Solid state fermentation reactor and application thereof

A technology of solid-state fermentation and reactor, which is applied in the direction of solid-phase fermentation bioreactor, bioreactor/fermenter combination, specific-purpose bioreactor/fermenter, etc. problem, to achieve the effect of powerful function, high heat dissipation efficiency and simple operation

Inactive Publication Date: 2015-08-19
NANJING UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The technical problem to be solved by the present invention is to overcome the problems of single function, complex structure and impracticality in the prior art, and to provide a method that can sterilize, cool, inoculate, ferment, control temperature, and control the fermentation of solid materials. Solid-state fermentation reactor with wet, ventilated and dry functions

Method used

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  • Solid state fermentation reactor and application thereof
  • Solid state fermentation reactor and application thereof
  • Solid state fermentation reactor and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] as attached figure 1 The solid-state fermentation reactor shown includes a fermenter 1 , a coil, a shallow plate and a two-fluid nozzle system 6 .

[0041] The fermenter 1 is a horizontal stainless steel fermenter, fixed on the base 3 by the first support 3-1 and the second support 3-2 at the bottom. A jacket 4-1 is provided on the periphery of the fermenter, and a steam inlet 4-2 and a steam outlet 4-3 are arranged on the jacket 4-1. The steam inlet 4-2 is arranged at the top of the jacket 4-1, and the steam outlet 4- 3 is arranged at the bottom end of the jacket 4-1, and the position of the steam inlet 4-2 and the steam outlet 4-3 in the horizontal direction is far away.

[0042] A first movable top cover 2-1 and a second movable top cover 2-2 are provided on both sides of the fermenter 1 in the horizontal direction; fixed bolts 2-3 are provided at the connection between the first movable top cover 2-1 and the side wall of the fermenter tank 1 , The connection betwe...

Embodiment 2

[0046] Embodiment 2: Aerobic microorganisms (using Bacillus subtilis solid-state fermentation to produce gamma-polyglutamic acid agricultural fertilizer as an example)

[0047] γ-polyglutamic acid (γ-PGA) is a kind of homopolyamino acid synthesized by microorganisms. The basic skeleton is formed by linking L-glutamic acid and D-glutamic acid through γ-amide bonds. straight-chain molecules, with a relative molecular mass of up to 200×10 4 Da. Due to the existence of a large number of hydrogen bonds and hydrophilic free carboxyl groups in its structure, it has excellent moisture retention properties and has been widely used in agricultural fertilizers.

[0048] The microorganism fermented in the present embodiment is the Bacillus subtilis NX-2 ((bacteria strain preservation number: CGMCC 0833) that this laboratory has obtained authorization, directly uses microbial bacterial agent (5 * 10 10 cfu / g) inoculation, the inoculation amount is 25g / kg.

[0049] Solid-state fermentati...

Embodiment 3

[0053] Embodiment 3: aerobic microorganism (taking the solid-state fermentation Bacillus subtilis phosphate-solubilizing bacterial agent as an example)

[0054] Phosphorus is one of the essential nutrients for plant growth and development. Soil phosphorus deficiency directly affects the growth of crops and food production. In my country, the area of ​​phosphorus-deficient cultivated land reaches 74%, and more than 95% of the phosphorus in the soil is in an invalid state, which is difficult to be absorbed. Plants directly absorb and utilize. There are many phosphorus-solubilizing microorganisms in the soil, which can degrade these insoluble phosphates into free forms for plant absorption and utilization. Therefore, it is necessary to develop phosphorus-solubilizing microorganisms, reduce the production cost of phosphate fertilizers, solve the pollution of phosphate fertilizers and improve the utilization rate of phosphorus. Research is necessary.

[0055] The phosphate-solubili...

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Abstract

The invention discloses a solid state fermentation reactor and an application thereof. The reactor comprises a horizontal cylindrical stainless steel fermentation tank body, a stainless steel jacket is welded on the outer wall of the above tank, and the tank is also provided with a gas outlet, probes, a steam inlet, a steam outlet, a material outlet, movable top covers and a two-fluid spray device; and coil pipes and trays are arranged in the inner cavity of the tank body. The invention also discloses an application of the reactor in cultivation of aerobic, anaerobic and facultative anaerobic microbes. The reactor has the advantages of simple structure, convenience and practicality, can solve the problems of difficult material disinfection, easy bacterial contamination, large labor intensity and low working efficiency of traditional solid state fermentation, realizes automatic control of the solid state fermentation process, and facilitates industrial application of the solid state fermentation.

Description

technical field [0001] The invention belongs to the technical field of fermentation engineering and process engineering, and relates to a solid-state fermentation reactor and its application. Background technique [0002] Solid-state fermentation refers to the biological reaction process of one or more microorganisms on a solid substrate in the presence of no or almost no free water. It has the advantages of simple operation, low energy consumption, and easy control of the fermentation process. Agricultural residues and industrial wastes are used as solid substrates. At present, in the biomanufacturing industry, submerged fermentation is still in a dominant position. The reason is that there is a lack of a multi-functional, stable fermentation reaction device. Existing solid fermentation devices mainly include shallow plate fermentation, aerated fermentation tank, drum type fermentation, etc., but shallow plate fermentation conditions are generally rough, poor ventilation a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/34C12M1/16C12M1/12C12M1/04C12M1/02C12M1/00
Inventor 冯小海汤宝梁金丰徐虹许宗奇姜永翔
Owner NANJING UNIV OF TECH
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