High-purity isomaltose hypgather and alcohol co-production preparation method
A technology of isomaltooligosaccharide and isomaltose, which is applied in the field of joint production of high-purity 90% isomaltooligosaccharide and alcohol, can solve the problems of difficult recovery of alcohol, low content of functional components, and expensive enzymes, etc., and achieves The effect of improving product quality, improving quality and value
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Embodiment 1
[0031] (1) pulping and liquefaction
[0032] Take 100kg of cornstarch, adjust the concentration to 17.5°Be' with water, adjust the pH value to 6.0, add 45ml of high-temperature-resistant α-amylase, and carry out continuous liquefaction for 100 minutes, and the liquefaction DE value is 14%.
[0033] (2) Saccharification
[0034] Cool the liquefied solution to 59°C, adjust the pH to 4.9, add 80ml of compound glucoamylase (Genencor OPTIMAXVHP4060), maintain the temperature at 58-60°C, stir intermittently, and measure the composition after 20 hours of incubation, glucose 96%. Enzyme treatment.
[0035] (3) Filtration, concentration
[0036]The temperature of the saccharification solution was raised to 80°C, 1kg of activated carbon was added, stirred at constant temperature for 0.3h, plate and frame filtered, and the filtered sugar solution was concentrated to 65% in a plate evaporator.
[0037] (4) Conversion
[0038] Adjust the pH of the concentrated saccharification solution...
Embodiment 2
[0048] (1) pulping and liquefaction
[0049] Take 150kg of cornstarch, adjust the concentration to 17.5°Be' with water, adjust the pH value to 6.0, add 70ml of high-temperature-resistant α-amylase, and carry out continuous liquefaction for 95 minutes, and the liquefaction DE value is 13%.
[0050] (2) Saccharification
[0051] Cool the liquefied solution to 58°C, adjust the pH to 4.8, add 120ml of compound glucoamylase Genencor OPTIMAXVHP4060, maintain the temperature at 58-60°C, stir intermittently, and measure the enzyme inactivation treatment after 20 hours of incubation.
[0052] (3) Filtration, concentration
[0053] The saccharification solution was heated to 80°C, 1.5kg of activated carbon was added, stirred at constant temperature for 0.3h, plate and frame filtered, and the filtered sugar solution was concentrated to 65% in a plate evaporator.
[0054] (4) Conversion
[0055] Adjust the pH of the concentrated saccharification solution to 4.2, add 1.5L of glucoamylas...
Embodiment 3
[0065] (1) pulping and liquefaction
[0066] Take 150kg of cornstarch, adjust the concentration to 17.5°Be' with water, adjust the pH value to 5.9, add 70ml of high-temperature-resistant α-amylase, and carry out continuous liquefaction for 93 minutes, and the liquefaction DE value is 12%.
[0067] (2) Saccharification
[0068] Cool the liquefied solution to 58°C, adjust the pH to 4.8, add 120ml of transglycosidase produced by Japan Amano Co., Ltd., maintain the temperature at 58-60°C, stir intermittently, and measure the enzyme inactivation treatment after 20 hours of incubation.
[0069] (3) Filtration, concentration
[0070] The saccharification solution was heated to 80°C, 1.5kg of activated carbon was added, stirred at constant temperature for 0.3h, plate and frame filtered, and the filtered sugar solution was concentrated to 65% in a plate evaporator.
[0071] (4) Conversion
[0072] Adjust the pH of the concentrated saccharified solution to 4.2, add 1.5L glucoamylase ...
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