19 results about "Azotobacter" patented technology

The invention discloses a biosynthetic bacterial cellulose-based solid electrolyte as well as a preparation method and application thereof, and belongs to the technical field of solid electrolytes. The solid electrolyte is prepared by taking modified bacterial cellulose prepared by fermenting acetobacter xylinum, rhizobium, pseudomonas, azospirillum, alcaligenes and other bacteria as a substrate, introducing a low-molecular-weight polymer to regulate and control the arrangement of cellulose molecular chains to serve as a conductive ion framework, and then compounding a conductive ion high-molecular-weight polymer and a lithium salt. According to the invention, cellulose molecular chains are modified by modified glucose, and a network structure is regulated and controlled by a low-molecular-weight polymer, so that the problems of small spacing, lack of ion coordination sites and weak ion transmission capability of pure cellulose molecular chains are effectively solved, the ionic conductivity of the prepared solid electrolyte can reach 10 <-4 > S cm <-1 >, the electrochemical window width reaches 5V, and the solid electrolyte has a good application prospect. The material can tolerate high critical current density, is suitable for various positive electrode materials, and is suitable for high-voltage and long-cycle-life energy storage devices.

This invention belongs to the field of bio-mineral fertilizer technology, specifically relating to a microbial liquid compound agent and bio-mineral fertilizer that combine coal gangue activation and heavy metal remediation. The microbial liquid compound agent comprises the following functional strains prepared through three-stage sequential inoculation and fermentation using electrochemical low-Hertz water: *Pseudomonas putida* (a remedial bacterium), *Azotobacter chrysogenum* (a nitrogen-fixing bacterium), *Azospirillum brasiliensis* (a growth-promoting bacterium), *Pseudomonas fluorescens* (a phosphate-solubilizing bacterium), *Bacillus subtilis* (a biocontrol bacterium), *Bacillus mucilaginosus* (a potassium-solubilizing bacterium), *Bacillus megaterium* (a silica-solubilizing bacterium), and *Trichoderma echinococcus* (a synergistic bacterium). The bio-mineral fertilizer is prepared using the aforementioned microbial liquid compound agent, with coal gangue powder as the main raw material. The scientific combination of different strains provided by this invention achieves efficient improvement of heavy metal-contaminated land and increased crop yields. Simultaneously, it transforms coal gangue into a valuable resource, solving the pollution problem caused by the large-scale accumulation of coal gangue, and possesses significant economic and social value.

The invention relates to the technical field of seed coating, in particular to a coating agent and a preparation method thereof. The coating agent comprises compound microorganisms, an antibacterial enhancer, a film-forming agent and a dispersing agent; the compound microorganisms comprise azospirillum, pseudomonas fluorescens, bacillus mucilaginosus and trichoderma harzianum; the antibacterial reinforcing agent is prepared from chitosan and epsilon-polylysine; the mass ratio of the compound microorganisms to the antibacterial enhancer is (1-3): (0.5-1). According to the coating agent and the preparation method thereof provided by the invention, the double effects of seed storage and preservation and growth promotion can be taken into account, and meanwhile, the environmental adaptability can be further improved.

The invention discloses a method for producing polyhydroxyalkanoate through microbial fermentation and application of the polyhydroxyalkanoate. The method for producing the polyhydroxyalkanoate through microbial fermentation comprises the following steps: optionally adding water into organic acid feed liquid for dilution, and then adding a complex microbial inoculant containing flavobacterium for aerobic fermentation to obtain PHA-rich fermentation thalli; the complex microbial inoculant comprises neosilurus meridionalis, nitrogen-fixing vibrio, dearomatization bacteria, flavobacterium and paracoccus; the organic acid feed liquid is obtained by carrying out acid production fermentation on a pretreated material derived from lignocellulose. According to the method, the lignocellulose is used as a raw material to replace a grain raw material to produce the organic acid under the condition of no enzymolysis. The microbial agent disclosed by the invention contains neosilurus meridionalis, dearomatization bacteria, flavobacterium, flavobacterium and paracoccus, the strains act together, the organic acid can be effectively utilized for further fermentation to generate polyhydroxyalkanoate, and the microbial agent has important economic and environmental benefits.

The invention belongs to the technical field of grassland restoration, and relates to a microbial ecological restoration method for a degenerated typical grassland, which comprises the following steps: selecting the degenerated typical grassland to be treated, ditching, applying a carrier growth-promoting microbial agent, and combining the carrier growth-promoting microbial agent with an arbuscular mycorrhizal fungus agent and / or an azotobacter agent according to the degeneration type. The carrier growth-promoting microbial agent comprises specific strains such as serratia rubra, enterobacter paraphora, cosinia cosinii, bacillus and halomonas. The carrier growth-promoting microbial agent is prepared from the specific strains such as serratia rubra, enterobacter paraphora, cosinia cosinii, bacillus and halomonas. The microbial inoculum is cultured and prepared by being loaded on an organic fertilizer carrier, and the application amount is adjusted according to different degeneration degrees. According to the method, vegetation recovery is remarkably promoted through the synergistic effect of three main microbial functional groups of soil, the biomass and coverage of plant communities are improved, soil nutrients and microbial activity are improved, and the method is suitable for various degeneration types such as grazing degeneration, cradling degeneration and desertification typical grassland; the method has the advantages of being good in remediation effect, environmentally friendly, high in sustainability and the like.

The present application relates to the microorganism source humic acid fertilizer for improving the relative abundance of azotobacter in the rhizosphere soil of oil bean, which is prepared by the following method: corn steep liquor is pre-decomposed by cellulase, alkaline protease and amylase, and then pre-decomposed by a fungus of Auricularia genus; the pre-decomposed corn steep liquor containing microbial fungal residues is diluted with water; the diluted corn steep liquor containing microbial fungal residues is reacted through three stages to produce artificial synthesized microorganism source humic acid; the artificial synthesized microorganism source humic acid liquid after reaction is dried to obtain the artificial synthesized microorganism source humic acid fertilizer; the artificial synthesized microorganism source humic acid fertilizer is diluted with water, and then applied to the root of oil bean at seedling stage, so that the relative abundance of azotobacter in the rhizosphere soil of oil bean reaches 5%-6%, and the length of the root of oil bean reaches 23-24 cm. The present application can improve the content of azotobacter (rhizosphere growth promoting bacteria) in the rhizosphere of oil bean, and improve the yield and quality of oil bean.

The present application relates to the technical field of biological pesticides, in particular to Paenibacillus sp. 18WLY, a bacterial agent containing the same and a fermentation liquor, and application of the Paenibacillus sp. 18WLY, the bacterial agent containing the same and the fermentation liquor in the prevention and treatment of wheat scab. The present application provides application of Paenibacillus sp. 18WLY, a bacterial agent containing the same and a fermentation liquor in the prevention and treatment of wheat scab. The bacteria can effectively prevent and treat wheat scab, and can be used as a green pesticide to reduce the use of chemical pesticides and improve the ecological environment.

This invention discloses a bio-fertilizer and soil improvement method for reducing continuous cropping obstacles in cucumbers. By weight, the bio-fertilizer comprises the following raw materials: 30-40 parts of well-rotted livestock and poultry manure, 20-30 parts of straw or rice husks, 1-2 parts of borax, 2-3 parts of urea, 2-4 parts of ammonium sulfate, 2-3 parts of zinc sulfate, 2-3 parts of magnesium sulfate, 1-2 parts of humic acid, and 1-2 parts of a microbial compound agent. The microbial compound agent comprises 4-6 parts of Bacillus megaterium, 2-3 parts of Bacillus polymyxa, 2-4 parts of Trichoderma echinococcus, 2-3 parts of Trichoderma viride, 3-5 parts of Pseudomonas fluorescens, 4-5 parts of Azotobacter chrysogenum, and 2-4 parts of Candida albicans. Using this bio-fertilizer and soil improvement method for reducing continuous cropping obstacles in cucumbers can significantly reduce the impact of continuous cropping obstacles, not only significantly reducing the incidence of cucumber wilt and root-knot nematodes and significantly promoting cucumber seedling growth, but also significantly increasing cucumber yield.

The invention discloses an alfalfa stress-resistant high-yield breeding method based on a microbiome regulation and control technology, and belongs to the technical field of microbial breeding. According to the breeding method, compound fertilizer doped with a microbial agent is applied in a seedling stage, a branching stage and a squaring stage respectively, and the compound fertilizer comprises a substrate and the microbial agent, the microbial agent is prepared from sinorhizobium meliloti, bacillus stearothermophilus, peste des petits ruminants, bacillus licheniformis, azotobacter brown, bacillus siamensis and bacillus amyloliquefaciens; the substrate comprises at least two of cow dung, pig manure, bean bran, corn straw and wood chips. According to the breeding method, the new microbial composition is prepared for preparing the compound fertilizer, the nutrition absorption capacity of the alfalfa can be improved through a microbial regulation function, and then the stress resistance and yield of the alfalfa are improved.

The invention relates to the technical field of organic solid waste resource utilization and microorganisms, and discloses a hermetia illucens sand staged matrix treatment method based on a special microbial agent and application of the hermetia illucens sand staged matrix treatment method. According to the method, hermetia illucens sand serves as a main treatment object, after the carbon nitrogen ratio and the water content are regulated and controlled, a special complex microbial inoculant composed of at least two microorganisms of bacillus, lactobacillus and azotobacter is inoculated, and under the condition that a continuous forced aeration device is not arranged, the hermetia illucens sand is subjected to aeration treatment for 20-30 min, and the hermetia illucens sand is obtained. The treatment process is divided into a microbial community construction stage and a low-disturbance stable transformation stage, and matrix treatment is completed through staged stirring regulation and control. According to the method, a microbial community establishment state is taken as a stage switching basis, high-frequency mechanical disturbance and continuous oxygen supply requirements are reduced, and conversion and humification of organic matters in the hermetia illucens sand and nitrogen stabilization are realized. And the obtained matrix product is stable in structure and relatively high in decomposition degree, and can be used for preparing a seedling substrate, an organic fertilizer or a soil conditioner.

This invention discloses the application of the protein OsDIF in regulating drought resistance and root colonization of *Azotobacter brasiliensis* in rice. The amino acid sequence of the protein OsDIF is shown in SEQ ID No. 1. Experiments have demonstrated that it improves homozygous mutant strains. Osdif‑1 The expression level of the protein OsDIF can improve drought resistance and root colonization of *Azotobacter brasiliensis*; while using a protein containing... OsDIF Recombinant Agrobacterium with a gene knockout vector transformed the rice variety Ishikari Shiro-mae, enabling it to... OsDIF Gene editing occurs when two homologous chromosomes... OsDIF When all genes mutate, the activity of the protein OsDIF is lost; this loss of OsDIF activity results in transgenic rice with reduced root colonization of *Azotomyces brasiliensis* and decreased drought resistance. Therefore, the protein OsDIF can regulate drought resistance and root colonization of *Azotomyces brasiliensis* in rice. This invention has significant application value.

The invention discloses recombinant rhizobium OEuspA with stress resistance, a plant salt-resistant bacterial agent and application of the plant salt-resistant bacterial agent, and belongs to the technical field of genetic engineering. According to the invention, a new uspA gene is identified from azotobacter stenoma ORS571 for the first time. And then carrying the uspA gene into the azotobacter stem nodule by using a plasmid vector, and constructing to obtain the recombinant rhizobium over-expressing the uspA gene. Compared with wild-type rhizobium, the stress resistance of the recombinant rhizobium for overexpressing the uspA gene, which is constructed by the invention, is remarkably improved. After the recombinant rhizobium over-expressing the uspA gene is symbiotic with sesbania eriocalyx, wheat and corn, the growth of plants under the salt stress condition can be promoted. The uspA gene disclosed by the invention can be used for constructing a recombinant rhizobium material with stronger stress resistance and growth promotion capability, and has potential and wide application prospects in the fields of genetic engineering transformation of rhizobium or other bacteria, the rhizobium microbial inoculum preparation industry, the bean planting industry in agricultural production and saline-alkali soil development.

This invention discloses a high-value-added fertilizer for fruits and vegetables containing fish protein, which is made from the following raw materials in parts by weight: 40-60 parts of compound microbial inoculant, 10-15 parts of activator, 80-120 parts of diatomaceous earth, 40-60 parts of decomposed microbial residue, 10-20 parts of potassium sulfate, and 10-30 parts of humic acid; the activator is composed of fish protein powder and onion extract in a mass ratio of 3:1; the compound microbial inoculant is composed of *Bacillus desertica*, *Azotobacter mollissima*, and *Pseudomonas brassicae*, with a volume ratio of 1:1:1. This fertilizer is based on microbial inoculants, using a combination of three specific advantageous strains for synergistic effects. It is also used in conjunction with an activator formulated from fish protein powder and onion extract in a specific ratio. This provides nutrients to plants while simultaneously enhancing microbial activity, enriching the soil microenvironment, and prolonging the microbial action time, resulting in significant yield increases, improved fruit and vegetable quality, and enhanced soil fertility.

The invention discloses a method for preparing PHA (polyhydroxyalkanoate) by using a complex microbial inoculant and application of the PHA. The method for preparing the PHA by using the complex microbial inoculant comprises the following steps: adding the complex microbial inoculant containing flavobacterium into a to-be-fermented liquid, and carrying out aerobic fermentation to obtain a PHA-rich fermented thallus; the liquid to be fermented contains organic acid feed liquid and optional water; the complex microbial inoculant disclosed by the invention comprises neosilurus meridionalis, nitrogen-fixing vibrio, dearomatization bacteria, flavobacterium and paracoccus; the organic acid feed liquid is obtained by performing acid production fermentation on raw materials of enzymatic hydrolysate containing pentose and hexose. According to the present invention, the composite bacterial agent with the high organic acid utilization rate is prepared, and the multiple organic acid fermentation liquids obtained from lignocellulose can be effectively utilized by using the composite bacterial agent so as to perform PHA synthesis, such that the large-scale production of PHA and the resource utilization of lignocellulose are easily achieved.

This invention relates to the field of ecological technology, specifically disclosing a soil matrix containing mud cake, its preparation method, and its application in ecological restoration. The soil matrix of this invention comprises a solid mixture and water. The solid mixture includes: 0.005%-0.01% nitrogen-fixing compound bacterial agent, 20%-45% organic compound slow-release material, 25%-40% mud cake, 28%-42% deep subsoil, 0.05%-0.1% cationic polyacrylamide, and 0.2%-0.4% vinyl acetate-ethylene carbonate copolymer; the nitrogen-fixing compound bacterial agent includes rhizobia, Azotobacter chrysophyllariae, Bacillus megaterium, and Azospirillum; the organic compound slow-release material includes organic fertilizer, wood flour, and fertilizer. This invention fully utilizes the large amount of mud cake generated in hydropower development and other projects, developing it into a substrate suitable for plant growth, especially providing a platform and carrier for plant growth on slopes, saving land resources, and possessing advantages such as high operability, low cost, and good ecological restoration effect.

The invention discloses an azotobacter composition and application thereof in enhancing sugarcane resistance, and belongs to the technical field of microorganisms. The method comprises the following steps: respectively carrying out fermentation culture on Azotobacter salinistris, Delftia tsuura and Vibrio natriegens, carrying out centrifugal resuspension, and mixing the obtained bacterial solutions, so as to prepare the composite bacterial agent capable of enhancing the resistance of the sugarcane. The method comprises the following steps: carrying out fermentation culture on Azotobacter salinistris, Delftia tsuura and Vibrio natriegens, and carrying out centrifugal resuspension on the obtained bacterial solutions so as to obtain the composite bacterial agent capable of enhancing the resistance of the sugarcane. When the complex microbial inoculant is applied to the sugarcane, the experimental result shows that the complex microbial inoculant can effectively enhance the resistance of the sugarcane to bacterial diseases and drought stress, and the three nitrogen-fixing bacteria have a remarkable synergistic interaction effect. The invention provides a novel microbial material and a treatment method for improving drought resistance and disease resistance of sugarcane in sugarcane planting, and the method is simple in step, low in cost, green and environment-friendly, and has outstanding application value and wide application prospect.

This invention provides a microbial composite chemical composition for improving saline-alkali land and its preparation method, belonging to the field of soil improvement technology. The composition for improving saline-alkali soil includes a composite microbial agent, polyglutamic acid, citric acid, sodium carboxymethyl cellulose, chitosan, and diatomaceous earth. The composite microbial agent is composed of *Bacillus licheniformis*, *Bacillus mucilaginosus*, *Azotobacter chrysophyte*, *Rhodopseudomonas palustris*, and purslane extract. This invention combines a composite microbial community with chemical conditioning components; the synergistic effect among these components effectively reduces the salinity and pH of saline-alkali soil and effectively increases soil organic matter content, achieving the effect of promoting growth and increasing yield.

The invention discloses a method for synergistically improving saline-alkali soil through a salt-tolerant microbial agent and fertilizer, and belongs to the technical field of microorganisms. Comprising the following steps: spraying a pH regulator after ploughing; the preparation method comprises the following steps: mixing and fermenting seed solutions of alkali-resistant marine rod-shaped bacteria, long-chain coralline actinomycetes and azospirillum molasses according to an equal proportion to prepare a composite salt-tolerant bacterial agent; preparing a solid functional fertilizer composed of a basic organic fertilizer, a slow-release inorganic fertilizer, a microbial protection growth promoting agent and the like; before sowing, solid fertilizer is spread, the fungicide is mixed with water to be sprayed on the surface of the fertilizer, and shallow ploughing and soil covering are carried out. According to the invention, a dominant bacterial community is constructed by three salt-tolerant growth-promoting bacteria with complementary functions, and the dominant bacterial community is matched with a specially-made solid fertilizer with microbial agent protection and nutrient slow release functions, so that the synergistic interaction of micro-ecological remodeling, nutrient slow release and soil structure improvement is realized. The pH and salt content of soil can be remarkably reduced, the cotton yield is increased, and the problems of difficulty in colonization and unstable effect of a microbial agent in a high-salt-alkali environment are solved.

This invention relates to the field of bio-organic fertilizer technology, and discloses a microencapsulated bacterial agent, a bio-organic fertilizer, its preparation method, and its uses. The microencapsulated bacterial agent provided by this invention includes a core particle and a coating layer; the core particle includes a composite bacterial agent, a carrier, and a binder; the composite bacterial agent includes Bacillus subtilis, Bacillus colloidis, and Azotobacter chroococcus; the carrier includes wheat bran and humic acid; the coating layer includes an inner layer, a middle layer, and an outer layer, which sequentially coat the surface of the core particle; the inner layer includes dextrin, molasses, and yeast extract powder; the middle layer includes ethyl cellulose and diatomaceous earth; and the outer layer includes chitosan, paraffin wax, and a plasticizer. The three coating layers work synergistically: the inner nutrient slow-release layer provides energy for the functional bacteria and maintains their activity; the middle physical isolation layer blocks humidity, compression, and harmful substances, stabilizing the microenvironment; and the outer soil triggering layer enables precise release, avoids premature inactivation, and significantly improves rhizosphere colonization efficiency.