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A fermentation method and gibberellic acid technology, applied in the field of fermentation, can solve problems to be improved, and achieve the effects of ensuring synthesis and metabolism, fast and stable fermentation, and increasing acid production.
Active Publication Date: 2018-07-17
SICHUAN LOMON BIO TECH CO LTD
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[0004] The existing preparation of gibberellic acid usually adopts liquid submerged fermentation method. During the fermentation process, soybean oil or glucose is added in batches or batches. The higher level of fermentation acid production obtained is 2800-3000ppm, but it still needs to be improved.
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Embodiment 1
[0045] 1.1 Will GA 3 The strain freezing tube was directly connected to the shake flask, and the inoculum amount was 0.8% of the volume of the culture medium in the shake flask. The medium formula in the shake flask was shown in Table 1. The fermentation temperature was 29±0.2°C, and the shake flask was cultivated for about 68 hours to obtain a suspension.
[0046] Table 1 shake flask culture medium formula
[0047]
[0048] 1.2 Inoculate the suspension into the primary seed tank, the inoculation amount is 0.016% of the volume of the culture solution, and the medium formula of the primary seed tank is shown in Table 2.
[0049] Table 2 Primary seed tank culture medium formula
[0050]
[0051]
[0052] Fermentation volume: constant volume 1.3t, the volume after digestion is calculated as 1.5t (add sulfuric acid before sterilization to adjust the pH to 5.1).
[0053] Fermentation temperature: 29±0.2°C.
[0054] Operating tank pressure: 0.035MPa.
[0055] Air flow: ...
Embodiment 2
[0084] The medium formulation and culture conditions are the same as in Example 1.
[0085] Feeding control: Dissolved oxygen rebounds by 20%, automatic feeding of glucose (45%) solution is started, and the dissolved oxygen control point is 20% to 30%.
[0086] The fermentation broth obtained in Example 2 was detected, and the results obtained are shown in Table 8 and Table 9.
[0087] Table 8 Fermentation broth detection data
[0088]
[0089]
[0090] Table 9 Wet weight of fermentation broth
[0091]
Embodiment 3
[0093] The medium formulation and culture conditions are the same as in Example 1.
[0094] Feeding control: Dissolved oxygen rebounds by 20%, automatic flow of salad oil is turned on, and the dissolved oxygen control point is 20% to 30%.
[0095] The fermentation broth obtained in Example 3 was detected, and the results obtained are shown in Table 10 and Table 11.
[0096] Table 10 fermentation broth detection data
[0097]
[0098] Table 11 Wet weight of fermentation broth
[0099]
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Abstract
The invention provides a fermentation method of gibberellic acid. The method comprises the steps that a seed solution of gibberellic acid is transferred into a fermentation tank for fermentation to obtain a gibberellic acid fermentation liquid; when the dissolved oxygen rate is higher than 20% in the fermentation process, glucose and vegetable oil are added. Compared with the prior art, glucose and vegetable oil are adopted for blending in the fermentation process, and the acid yield is increased; meanwhile, corn protein powder serves as an organic nitrogen source, the protein content of the corn protein powder is high and reaches 50% or above, and high-density fermentation of gibberellin can be achieved; the hidden danger that aspergillus flavus is generated from peanut cake powder can beavoided, synthesis and metabolism of thalli are ensured, and the thalli can be rapidly and stably fermented; furthermore, the vegetable oil is added to provide a carbon source for fermentation, the phenomena of fermentation bubble escaping and the like can be effectively reduced, and the potential risks such as material loss and bacterial contamination are avoided; in addition, trace elements areadded into a fermentation culture medium so that the activity of various enzymes and metabolites in the microbial growth metabolism process can be comprehensively ensured.
Description
technical field [0001] The invention belongs to the technical field of fermentation, and in particular relates to a fermentation method of gibberellic acid. Background technique [0002] Gibberellic acid is a type of secondary metabolite obtained through the fermentation and cultivation of Gibberella. There are 116 species that have been isolated, identified and named, of which GA is the most widely used in agriculture. 3 , plays a huge role in my country's agricultural production. [0003] GA 3 It has very high plant regulation activity, and has obvious regulation effects on the growth and development of various crops. in North, GA 3 It is mainly used in economic crops such as grapes, red dates, hawthorns, nectarines, etc. Spraying a certain concentration of gibberellic acid solution during the flowering period can effectively increase the fruit setting rate of fruit trees, promote fruit growth and development, and increase the yield of fruit trees by about 20% to 30%. ...
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