360 results about "Shake-Flask Culture" patented technology

The invention discloses a high-yield strain streptomyces lydicus, as well as breeding and fermentation thereof. The fermentation of the high-wield stain streptomyces lydicus E9 CGMCC No.3075 comprises the following steps: (1) preparation of a shake flask, seeds and a fermentation medium; (2) shake flask culture; (2) seed culture; (4) fermentation pot culture: leading a seed culture liquid in a fermentation pot filled with the fermentation medium and culturing for 24-96 hours to obtain a high-wield stain streptomyces lydicus E9 fermentation liquor under the condition of ventilating, stirring, temperature of 25-35 DEG C, and pH of 4-9; and carrying out ultrasonication for 20-40 min. The strain of the invention can generate large quantity of substances with antimycotic activity in the fermentation liquor, and the fermentation liquor has evident inhibiting effect on rhizoctonia solani; and the bacterial inhibition rate of the fermentation liquor which is diluted by 50 times reaches above 97.8% after 48 hour fermentation.

The present invention relates to a lipasegenic bacterium, its screening method and its industrial application. Said strain is a rhizopchin obtained by separating Chinese daqu liquor, its name is Rhizopus chinensis CCTCC M201021. Its screening method includes the following steps: taking daqu liquor sample or unstrained spirits, diluting and coating on plate, slant culture, shake-flask culture, centrifugal separation, injecting supernatant fluid into preliminary screen plate hole, selecting bacterium with larger transparent ring and / or fluorescent ring to make shake-flask fermentation and rescreening, freeze-drying thallus so as to obtian whole cell rhizopchin lipase enzyme preparation. It can be used for conversion synthesis of aromatic ester in orgnaic phase.

The invention relates to the biological treatment for oil extraction wastewater, which in detail relates to a bacterial agent for treating thick oil wastewater and the method for preparing the same. The comprised components and their proportion by weight are as follows: pseudomonas aeruginosa 5-10%, bacillus subtilis 10-15%, lichen bacilli 5- 15%, Citrobacter propionate tumefaciens 10- 15%, liquid gold tumefaciens 10- 20%, annular gemma bacillus 5- 10%, wilting Bacillus pumilus 5- 10%, spherical arthrobacter 5- 10%, crabstick tumefaciens 5- 10%, and hot ground anaerobic rod baceria 10- 15%. It is prepared by activating bacteria, shake-flask culturing, expanding propagating and mixing. The cooperation action is strong, the biological surface activating agent generating- bacteria can enlarge the dissolution of hydrocarbon petroleum in water and make it is easier for hydrocarbon petroleum to contact with bacteria; the anaerobic bacteria can improve the biodegradation of thick oil wastewater; the degradation perfomace of aerobic bacteria is good and suitable for biological treatment for thick oil wastewater.

The solid fermentation process for producing natto bacillus feed additive with bean dregs includes the following steps: 1. shake flask culture of natto bacillus in KMB culture medium to obtain the first stage spawn; 2. dissolving bean dreg powder, corn starch, corn slurry and NaCl in water, regulating pH value, sterilizing and inoculating the first stage spawn, and shake flask culture to obtain the second stage spawn; 3. mixing water soaked bean dregs, NaCl, glucose as the carbon source, gelatin as the nitrogen source and (NH4)2SO4 or urea, and sterilizing to obtain solid culture medium, inoculating the second stage spawn, fermenting while maintaining the temperature and humidity to obtain fermented bean dregs; and 4. adding sterilized corn powder into the fermented bean dregs, drying and crushing to obtain the natto bacillus feed additive. The feed additive can promote the growth of pig, promote the growth of lactobacillus and inhibit colibacillus.

The invention relates to a preparation method of rhamnolipid biological fermentation liquid. The method comprises the following steps: extracting and purifying Pseudomonas aeruginosa obtained from petroleum-contaminated soil, and obtaining Pseudomonas aeruginosa VTS-1 (Pseudomonas sp. -1) The preservation number is: CGMCC2200, and the specific fermentation and cultivation steps are as follows: A. Strain VTS-1 is cultured in shake flasks; B. Primary fermentation cultivation; C. Secondary fermentation cultivation; D. Fermentation cultivation. The process of the method has the advantages of high yield, high efficiency, high loading coefficient, short fermentation cycle, simple process, reduced overall product cost, and is completely suitable for industrial production, and has played a role in the promotion and large-scale application of rhamnolipid biosurfactants. important role.

The invention provides a preparation method of ganoderma lucidum mycelium slices, which can be used for solving the technical problem that ganoderma lucidum mycelium powder is inconvenient to eat in the prior art. The preparation method comprises the steps of preparation of a culture medium, activation of strains, inoculation and culture, baking, puffing, crushing, mixing, granulating and tabletting, wherein the preparation of the culture medium comprises the steps of: A, preparation of a grain culture medium; B, preparation of a slant culture medium; C, preparation of a shake flask culture medium; D, preparation of a fermentation tank culture medium. The preparation method can be widely used in the processing of the ganoderma lucidum mycelium slices.

A process for preparing the aqueous extract and polyose from the Sanghuang fungus in large scale by deep liquid fermenting includes slo slant shake-flask culture, enlarge culture, class-one seeding culture, fermenting culture, heating while stirring for 2-4 hr, press fitlering, concentrating, spray drying to obtain its aqueous extract or depositing in alcohol, dissolving deposit in water, and spray drying to obtain its polyose.

The invention discloses a non-invasive biomass online detection device used for shake-flask culture. The device comprises a master control unit, a shake flask capable of containing biological turbid liquid and a fixing sleeve covering the outer side of the shake flask. A detection probe is connected to the fixing sleeve on the outer side of the bottom of the shake flask. The detection probe comprises a substrate capable of being attached to the bottom of the shake flask, a light source, a photoelectric sensor and an acceleration sensor, wherein the light source, the photoelectric sensor and the acceleration sensor are arranged on the substrate. The light source can generate a light path obliquely irradiated into the shake flask. The photoelectric sensor is located below the light path and used for measuring scattering light generated after the light path is irradiated to the biological turbid liquid. The light source, the photoelectric sensor and the acceleration sensor are all connected with the master control unit. According to the non-invasive biomass online detection device, a turbidity measurement method is adopted for performing online detection on the concentration of bacterial liquid cultured in the shake flask through a back scattering light measurement technology, and the concentration of the biological turbid liquid in the shake flask can be detected under the condition that culture equipment is not broken. The non-invasive biomass online detection device is simple in structure, low in cost, and capable of effectively improving detection efficiency and precision and avoiding sample contamination.

The invention discloses a preparing method of nano-soy capsule, which comprises the following steps: removing stone and impurities in the soy, cleaning, soaking for 15-20 h, boiling under 0.13Mpa for 30-40 min, seeding bacillus subtilis according to 2% in the raw material weight under 80-90 deg.c aseptic condition, fermenting at 37-40 deg.c for 20-24 h, culturing seed through shake flask, freezing fresh nano-soy at -35- -40 deg.c, transmitting into cryodesiccation chamber, screening, grinding, affirming rate to blend, filling to obtain the product.

A streptomyce shygeroscopicus (CCTCC No.M203062) able to generate glutamine transaminase wigh high output is extracted from the soil of milk cow farm through plate screening, primary fermenting, flask culture, comparing test with standard color tape, and enzyme activity test. Said glutamine transaminase is prepared by deep fermentation.

The invention relates to a complex microbial inoculum for processing human and animal excreta efficiently, which consists of bacillus subtilis, bacillus licheniformis, geobacillus stearothermophilus, streptococcus thermophilus, geotrichum candidum, aspergillus niger and heat amylase streptomyces. A preparation method comprises the following steps of first-stage shake flask culture, second-stage shake flask culture, and third-stage solid medium culture of the complex microbial inoculum for the strains. The preparation method is simple with low use cost, saves resources, can be widely applied to stationary type or wheeled lavatories, and accords with the policy of sustainable development.

The invention relates to a preparation method of DHA (which relates to an unsaturated fatty acid. The invention provides a preparation method of a fully synthetic medium that is suitable for high density growth of fissiparism chytrid and increases the output of the DHA. The DHA comprises a carbon source, amino acids, lysine, inorganic salts, vitamins, trace elements and water. The carbon source is glucose, the amino acids comprise alanine, methionine, cysteine, lysine, histidine, glutamic acid, glutamine, isoleucine, threonine and tryptophan; the vitamins comprise VB1, VB12, biotin, lipoic acid and folic acid; the inorganic salts comprise NaCl, KCl, MgCl2, CaCl2, Na2HPO4 and KH2PO4; and the trace elements comprise AlCl3, H3BO3, ZnSO4, MnCl2, CuSO4, FeSO4 and (NH4)6Mo7O24.4H2O. The synthetic medium is utilized for culturing the fissiparism chytrid based on a shake flask, and the output of the DHA is 9.8g / L.

An alpha-ketoglutaric acid high-yield strain and a method for sieving and preparing alpha-ketoglutaric acid through strain by fermenting belong to the chemical engineering technique field. The strain of the invention is Yarrowia lipolytica WSH-Z06 and the preservation number is CCTCC NO: M207143. The strain is aneurine nutriment defect strain which is contrasted and sieved from the soil near the oil refinery according to the colony growth condition on the complete culture medium, basic culture medium and complementary culture medium flats, and then the aneurine nutriment defect strain is adopted by one-grade ferment to do shake flask culture one by one and sieve primarily. The fermentation liquor is analyzed by silica gel chromatography. The strain which keeps alpha-ketoglutaric acid colour development spot is transferred in the shake flask which is loaded with ferment culture medium to do ferment sieve again. The fermentation liquor is tested by high-effective liquid-phase method and produces the high-yield alpha-ketoglutaric acid strain compared with the production of alpha-ketoglutaric acid. The production of alpha-ketoglutaric acid can reach 39.3 g / L through optimizing the important component in the ferment culture medium and fermenting for 6 d.

The invention discloses an Agaricus Bisporus liquid spawn and a preparation method thereof. The Agaricus Bisporus liquid spawn is produced by means of three-level propagation through slant and shake-flask media and a fermenter medium. The slant and shake-flask media comprise components including 100-200g of potato, 50-150g of cow dung, 10-30g of glucose, 1-2.5g of mono potassium phosphate, 0.5-2g of magnesium sulfate and 1-4g of peptone; the fermenter medium of every 50 liters of water is composed of 2-4kg of potato, 1-3kg of cow dung, 0.5-1kg of bran, 0.2-0.4 kg of corn powder, 0.5-1kg of sucrose, 30-60g of mono potassium phosphate, 20-30g of magnesium sulfate, 120-150 mg of vitamin B and 40-50g of defoamer. By application of the Agaricus Bisporus liquid spawn and the preparation method thereof, the problem that mycelia are prone to aging and degradation during growth is solved; the liquid spawn is of globular mycelia with uniform age, high activity and fast running; a high-running spawn center is formed after the liquid spawn is inoculated to solid media, with uniform fruiting achieved; the preparation cost is only one-tenth of that of solid spawn; the growth cycle of fermenter spawn is 7-9 days, and thus spawns on a large scale can be cultured within a short time.

The invention provides a method of producing Agrocybe aegerita strains by liquid culture and a preservation method of the Agrocybe aegerita strains. The method includes the steps and process parameters: liquid culture efficient strains are screened, namely the liquid culture strains with many pellets, uniform size and many surface burrs are optimally selected, the amount of culture pellets is 200-260 / ml, the pellets are 1.0-1.5mm in diameter, a shake-flask culture formula includes 1-3% of glucose, 2-4% of corn flour, 0.2-0.4% of yeast powder, 1.0-3.0% of wheat bran, 0.5-1.5% of KH2PO4 and 0.5-1.0% of MgSO4, with natural PH value, the culture medium formula for fermenting tank culture of Agrocybe aegerita liquid strains is provided, and process conditions for fermenting tank culture of Agrocybe aegerita liquid strains are established. The key technical problems of production of Agrocybe aegerita strains are solved by the production of Agrocybe aegerita strains by liquid culture; the production cycle is short, production cost is low, fruiting is even after inoculation, and management is facilitated.

The invention provides a method for postprocessing of hericium erinaceus fermentation mycelium solution and preparing mycelium powder through biological enzyme. The method solves the problems that according to existing hericium erinaceus mycelium, the fermentation period is long, contamination is prone to appearing, and the final product quality is not stable and also solves the problems that the number of mycelium fibers in an existing hericium erinaceus fermentation mycelium solution is large, the fibers cannot be easily separated, and further preparation of the mycelium powder is limited. The method comprises the steps of preparing a culture medium, activating a culture, inoculating, performing cultivating, expanding fermentation, performing enzymolysis, performing concentrating, performing homogenizing, performing spraying and performing drying. The method for preparing the culture medium comprises the steps of preparing an inclined plane culture medium, preparing a shake flask culture medium, preparing a seeding tank culture medium, and preparing a fermentation tank culture medium. The method is widely applied to processing the hericium erinaceus mycelium powder in the food industry.

The invention belongs to the technical field of biology, and particularly relates to screening of a strong-stability moderate-temperature neutral amylase high-producing Bacillus subtilis XLG-1 and preliminary study on the zymologic property of the enzyme. The strain is preserved at China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC NO.3871. The biological characteristics of the strain are as follows: the strain is in shape of a long pole; and on a starch-screening solid culture medium, the bacterial colony is shaped like a circle with uneven edges, and the lawn is dry and nontransparent and is milk white in color. Under the condition of shake culture for 72 hours at 37 DEG C, the enzyme activity can be up to 2200U / mL; and after ultraviolet-lithium chloride compound induction mutation, the maximum enzyme yield can be up to 6183U / mL. The enzyme can widely used in multiple fields, such as detergent, beer, food, medicine, leather manufacturing, weaving, paper making and other industries.

The invention relates to a factorization strain production method for hypsizygus marmoreus and a cultivation method for the hypsizygus marmoreus. The factorization strain production method for the hypsizygus marmoreus comprises inserting inclined plane stock culture into a flat plate to cultivate for 12-14 days at the temperature of 22 DEGC to 25 DEG C; then transferring flat plate strains into a shake flask filled with shake flask culture media to cultivate for 6-8 days at the temperature of 22 DEGC to 25 DEG C to obtain shake flask strains; then transferring the shake flask into a fermentation tank filled fermentation media, keeping temperature at 22 DEGC to 25 DEG C, maintaining ventilation pressure at 0.05-0.15MPa, and cultivating for 6-8 days to obtain fermentation tank liquid strains; inserting the fermentation tank liquid strains into a stock bottle filled with stock materials to cultivate for 35-40 days in dark to achieve stock, and picking and sterilizing to obtain factorization strains of the hypsizygus marmoreus. The factorization strain production method for the hypsizygus marmoreus has the advantages of being short in period and high in quality, can guarantee advantages of solid strains on mycelium stimulation mode of the hypsizygus marmoreus and hypsizygus marmoreus output quality and has good popularization value.

The invention discloses a process for biodegrading yellow water of a white spirit factory, and relates to the technical field of bioengineering. According to the process, aspergillus oryzae is utilized as a starting strain, and the COD (Chemical Oxygen Demand) of the yellow water of the spirit factory is reduced through a biological fermentation process. The process comprises the flow consisting of the following steps of: inoculating the aspergillus oryzae; performing shake-flask culture and first-stage seed culture; inoculating into diluted yellow water of the white spirit factory; and fermenting and culturing. By using the method, the COD removal rate can reach 60-98 percent, the BOD (Biochemical Oxygen Demand) removal rate reaches 90-99 percent, the pH is increased from original 3.5 to 7.0-8.0, the produced wastewater is subjected to centrifugation and molecular interception, and thalli and active molecules serving as feed additives can be obtained.

The invention provides a processing method of compound hypha powder, solving the technical problem of high possibility of nutrition loss caused by an existing lucid ganoderma culturing method. The processing method comprises the steps of: preparing a culture medium, activating a strain, inoculating for culturing, drying, puffing, crushing, sterilizing and bagging. The step of preparing the culture medium comprises the sub-steps of: A, preparing a grain culture medium; B, preparing an agar slant culture medium; C, preparing a shake flask culture medium; and D, preparing a fermentation tank culture medium. The processing method can be widely applied to processing of compound hypha powder.

The invention discloses a bacillus licheniformis and a method for utilizing the sludge as raw material for producing alkaline protease. The bacillus licheniformis is gram-positive bacteria and is shaped like a straight rod, and the collection number is CGMCC 1970. The method for utilizing the bacillus licheniformis for producing the alkaline protease includes (1) the conditioning of a culture medium; (2) shaking culture; (3) the fermentation by a fermentation tank; and (4) the purification of the protease. The bacterial strain of the invention is utilized for producing the alkaline protease, which can not only dispose sludge, but also obtain the alkaline protease product with high additional value and lower cost. The method uses urban waste sludge to replace the traditional food culture medium for producing the alkaline protease product, which not only provides a new way for the resource disposal of urban sludge, but also can greatly reduce the price of the product and promote the industrial production and application of protease preparations.

The invention discloses a preparation method for tetracycline. The preparation method comprises the following specific steps of preparing a streptomyces aureus strain and then performing seed shake-flask culture and seed tank culture; when culture is performed for 20-26 hours, converting the culture mode into fermentation culture; when the pH value reaches 5.6-7.6, the total titer is not smaller than 8,000U / ml, the total saccharides content is not greater than 2.0 percent, the quantity of amino nitrogen is greater than 50mg / 100mL, and the filtering velocity is not smaller than 5mL / 5min, ending the fermentation and discharging from a tank; finally, filtering with a plate frame, decoloring ion exchange resin, crystallizing and performing air current drying to obtain the tetracycline. According to the preparation method disclosed by the invention, a fermentation process is simple, the fermentation unit can be effectively improved, the yield is improved, and the production cost is reduced; meanwhile, the use of chemical organic solvent is avoided.

The invention discloses a method for producing functional foods by simultaneously converting Chinese yam from cordyceps taishanensis, and relates to the technical field of bioengineering technology. The preparation method comprises the steps of test tube enlarged cultivation, liquid shake-flask culture and seeding tank enlarged cultivation, solid fermentation culture, drying and smashing in sequence to obtain the functional foods with the effects of regulating blood lipids, blood sugar and blood pressure, resisting radiation and inhibiting tumors. Chinese yam, rice, wheat, corn, sorghum and the like are adopted as a solid substrate, cordyceps taishanensis is adopted as culture, Chinese yam saponin is converted through solid fermentation, and cordycepin and cordyceps polysaccharide are synthesized at the same time; according to the obtained product, the content of saponin of the dry substrate is 2-20 mg / g, the content of sugar of the dry substrate is 10-100 mg / g, and the content of cordycepin of the dry substrate is 1-20 mg / g. The foods can be used for extracting saponin, cordyceps polysaccharide and cordycepin, and producing tablets or capsules for treating and lowering high blood fat, high blood sugar and high blood pressure, resisting radiation and inhibiting tumors.

The invention discloses high-efficiency phosphate-solubilizing aspergillus japonicus with heavy metal tolerance. The high-efficiency phosphate-solubilizing aspergillus japonicus with the heavy metal tolerance is classified and named as aspergillus japonicus, and is collected in China General Microbiological Culture Collection Center; the collection number is CGMCC No.7700. The invention also discloses the tolerance of the high-efficiency phosphate-solubilizing aspergillus japonicus to heavy metals. The high-efficiency phosphate-solubilizing aspergillus japonicus has an extremely good dissolving effect on insoluble phosphates, such as tricalcium phosphate, magnesium phosphate and aluminum phosphate in the situation of liquid shake flask culture; the dissolving effects on the three types of insoluble inorganic phosphorus reach 100 percent. The aspergillus japonicus has certain tolerance to the heavy metals after being incubated in liquid culture solutions which contain the heavy metals such as Pb<2+>, Zn<2+>, Cr<2+>, Mn<2+>, Cu<2+>, As<5+> and Cd<2+>; particularly, the tolerated concentrations of the Pb<2+>, the Zn<2+>, the Cr<2+> and the Mn<2+> reach 2,000 mg / L respectively. Therefore, the high-efficiency phosphate-solubilizing aspergillus japonicus with heavy metal tolerance can provide excellent strain resources for the development of heavy metal-resistant high-efficiency phosphate-solubilizing bacterium agents.

The invention discloses a culture method of halimasch liquid bacterial strains. The culture method comprises the steps of parent strain preparation, shake-flask culture and fermentation tank culture. The culture method has the advantages that the raw material cost is low, the obtaining is easy, in addition, seed bacteria are subjected to fragmentation treatment, bacterial balls can become small and have the same size, the quality of the seed bacteria is improved, the growth speed of the bacterial balls is accelerated, the fermentation growth of the halimasch deep-layer liquid is fast, and the yield is greatly improved. The still standing suspension culture is adopted in the culture method, bacterial strains absorb nutrition from the culture media and absorb oxygen from air, and mycelia downwards grow until thick and strong rhizomorphs are grown and are fully filled into the whole fermentation tank. Halimasch of the obtained rhizomorphs can reach 35 to 40g through being metered by a dry weight method. Compared with the solid fermentation or the ordinary liquid deep-layer fermentation, the culture method has the advantages that the operation is simple, the separation is convenient, the period is short, the rhizomorph yield is high, and the active polysaccharide yield is also high and stable.

The invention provides a method for preparing high-purity lucid ganoderma hyphostroma powder through liquid submerged fermentation. The method solves the problems that the existing solid fermentation period of lucid ganoderma hyphostroma is long, the lucid ganoderma hyphostroma is prone to being contaminated by bacteria, the quality of the final product is unstable, and the content of active ingredients is low. The method includes the steps of culture medium preparation, strain activation, inoculation and culture, fermentation expansion, concentration, homogenization, spray drying and the like. The culture medium preparation step includes the procedures of A, agar slant culture medium preparation, B, shake flask culture medium preparation, C, seeding tank culture medium preparation and D, fermentation tank culture medium preparation. The method is widely used for processing lucid ganoderma hyphostroma powder in the food industry.

The invention discloses a preparation method and product of functional red pitaya yeast. The preparation method comprises the steps of preparing a pitaya culture medium; preparing a red yeast mycelium: culturing Monascus purpureus yang TY622 serving a strain in a slant culture medium and a liquid shake flask culture medium, meanwhile, carrying out compound mutation on the strain by using ultraviolet ray and diethyl sulfate, then, connecting the strain to a fermentation medium, and carrying out liquid fermentation culture to obtain the red yeast mycelium; and preparing the functional red pitaya yeast: connecting the red yeast mycelium, probiotics or lactic acid bacteria to a pitaya pulp culture medium, and carrying out liquid fermentation to obtain the functional red pitaya yeast. The functional red pitaya yeast disclosed by the invention simultaneously has various health-care functions of pitaya and red yeast, and not only has the red yeast effects of reducing blood fat, reducing blood pressure, reducing blood sugar and the like, but also has the pitaya effects of expelling toxins, beautifying the face, preventing senile lesion, eliminating heavy metal poisoning and the like, so that demands of more people can be met.

The invention discloses a plant-growth-promoting endophytic bacterium having a polycyclic aromatic hydrocarbons degrading function and application thereof. The plant-growth-promoting endophytic bacterium having the polycyclic aromatic hydrocarbons degrading function, namely Massilia bacterium Pn2 is preserved in China General Microbiological Culture Collection Center and has the preservation number of CGMCC 1.12927. By virtue of a microbial agent prepared by the strain, the degradation rate of phenanthrene of which the initial concentration is 150mg.L<-1> can reach up to above 95% within 3 days under the condition of laboratory shake-flask culture and meanwhile, naphthalene, acenaphthene, anthracene and pyrene can be degraded in different degrees by virtue of the bacterium, the microbial agent is colonized on the surface of a plant root by virtue of a root dipping treatment and then the plant is planted in a nutrient solution contaminated by virtue of phenanthrene so that the content of phenanthrene within a plant body can be reduced by above 50% within short time, the risk of phenanthrene contamination of the plant body is obviously reduced and the endophytic bacterium has a significant plant growth promoting effect.

The invention discloses a temperature-controlled liquid culture method capable of improving the yield of ganoderma lucidum polysaccharides and relates to the technical field of fermentation engineering. The temperature-controlled liquid culture comprises two steps of liquid seed culture and shake-flask culture, each step comprises three fermentation processes, and the rotation speed, the culture temperature and the culture medium components in each fermentation process are regulated and controlled to change the growth and development states of ganoderma lucidum mycelia so as to improve the yield and the quality of ganoderma lucidum polysaccharides. Meanwhile, the temperature-controlled liquid culture method is simple and has high economic benefits.

The invention discloses a method for efficiently producing ganoderan through semicontinuous liquid culture. The method comprises the following steps: (1) carrying out activating and expanding propagation on mother strains; (2) preparing strains through shake-flask culture; (3) preparing a seed solution; (4) carrying out synergetic inducing treatment by adopting nitric oxide and ethylene; and (5) carrying out semicontinuous liquid culture. With the semicontinuous liquid culture method, the logarithmic phase of lucid ganoderma mycelia can be effectively prolonged, so that the liquid culture biomass of lucid ganoderma is improved, and the yield of ganoderan is improved. Compared with the conventional batch culture method, the method provided by the invention has the advantages that the process is reasonable, the operation is simple, the non-fermentation time used for repeated tank washing, sterilizing and the like can be saved, thus the production efficiency and the equipment utilizationrate are improved, the production cost is reduced, and the method can be used for producing ganoderan in large scale, and belongs to the liquid culture method for lucid ganoderma with a great application prospect.