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Method for producing bacterial cellulose by taking citrus pomace as raw material

A technology of bacterial cellulose and citrus pomace, applied in the direction of microorganism-based methods, biochemical equipment and methods, bacteria, etc., can solve the problems of underutilization of secondary fruits, low commodity utilization rate, environmental pollution, etc., and achieve Improve the site and equipment, high output, good flexibility

Active Publication Date: 2011-08-31
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Nearly 20-30% of pomace will be produced when citrus is used for fruit juice and other processing, most of which were discarded directly as waste in the past; in addition, there are still a large number of residual and secondary fruits with extremely low commodity utilization rate that cannot be fully utilized. Caused a huge waste of resources and environmental pollution

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Embodiment 1: (with Wenzhou Satsuma pomace as raw material production method 1 of bacterial cellulose)

[0049] (1) Pretreatment of citrus pomace: After scalding, peeling, and juicing of Wenzhou satsuma citrus, the pomace was mixed with water in a ratio of 1:2 by weight, beaten to prepare pomace pulp, and pectinase was added to 2500U / mL After 2 hours of treatment, filter with an 800-mesh filter cloth, take the filtrate, adjust the pH to 4.0, and sterilize at 121°C for 15 minutes to form a citrus pomace pretreatment liquid for later use;

[0050] (2) Yeast fermentation of citrus pomace pretreatment liquid: preheat sugar water containing 30g / L sugar to 38°C, mix with Saccharomyces cerevisiae high activity dry yeast at a volume weight ratio of 20mL:1g, stir and keep warm for 20min, wait for temperature After cooling down to ≤34°C, keep it for 1.5h to form a yeast seed liquid; add the yeast seed liquid into step (1) citrus pomace pretreatment liquid according to the inoculu...

Embodiment 2

[0054] Embodiment 2: (with navel orange pomace as the method 2 of raw material production bacterial cellulose)

[0055] In this example, in step (1), the navel orange fruit is blanched, peeled, and squeezed, and then the pomace is mixed with water in a ratio of 1:15 by weight, and beaten to prepare a pomace slurry. After adding pectinase to 500U / mL for 2 hours , take the filtrate and add sucrose to 200g / L, adjust the pH value to 6.5, and sterilize it for later use; step (2) prepare the wine yeast into yeast seed liquid, inoculate according to the inoculation amount of 5mL / L, and culture at 30°C for 2 days to stop fermentation. Centrifuge to get the supernatant; step (3) add yeast powder 15g / L, sucrose 120g / L, ammonium sulfate 15g / L in the supernatant, adjust the pH value to 6.5, boil and sterilize; step (4) press the seed liquid by 150mL / L inoculum amount was inserted into the production fermentation culture medium, the thickness of the culture medium was 5cm, and cultured at...

Embodiment 3

[0056] Embodiment 3: (the method 3 that takes pomelo pomace as raw material to produce bacterial cellulose)

[0057]In this example, in step (1), the pomace fruit is blanched, peeled, and squeezed, and then the pomace is mixed with water in a ratio of 1:10 by weight, beaten to prepare pomace pulp, and pectinase is added to 1700U / mL for 2 hours. Finally, take the filtrate and add sucrose to 5g / L, adjust the pH value to 4.5, and sterilize it for later use; step (2) After preparing the normal temperature wine yeast into yeast seed liquid, inoculate according to the inoculation amount of 3mL / L, and stop culturing at 25°C for 3 days Ferment, centrifuge and get supernatant; step (3) add yeast powder 10g / L, sucrose 60g / L, ammonium sulfate 8g / L in the supernatant, adjust pH value 5.0, boil and sterilize; step (4) seed liquid Insert 100mL / L inoculum into the production fermentation culture medium, the thickness of the culture medium is 3cm, and culture at 30°C until the bacterial film ...

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Abstract

The invention discloses a method for producing bacterial cellulose by taking citrus pomace as a raw material, which belongs to the technical field of microbial fermentation and production. The method comprises the following steps: (1) pretreatment of the citrus pomace; (2) yeast fermentation of pretreatment liquid of the citrus pomace; (3) preparation of production and fermentation culture liquid; and (4) production and treatment of the bacterial cellulose, and the like. The citrus pomace is taken as the raw material, intermediate gluconacetobacter CIs26 CGMCC No.4663 which is autonomously selected and bred, the two-stage type fermentation process is adopted for producing the bacterial cellulose, the yield is as high as 13.5g / L, the production cycle is shortened from the conventional about half a month to 5-10 days, a product is semitransparent and good in flexibility, and the single situation of taking coconut milk as the raw material in the past is overcome. The method can be popularized and applied in bacterial cellulose production enterprises.

Description

technical field [0001] The invention relates to the technical field of microbial fermentation production, in particular to a method for producing bacterial cellulose by fermentation using citrus pomace as a main raw material. Background technique [0002] Cellulose is generally synthesized by green plants through photosynthesis, and mainly exists in various higher plants. It is the largest degradable natural polymer on earth and a renewable resource closely related to human life. It is widely used in food, feed, paper, etc. The technical field plays an important role. Modern studies have found that cellulose also exists in some lower plants, animals and bacteria. Among them, bacteria have the strongest ability to synthesize cellulose, the fastest speed, and the ability to produce on a large scale. In order to distinguish it from plant-derived cellulose, this microbial-derived cellulose is collectively referred to as bacterial cellulose (Bacterial Cellulose, BC). [0003] B...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12P19/04C12R1/02
Inventor 陆胜民杨颖夏其乐陈剑兵邢建荣张俊郑美瑜周锦云
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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