43results about How to "Achieve high density fermentation" patented technology

The invention relates to a high-density enterococcus faecalis fermentation culture medium and a fermentation process thereof. The fermentation culture medium is prepared from 20-30 g / L of glycerol, 10-15 g / L of soybean meal, 1-3 g / L of ammonium sulfate, 2-3 g / L of potassium hydrogen phosphate, 3-8 g / L of sodium acetate trihydrate, 2-4 g / L of trisodium citrate dihydrate, 0.3-0.8g / L of magnesium sulfate heptahydrate and 0.1-0.3 g / L of manganese sulfate monohydrate. The fermentation process comprises the steps that high dissolved oxygen (25%) is adopted and combined with glycerin to serve as a fermentation carbon source, the pH value of the constant sodium carbonate fermentation liquid is 5.5, the glycerol concentration is controlled to 10 g / L by adding a supplementary material in the fermentation process in a flowing mode, culture is performed for 16 hours, and then the fermentation is completed when OD (600 nm) is greater than 35, wherein the viable count of the enterococcus faecalis contained in the fermentation liquid can be up to 8*1010 CFU / mL or above. In the fermentation mode that the high dissolved oxygen is combined with the glycerin to serve as the carbon source, the lactic acid concentration in the fermentation liquid is reduced, the enterococcus faecalis contained in the fermentation liquid can be up to 8*1010 CFU / mL or above, the biomass separation cost is reduced, a production cycle is shortened, the production cost is reduced, and the production efficiency is improved.

The invention discloses a method for producing keratinase, which belongs to the technical field of microorganisms and fermentation. The present invention proceeds from two aspects of fermentation medium components (carbon source, nitrogen source, metal ion, phosphate) and fermentation conditions (temperature, pH, inoculum size), and utilizes Bacillus subtilis to ferment and produce the technique of keratinase. optimization. Utilizing the method of the invention to ferment and produce keratinase can increase the yield of keratinase by 3.3 times compared with the control, and the enzyme is produced in a 3L fermenter, and the enzyme activity reaches 704400U / mL. Therefore, the method of the present invention has broad application prospects in the preparation of keratinase in feed, tanning and the like.

The invention provides a preparation method of gibberellic acid, comprising: S1) preparing gibberellic acid seed liquid; S2) transferring the gibberellic acid seed liquid into a fermenter for fermentation to obtain gibberellic acid fermentation liquid; S3 ) concentrating the gibberellic acid fermentation broth through membrane filtration to obtain a concentrated solution; S4) refining the concentrated solution to obtain gibberellic acid. Compared with the prior art, the present invention uses sugar and oil mixed supplementation in the fermentation process to increase the amount of acid production. At the same time, corn gluten powder is used as an organic nitrogen source, and its protein content is higher, reaching more than 50%. High-density fermentation can avoid the hidden dangers of Aspergillus flavus in peanut cake powder, ensure the synthesis and metabolism of bacteria, and enable rapid and stable fermentation; moreover, adding vegetable oil can not only provide carbon sources for fermentation, but also effectively Reduce the occurrence of fermentation bubbles escaping, and avoid potential risks such as material loss and bacterial contamination.