220 results about "Yeast fermentation" patented technology

The invention belongs to the technical field of biology, and relates to a combined method for improving the taste of cerasus humilis clear juice and increasing the content of available calcium and zinc, which comprises the following operation steps: S1, removing impurities from cerasus humilis fruits, cleaning, denucleating and crushing to obtain cerasus humilis pulp; s2, performing primary enzymolysis on the cerasus humilis fruit pulp to obtain primary enzymolysis cerasus humilis fruit pulp; s3, fermenting the cerasus humilis fruit pulp to obtain fermented cerasus humilis fruit pulp; s4, performing secondary enzymolysis on the fermented cerasus humilis fruit pulp to obtain secondary enzymolysis cerasus humilis fruit pulp; and S5, filtering, sterilizing and filling the secondary enzymolysis cerasus humilis pulp to obtain a cerasus humilis clear juice product. The combined method of high-speed dispersion machine release, pectinase enzymolysis, issatchenkia terricola fermentation and joint enzymolysis of cellulase, beta-glucosidase and alpha-rhamnosidase is utilized, the product taste is effectively improved while the yield of the cerasus humilis clear juice product is increased, and the cerasus humilis clear juice product is palatable in sourness and sweetness, clear, transparent and bright, full in flavor and suitable for large-scale production. And the contents of calcium and zinc are increased by more than 65% and 59%. The product has good calcium and zinc supplementing effects.

The invention discloses a Raman spectrum-based online detection method for glucose and ethanol in a yeast fermentation process. The Raman spectrum-based online detection method specifically comprises the following steps: preparing a yeast seed solution, and inoculating the yeast seed solution into a fermentation tank for fermentation; in the fermentation process, an immersion type Raman optical fiber probe is used for collecting a Raman spectrum in real time, and meanwhile sampling is conducted at regular time to measure the concentration of glucose and ethanol; sequentially carrying out median filtering, adaptive iteration reweighted penalty least square method (airPLS) baseline correction, Savitz-golay (SG) smoothing processing and characteristic peak normalization on the acquired spectrum; respectively establishing concentration prediction models of glucose and ethanol by adopting a PLS1 algorithm; and applying the model to a subsequent batch fermentation process to realize online prediction of glucose and ethanol. According to the method, manual sampling is not needed, prediction is accurate, operation is easy and convenient, and in-situ online detection of glucose and ethyl alcohol in the yeast fermentation engineering can be achieved.

The invention provides a clostridium endophyllum engineering bacterium as well as a construction method and application thereof, and belongs to the technical field of biology, compared with a wild type strain, the clostridium endophyllum engineering bacterium can only produce acetic acid in a low-pH environment under the condition of taking CO2 and H2 as raw materials, and does not generate by-products such as ethanol, lactic acid, 2, 3-butanediol and the like. Besides, the fermentation liquor of the clostridium endophyllum engineering bacteria can be directly used as an acidic substrate to adjust the pH value of downstream yeast fermentation liquor, meanwhile, automatic addition of a carbon source is achieved, zero addition of inorganic salt in the cascade conversion process from CO2 and H2 to grease or yeast protein is achieved, and the economical efficiency and environmental friendliness of the whole process are improved.

The invention provides anti-aging moisturizing essence and a preparation method thereof, and belongs to the technical field of cosmetics. The essence is prepared from a plant extract, sodium pyrrolidone carboxylate, hexapeptide, nicotinamide mononucleotide, vitamin B5, galactose yeast fermentation product filtrate, b-glucan, sodium hyaluronate, glycerol, tranexamic acid, blue copper peptide, arbutin, nicotinamide, vitrin, a bifidus yeast fermentation product, a bifidus yeast fermentation lysate and the like. Wherein the plant extract is prepared by mixing rosemary, frankincense resin, tea flowers, sandalwood and jasmine flowers through ultrasonic extraction, comprehensive skin care is achieved from multiple angles of anti-aging, whitening, moisturizing and repairing, the overall health of the skin is guaranteed, and the skin care cream is suitable for various skin types.

The application belongs to the field of daily chemicals, and discloses a Bifidobacterium adolescentis, the classification name of the Bifidobacterium adolescentis BF23BA001 is Bifidobacterium adolescentis, the preservation number is CGMCC NO.26999, the preservation date is March 31, 2023, the preservation unit is China General Microbiological Culture Collection Center, and the preservation address is No.3, Xibahe Road, Chaoyang District, Beijing City. Compared with other similar bacteria on the market, the bacteria have unique advantages in the production of small molecule peptides and gamma-aminobutyric acid, according to the functional characteristics of the small molecule peptides and gamma-aminobutyric acid, the bacteria have significant anti-wrinkle advantages, and through subsequent functional verification, the bacteria have very important significance for further improving the whitening, brightening, repair and anti-aging effects of yeast fermentation products. In addition, the application also discloses a composition for inhibiting the generation and accumulation of lipofuscin, application based on the composition and cosmetics.

The invention discloses a method for extracting retinol from yeast fermentation liquor. The method comprises the following steps: A, extracting the fermentation liquor to obtain an extraction phase; step B, concentrating the extract phase to obtain concentrated oil; step C, purifying concentrated oil; and optionally step D, concentrating the purified eluent.

The invention relates to an anti-wrinkle and anti-aging composition and application thereof. The anti-wrinkle and anti-aging composition is prepared from hydrolyzed rice protein, a bifidus yeast fermentation product lysate, dipeptide diaminobutyryl benzyl amide diacetate, adenosine, hydroxydecyl ubiquinone, ceramide NP, palmitoyl tripeptide-5, carnosine and acetyl hexapeptide-8. According to the present invention, the components achieve the synergistic effect, such that the fibroblast proliferation can be promoted, the cell metabolism can be improved, the generation of collagen, elastin and the like can be promoted, the excellent oxidation resistance can be provided, and the effects of expression line inhibition, existing wrinkle fading and long-term aging resistance can be combined.

The invention discloses a composition with effects of removing dandruff, inhibiting bacteria and relieving itching as well as a preparation method and application thereof, and belongs to the technical field of daily chemicals. The composition is compounded with a yeast fermentation lysate filtrate, a bacillus fermentation product, a fructus kochiae extract, a red clover extract and a cacumen biotae extract; through a'micro-ecology-barrier-inflammation 'triple synergistic path, the synergistic interaction of inhibiting bacteria more quickly, relieving itching for a longer time and removing dandruff more thoroughly is realized. The relative contents of the substances are optimized, so that the efficacy is maximized, and the stability of the composition is ensured. The composition can be used as a cosmetic raw material to be applied to scalp stability maintaining essence, has a good effect of improving the scalp microenvironment, and is mild and non-irritant.

The invention discloses an extraction method and application of lavender essential oil based on yeast fermentation pretreatment, and belongs to the technical field of plant active ingredient extraction. The method comprises the following steps: activating saccharomycetes, inoculating the saccharomycetes into a mixture containing a crushed lavender raw material and water, and fermenting at 30-38 DEG C for 20-28 hours to obtain fermentation liquor; then sterilizing and centrifuging the fermentation liquor to obtain supernate; and finally extracting the essential oil through steam distillation. Through yeast fermentation pretreatment, the lavender cell wall structure is effectively destroyed, essential oil release and active ingredient conversion are promoted, and the essential oil yield and antioxidant activity are remarkably improved. Compared with a traditional steam distillation method, the method has the advantages that the essential oil yield can be increased from 0.55% to 0.75% or above, and the DPPH free radical scavenging rate can be increased to 51.75% or above. The method is green in process and simple and convenient to operate, a new way is provided for high-value utilization of lavender resources, and the prepared essential oil can be used for developing cosmetics with anti-oxidation and anti-aging effects.

The invention relates to the technical field of animal feed, in particular to cold-season yak supplementary feeding granules and a supplementary feeding method and application thereof. The supplementary feeding granular material for yaks in the cold season is prepared from the following components in percentage by weight: 10-13% of corn straws, 20-25% of sunflower seed shells, 35-38% of corn kernels, 9.4-10% of soybean meal, 8-10% of cottonseed meal, 0.8-1% of baking soda, 0.8-1% of table salt, 0.5-1% of feed premix and 4-5% of yeast leavening. Through a scientific formula, various nutritional ingredients needed by yaks can be provided, it is ensured that the yaks obtain comprehensive nutritional support in different growth stages, and meanwhile, high-energy nutritional ingredients can be provided to make up for a large amount of energy consumed by the yaks in the growth process; the feed can be converted into meat more efficiently, the growth speed and production benefits of the yaks are improved, and the feed has good digestibility and can be better absorbed by the yaks, so that feed waste is reduced, and the feeding efficiency is improved.

The invention belongs to the field of cosmetics, and discloses an anti-wrinkle soothing composition, application of the anti-wrinkle soothing composition and a cosmetic. Comprising the following components in parts by weight: 15-20 parts of hydroxypropyl tetrahydropyrantriol, 10-15 parts of tetrahydromethylpyrimidine carboxylic acid, 5-10 parts of a leukocyte extract, 5-20 parts of a leontopodium alpinum callus extract solution and the balance of a bifidus yeast fermentation product filtrate, through reasonable compounding of the five components, synthesis of collagen and elastin can be effectively promoted, and good anti-wrinkle and soothing effects are achieved.

The invention relates to the field of cosmetics, in particular to a blue copper peptide composition with instantaneous cool feeling and infiltration promotion effects and a preparation method and application of the blue copper peptide composition. The composition comprises an active component copper-blue peptide and a cooling and permeation enhancing system formed by compounding at least two of menthane formylethylamine, menthone glyceryl ketal and methyl diisopropyl propionamide, and the active component can further comprise hexapeptide-11, a blue algae extract and a yeast fermentation product. The preparation method of the composition comprises three core steps of providing a water phase, adding the active ingredients and adding the cool and refreshing infiltration promoting system and other components. Through synergistic compounding of specific fresheners, on the premise that the use amount of each single component is remarkably reduced, efficient permeation promotion of the copper-blue peptide is achieved, the layered cool feeling experience which takes effect quickly and is lasting and mild is provided, the common skin irritation problem of a traditional permeation promoter or a high-concentration single freshener is avoided, and the effect is remarkable. And the transdermal patch has excellent transdermal efficiency, active matter stability and use comfort.

The invention relates to the technical field of daily chemicals, in particular to a soothing composition as well as a preparation method and application thereof. The composition is prepared from saccharomycetes fermentation lysate filtrate, peony root extract, spanishneedles herb extract, liquorice root extract, glycerol and tremella fuciformis extract. Small molecule peptides in the fermentation lysate and flavone, polyphenol and triterpenoid components in the plant extract can inhibit inflammation pathways (such as TNF-alpha, IL-6 and COX-2) in a multi-target mode, and skin redness is effectively relieved. The skin barrier repairing capability is high: the tremella acidic polysaccharide and the yeast polypeptide form a synergistic network, so that the water content of a cuticle is favorably enhanced, and barrier lipid synthesis is promoted; a polysaccharide-polypeptide cross-linked chain structure is broken through a high-shear homogenizing treatment technology, so that the wiredrawing and colloid decorous feeling of a finished product are remarkably reduced, and a refreshing skin feeling is obtained.

The invention relates to the technical field of cosmetics, and discloses an umbilical cord blood moisturizing essence composition, an umbilical cord blood moisturizing essence as well as a preparation method and application of the umbilical cord blood moisturizing essence. The composition contains a hydrolyzed placenta extract, placenta protein, an umbilical cord extract, a guava fruit extract, a radix gentianae extract, decapeptide-4, nonapeptide-1, a lactobacillus fermentation product and a yeast fermentation product filtrate. The umbilical cord blood moisturizing essence prepared from the composition has multiple effects of moisturizing, repairing and resisting aging, and is mild, safe, excellent in stability and high in active ingredient retention rate.

The invention belongs to the technical field of intelligent brewing of baijiu, and discloses a multi-omics-based intelligent domestication system and method for a Maotai-flavor baijiu producing yeast group, and the method comprises the following steps: collecting multi-source data, constructing a multi-dimensional data framework, correcting space-time delay of a sensor, and generating an environment-metabolism associated data set; defining a response threshold value of each partition to perform partition environment risk assessment, and constructing a three-dimensional responsibility library; judging the risk state of the responsibility partition by combining the symbiotic efficiency index, and generating a flora binding rule table; dividing conflict interception levels, and generating a three-dimensional topology; four-stage structured gate interception is carried out, and a dynamic domestication instruction set is generated; analyzing flora response deviation by simulating a layered stress scene, and positioning a deviation source in combination with transcription-metabonomics traceability; the efficiency base line and layering strategy container parameters are dynamically updated, the problems of low yeast fermentation efficiency, poor stress resistance, extensive flavor regulation and control and the like in a traditional process are solved, and dynamic optimization of microbial communities and system self-adaptive capacity are achieved.

The invention relates to the technical field of yeast fermentation monitoring, in particular to a real-time monitoring method for microfermentation of high-temperature-resistant yeast. The method comprises the following steps: obtaining a matched fermentation sub-time period of a monitoring time period under each sample yeast, and obtaining a matched environment sequence corresponding to a current target environment parameter sequence under each sample yeast according to the matched fermentation sub-time period of the monitoring time period and a sample target environment parameter sequence; according to the deviation between the current target environment parameter sequence and a matching environment sequence corresponding to the current target environment parameter sequence and the fermentation quality score, obtaining an abnormal characterization value of each environment parameter type at the current fermentation monitoring moment; monitoring the environmental parameters of the target yeast at the current fermentation monitoring moment according to the abnormal characterization value; and the timeliness and the accuracy of real-time monitoring of the microfermentation process of the high-temperature-resistant saccharomycetes can be improved, so that the stability and the fermentation quality of the fermentation process can be improved.

This invention belongs to the field of alcoholic beverage processing technology, specifically relating to a high-purity ginkgo wine and its preparation method. The preparation method comprises the following steps: ginkgo slurry undergoes preliminary enzymatic hydrolysis with neutral protease and amylase, followed by secondary enzymatic hydrolysis with pectinase and sodium bisulfite; brewing yeast fermentation broth is added for pre-fermentation; glutamine transaminase, *Saccharomyces cerevisiae* fermentation broth, and *Candida valerate* fermentation broth are added for post-fermentation; and finally, distillation and filtration are performed to obtain the ginkgo wine. This invention achieves complete decomposition and fermentation of ginkgo kernels through a two-step enzymatic hydrolysis and two-step fermentation process. The preparation method has advantages such as simple process and low cost, which is beneficial for industrial production; the prepared ginkgo wine has advantages such as clarity, strong aroma, high alcohol content, and low acidity.

The invention discloses a sextuple yeast composition which comprises a bifidus yeast fermentation product lysate, a saccharomycetes / acetobacter xylinum / black tea fermentation product, a saccharomycetes / zinc fermentation product, galactose yeast-like bacterium fermentation product filtrate, a bifidus yeast fermentation product filtrate and a saccharomycetes / rice fermentation product filtrate in a mass ratio of 1: (1-1.2): (1-1.2): (4-6): (32-35): (160-168). According to the sextuple yeast composition disclosed by the invention, sextuple yeast fermentation products generate a synergistic effect, so that multiple effects of moisturizing, repairing, brightening, oil control, oxidation resistance and the like are realized, and the skin state is comprehensively improved. Long-term use can enhance skin barrier, reduce fine wrinkles, and improve skin gloss and elasticity.

A method for high-density fermentation production of ULP involves culturing yeast containing a ULP expression vector in a basal medium comprising a carbon source (glycerol) and a nitrogen source (histidine and / or ammonium dihydrogen phosphate). This invention, through high-density yeast fermentation and employing an improved culture medium and process, significantly improves the yield and quality of ULP. The final expression level is 3-5 times higher than that achieved by conventional E. coli fermentation methods, reducing ULP production costs by at least 25%. This reduces the cost of related recombinant protein drugs and allows for wider application of this high-quality system in recombinant protein drug development.

The present invention discloses a carbon dioxide recovery device for a yeast fermentation tank, comprising a carbon dioxide collecting tank, a spray bin installed on one side of the carbon dioxide collecting tank, a dehumidification bin installed on the top of the outer side of the carbon dioxide collecting tank, and an air suction pump provided on the carbon dioxide collecting tank, the input end of the air suction pump being connected to the top of the spray bin, and the output end of the air suction pump being connected to the bottom of the dehumidification bin; the present invention processes carbon dioxide by spraying and dehumidifying fillers, thereby improving the quality of carbon dioxide recovery, and is not operated by an independent tank body, thereby reducing the space occupied by the equipment; through the mutual cooperation of a rotating rod, a stirring blade, a gear, an inner gear ring and a shifting rod, the combination of the shifting rod and the inner gear ring is similar to a ship's rudder, and can be rotated from multiple directions, and a group of inner gear rings synchronously drive multiple groups of rotating rods and stirring blades to rotate, thereby stirring the hygroscopic filler to prevent it from agglomerating, thereby ensuring a good dehumidification effect.

The invention relates to the technical field of biology, in particular to yeast hydrolysate and application thereof. According to the method, enzymes are reasonably screened, the obtained compound enzyme can conduct enzymolysis on yeast strains more effectively, so that hydrolysate rich in free-state lipid, amino acid, polysaccharide and other substances is prepared, the hydrolysate is more beneficial to growth and utilization of yeast, the health state of active yeast cell membranes is improved, and yeast fermentation is promoted.

The application discloses a fermented fruit juice with the effect of reducing high uric acid and a preparation method thereof, and comprises the following steps: step one, obtaining fruit wine after fruit juice fermentation, and obtaining de-alcohol fruit juice by removing alcohol from the fruit wine; step two, inoculating lactic acid bacteria into the de-alcohol fruit juice to perform lactic acid fermentation, and obtaining the fermented fruit juice; the lactic acid bacteria comprises lactobacillus helveticus CICC6064 and / or lactobacillus paracasei CICC20292. The fruit juice prepared by the fermentation process disclosed in the application not only retains the metabolic products of the yeast fermentation except for alcohol, but also increases the biomass and improves the content of apple polyphenol monomers through lactic acid bacteria fermentation, so that the fermented apple juice has the effects of rich taste and nutrition and reducing high uric acid, and has the innovation in the field of functional development of ordinary fruit juice.

The invention belongs to the field of microbial fermentation and the technical field of daily chemicals, and particularly relates to deacetylated sophorolipid as well as a preparation method and application thereof. By using cheap culture medium components and screening out saccharomycetes with high yield of lactone type sophorolipid, the problem that the production cost of the sophorolipid is too high is effectively solved, and the application of the sophorolipid in various fields is promoted; through the step of carrying out enzymolysis on a fermentation substrate in advance, the problems of long single fermentation period, low thallus utilization rate, high energy consumption and high cost of traditional saccharomycetes are solved. A multi-round fermentation circulation system is designed, and bacteria liquid is settled and recycled and then inoculated and fermented, so that thalli are efficiently and repeatedly utilized, the unit yield is increased, the fermentation period is shortened, and the production cost is effectively controlled. The structure is adjusted through a resin modification technology, a final product mainly comprises acetylated sophorolipid, and compared with acetylated sophorolipid and deacetylated sophorolipid, the sophorolipid has better foam stability and sensitive skin repairing effect and is suitable for the application fields of cosmetics, washing care and the like.

This invention provides a method for the separation, purification, and aroma quality control of biosynthesized patchouli essential oil, belonging to the field of daily chemical product purification technology. It solves the technical problems of low separation efficiency, insufficient purity, easy destruction of heat-sensitive components, and low aroma matching in existing essential oils. This method uses metabolically engineered brewer's yeast fermentation broth as raw material, sequentially undergoing pretreatment, multi-stage countercurrent extraction, three-stage high-vacuum distillation, column chromatography purification, activated carbon decolorization and deodorization, aroma component recombination, and product stabilization to obtain high-purity patchouli essential oil with high aroma matching. This invention is adapted to the three-stage distillation process of biosynthetic systems, achieving efficient separation of solvents, sesquiterpenes, and patchouli alcohol, resulting in high purity; the product aroma has a high matching degree with natural Indonesian essential oils; the process is mild throughout, can be stably scaled up from small-scale to ton-scale, and is low-cost. This invention provides a complete, efficient, and green technical solution for the industrialization of natural fragrances derived from synthetic biology.

This invention provides a method for yeast fermentation of recombinant batroxobin and the culture medium used, the fermentation process mainly comprising primary seed culture, secondary seed culture, and fermentation culture. In the fermentation culture stage, the culture medium is optimized by adding multiple nutrients such as glycerol, casein amino acids, and PTM1 inorganic salts, and the pH of the fermentation medium is appropriately adjusted. The activity of the fermented recombinant protein is improved by methanol induction culture. The components of the optimized base fermentation medium are: K2SO4 0.91%, MgSO4 0.36%, CaSO4·2H2O 0.059%, 85% H3PO4 2.5% (V / V), KOH 0.206%, glycerol 4%, defoamer 0.05%~0.1%, PTM1 0.4% (V / V), casein amino acids 0.5%. The components of the optimized glycerol-added medium are: 50% glycerol + 12 mL / L PTM1 + 0.5% casein amino acids. The components of the optimized methanol induction medium are: (100% methanol + 12 mL / L PTM1) 95% + 4.5% sorbitol + 0.5% Casein is an amino acid.

Provided is a method for producing a yeast fermentation preparation with which an aroma component can be produced more efficiently. A method for producing a yeast fermentation preparation, which is characterized by comprising a step for fermenting a fermentation raw material obtained by adding butyric acid or a salt thereof to a milk-derived raw material by using a yeast. Also disclosed is a method for producing a yeast fermentation preparation by adding benzoic acid or a salt thereof to a fermentation starting material thereof. It is preferable that Wickerhamomyces pijperi be used as the yeast, and it is also preferable that Wickerhamomyces pijperi be used as the yeast.

The invention provides a preparation method of red date wine and the red date wine, and relates to the technical field of wine brewing. The preparation method of the red date wine comprises the following steps: S1, mixing sterilized and enzyme-deactivated enzymolysis red date juice with lactic acid bacteria, and performing lactic acid bacteria fermentation to obtain fermentation liquor; s2, mixing the fermentation liquor with saccharomycetes, and performing saccharomycetes fermentation to obtain red date wine; the lactic acid bacteria in the S1 are alcohol-tolerant lactic acid bacteria, and the saccharomycetes in the S2 are BV818 saccharomycetes; the fermentation liquor in the step S2 is not sterilized before being mixed with the saccharomycetes. According to the preparation method of the red date wine, damage to heat-sensitive functional components such as polyphenols and flavones in the red dates is avoided, microbial fermentation is more sufficient, low alcohol is achieved, the content of the functional components and the oxidation resistance are reserved and improved, the red date wine further contains rich flavor substances, and the taste of the red date wine is improved. Pure natural fruity flavor and fine and soft taste of the wine body are guaranteed, and the comprehensive quality of the product is excellent.

This invention belongs to the field of cosmetic technology, specifically relating to an anti-aging composition and its application. The anti-aging composition provided by this invention comprises the following raw materials in parts by weight: 4-10 parts of tea / yeast fermentation product, 3-12 parts of white truffle extract, and 1-6 parts of red algae extract. The anti-aging composition provided by this invention selects tea / yeast fermentation product, white truffle extract, and red algae extract as active ingredients to synergistically enhance the anti-aging effect of the composition.

This invention discloses a skin-whitening and brightening composition and its application. The composition comprises a soft mask powder and a mask-mixing liquid, wherein the liquid comprises glycerin, 1,4-butanediol, Portulaca oleracea extract, yeast fermentation product filtrate, 1,2-hexanediol, p-hydroxyacetophenone, glyceryl acrylate / acrylic acid copolymer, PVM / MA copolymer, Camellia japonica flower extract, Prunus yedoensis leaf extract, nonapeptide-1, tanshinone volatile oil, a skin-whitening and melanin-reducing agent, and water. This composition can be used directly as a face mask. Compared with existing technologies, the skin-whitening and brightening composition prepared by this invention has the advantages of good whitening effect and good transdermal absorption.

This invention relates to the field of cosmetics, specifically to a composition containing copper peptides for repairing photothermal damage and its application. The composition comprises a complex polypeptide, galactosomal yeast fermentation product filtrate, and cyanobacterial extract; wherein the complex polypeptide includes copper peptides, yeast polypeptides, and hexapeptide-11. This invention, by combining specific complex polypeptides with galactosomal yeast fermentation product filtrate and cyanobacterial extract, increases the expression level of heat shock proteins, thereby achieving excellent efficacy in repairing photothermal damage. Furthermore, this composition is mild and non-irritating, and can be widely used in cosmetics.