Patents
Literature
Patsnap Copilot is an intelligent assistant for R&D personnel, combined with Patent DNA, to facilitate innovative research.
Patsnap Copilot

352results about How to "Thoroughly sterilized" patented technology

Method for producing methane as clean energy source by utilizing catering organic waste

The invention relates to a method for producing a clean energy source by a continuous feeding type anaerobic fermentation method, particularly to a continuous feeding type anaerobic fermentation method for preparing methane by fermenting catering organic waste. The method for producing the clean energy source through continuous feeding type anaerobic fermentation can be widely used for preparing the methane which is a clean energy source through the anaerobic fermentation of the catering organic waste, straws, animal excreta in farms and other organic waste. The method comprises the following steps of: draining partial moisture of the collected catering organic waste to enable the water content thereof to be not more than 80 percent, and then adding the processed catering organic waste and acclimated anaerobic activated sludge in a definite proportion into a reactor for fermentation. The catering organic waste is added into the reactor by adopting a continuous feeding method in the fermentation process, and a fermentation raw material is added once at regular intervals. An addition period is a gas production vigorous period. The addition is 0.1-10 percent of the volume of the reactor. An anaerobic state is strictly controlled in the whole process so as to facilitate the growth of anaerobes.
Owner:BEIJING UNIV OF CHEM TECH

Box-type cultivation method of tussah pupa cordyceps militaris

The invention relates to a box-type cultivation method of tussah pupa cordyceps militaris. The method comprises the steps of tussah pupa cleaning, sterilization, inoculation and cordyceps militaris cultivation. The strain is cultivated via a PDA liquid medium; and the strain is cultivated in a table concentrator at a temperature of about 23 DEG C till the culture solution is viscous and fungus balls appear after the medium is sterilized and inoculated at a high temperature. The cultivated liquid strain is filtered to an aseptic bottle; the fungus balls are washed with sterile water for 2-3 times, and the volume of the sterile water 0.5-1 time that of the bacteria liquid; and the whole process is finished through aseptic operations. During the inoculation, the bacteria liquid is absorbed and injected at a second section from the tail of the tussah pupa via an injector; after the inoculation, a single layer of the tussah pupa is put in a sterilized culture room; after the inoculated part scabs, the tussah pupas are transferred to a sterilized plastic box for cultivation; after the tail of the tussah pupa is rigidified and hardened and mycelia appear at the section, the tussah pupa is put in a box with air vents; the temperature and the humidity are kept till cordyceps militaris comes up; then the cordyceps militaris is collected and dried. The method has the advantages that the temperature and the humidity are easy to control and adjust, illumination is sufficient, collection is easy, cultivation time of the cordyceps militaris is short, the operation is convenient, growth of the cordyceps militaris is tidy, high success rate of 95% or higher can be achieved and commodity of the cordyceps militaris is good.
Owner:FARM PROD PROCESSING & NUCLEAR AGRI TECH INST HUBEI ACAD OF AGRI SCI

External circulation animal cell culture bioreactor

The invention relates to an external circulation animal cell culture bioreactor which comprises a tank body, an external circulation pipeline, a heater, a peristaltic pump and a control system, wherein the tank body comprises a large tank and a small tank; the small tank is sheathed in the large tank and is clamped and fixed with the large tank respectively through ducts D, A and G; the external circulation pipeline comprises a tank external circulation pipeline and ducts A, B, E and D; on the upper part of the outside of the tank body, the ducts A and B are connected with the tank external circulation pipeline through a tee joint; on the lower part of the outside of the tank body, the ducts E and D are connected with the tank external circulation pipeline through a tee joint; the tank external circulation pipeline penetrates through the peristaltic pump; and the lowest position of the duct D is connected with a drainage pipe. The invention fully displays the characteristics of the animal cell large-scale culture technique, has the characteristics of thorough sterilization, simple operation, low pollution rate of cell culture, good quality and high yield, has favorable functionality and economical efficiency, and can be widely used for animal cell microcarrier suspension culture and animal cell suspension culture.
Owner:罗火生

Microbial culture medium preparation device and method and subpackaging method

The invention belongs to the technical field of microbial research and development, and discloses a microbial culture medium preparation device which comprises a preparation tank, a culture medium bag and a controller case, wherein the preparation tank is provided with a feed pipe and a discharge pipe; the culture medium bag is provided with a water inlet pipe and a water outlet pipe; the water inlet pipe of the culture medium bag is connected with a water source; a water inlet end of the culture medium bag is provided with a water inlet filter; the water outlet pipe of the culture medium bag is connected to the feed pipe of the preparation tank; a heating unit and a control unit are arranged in the controller case; the heating unit is used for heating the preparation tank; a temperature sensor is arranged in the preparation tank and connected to the control unit; the control unit is used for adjusting the temperature of the culture medium solution in the preparation tank in real time according to the feedback of the temperature sensor; and a stirrer is arranged in the preparation tank. By using the newly designed preparation device and preparation method, the culture medium can implement less nutrient destroy and less manual intervention, and can quickly implement preparation of the larger-volume culture medium.
Owner:上海曼森生物科技有限公司

Test method for studying high-temperature stress on wheat

The invention relates to a test method for studying high-temperature stress on wheat, and belongs to the field of plant seedling high-temperature stress tests. The test method comprises the following steps: seedling culture, variety selection, sodium hydrosulfide solution preparation, sodium hydrosulfide solution spraying, sodium hydrosulfide solution concentration optimization, sodium hydrosulfide solution spraying time and frequency optimization and the like, wherein seedling culture comprises seed selection, soaking of seeds in clean water, seed soaking and sterilization, seed soaking at a constant temperature, germination acceleration, secondary seed selection, seed arrangement, seedling acceleration, seed picking and seedling. Seedling selection and optimization is performed multiple times in the period from seed soaking to seedling transplanting during seedling, therefore, consistency of initial test materials is guaranteed. The sodium hydrosulfide solutions of different concentration are sprayed to wheat seedlings cultivated for 20 days for high-temperature stress relieving tests, and concentration, spraying time, spraying frequencies of the sodium hydrosulfide solutions are optimized; besides, in combination with optimization and innovation of a sampling method during material drawing in the test, the accuracy of final test data can be better guaranteed from test design, procedures and results, therefore, great benefit is provided for final disclosure of physiological and biochemical changes after test treatment.
Owner:SHANDONG AGRICULTURAL UNIVERSITY

Liquid culture method of wheat seedlings

ActiveCN105494062AEliminate growth inconsistenciesEliminate too many deformed seedlingsSeed and root treatmentCultivating equipmentsTest materialSolid substrate
The invention discloses a liquid culture method of wheat seedlings and belongs to the technical field of plant seedling culture. The method comprises the steps that seed selection, clean water soaking, seed soaking sterilization, constant temperature seed soaking, accelerating germination, secondary seed selection, seed arrangement, seedling hastening, seedling picking and seedling growing. In the method, in the process from seed soaking to seedling transplanting, multiple times of seed and seedling optimizing and filtering are performed, solid substrate cultivation is changed into liquid cultivation, the problems of missing seedlings and more abnormal seedlings occurring during solid substrate cultivation can be avoided, the growing uniformity of test materials can be guaranteed during seedling transplanting, and the principle that the single variant is controlled in the experiment design can be well matched. After seedling hastening, enough constantly grown seedlings with high purity can be obtained by one time and used for follow-up test, and the difficulties that the test materials grow not uniform, the malformed seedlings are too many and the seedling growth is weak caused due to the fact that wheat seeds are sowed directly can be eliminated.
Owner:SHANDONG AGRICULTURAL UNIVERSITY

A cress and traditional Chinese medicine fish cake and a preparation method thereof

The present invention discloses a cress and traditional Chinese medicine fish cake and a preparation method thereof. The fish cake is made from the following raw materials in parts by weight: freshwater surimi 25-45 parts, starch 7-10 parts, Chinese yam powder 15-25 parts, Chinese chestnut powder 2-8 parts, fresh bean dregs 1-5 parts, chicken 20-35 parts, fresh milk 4-8 parts, drinking water 5-15 parts, minced garlic 1-5 parts, minced green onion 1-3 parts, table vinegar 1-2.5 parts, spinach 7-14 parts, broccoli 3-7 parts, carrots 15-25 parts, cress 18-30 parts, table salt 1-5 parts, grape seeds 1-5 parts, nutmegs 2-3 parts, hawthorn seeds 0.5 -2 parts, fructus amomi 1-1.8 parts, mulberry leaves 2-4 parts, semen zizyphi spinosae 1-3 parts, poria cocos 1-3 parts, monosodium glutamate 1-5 parts, honey 1-5 parts, citric acid 0-0.5 parts and disodium 5'-guanylate 0-0.3 parts. The fish cake uses the surimi, traditional Chinese medicines and vegetables as main raw materials to process fish cake products to make the three materials complement each other, so that the fish cake is balanced in nutrition and rich in taste, and has a better life nourishing effect; a thorough sterilization is realized by microwave sterilization technology; the fish cake processed by the preparation method has a unique faint scent of the cress, and is green and health in raw material, unique in taste, convenient to carry, low in cost and simple in process. The fish cake has a good practical value and application prospect.
Owner:ANHUI YOUYUAN FOOD

Vacuum leak-detection sterile dual-valve soft bag transfusion combined bag

The invention relates to a vacuum leak-detection sterile dual-valve soft bag transfusion combined bag. A manufacturing process of the vacuum leak-detection sterile dual-valve soft bag transfusion combined bag comprises the following steps of sterilizing a material membrane of a liquid medicine bag, feeding the material membrane into a packing workshop, welding the material membrane with a transfusion valve to form an inner packaging bag, filling the inner packaging bag with liquid medicine to form soft bag transfusion, performing sterilization for a second time and performing drying; performing lamp detection for a first time to remove unqualified products with wrong package printing, wrong welding and large impurities in the packaging bag; covering the liquid medicine bag with an outer packaging bag, vacuumizing space between the outer protective bag and the liquid medicine bag or filling space between the outer packaging bag and the liquid medicine bag with protective gas preparations or liquid preparations or solid preparations, performing sterilization for a third time, packing the combined bag in a case, and putting the case in storage for later use; performing lamp detection for a second time to remove leaked products. By means of the vacuum leak-detection sterile dual-valve soft bag transfusion combined bag, bacteria cannot retain in an interlayer, microleaked large volume injection formed in the inner packaging bag can be discharged effectively and conveniently, and the liquid leakage rate when the combined bag leaves the factory is reduced below 0.029%. The period of validity of the vacuum leak-detection sterile dual-valve soft bag transfusion combined bag is prolonged to five years from two years.
Owner:SICHUAN TAIPINGYANG PHARMA

Light wave sterilization box, sterilizing cabinet, oven and control method thereof

The invention discloses a light wave sterilization box, a sterilizing cabinet, an oven and a control method thereof, and relates to the technical field of sterilizing equipment. Problems that the existing sterilizing cabinet is low in heating speed, long in sterilizing time and poor in sterilizing effect and a sterilizing effect cannot be achieved are solved. The light wave sterilization box comprises a box body, and annular light reflecting and focusing devices capable of generating light waves and thermal waves simultaneously are arranged on at least three different inner surfaces in the box body. Moreover, the invention provides a control method of the light wave sterilization box and a sterilizing cabinet with the light wave sterilization box. By the light wave sterilization box, comprehensive and multi-directional reflection focusing can be formed, a radiant scope is wide, dead corners do not exist during sterilization, the heating speed is high, and a sterilizing effect is achieved. In addition, because the light reflecting and focusing devices can simultaneously generate high light and thermal waves, a quick sterilizing effect is achieved through light and thermal combination, light wave sterilization is quick and thorough, and the service life is long.
Owner:QINGDAO HAIER WISDOM KITCHEN APPLIANCE CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products