43results about How to "Achieve high density fermentation" patented technology

The invention relates to a kind of Bacillus mucilaginosus and a culture method and culture medium thereof, belonging to the microbiological technical field. The Bacillus mucilaginosus P.muc3016 has the functions of nitrogen fixation, phosphorus-dissolving and potassium-releasing and belongs to the Paenibacillus group. The batch fermentation method is adopted to ferment and culture the Bacillus mucilaginosus and the concentrations of carbon source and dissolved oxygen are monitored and regulated. The culture medium contains carbon source, soybean meal powder, ammonium sulfate, magnesium sulfate, potassium dihydrogen phosphate, dipotassium hydrogen phosphate, calcium sulfate and calcium carbonate, and the pH value of the culture medium is 7.5-7.0. The Bacillus mucilaginosus of the invention has three functions of nitrogen fixation, phosphorus-dissolving and potassium-releasing simultaneously, has higher function activity and can obviously increase the crop yield; and by adopting the culture method and culture medium of the invention, the fermentation density can be increased and the fermentation time can be shortened.

The invention discloses a fibrous bed reactor and the equipment and process for extracting separation and coupling production of propionic acid. The invention utilizes an immobilized fibrous bed reactor unit, a membrane separation unit, an extraction and anti-extraction unit and an elution and condensation unit to realize the efficient production of propionic acid. The device has strong feasibility, simple operation, easy realization of automation, immobilized materials, long using life of the membrane and the matching equipment, convenient maintenance and good application prospect. Through extracting separation and coupling production technology, the separation of propionic acid is realized. The invention radically eliminates the feedback inhibition of propionic acid, implements the high density fermentation of propionibacterium and greatly improves the production efficiency of propionic acid through the fermentation method. The extracted water phase is returned to the reactor for recycle after elution and condensation by an elution device, which eliminates the harm of extractant for propionibacterium from the root.

The invention discloses a pseudomonas mendocina. The pseudomonas mendocina is preserved in Guangdong Culture Collection Center on December 12, 2017 with a biological preservation number of GDMCC 60297. Correspondingly, the invention also discloses a culture medium of the pseudomonas mendocina. The culture medium of the pseudomonas mendocina comprises a seed medium, a fermentation medium and/or a fed-batch medium, wherein the fermentation medium is composed of 15-30 g/L of starch, 6-14 g/L of dried corn steep liquor powder, 4-8 g/L dipotassium phosphate, 2-5 g/L of ammonium sulfate and 0.1-0.4g/L of magnesium sulfate heptahydrate. Correspondingly, the invention also discloses a fermentation process of the pseudomonas mendocina and application of the pseudomonas mendocina to sewage treatment. The fermentation process of the pseudomonas mendocina can achieve low-cost and high-density fermentation of the pseudomonas mendocina.

The invention belongs to the technical field of agricultural microbes, and relates to a high-density fermentation culture medium formula for saccharomyces cerevisiae for feed and applications thereof. The culture medium is composed of starch with a concentration of 18 to 22 g/L, cane sugar with a concentration of 5 g/L, beef extract with a concentration of 10 g/L, urea with a concentration of 10 g/L, and yeast extract with a concentration of 5 g/L. The fermentation technology conditions are as follows: temperature is 25 DEG C, pH value is 6 to 6.5, and the fermentation is performed for 20 to 24 hours in a primary throughput of 1.2:1. Two to three hours after the absorption value reaches 1.4 under the waves with an OD value of 600 nm, liquid is supplemented according to a volume ratio of 1:10. The supplement liquid is composed of starch with a concentration of 100 g/L, cane sugar with a concentration of 25 g/L, beef extract with a concentration of 50 g/L, urea with a concentration of 50 g/L, and yeast extract with a concentration of 25 g/L. The fermentation density (live bacterium number) of saccharomyces cerevisiae in a provided culture medium is increased by 7.3 times compared to that of saccharomyces cerevisiae in a YPED culture medium. The bacterium concentration reaches 1.05*109 cfu/mL, and the dry bacterium weight reaches 112 g/L. The using amount of peptone and glucose is reduced, thus the production cost is reduced, and the culture medium is more suitable for industrial production.

The invention provides a fermentation method of gibberellic acid. The method comprises the steps that a seed solution of gibberellic acid is transferred into a fermentation tank for fermentation to obtain a gibberellic acid fermentation liquid; when the dissolved oxygen rate is higher than 20% in the fermentation process, glucose and vegetable oil are added. Compared with the prior art, glucose and vegetable oil are adopted for blending in the fermentation process, and the acid yield is increased; meanwhile, corn protein powder serves as an organic nitrogen source, the protein content of the corn protein powder is high and reaches 50% or above, and high-density fermentation of gibberellin can be achieved; the hidden danger that aspergillus flavus is generated from peanut cake powder can beavoided, synthesis and metabolism of thalli are ensured, and the thalli can be rapidly and stably fermented; furthermore, the vegetable oil is added to provide a carbon source for fermentation, the phenomena of fermentation bubble escaping and the like can be effectively reduced, and the potential risks such as material loss and bacterial contamination are avoided; in addition, trace elements areadded into a fermentation culture medium so that the activity of various enzymes and metabolites in the microbial growth metabolism process can be comprehensively ensured.

The invention belongs to the technical field of culture of enterococcus faecalis and relates to a high-density fermentation medium for the enterococcus faecalis for feed and a fermentation method of the medium. The strategy that addition of sucrose is controlled according to pH feedback is adopted, and meanwhile, the concentration of lactic acid in fermentation broth is reduced, so that the feedback inhibition effect of lactic acid is relieved; the bacterium density of the enterococcus faecalis fermented by the aid of the high-density fermentation medium with the fermentation method of the medium is remarkably higher than that of enterococcus faecalis fermented with a common fermentation method, the number of living bacteria is not less than 10<10> cfu/mL and is 40 times or more that of enterococcus faecalis cultured by the aid of a common MRS medium, and high-density fermentation of the enterococcus faecalis is realized; thus, the separation cost of biomass can be reduced, the production cycle can be shortened, the production cost can be reduced, and the production efficiency can be improved.

Provided is a method for improving the yield of vitamin B12 based on regulation of an ammonia nitrogen index. In the fermentation process of vitamin B12, stage-by-stage batch feeding is carried out; in the earlier stage of fermentation, a yeast extract juice is refilled, so a certain content of ammonia nitrogen is maintained at a certain stage, so as to ensure the nitrogen source needed for growthand metabolism of bacteria. The stage-by-stage batch feeding is carried out in the fermentation process, the yeast extract juice is refilled in the earlier stage of fermentation, the concentration ofammonia nitrogen is controlled, the contradiction between the growth of the bacteria and biosynthesis of high-concentration ammonia nitrogen in the fermentation of vitamin B12 is solved, the nutritional components required for the growth and metabolism of the bacteria are ensured in the early stage of fermentation, the growth of bacteria is promoted, the phenomena of premature senescence and metabolic stagnation in the later period are avoided, and the fermentation unit of vitamin B12 is increased.

The invention belongs to the field of microbial fermentation, and more particularly relates to a method for preparing glutathione by fed-batch fermentation of Candida utilis. The method comprises: taking the Candida utilis SZU 07-01 as original strain, carrying out slant cultivation and seed cultivation on the original strain, and fermenting for at least 42 hour; and fermentation more particularly comprises the steps of: after expansion of cultivation, inoculating seeds into a fermentation medium according to 5-10% of inoculation quantity, and controlling the fermentation temperature within the range of 28-32 DEG C, the stirring rotation speed within the range of 250-300rpm and the ventilation capacity within the range of 0.5-1.5vvm; furthermore, when the fermentation is carried out for 10-12h, feeding the fermentation medium in batch; and manually adjusting the rotation speed and the ventilation capacity, and controlling the concentration of dissolved oxygen to be more than or equal to 35%. By adopting multinomial fed-batch fermentation way, on the basis of meeting the demand for nutrient substance of yeast cells, the method further improves the cell density and GSH yield, obviously reduces the fermentation time and increases the production efficiency.

The invention provides gene engineering antibacterial peptide and a preparation method and application thereof, which can solve the problems of complicated structure, low antibacterial capacity and not wide antibacterial spectrum in the prior art. The antibacterial peptide has the amino acid sequence of KWKLFKKIAGPKFLHSKKKFN. According to antibacterial characteristics of cecropinA and magainins, a novel antibacterial peptide amino acid sequence is independently designed. On the basis, a preferred codon of pichia pastoris is selected, a novel antibacterial peptide gene is artificially synthesized and is cloned in the pichia pastoris for expression, a novel antibacterial peptide recombinant yeast strain, the fermentation scale is amplified to the fermentation tank level, and the high-density fermentation and high-efficiency expression of the antibacterial peptide product are realized. Fermentation liquor is further purified and prepared into powder, liquid and other preparations of the antibacterial peptide. The preparations of the gene engineering antibacterial peptide can serve as a livestock feed additive or are used for controlling livestock diseases.

The invention belongs to the technical field of food microorganisms and discloses a method for performing high-density fermentation on bacillus aceticus. The method comprises the following steps: (1)performing bacterium activation; (2) performing shake-flask culture; (3) performing culture amplification of seed tanks; and (4) performing fermentation in fermentation tanks, namely inoculating a seed liquid obtained in the step (3) into culture mediums in the fermentation tanks according to an inoculation amount of 1-20%, performing culture under culture conditions that the stirring rotation speed is 100-300 rpm, the air introduction amount is 0.1-1.0 vvm, and the temperature is 28-30 DEG C, feeding 5-10 M of sodium hydroxide, controlling the pH value of a fermentation liquid to 5.5-6.0, feeding cell pulp and polypeptide-k, and culturing for 8-12 hours, wherein the cell pulp is cell pulp of bacillus aceticus. By adopting the method, the production cycle is greatly shortened, bacteria canbe relatively greatly enriched, and high-density fermentation of 1.24-3.29*10<9>cfu/mL is achieved.

The invention discloses a yeast and a method for producing a chromium-enriched yeast by utilizing the yeast via high-density fermentation, wherein the collection number of the yeast is CCTCC No. M2013238. The method for producing the chromium-enriched yeast comprises the following steps of: (1) inoculating the yeast with the collection number of CCTCC No. M2013238 on a culture medium, and performing high-density fermentation; (2) performing feedback feeding on nutrient substances by virtue of the combination of ethyl alcohol and dissolved oxygen; (3) controlling the concentration of inorganic chromium in a fermentation system at 50-200 mg/L by feeding inorganic chromium; (4) stopping fermentation after the yeast stops growth, and centrifuging, washing and drying to obtain the chromium-enriched yeast. By virtue of the yeast and the method disclosed by the invention, conversion for inorganic chromium can be much effectively realized, and the content of organic chromium can achieve 6500 ppm. Via the feedback feeding technology, high-density fermentation for the chromium-enriched yeast is realized, and the yield of wet yeasts is 250-320 g/L, thus effectively increasing production efficiency.

The invention belongs to the field of food biotechnology, and discloses a method for controlling a pH value and supplementing high-density fermentation acetobacter aceti in a double-feeding mode. Themethod comprises the following steps: (1) activating thallus; (2) performing shake-flask culture; (3) performing seed tank propagation; (4) performing fermentation tank fermentation: inoculating a seed solution obtained in step (3) on a culture medium in a fermentation tank to culture according to an inoculation amount of 1%-20%, wherein culture conditions are as follows: stirring rotation speed of 100-300 rpm, throughput of 0.1-1.0 vvm, a temperature of 28-30 DEG C; and feeding 5-10 M of sodium hydroxide, controlling the pH value of fermentation liquor to 5.5-6.0, and feeding yeast extract and corn pulp to culture for 8-12 hours. A way of controlling the pH value and adopting yeast extract and corn pulp to supplement in a double-feeding mode is adopted, so that the production period is greatly shortened, and therefore, more thallus can be enriched more quickly, and high-density fermentation of 1.56-3.34*10<-9> cfu/mL is realized.

The invention provides a fermented culture medium for producing gibberellic acid. The fermented culture medium comprises 10-40g/L of corn gluten meal, 1-10g/L of monopotassium phosphate, 5-30g/L of asmall molecule organic carbon source, 0.5-5g/L of plant oil, 0.5-2g/L of magnesium sulfate, 0.5-2g/L of ammonium sulfate and 0.1-1g/L of trace elements. Compared with the prior art, the fermented culture medium is relatively high in protein content since the corn gluten meal is adopted as an organic nitrogen source, that is, the protein content is up to 50% or greater, high-density fermentation ofgibberellins is achieved, the potential danger of aspergillus flavus of peanut cake powder is avoided, synthesis and metabolism of bacteria are ensured, and rapid and stable fermentation of the components can be achieved; due to adoption of the plant oil, not only is a carbon source for fermentation provided, but also situations of escape liquids of fermentation bubbles, and the like, are effectively reduced; due to addition of the trace elements in the fermented culture medium, activity of various enzymes and metabolite in the microorganism growth and metabolism process is comprehensively ensured.

The invention provides a preparation method of gibberellic acid. The preparation method comprises the following steps of (S1) preparing gibberellic acid seed liquid; (S2) transferring the gibberellicacid seed liquid into a fermentation tank to be fermented for obtaining gibberellic acid fermentation liquid; S3, performing filter filtering concentration on the gibberellic acid fermentation liquidthrough film filtering to obtain concentration liquid; (S4) refining the concentration liquid to obtain the gibberellic acid. Compared with the prior art, the method has the advantages that the sugarand oil mixed supplementation is used in the fermentation process; the acid yield is improved; meanwhile, corn protein powder is used as an organic nitrogen source; the protein content is relatively high and reaches 50 percent or higher; the high-density fermentation of the gibberellin can be realized; the potential danger of aspergillus flavus generated by peanut meal can be avoided; the thallussynthesis and metabolism are ensured; the fast and stable fermentation can be realized; in addition, plant oil is added, so that a carbon source is provided for the fermentation; the occurrence of conditions of fermentation bubble liquid escape and the like can be effectively reduced; the potential risks of material loss, germ contamination and the like can be avoided.