Bio-preparation method for production of high-purity xylo-oligosaccharide and coproduction of arabinose and xylose

A technology of xylo-oligosaccharides and arabinose, which is applied in the preparation of sugar derivatives, sugar derivatives, sugar derivatives, etc., can solve the problems of short service life of membrane, long production cycle, large energy consumption and water consumption, etc. The effect of improving product quality, low production cost and less pollution

Active Publication Date: 2012-09-12
SHANDONG LONGLIVE BIO TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The whole process has the characteristics of high energy consumption and water consumption (5-8 times of the treatment capacity), long production cycle and short membrane service life (12-18 months).

Method used

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  • Bio-preparation method for production of high-purity xylo-oligosaccharide and coproduction of arabinose and xylose
  • Bio-preparation method for production of high-purity xylo-oligosaccharide and coproduction of arabinose and xylose

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] (1) Wash 2.5 tons of corn cob powder (30 mesh) with 0.1% sulfuric acid at a solid-to-liquid ratio of 1:15 at 80°C for 1 hour under stirring conditions; separate the washing liquid after cleaning;

[0048] (2) After cleaning, mix ingredients according to the solid-liquid ratio of 1:7, stir evenly, and pretreat at 140°C for 2 hours;

[0049] (3) Add xylanase to the pretreated material at a dry matter amount of 30IU / g for enzymolysis. After enzymolysis for 5 hours, separate the slag-liquid, control the slag moisture below 75%, decolorize and concentrate the sugar solution, and then Activated carbon decolorization (decolorization temperature 80°C, carbon addition 0.5%, decolorization time 30min) followed by ion exchange (D301 and 001×7); after ion exchange, the conductivity is controlled to 100us / cm 2 , pH value 2.5 ~ 6.5; light transmittance above 70%;

[0050] (4) The mixed sugar liquid is concentrated to a concentration of 50%, and separated by three-phase chromatograp...

Embodiment 2

[0056] (1) Wash 2.5 tons of bran (30 mesh) with 0.1% hydrochloric acid at a solid-to-liquid ratio of 1:12 at 70°C for 2 hours under stirring conditions; separate the washing liquid after cleaning;

[0057] (2) After cleaning, mix according to the solid-to-liquid ratio of 1:6, stir evenly, and pretreat at 130°C for 2.5 hours;

[0058] (3) Add xylanase to the pretreated material at 20 IU / g of dry matter for enzymolysis. After 6 hours of enzymolysis, separate the slag-liquid, control the moisture content of the slag below 75%, decolorize and concentrate the sugar solution, and then Activated carbon decolorization (decolorization temperature 80°C, carbon addition 0.7%, decolorization time 30 min ) after ion exchange (D301 and 001*7); after ion exchange, control conductivity, 100us / cm 2 2.5~6.5; transmittance over 70%;

[0059] (4) The mixed sugar liquid is concentrated to a concentration of 50%, and separated by three-phase chromatography. The chromatographic conditions: the st...

Embodiment 3

[0065] (1) Take xylose mother liquor with similar components to AD liquor, with xylose content of 49%, arabinose content of 19%, and glucose content of 16%; the liquor is diluted to 23% and added with 0.5% yeast for fermentation to remove glucose, and decolorized by activated carbon , ion exchange, concentrated to 57%. Carry out two-phase chromatographic separation, chromatographic conditions: the stationary phase is calcium-type resin, the mobile phase is water; the instantaneous flow rate of step A is 0.412 m 3 / h, setting time 1130 S; step B instantaneous flow 0.412 m 3 / h, set time 108 S; step C instantaneous flow 0.121 m 3 / h, setting time 616 S; step D instantaneous flow rate 0.321 m 3 / h sets the time to 516 s. Through the adjustment of the Z column, the content of xylose phase reaches more than 80%, and the content of arabinose reaches more than 80%;

[0066] (7) Negative pressure crystallization of concentrated xylose solution with a concentration of 68%; centrifu...

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Abstract

The invention discloses a bio-preparation method for production of high-purity xylo-oligosaccharide and coproduction of arabinose and xylose. The method comprises the following steps of: washing hemicellulose-enriched biomass, performing size mixing pretreatment, adding xylanase, performing enzymolysis, and separating and purifying to obtain mixed liquid glucose; concentrating the mixed liquid glucose until the mass concentration is 50 to 70 percent, and performing chromatographic separation to sequentially obtain AD phase, BD phase, CD phase and BD phase, and concentrating the BD phase to obtain a high-purity xylo-oligosaccharide syrup liquid product; concentrating the CD phase, mixing with cellulosic materials, drying, and crushing to prepare fiber feed; performing yeast fermentation on the AD phase to remove glucose, removing impurities, purifying, concentrating and performing chromatographic separation to obtain the high-purity xylose liquid and arabinose liquid; concentrating the xylose liquid, crystallizing, separating and drying to obtain crystallized xylose; and concentrating arabinose liquid, crystallizing, separating and drying to obtain crystallized arabinose. The invention also provides a bio-preparation method for arabinose and xylose.

Description

[0001] technical field [0002] The invention relates to a biological preparation method of high-purity xylo-oligosaccharide co-production with arabinose and xylose, including the biological preparation method of high-purity xylo-oligosaccharide and its co-production of arabinose and xylose, and also includes the co-production of high Process for the preparation of pure arabinose and xylose. Background technique [0003] Arabinose, also known as pectin sugar, is often combined with other monosaccharides and exists in plant pulp, colloid, hemicellulose, pectic acid, coniferous tree heartwood, bacterial polysaccharides and certain glycosides in the form of heteropolysaccharides. It is rich in the hemicellulose and pectin of the cell walls of corn husks, corn cobs, rice, wheat and other grains, as well as plant cell walls such as sugar beets and apples. [0004] Xylooligosaccharides, also known as xylooligosaccharides, are functional polymeric sugars formed by combining 2 to 7...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/14C07H3/02C07H1/06
Inventor 肖林覃树林陈小刚李莹司红秀
Owner SHANDONG LONGLIVE BIO TECH CO LTD
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