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High-density fermentation culture medium formula for saccharomyces cerevisiae for feed and applications thereof

A technology of high-density fermentation and medium formulation, which is applied in the field of high-density fermentation medium formulation of Saccharomyces cerevisiae for feed, and can solve the problems of reducing fermentation cost and low concentration of yeast fermentation cells

Active Publication Date: 2015-03-11
HUNAN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The invention can ensure the vigorous growth of Saccharomyces cerevisiae, reduce the fermentation cost, and solve the problem that the concentration of yeast fermentation cells is not high at present

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Example 1 Configuration of fermentation medium and comparison of fermentation results

[0019] Saccharomyces cerevisiae high-density fermentation medium composition: Per liter, starch 20g / L, sucrose 5g / L, beef extract 10g / L, urea 10g / L, yeast extract 5g / L.

[0020] The medium preparation method is as follows:

[0021] Weigh each component according to the composition of the culture medium and dissolve in distilled water;

[0022] Put the prepared medium into the fermenter, stir and mix well, and sterilize at 121°C for 15 minutes;

[0023] In order to achieve the best effect of the medium, when using this medium to ferment Saccharomyces cerevisiae, the fermentation process is as follows:

[0024] The main steps of high-density fermentation are as follows:

[0025] 1) Activate cryopreserved Saccharomyces cerevisiae (CGMCC Collection No.: CGMCC No. 9436) on a YEPD agar plate, pick a single bacterium and inoculate it into a liquid test tube of this medium, After cult...

Embodiment 2

[0040] Example 2 Configuration of fermentation medium and comparison of fermentation results

[0041] Saccharomyces cerevisiae high-density fermentation medium composition: per liter, starch 22g / L, sucrose 5g / L, beef extract 10g / L, urea 10g / L, yeast extract 5g / L.

[0042] The medium preparation method is as follows:

[0043] Weigh each component according to the composition of the culture medium and dissolve in distilled water;

[0044] Put the prepared medium into the fermenter, stir and mix well, and sterilize at 121°C for 15 minutes;

[0045] In order to achieve the best effect of the medium, when using this medium to ferment Saccharomyces cerevisiae, the fermentation process is as follows:

[0046] The main steps of high-density fermentation are as follows:

[0047] 1) Activate the cryopreserved Saccharomyces cerevisiae on the YEPD agar plate, pick a single bacterium and inoculate it in the liquid test tube of this medium, cultivate it at 25°C for 12 hours, and transfe...

Embodiment 3

[0061] Example 3 Configuration of fermentation medium and comparison of fermentation results

[0062] 1. Composition of Saccharomyces cerevisiae high-density fermentation medium: per liter, starch 18g / L, sucrose 5g / L, beef extract 10g / L, urea 10g / L, yeast extract 5g / L.

[0063] 2. The medium preparation method is as follows:

[0064] 1) Weigh each component according to the composition of the culture medium and dissolve in distilled water;

[0065] 2) Put the prepared medium into the fermenter, stir and mix well, and sterilize at 121°C for 15 minutes;

[0066] In order to achieve the best effect of the medium, when using this medium to ferment Saccharomyces cerevisiae, the fermentation process is as follows:

[0067] 1. The main steps of high-density fermentation are as follows:

[0068] 1) Activate the cryopreserved Saccharomyces cerevisiae on the YEPD agar plate, pick a single bacterium and inoculate it into the liquid test tube of this medium, and after culturing at ...

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Abstract

The invention belongs to the technical field of agricultural microbes, and relates to a high-density fermentation culture medium formula for saccharomyces cerevisiae for feed and applications thereof. The culture medium is composed of starch with a concentration of 18 to 22 g / L, cane sugar with a concentration of 5 g / L, beef extract with a concentration of 10 g / L, urea with a concentration of 10 g / L, and yeast extract with a concentration of 5 g / L. The fermentation technology conditions are as follows: temperature is 25 DEG C, pH value is 6 to 6.5, and the fermentation is performed for 20 to 24 hours in a primary throughput of 1.2:1. Two to three hours after the absorption value reaches 1.4 under the waves with an OD value of 600 nm, liquid is supplemented according to a volume ratio of 1:10. The supplement liquid is composed of starch with a concentration of 100 g / L, cane sugar with a concentration of 25 g / L, beef extract with a concentration of 50 g / L, urea with a concentration of 50 g / L, and yeast extract with a concentration of 25 g / L. The fermentation density (live bacterium number) of saccharomyces cerevisiae in a provided culture medium is increased by 7.3 times compared to that of saccharomyces cerevisiae in a YPED culture medium. The bacterium concentration reaches 1.05*109 cfu / mL, and the dry bacterium weight reaches 112 g / L. The using amount of peptone and glucose is reduced, thus the production cost is reduced, and the culture medium is more suitable for industrial production.

Description

technical field [0001] The invention belongs to the technical field of agricultural microbes, and relates to a high-density fermentation medium formula of Saccharomyces cerevisiae for feed and an application thereof. Background technique [0002] With the development of animal husbandry production, animals have an increasing demand for protein, but the total amount of agricultural production is limited, and the demand for protein feed necessary for growth is increasing. The nutritional value of Saccharomyces cerevisiae protein is very high. The protein content in yeast dry matter can be as high as 50%, and the content of lysine and arginine is relatively high, which is close to animal protein. The content of tryptophan is more than 7 times higher than that of soybean. In addition, Saccharomyces cerevisiae is also rich in nucleotides, B vitamins, minerals and other physiologically active substances, which are suitable for addition as high-protein active yeast feed. With the ...

Claims

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Application Information

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IPC IPC(8): C12N1/16A23K1/16C12R1/865
CPCC12N1/16
Inventor 方俊符晨星卢向阳
Owner HUNAN AGRICULTURAL UNIV
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