247 results about "Azotobacter" patented technology

A composite biological agent with ternary microorganism for composting process of agricultural waste is composed of a composite biological agent I, a composite biological agent II, and a composite biological agent III, which is characterized in that the composite biological agent I is composed of bacillus, actinomycetes, microzyme and lactobacillus, the composite biological agent II is composed of trichoderma, ergillus, penicillium and trametes versicolor, and the composite biological agent III is composed of photosynthetic bacterium, azotobacter, phosphorus bacteria, and silicate bacterium. 30-60% composite biological agent I, 30-60% composite biological agent II, 10-20% composite biological agent III are mixed or applied to the process of composting process of agricultural waste in phases. Each component ratio and recruitment of biological agents can be both properly adjusted with different dung materials and dung purpose, thereby achieving purpose of using microorganism resources efficiently and raising fermentation efficiency.

The invention relates to a method for producing a high-efficiency composite microbial agent by using livestock liquid dung, and belongs to the technical field of environmental microbiology and agricultural science. The method comprises the following steps: diluting livestock liquid dung or livestock feces with water; after hydrolysis and acidification treatment, performing standing and settlement for desilting; mixing a supernatant obtained with a photosynthetic bacterial solution for anaerobic culture; using the mixed solution as a base liquid and stepwise adding lactobacilli, saccharomyces, bacillus thuringienesis and actinomycete into the base liquid in a proportion; and after anaerobic culture and aerobic treatment, finally filling the mixture into a biological fluidized bed for further treatment to obtain the high-efficiency composite microbial agent. The microbial agent is rich in the photosynthetic bacteria, the lactobacilli, the saccharomyces, the actinomycete, the bacillus thuringienesis, azotobacter, nitrobacteria, denitrifying bacteria and other beneficial organisms, and be widely used in agriculture, livestock breeding, fresh water aquaculture, organic sewage treatment, microbial deodorization, and other aspects. The technology is suitable to be used by various livestock farms (raisers) for liquid dung treatment.

The invention relates to a biotechnology dealing agricultural discard stem with an highly effective stem decomposing composite bacterial, which dissolves the problem of single species of applying microbiological bacterial, weak breaking up, low total degradability rate of cellulose, long developmental cycle. The composite bacterial comprises the following parts: yellow archespore wool bacterium leather bacterial, mushroom bacterial, haci trichoderma, green trichoderma, coninic trichoderma, aspergillus niger, bacillus subtilis, feed bacillus, bacillus megaterium, candida, distillery yeast, food-yeast, brown sphere azotobacter, thermoactinomyces, and so on. The composite bacterial can overall degrade lignin, cellulose, hemicellulose and other organic material in the stem, has a more than 80% degrading rate, a 10-15 days short cycle, keeps a long time for providing a solid bacteria.

The invention discloses a method to prepare leaven of fertilizer and application, which is characterized by the following: allocating raw material with mass ratio at 10-80 wt industrial residue of ferment, 5-30 wt straw, 5-30 wt cake meal, 10-30 wt bran or rice bran, 1.5-2 wt aquacare, 0.5-1 wt NaCl and or amylaceum, and 1-30 wt brown ball Azotobacter (Azotobacter chroococcum), 1-30 wt bacillus megaterium (Bacillus megaterium), 1-30 wt bacillus licheniformis (Bacillus licheniformis), 1-30 wt plant lactobacillus (Lactobacillus plantarum) and 1-30 wt 1-5 strain of saccharomycopsis fibuligera (Saccharomycopsis fibuligera); producing the product.

The invention relates to an agricultural compound biofertilizer, in particular to a compound biofertilizer capable of improving the physical and chemical properties of soil and improving the yield and quality of agricultural products and a preparation method thereof. The fertilizer contains the following materials in percentage by weight: 30-40% of organic substance, 15-25% of plant straw, 14-15% of mixed biological fermentation bacterium liquid and 30-31% of inorganic nutrient, wherein the biological bacteria used in the invention comprise Rhodopseudomonas palustris, microzyme, azotobacter, phosphorus-solubilizing Bacillus megaterium and silicate bacteria; the organic substance components are humic acid, worm excretion and bean cakes; and the inorganic nutrient comprises N, P and K nutrient. The compound biofertilizer can be applied as basal fertilizer or additional fertilizer, and is applicable to all crops. By using the compound biofertilizer, the yield increase amplitude can be 8-10%.

The invention relates to a fertilizer and a process for preparing the same, in particular to a green organic crop microorganism fertilizer. The microorganism fertilizer is characterized in that the microorganism fertilizer is prepared by the mix-fermentation of eight bacterial communities of photosynthetic bacteria, azotobacter, actinomycete, lactobacillus, yeast, root fixing bacteria, potassium bacteria and phosphorus bacteria, a plurality of beneficial microbial agents, coal gangue powder, lignite, straw carbon, humic acid, crop straw powder and excrement. Tests show that the microorganism fertilizer has obvious effect on the growth of corn, broomcorn, cereal, legume, paddy rice, fruits, flowers, pasture and the like, the production increasing rate is between 15 and 30 percent, and the microorganism fertilizer has obvious function on resisting diseases, lodging and insects, and can replace pesticide, fertilizer and hormone, avoid chemical pollution, improve soil fertility and product quality, and make the product become green or organic standards; and particularly, various crop straw and excrement are used as a carrier, thereby further lowering cost of the microorganism fertilizer and opening up a new path for the popularization and application of the microorganism fertilizer.

The invention relates to an alga microorganism fertilizer agent and a preparation method thereof and belongs to the technical field of aquiculture and the like. The alga microorganism fertilizer agent is fermented in such a way that monosodium glutamate waste liquid, alga paste, photosynthetic bacteria, actinomycetes, azotobacters and bacillus subtilis are compounded, and meanwhile, chitosan, chitosan oligosaccharide, microelements and molasses are added according to appropriate parts. The alga microorganism fertilizer agent is formed in such a way that the monosodium glutamate waste liquid is adopted for fermentation, and the raw materials preparation is simple; high-content compound bacteria are contained (the content of the bacillus subtilis is larger than three hundred million / mL, and the total content of the photosynthetic bacteria, the actinomycetes and the azotobacters is larger than one hundred million / mL) and the fertilizing effect is obvious; and the alga microorganism fertilizer agent also has a function of purifying water body, has a wide application range, and is suitable for sea-water and fresh-water cultivation.

The invention discloses a microbial fertilizer-plant synbiotic composition preparation and a method for preparing the synbiotic preparation. In the composition preparation, the plant synbiotic consists of probiotics, prebiotics and stabilizer; and bacillus forms zymotic fluid. The method for preparing the synbiotic preparation comprises the following steps: fermenting the probiotics; regulating bacteria amount; and preparing and homogenizing to obtain the product. Azotobacter, pralidoxime iodide and potassium bacteria in the preparation can increase soil nutrition, improve the fertilizer utilization rate and soil granule structures, promote the air permeability, water-retaining property and drainage, and overcome the defect of sunlight greenhouse continuous cropping; oligosaccharide prebiotics in the preparation can stimulate crops to improve resistance to pathogenic microorganisms, and promotes permanent planting and proliferation of the probiotics; the preparation can inhibit toxicity generated by bacteria, start self immunity system, eliminate diseases and remove evils, reduce bactericidal pesticide, has cold resistance, heat resistance, drought resistance, waterlogging resistance, saline and alkaline resistance, fertilizer damage resistance, chemical damage resistance and nutrition imbalance resistance, can promote plant root elongation and good development of capillary roots, and promote blooming and fruit yielding.

The invention discloses a microbial compound bacterial fertilizer and a preparation method thereof and belongs to the technical field of microbial bacterial fertilizers. The microbial compound bacterial fertilizer contains not less than 30% of organic matters, not less than 4% of nitrogen, not less than 4% of phosphorus and not less than 4% of potassium; furthermore, each gram of the bacterial fertilizer contains phosphate-solubilizing bacteria, potassium-releasing bacteria, nitrogen-fixing bacteria, alkaliphilic bacteria and halophiles, the number of each is 0.015-0.025 billion, and the number of thermophilic bacteria and thermophilic actinomycetes is 0.02-0.03 billion. The microbial compound bacterial fertilizer contains microbes for improving the soil structure, halophilic and alkaliphilic microbes and thermophilic microbes. The microbes for improving the soil structure contain abiogenous azotobacter and phosphate-solubilizing and potassium-releasing bacteria, and can enable the hardened soil (caused by the fertilizer) to become loose, increase the content of organic matters in the soil and improve the soil structure; the thermophilic bacteria and the thermophilic actinomycetes can be used for decomposing and composting straw, enabling the straw to become an agricultural fertilizer and increasing the soil fertility; halophilic and alkaliphilic bacteria can survive and proliferate in a saline-alkali soil environment, realize a special effect of improving the pH value of saline-alkali land, turn the saline-alkali land into treasure and enable the bacterial fertilizer to become a dual-purpose bacterial fertilizer for normal soil and saline-alkali soil.

This invention discloses a method for preparing microbial compound fertilizer chelated with trace elements for spray and drop irrigation. The method comprises: hydrolyzing raw material of protein wastes to obtain composite amino acids, adding trace elements to chelate composite amino acids, and combining with beneficial bacteria (azotobacter, phosphorus fertilizer bacteria, potassium bacteria, bacillus, etc.) to obtain the microbial compound fertilizer. The microbial compound fertilizer has obvious nitrogen fixation, phosphorus and potassium leaching, and growth promotion functions, and has high utility. The release speed is controllable, and the microbial compound fertilizer can improve and activate soil, and improve crop quality.

The invention discloses a biomass charcoal soil conditioner. The soil conditioner comprises the following raw materials by weight: 40% to 50% of biomass charcoal, 20% to 30% of a functional microbial agent, 10% to 15% of desulfurized gypsum, 5% to 10% of calcium nitrate, 5% to 10% of ground phosphate rock and 1% to 2% of amino acid, wherein the functional microbial agent contains any two selected from the group consisting of bacillus subtilis, bacillus mucilaginosus, bacillus amyloliquefaciens, azotobacter, rhizobia and bacillus megatherium. The biomass charcoal soil conditioner provided by the invention can increase the water-retention rate and air permeability of soil, improve the content of organic matters in the soil, and regulate the pH value of the soil; meanwhile, the adoption of biological means to improve microbial environment of the soil is beneficial for promoting crop growth and high-efficiency nutrient utilization, increases the yield of crops, and improves the quality of the crops. The biomass charcoal soil conditioner provided by the invention effectively utilizes wastes, can improve the soil and enhance soil fertility, and specifically has significant improvement effect on saline alkali land.

The invention relates to a novel microbial organic fertilizer with anti-actinomyces active compound bacteria and a preparation method thereof. The novel microbial organic fertilizer comprises a microbial inoculum and an organic fertilizer, wherein the microbial inoculum comprises colonies of Bacillus siliceous, Nocardia actinomyces, Bacillus subtilis, Bacillus thuringiensis, Brevibacillus laterosporus, azotobacter, Beauveria bassiana, Metarrhizium anisopliae, Trichoderma and Saccharomyces, and peptide proteins and rare-earth elements having unique effects on inhibiting wireworms and enhancing plant root system stress tolerancen; and the organic fertilizer comprises animal manure and plant fibers. The novel microbial organic fertilizer is prepared by fermenting the animal manure and plant fibers by using the microbial inoculum. The microbial organic fertilizer can quickly and effectively kill wireworm ova in soil, has strong effects on inhibiting and killing larvae and imagos, can continuously improve the growth environment of the plant root by using the growth of the microbial colonies and the stress tolerance of the peptide proteins, enables the plant to have strong roots, thick stems and green leaves, postpones the aging of the plant, and promotes prematurity and yield increase.

The invention relates to a multifunctional biological seed coating agent and a preparation method thereof. The multifunctional biological seed coating agent comprises auxiliary agents and beneficial microorganism, volume ratio thereof is 1:1-5, and effective bacterium content is not less than or equal to 10<8>cfu / ml. The auxiliary agent comprises one or various auxiliary agents in film former, dispersant, adhesion agent, protectant, nutritient, anti-freeze agent, preservative and colourant. The beneficial microorganism is selected from one or various rhizobium, azotobacter, pseudomonad, bacillus, K pneumoniae, Enterobacter cloacae and Raoulterlla planticola. The preparation method comprises the following steps: dissolving the polyvinyl alcohol and natural substance with water in a reaction kettle, mixing with the water dispersion liquid of the adhesion agent to prepare a film forming agent, and finally mixing and stirring mechanically with the beneficial microorganism fermentation liquor to obtain the finished product. The invention has the advantages of no residue, high efficiency, low toxicity, strong specificity, good film forming effect and convenient application, therefore, the multifunctional biological seed coating agent plays an significant role of preventing and controlling cotton seedling diseases as well as enhancing the cotton seedling growth under salt stress.

A recombinant microorganism is provided herein, in particular, a recombinant microorganism of the Azotobacteraceae family. The recombinant Azotobacter microorganism is capable of fixing atmospheric nitrogen continuously in the presence of oxygen and externally fixed nitrogen sources. The present invention further provides a process for production of the recombinant microorganism and a composition comprising the recombinant microorganism for use as biofertilizers and / or for use in the preparation of a fertilizer composition. The recombinant microorganism produced by this invention is an environmental friendly, highly beneficial microorganism.

The invention relates to the field of microbiology, and specifically relates to a composite microbial fertilizer and a preparation method thereof. The composite microbial fertilizer is composed of a composite microbial fermentation broth and an embedding medium, wherein a volume ratio of the composite microbial fermentation broth to the embedding medium are in a range of 1:5 to 1:4. The compositemicrobial fermentation broth is compounded of a co-culture comprising Candida utilis, lactic streptococci, lactobacillus acidophilus and bacillus subtilis, and a single nutrient solution comprising Bradyrhizobium japonicum, Azospirillum lipoferum, Rhodopseudomonas palustris, Bacillus megaterium, Bacillus mucilaginosus and Azotobacter chrococcum. In the invention, composite microbes processed by embedding can be stored stably for more than 60 days and a count of the composite microbes is always more than 2 billion per gram, thus the composite microbial fertilizer comprising the composite microbes has a long effective time and can improve a planting capacity of soil.

A plant growth promoting composition includes beneficial microbes and microbial activators. The beneficial microbes may include Bacillus spp., Azotobacter spp., Trichoderma spp., Saccharomyches spp., or combinations of these, the microtrial activators include enzymes, metabolites, carbohydrate yeast, humic material, staich, amino acids and micornutrients. The plant growth promoting composition may also include organic and inorganic fertilizers.

The invention provides a functional bactericide for preventing and treating root-knot nematode.The functional bactericide comprises an azotobacter bactericide, a biocontrol bacterium bactericide and a carrier, and the mass ratio of the azotobacter bactericide to the biocontrol bacterium bactericide to the carrier is (1-2.5):(1.5-5):1.The azotobacter bacterium bactericide contains nodule bacteria and azotobacter chroococcum, and the living bacterium count ratio of nodule bacteria to azotobacter chroococcum is (1-100):(1-200).The biocontrol bacterium bactericide contains paecilomyces lilacinus, verticillium chamydosporium, bacillus subtilis and bacillus cereus, and the living bacterium count ratio of paecilomyces lilacinus to verticillium chamydosporium to bacillus subtilis to bacillus cereus is (1-100):(1-100):(1-100):(1-100).The living bacteria count of the functional bactericide is larger than 0.2 billion / g.When applied to fertilizer, the functional bactericide can improve the disease preventing effect and the growth promoting effect of microbial fertilizer, guarantee nutrition supply of microbial fertilizer in the applying process, achieve the effects of preventing and treating root-knot nematode, forming healthy root nodules and promoting plant growth, increase the yield of crops and improve the quality of crops.

The invention discloses a micro-ecological flora of crop root system probiotics and an application thereof. The micro-ecological flora comprises a leading flora, a balance flora, a basic flora, a secondary-function flora and a special-function flora, wherein the leading flora comprises 25-35 parts of nitrogen-fixing bacteria by mass; the balance flora comprises 17-23 parts of phosphate solubilizing bacteria and 17-23 parts of potassium solubilizing bacteria by mass; the basic flora comprises 2-7 parts of yeast, 2-7 parts of rhizobia and 2-7 parts of photosynthetic bacteria by mass; the secondary-function flora comprises 2-7 parts of bacillus and 2-7 parts of actinomycetes by mass; the special-function flora comprises 2-4 parts of white rot fungi and 1-3 parts of pseudomonas. In the micro-ecological flora disclosed by the invention, the strain stability is good, functional microorganisms can be effectively planted in soil, and the functions of improving the crop yield and quality, improving the soil, detoxifying heavy metals and resisting diseases and pests can be realized.

The invention discloses a microbial coating agent for preparing a microbial compound fertilizer, which is prepared from 5-15% of curing agent, 35-75% of grease, 3-6% of surfactant and 10-55% of microbial culture, wherein the curing agent is a high-grade alkane mixture; the grease is animal fat, vegetable fat, petroleum extract or random-proportion mixture thereof; the surfactant is a conventional non-ionic surfactant or random-proportion mixture of the conventional non-ionic surfactants; and the microbial culture is a bacillus, azotobacter, actinomycete, microzyme or mycelial fungus culture, or a random-proportion mixture thereof. The coating agent disclosed by the invention is a non-aqueous microbial coating preparation, can realize high-concentration addition of active microbes into any granular fertilizer and implement coating, and has huge social meanings for development of environment-friendly fertilizers.

This invention discloses a microbial fertilizer containing rare earth elements. The microbial fertilizer is composed of active major strains, chitosan-type carrier, modified protective agent, neutralizer and rare earth element promoter. The major strains include Azotobacter, P / K-leaching bacteria (or silicate bacteria), and cellulose decomposing fungi. The chitosan-type carrier is suitable for both liquid and solid / freeze-dried microbial fertilizers, and can increase the survival rate of the major strains. The rare earth element promoter is chloride or nitrate of rare earth elements, and can increase the survival rate and fertilizing effect. Chitosan can rapidly degrade in natural soil conditions, thus bringing no pollution to soil.

The invention relates to a microbial fertilizer, in particular to a granular biological organic fertilizer. The preparation method is as follows: selecting azotobacter chrococcum, phosphate-solubilizing bacteria including bacteria megaterium and pseudomonas sp and a potassium-releasing bacterium circulans; carrying out amplification culture; adopting grass carbon for adsorption; carrying out airing and placing the products into capsules to prepare a granular bacterial agent; then sterilizing chicken manures and humic acid and grinding urea and monoammonium phosphate into powder with a grinder; adding water to the chicken manures, urea, monoammonium phosphate, potassium chloride, humic acid and zinc sulfate and stirring the mixture uniformly to prepare the granular organic fertilizer and finally mixing the prepared bacterial agent capsules with the organic fertilizer in a weight ratio of 1:10. The number of the active viable count in the fertilizer is ensured and the microbial fertilizer has prominent effect of self-decomposing the nutrients in the soil and especially effectively utilizes the phosphorus and potassium resources in the soil. After the fertilizer is applied to the corns for growth, the yield of the crops can be steady or increased, the beneficial microorganisms in the soil are increased and simultaneously the contents of available nitrogen, available phosphorus, available potassium and soil organic matters in the soil can be improved to some extent.

The invention belongs to the technical field of saline-alkali soil improvement and particularly relates to a saline-alkaline soil improving agent. The saline-alkali soil improving agent is characterized by comprising the following raw materials in parts by weight: 10-15 parts of flue gas desulfurization wastes, 20-30 parts of mixed grass meal, 3-8 parts of compound microorganisms, 5-15 parts of sawdust, 6-15 parts of sweet clover root powder and 10-15 parts of kieselguhr, wherein the mixed grass meal consists of sainfoin, symphytum officinale, coronilla varia and festuca arundinacea, and the compound microorganisms consist of nitrogen-fixing bacteria, Bacillus subtilis, bacillus licheniformis and bacillus mucilaginosus. The improving agent disclosed by the invention not only can greatly and effectively improve the current situations of saline-alkali soil, but also can utilize wastes and recycle resources and ecological vegetation, and is environment-friendly, safe and free of secondary pollution problem.

The invention discloses a chitin active calcium fertilizer and a preparation method thereof. The fertilizer is prepared from the following materials in parts by weight: 80-120 parts of sea shell waste, 8-12 parts of soybean pulp and 8-12 parts of fermentation liquid, wherein the fermentation liquid is prepared by uniformly stirring and mixing powder containing more than 200 million per gram of anaerobic compound bacteria, with water according to a ratio of 1:(8-10); the anaerobic compound bacteria are prepared by mixing bacterium powder obtained through respectively fermenting and culturing azotobacter, lactic acid bacteria, bacillus and actinomycetes. By adopting the fertilizer, not only is the problem of shell waste in coastal shell product cultivation effectively solved and are cultivation benefits of shell products improved, but also an organic fertilizer which has various advantages such as promoting the root system development, sterilizing powerfully, improving the soil, enhancing the immunity and improving the fertilizer efficiency can be produced.

The invention discloses a Nodule Azotobacter strain BXYD3 and the application. The strain is obtained by a way that the Rhizobia strains in the soil are respectively captured through different soybean varieties; the Rhizobia strains are separated and purified in fresh root nodules; part 16S rDNA of the strain is sequenced; the phylogenetic analysis determines that the strain is a novel Bradyrhizobium strain, which is named as Huagu 3. The Nodule Azotobacter strain BXYD3 of the invention has the characteristics of acid and aluminum tolerance and high level of nodules, which are proved by laboratory pot culture and field experiment. The inoculation of the Rhizobia strain can improve the yield of soybean more than 20 percent to 30 percent. The invention suits for the acid soil area in South China, which can be used as one of the measures for the conventional cultivation of soybean.

The invention provides a biological organic fertilizer, which mainly comprises peat containing adsorbed liquid microbial agent, livestock-poultry manure fermented through liquid microbial agent, as well as humic acid, wherein the mixture ratio is that the peat with adsorbed microbial agent accounts for 10 to 20 percent; the fermented livestock-poultry manure accounts for 35 to 45 percent; and the humic acid accounts for 40 to 55 percent. A production method of the biological organic fertilizer mainly comprises the steps of producing the peat with adsorbed microbial agent and the fermented livestock-poultry manure, well mixing the peat with adsorbed microbial agent, the fermented livestock-poultry manure and the humic acid pro rate, detecting and packaging products. The biological organic fertilizer has the advantages that the fertilizer contains various nutrient elements, azotobacter, phosphorus / potassium dissolving bacteria and the like which are necessary for crop growth, is free from public nuisance and pollution, can improve soil, increase land fertility and strengthen the stress resistance of crops, and is low in cost, high in benefit, convenient to use and applicable to various types of soil and crops. The production process of the biological organic fertilizer is simple, reasonable and short in the fermentation time of livestock-poultry manure, can effectively reduce harmful substances, preserves effective constituents, and reaches the standard of production means for producing green food.

The invention relates to a biological fertilizer production technique for sufficiently utilizing arable layer nitrogen in order to eliminate nitrogen pollution, which relates to a production technique of a biological fertilizer, in particular to a production method of a biological fertilizer compounded with nitrobacteria, azotobacter and the like. The technique adopts the following technical processes: (1) using the method known by professionals in the industry to screen bacteria such as azotobacter, photosynthetic bacteria, algae, legume bacteria, nitrosobacteria, nitrobacteria, trichoderma, silicate bacteria, bacilli and denitrifying bacteriap; (2) carrying out experiments on the effects of the screened strains; (3) using the known method to respectively propagate each strain step by step according to the characteristics of the strain; (4) selecting different carrier materials for adsorption or drying according to the characteristics and purposes of the strains; (5) finally carrying out inspection and packaging. The invention is applicable to large-area mechanical spreading operation as well as manual spreading operation on crofts.

The invention relates to the field of micro-organisme fungicide and soil amelioration, and particularly discloses a method for accelerating soil carbon in the corn-wheat crop rotation system. The method is as follows: in the corn-wheat crop rotation system, after having harvested corn and wheat, shattering and returning to the field the corn straw and wheat straw, spraying the micro-organisme fungicide agent that is capable of accelerating the fermentation and decomposition of straw on the straw surface, and scattering organic fertilizer. After sowing corn and wheat, applying soil conditioner that contains azotobacter, combined with compound fertilizer, and spraying liquid amino acid fertilizer for plant during the growth phase of corn and wheat. The present method can reduce as much as possible the destabilization of soil and accelerate the decomposition of straw using biological bacterium, to guarantee the activity and diversity of micro-organisme in arable soil. The present method uses soil conditioner to regulate soil structure, thereby strengthening soil fertility and accelerating the accumulation of soil carbon.

A recombinant microorganism is provided herein, in particular, a recombinant microorganism of the Azotobacteraceae family. The recombinant Azotobacter microorganism is capable of fixing atmospheric nitrogen continuously in the presence of oxygen and externally fixed nitrogen sources. The present invention further provides a process for production of the recombinant microorganism and a composition comprising the recombinant microorganism for use as biofertilizers and / or for use in the preparation of a fertilizer composition. The recombinant microorganism produced by this invention is an environmental friendly, highly beneficial microorganism.

The invention discloses a method for improving the output of crude oil by utilizing a synergistic effect of combined bacteria liquid containing phosphate-solubilizing microorganisms and nitrogen-fixing bacteria. The method comprises the steps: injecting diluent of a compound microorganism bacterium agent prepared from pseudomonas fermentation liquor with a phosphate-solubilizing function, azotobacter fermentation liquor and combined bacteria liquid with a petroleum hydrocarbon degrading function into an oil pool via a water injection well or injecting through a production well, and extracting oil to obtain the crude oil. The compound microorganism bacterium agent is prepared from the pseudomonas fermentation liquor, the azotobacter fermentation liquor and the combined bacteria liquid with the petroleum hydrocarbon degrading function according to a ratio of 1 to 1.2 to 1.6-8; the combined bacteria liquid with the petroleum hydrocarbon degrading function contains bacillus, acinetobacter and xanthomonas; and the three bacteria are independently cultivated into single bacteria liquid of 108-1010cfu / ml, and then mixed iso-volumetrically, so that the combined bacteria liquid with the petroleum hydrocarbon degrading function is obtained. According to the invention, the method is simple in construction, short in time, low in cost, large in yield rate and high in oil extraction productivity.

The invention relates to a nano-biological organic-inorganic compound fertilizer, which comprises the compositions by weight: 40-48 percent of biological organic fertilizer, 11-18 percent of nitrogenous fertilizer, 16-20 percent of phosphate fertilizer, 8-12 percent of potassium fertilizer, 10-15 percent of bentonite and 0.1-0.15 percent of nanometer type granulating adhesive. Sauce pomace is fermented through azotobacter, potassium bacteria and phosphorus bacteria, thus obtaining the biological organic fertilizer. Effective components in the fertilizer are counted by weight: organic matter is more than or equal to 38 percent, nitrogen plus phosphor plus potassium is more than or equal to 15 percent and number of beneficial microorganisms is more than or equal to 100 million of every gram fertilizer. The invention also relates to a preparation method of the nano-biological organic-inorganic compound fertilizer. The fertilizer of the invention changes waste into valuables, has high utilization ratio of the fertilizer, improves soil structure, adjusts the environment of microorganism, strengthens stress resistance of crops and has good effect of disease and pest resistance; the quality of the invention is comparable to farmyard manure and the invention is an ideal fertilizer for planting uncontaminated vegetables.