the fermentation barrel body to control the fermentation temperature in the fermentation culture process of raw materials; the temperature control assembly monitors the change of the fermentation temperature environment in real time and regulates and controls the temperature, so that stable fermentation of the persimmon vinegar is guaranteed, and the flavor and taste of the persimmon vinegar are guaranteed.