11results about How to "Stable fermentation" patented technology

This utility model discloses a sterilization tank device for probiotic fermented beverages, including a container mechanism and a cleaning mechanism. The container mechanism includes a tank body, a lid that snaps onto the top of the tank body, and a high-temperature pipe penetrating the top of the lid. The cleaning mechanism involves cleaning the inside of the tank by re-closing the lid, introducing clean water and detergent through the high-temperature pipe, activating a hydraulic cylinder, and driving a transmission rod to reciprocate, causing an extension plate and sponges on the plate to repeatedly squeeze the inner wall of the tank. The porous structure of the sponges facilitates air entry, thus better generating foam. Once sufficient foam is generated, the hydraulic cylinder is closed and the motor is started, causing the two sponges to rotate synchronously. The two sponges work together to effectively clean the inner wall of the tank. The lid is then opened to drain the foamy water from the tank. This cleaning process is repeated three times to ensure thorough removal of residual foam.

This invention belongs to the fields of fermentation engineering and enzyme engineering technology, specifically relating to a chitosanase fermentation method based on a Pichia pastoris expression vector and its application. The chitosanase fermentation method provided by this invention includes two stages: fermentation during the cell growth phase and fermentation during the induction phase, with strict control over the carbon source and specific feeding method at each stage. During fermentation, this invention significantly improves the expression level and absolute enzyme activity of chitosanase Baa by adding casein hydrolysate as a molecular chaperone, mannitol as an adjuvant, and further optimizing the carbon source feeding strategy and parameters such as pH and temperature. Compared with the starting process, this invention increases the chitosanase activity by 1.81 times and the protein expression level by 2.01 times. Moreover, the fermentation method of this invention has been successfully verified in a 50L pilot-scale operation, demonstrating a good foundation for industrial application and cost advantages.

This invention discloses a harmless treatment device for livestock and poultry breeding waste, belonging to the field of anaerobic treatment technology. To address the problem of biogas suffocation during biogas digester use, leading to a significant reduction in effective gas production and affecting digester safety, as well as poor gas-liquid separation during gas collection and severe liquid contamination in biogas, resulting in low pure gas capture rates, the invention includes a biogas digester body and an inlet connected to one side of the digester body. A skylight cover is fixed to the upper center of the digester body, and ventilation ports are provided on both sides of the bottom surface of the skylight cover. This invention enables the collection and circulation of biogas from within the liquid. Combined with the ventilation ports and guide pipes, it collects and circulates the naturally rising biogas within the digester body, improving biogas collection efficiency while preventing scum and foam from covering the gas-liquid interface, thus avoiding suffocation of biogas and affecting biogas collection efficiency and pressure safety within the digester body.

This invention provides an engineered bacterium for producing ectoine, its preparation method, and its application. The engineered bacterium is prepared using a method comprising the following steps: using *Corynebacterium glutamicum* as the starting strain, feedback inhibition by aspartate kinase LysC is relieved, the activity of the gene lysE encoding lysine efflux permease is reduced, and the ectoine synthesis gene cluster ectABC is expressed. Based on this, the activity of any one or at least two of the following—diaminobutyrate acetyltransferase EctA, pyruvate carboxylase Pyc, or aspartate kinase LysC—is enhanced, and / or the activity of any one or at least two of the following—maloquinone oxidoreductase Mqo or homoserine dehydrogenase Hom—is reduced to further increase ectoine yield.

The application discloses collagen tripeptide and a preparation method and application thereof, and belongs to the technical field of bioengineering. The preparation method comprises the following steps: obtaining bacillus velezensis with high collagenase yield through separation and identification, the bacillus velezensis belongs to probiotics, and high-activity collagenase is obtained through fermentation culture and purification; taking shark cartilage or blue fish skin as raw materials, and obtaining collagen tripeptide through the following steps of combined enzymolysis of alkaline protease and collagenase after pretreatment, inactivation, centrifugal separation and freeze-drying. The collagen tripeptide prepared by the application has a molecular weight of less than 500 Dalton, contains active components such as GHK tripeptide, is easy to absorb and has high safety, and can be used for preparing antioxidant products, anti-inflammatory products, memory-enhancing products or immunity-enhancing products. The application solves the problems of unsafe collagenase source and low collagen tripeptide preparation efficiency, and has a good industrial application prospect.

The invention relates to the technical field of agriculture, in particular to a method for fusing anaerobic fermentation compost, which comprises the following steps: mixing organic waste raw materials, and adding sodium citrate and magnesium sulfate for treatment; feeding the treated raw materials into an anaerobic reactor, adding an iron-based core-shell structure material, controlling the pH value of the system, and carrying out anaerobic fermentation to obtain digestion residues; bicarbonate and sodium citrate are added into the digestion residues to be mixed; and entering an aerobic composting stage, controlling the moisture content of the pile body under an oxygen supply condition, heating the pile body, maintaining the pile body to enter a decomposition stage, and carrying out natural aging to produce a decomposed organic fertilizer. In the invention, by virtue of the porous structure and surface characteristics of the iron-based core-shell structure material, not only is the stability of the fermentation process ensured, but also the microbial electron transfer process is optimized, and the methane production efficiency is improved; the compound microbial agent effectively increases the number of effective microbial communities, improves the temperature and ventilation conditions in the composting process, and ensures uniform decomposition of the organic wastes.

The invention relates to the technical field of quality prediction, in particular to a fermentation quality prediction management system for enzyme production based on data processing. The system comprises the following modules: an environmental parameter acquisition module, a microbial activity monitoring module, a fermentation state identification module and a prediction regulation and control module, wherein the environmental parameter acquisition module acquires environmental condition parameters in a fermentation tank in real time through a multi-parameter sensor array; an enzyme activity index and nutrition consumption rate data are obtained from the microbial activity monitoring module; the microbial activity monitoring module is used for judging a microbial behavior track, correcting track offset and identifying characteristics of each fermentation stage; and the prediction regulation and control module predicts the product quality, and optimizes fermentation process parameters according to the deviation between the prediction result and the target quality. According to the method, prospective control over the enzyme production quality is achieved, the intelligent level of the whole fermentation process is improved, and a flexible and efficient fermentation quality control mechanism is formed.

The invention relates to the field of microbial fermentation, in particular to a device and a method for directly fermenting fresh rhizoma polygonati by using edible mushrooms. Comprising a box body, a treatment cavity and a sterilization cavity are formed in the box body, a sealing door is installed on the front side of the treatment cavity, and a control switch and a display panel are installed on the front side of the box body; and the placement mechanism comprises a first rectangular groove formed in the inner bottom of the treatment cavity, a bearing plate capable of sliding left and right is arranged in the first rectangular groove, a second rectangular groove is formed in the upper end of the bearing plate, and a shaking plate capable of sliding up and down is arranged in the second rectangular groove. Compared with a preparation method in the prior art, the fermentation method is simple, safe and wide in raw material source, the prepared fermentation product has a very good anti-tumor effect, in addition, in the fermentation device, the solid fermentation product can be subjected to shaking and gas injection treatment, solid fermentation is promoted for aerobic respiration, and the fermentation efficiency and effect are improved.

This invention belongs to the field of tea processing technology. It provides a novel comprehensive processing technique for improving the quality of summer and autumn black tea, comprising the steps of gentle shaking combined with UV-B-LED combined spectral withering, low-temperature controlled pressure gradient kneading, three-parameter linked dynamic fermentation, microwave-hot air coupled drying, and far-infrared variable-temperature aroma enhancement. Gentle shaking combined with UV-B-LED combined spectroscopy promotes the polymerization and transformation of flavor components; low-temperature controlled pressure gradient kneading allows for controllable cell breakage of tea leaves, promoting the outflow of tea juice; three-parameter linked dynamic fermentation increases the content of theaflavins and aromatic substances, improving the uniformity of fermentation; microwave-hot air coupled drying reduces aroma loss and improves drying efficiency; far-infrared variable-temperature aroma enhancement enriches the aroma of the tea, improves tea quality, and enhances the harmony and lasting sweetness of the tea soup. The resulting black tea has a complex aroma of flowers and honey, a bright red liquor, a mellow and refreshing taste, and a comprehensive sensory score as high as 95.6.

The utility model provides bean product processing and fermenting equipment. The bean product processing and fermenting equipment comprises a box body, a fermentation cavity is formed in the box body, multiple sets of placement parts are oppositely arranged on the two opposite inner side faces of the fermentation cavity, fermentation discs are arranged on the multiple sets of placement parts in a replaceable mode, and breathable pads are arranged in the multiple sets of fermentation discs; two groups of assembly parts are oppositely arranged on the box body close to one side of the fermentation cavity, and a protective door assembly is rotatably arranged on the two groups of assembly parts; a temperature and humidity control assembly is further arranged on one side of the box body. The temperature sensor and the humidity sensor can continuously collect temperature data in the fermentation cavity, the data can be instantly transmitted to the display panel to be clearly displayed, and when the monitored temperature or humidity deviates from a preset standard value, the heating plate can start the heating function according to the temperature condition so as to recover the proper temperature. And the steam humidifier can perform spraying operation according to the humidity requirement to adjust the humidity, so that the stable proceeding of the bean product fermentation process is guaranteed.

The utility model discloses a kind of quick fermentation edible mushroom processing device, it is related to edible mushroom processing technical field, including fermentation tank, fermentation tank is equipped with fermentation adjusting mechanism and oxygen feeding mechanism in its inside, fermentation adjusting mechanism includes first rotating shaft, the one end of first rotating shaft is slidably connected with second rotating shaft, the outside of second rotating shaft is fixedly connected with electric push rod, in initial fermentation process in the utility model, pinion is engaged with fixed toothing, can drive connecting shaft self-rotation, stirring blade rotates, to quickly turn animal material, promote the mixing of material in fermentation tank inside, in fermentation later period, electric push rod works and drives pinion to move and separate with fixed toothing, connecting shaft only follows the rotation of first rotating shaft and does circular motion, reduce the mechanical damage to mycelium, while maintaining good mixing effect, guarantee fermentation process stable progress, according to fermentation process switching mixing mode, improve mixing effect while reducing material damage.