91 results about "Guluronic acid" patented technology

The invention relates to an optionally foamed sealing composition for preformed seals which can swell when treated with water. The invention also relates to a method for the production thereof out of natural rubber and / or elastomers with a matrix comprised of natural rubber / elastomer components and particle-shaped water absorbing material stored therein. The water absorbing material is a combination of (A) polysaccharide(s) selected from cellulose, starch, starch derivatives removed from grafted starch, amylose, amylopectin, dextran, pectin, inulin, chitin, xanthan, alginic acid, alginates, carrageenan, pustulan, callose, laminarin, guluronic acid, pullulan, lichenin or mixtures of the same with (B) a highly water absorbent synthetic polymer selected from polymers based on (meth)acrylate, poly(meth)acrylic acid and the salts thereof, polyacrylamide, polyalcohols or copolymers of said synthetic polymers. The invention also relates to additional cross-linking and processing auxiliary agents and to property improving agents. It is possible to securely seal superstructures, substructures, tunnels and canals with the assistance of the inventive sealing compositions.

The invention discloses a sodium alginate oligosaccharide coated slow-release fertilizer. Coated materials of the slow-release fertilizer contain sodium alginate oligosaccharide and an auxiliary coated material, wherein sodium alginate oligosaccharide is composed of two structural units, namely a-L-mannuronic acid and b-D-guluronic acid. The two structural units are linked in three ways by 1,4-glucosidic bond, so as to form a unbranched linear segmented copolymer. The coated slow-release fertilizer is mainly applied in commercial crops, fruit and vegetable crops, grain crops, economic forest, flowers and plants, traditional Chinese medicinal materials, lawn and the like. By introducing organism-derived sodium alginate oligosaccharide into the coated materials of the slow-release fertilizer, nutritive elements required by plant growth and development can be supplemented by a slow-release approach, and the plant growth promoting effect of sodium alginate oligosaccharide also can be performed so as to finally achieve the yield-increasing effect. In addition, a medicament prepared by using sodium alginate oligosaccharide as the main coated material composition is safe and nontoxic and has advantages of low cost, small application amount and the like.

The invention discloses a leaf fertilizer composition comprising sodium alginate oligosaccharide, which is characterized in that the composition comprises sodium alginate oligosaccharide, amino acid and microelements that can promote plant growth significantly, wherein sodium alginate oligosaccharide is also known as sodium alginate, sea-tangle glue and algin, is natural polysaccharide carbohydrate extracted from sea tangle, and is a copolymer comprising a-L-mannuronic acid (M), b-D-guluronic acid (G) connected with a-L-mannuronic acid (M) by 1,4-glycosidic bond, and different GGGMMM fragments; a molecular formula is (C6H7O6Na)n; the degree of polymerization is 2-10; the uronic acid constitution M / G is equal to 7:3; the uronic acid content is greater than 90%, and is 2-3% of the total content; the content of a microelement leaf fertilizer is 2-20%; and the microelement leaf fertilizer comprises at least two of sulfur, boron, ferrum, manganese, copper, zinc and molybdenum. The composition is mainly applicable to industrial crops, fruit and vegetable crops, grain crops, economic trees, flowers, lawns and the like.

Hemostatic devices for promoting blood clotting can include a substrate (e.g., gauze, textile, sponge, sponge matrix, one or more fibers, etc.), a hemostatic material disposed thereon such as kaolin clay, and a binder material such as crosslinked calcium alginate with a high guluronate monomer molar percentage disposed on the substrate to substantially retain the hemostatic material material. When the device is used to treat a bleeding wound, at least a portion of the clay material comes into contact with blood to accelerate clotting. Moreover, when exposed to blood, the binder has low solubility and retains a majority of the clay material on the gauze. A bandage that can be applied to a bleeding wound to promote blood clotting includes a flexible substrate and a gauze substrate mounted thereon.

The present invention provides edible compositions comprising a source of non-solubilised divalent metal ions and from 0.1 to 6% wt of an alginate having an L-guluronic acid content of at least 60% of the total uronic acid units in the alginate. The compositions of the invention have good satiety effects and are beneficial for use in weight control plans. The edible compositions may be a food compositions intended for use in a weight loss or weight control plan such as a meal replacer food product.

The invention relates to a method for extracting active polysaccharides from brown algae. The method comprises the following specific steps of: putting a cleaned, dried and smashed alga raw material into an extraction container, and adding an organic acid solution or organic-inorganic mixed acid solution; heating a stirred and wetted material to 50-150 DEG C to realize partial degradation of highpolymer polysaccharide substances of brown algae; fully washing with an organic solvent till the acid solution in the material is discharged completely; adding 5-8 times of volume of water for extracting, concentrating an extracting solution, regulating the PH value to neutral, and gradually depositing in alcohol to obtain align, fucoidin and laminaran which are rich in mannuronic acid fragments (rich in M) respectively; and pretreating brown alga raw material residues with water-extracted organic acid according to an alkali digestion process to obtain align which is rich in guluronic acid fragments (rich in G). By adopting the method, the defects of high water consumption, severe pollution and the like existing in the conventional align production process are overcome; and the method has the advantages of saving in energy, reduction in emission, high product yield, small extraction solvent dosage, and the like.

The present invention discloses an alginic acid and / or its salts with low molecular weight, wherein the weight average molecular weight of the alginic acid is from about 700 to about 4500 Daltons, and the molar ratio of guluronic acid to mannuronic acid in the alginic acid is from about 0.6 to about 19. The present invention also discloses the preparative method of making the alginic acid and / or its salts thereof, and the use of them for treating hypertension, chronic renal failure and postprandial hyperglycemia induced by glycosidase. The present invention further discloses pharmaceutical compositions and foods containing the alginic acid with low molecular weight and / or salts thereof as active component.

The invention discloses an alginate lyase (Alg509) derived from marine bacteria and a gene thereof, and also disclosed a method for recombinant expression and preparation of the alginate lyase. According to the method, the alg509 gene is cloned into an E. coli expression vector, and the vector is transformed into an E. coli host strain to obtain a recombinant engineering strain which can heterologously express the enzyme. The alginate lyase Alg509 disclosed in the invention has high enzyme activity, the specific enzyme activity can reach up to 48000 U / mg and above, the optimum reaction pH is 10, the optimum reaction temperature is 55 DEG C, and the enzyme activity has no dependence on various metal ions. The enzyme is active to sodium alginate, poly-guluronic acid (polyG) and ploymannuronic acid (polyM), and can completely degrade sodium alginate to produce alginate oligomers such as alginate disaccharide, alginate trisaccharide, alginate tetrasccharide, etc. The enzyme exhibits strongbasophilia, has certain tolerance to high pH, has certain potential of industrial applications, and can be widely applied in the fields of agriculture, food, feed additive, medicine and the like.

This invention relates to an extraction process of brown algae polysaccharides in a field of pharmaceutical chemistry. This invention particularly discloses a process of extracting brown algae polysaccharides based on a microwave chemistry method and brown algae polysaccharides obtained by said process. The process of the invention comprises: 1) putting pulverized brown algae powder into a microwave reaction chamber, adding acid solution to conduct reaction; optionally concentrating the mixer, and then washing with organic solvent to remove excess acid; conducting grading alcohol precipitation after water extract to obtain mannuronic acid rich fragment (M rich) algin, fucoidan and / or laminaran respectively; and adding an alkali solution to the brown algae residue to conduct alkaline digestion, filtering the residue off, adjusting pH of the filtrate to neutral, conducting alcohol precipitation to obtain guluronic acid rich fragment (G rich) algin precipitates. The present invention has significant advantages like fast processing rate, high yield of polysaccharides, strong controllable polysaccharide degradation, using less organic acid and efficient recovery, small water consumption, low power consumption, etc., the active polysaccharides has high yield and content, better water-soluble, and good biological activities.

A preparation method for alginate oligosaccharides includes following steps: adding the water to the alginic acid or metal salt or the produced high mannuronic acid, high guluronic acid section to dissolve or disperse in the water, adding it to the oxidant solvent in a radiation condition, heating the solution to 60-80 DEG C for 4-50 minutes, then cooling the solution, concentrating the solution by nanofiltration film, drying the concentrated liquid by spray. The invention improves the product quality by preventing the product over-oxidation by the radiation assistance in a lower temperature; effectively reduces the dosage of the oxidant by controlling the product degree of polymerization distribution in a low reaction temperature; avoids using organic solvent, reduces the product costs and saves the cost by nanofiltration film separation technique and spary drying technique which can be achieve the industry mass production. Therefore, the method has a practical application value.

The invention discloses a method for in-vitro amplification of tumor stem cells. The method comprises the following steps of carrying out in-vitro three-dimensional culture on tumor cells by an alginate gel support, and adjusting and controlling the rigidity of the alginate gel support by a change of support preparation parameters such as alginate molecular weight, a ratio (GM) of guluronic acid content to mannuronic acid content, a calcium ion concentration and calcification time, so that hardness of an in-vitro matrix in different tumor tissue microenvironments is simulated and in-vitro amplification of tumor stem cells in different tissue is realized. The method can be operated simply, has a low cost and controllable performances, can be industrialized and is a good method for in-vitro amplification on tumor stem cells having different sources.

The invention relates to facultative incision type recombinant alginate lyase rAly-1 as well as a coding gene and application thereof. An amino acid sequence of the facultative incision type recombinant alginate lyase rAly-1 is as shown by SEQ ID NO. 2; a nucleotide sequence of the coding gene of the facultative incision type recombinant alginate lyase rAly-1 is as shown by SEQ ID NO. 1. According to the facultative incision type recombinant alginate lyase rAly-1 as well as the coding gene and the application thereof, the facultative incision type recombinant alginate lyase rAly-1 is obtained from a genome of flammeovirga yaeyamensis MY04 for the first time; not only can the lyase be used for degrading a guluronic acid segment from alginate, but also can be used for degrading a mannuronic acid segment. The lyase can be used for thoroughly degrading alginate polysaccharide in any M / G ratio, and efficiently preparing alginate oligosaccharide with a lower polymerization degree.

Process for preparing a modified alginate polymers are disclosed. The processes comprise the steps of covalently attaching a modifying moiety to one or more unmodified monomeric subunits of an alginate polymer; and changing one or more unmodified mannuronic (M) monomeric subunits of the alginate polymer to one or more unmodified guluronic (G) monomeric sub-units by an enzymatic epimerization reaction performed in any order. Processes for preparing alginate gels, fiber, and compositions are also disclosed. Modified alginates in which only M monomeric subunits are modified, and alginate gels, fibers and compositions comprising the same, are disclosed.

The present invention provides methods for producing a product of one or more enzymatic pathways. The pathways used in the methods of the invention involve one or more conversion steps such as, for example, an enzymatic conversion of guluronic acid into D-glucarate (Step 7); an enzymatic conversion of 5-ketogluconate (5-KGA) into L-Iduronic acid (Step 15); an enzymatic conversion of L-Iduronic acid into Idaric acid Step 7b); and an enzymatic conversion of 5-ketocluconate into 4,6-dihydroxy 2,5-diketo hexanoate (2,5-DDH) (Step 16). In some embodiments the methods of the invention produce 2,5-furandicarboxylic acid (FDCA) as a product. The methods include both enzymatic and chemical conversions as steps. Various pathways are also provided for converting glucose into 5-dehdyro-4-deoxy-glucarate (DDG), and for converting glucose into 2,5-furandicarboxylic acid (FDCA). The methods also involve the use of engineered enzymes that perform reactions with high specificity and efficiency. Additional products that can be produce include metabolic products such as, but not limited to, guluronic acid, L-iduronic acid, idaric acid, glucaric acid. Any of the products can be produced using glucose as a substrate or using any intermediate in any of the methods or pathways of the invention.

The invention discloses a water-locking antibacterial material for diapers and a preparation method of the water-locking antibacterial material, and belongs to the field of baby products. The water-locking antibacterial material for diapers comprises the following raw materials: polyether sulfone resin, corn starch, polylactic acid, chitosan, alpha-L-guluronic acid, beta-D-mannuronic acid, gelatin, lignin, phtheirospermum japonicum, sodium hydroxide, cholic acid, polyaspartamide, herba andrographis, radix cordylines fruticosae, fructus forsythiae, rhizoma anemarrhenae, rhizoma polygoni cuspidati, silk fibroin, acrylic acid, sodium alginate gel, hydroxyethyl acrylate, glycerol, polyacrylamide, potassium persulfate, sodium sulfite, N, N-methylene bisacrylamide, modified antibacterial additives, and water-locking and reinforcing materials. The invention discloses specific steps of a preparation method of the water-locking antibacterial material for diapers. The water-locking antibacterialmaterial for diapers provided by the invention has excellent antibacterial and water-locking properties.

The invention discloses a quality breeding method for sargassum fusiforme. The method comprises the steps that 1, ripe sargassum fusiforme alga bodies are selected to serve as main weeds, the quality ingredients of the main weeds are determined, and the main weeds of which the quality ingredients are in accordance with the following requirements are selected for seedling culturing, wherein the polysaccharide content, by dry weight, of the main weeds is larger than or equal to 40%, and the proportion sum of fucose, guluronic acid and mannuronic acid is larger than or equal to 80% by dry weight of the polysaccharide content of the sargassum fusiforme; 2, after the main weeds which are in accordance with the quality requirements are collected, lower alga bodies of the main weeds are removed, upper and middle alga bodies are retained, a female plant and a male plant are combined into a pair and put in a seedling culturing pool to be cultured for five to eight days, sperms and eggs are released and fertilized to form young embryos, and the young embryos are collected. According to the quality breeding method for the sargassum fusiforme, the breeding effect of the sargassum fusiforme can be significantly improved, the sargassum fusiforme obtained through the breeding method is more uniform in quality and good in breeding effect, the main weeds can be randomly selected for breeding, and the breeding quality of the main weeds is stable.

The invention belongs to the technical field of a lithium ion battery and particularly relates to a negative electrode of the lithium ion battery. The negative electrode comprises a negative electrode current collector and a negative electrode active substance layer coated on the negative electrode current collector; the negative electrode active substance layer comprises a negative electrode active substance, a conductive agent and a binder; and the binder is alginate, wherein the alginate contains two stereoisomers, i.e. L-guluronic acid (G segment) and D-mannuronic acid (M segment), the ratio of G / M is less than or equal to 1.5, and the content of calcium impurities contained in the alginate is less than or equal to 0.3%. Compared with the prior art, the negative electrode has the advantages that after the alginate is adopted as the binder of the negative electrode of the lithium ion battery, the volume change of the lithium ion battery during the process of lithium de-intercalation can be inhibited, and the binding effect between the negative electrode active substance layer and the binder of the negative electrode is improved, so that the volume expansion coefficient of the battery during the cyclic process is lowered, and the properties of low temperature and multiplying factor of the lithium ion battery are improved. Moreover, the invention further discloses the lithium ion battery containing the negative electrode.

The present invention is directed to an ophthalmic composition comprising alginate having a minimum of about 0% and a maximum of about 50% guluronic units bound to an adjacent guluronic unit as a percentage of the total number of monomeric units in the alginate. The composition further includes a cationic antimicrobial agent. The alginate set forth above forms less deactivating complexes with cationic antimicrobial agent, and thus, the composition has improved preservative efficacy.

The invention discloses a broad-spectrum flower and fruit protector for plants and application of the broad-spectrum flower and fruit protector. An active component of the broad-spectrum flower and fruit protector adopts alginic acid oligosaccharide or an alginic acid oligosaccharide derivative; the alginic acid oligosaccharide is oligosaccharide obtained by connecting mannuronic acid with guluronic acid through a beta-1,4-glucosidic bond, and the molecular weight of the alginic acid oligosaccharide is 300 to 10,000 Da; the alginic acid oligosaccharide derivative comprises an alginic acid oligosaccharide sulfuric acid derivative and an alginic acid oligosaccharide phosphorus-containing derivative. The broad-spectrum flower and fruit protector is applied to increasing the flower protection and fruit setting rate in growing and developing processes of vegetables and fruit trees; the broad-spectrum flower and fruit protector is applied to the vegetables and the fruit trees. By the use of the broad-spectrum flower and fruit protector, the fruit setting rate can be increased by 40 to 80 percent, and particularly, the fruit setting rate of the vegetables is increased by 30 to 80 percent. A drug with the alginic acid oligosaccharide serving as a main component has the advantages of safety, nontoxicity, low cost, small application amount, environment friendliness, durable effect and the like. The broad-spectrum flower and fruit protector for the plants is used for carrying out application, seed soaking, root dipping, root irrigation and the like on crops such as the fruit trees, so that the flower and fruit protection capacity of the crops can be improved, and the broad-spectrum flower and fruit protector has an outstanding effect on increase of the crop yield and improvement of the quality.

The invention provides application of guluronic acid oligomers in preparing medicines, health products or dietary supplements for preventing and treating alcoholic liver injury. Guluronic acid oligomers with different molecular weights, which are obtained by degrading polyguluronic acid graded and purified from algin, can lower the hepatosomatic index increase caused by alcoholic injury, resist triglyceride and cholesterol increase caused by alcohol, effectively lower glutamic-pyruvic transaminase and glutamic oxaloacetic transaminase increase in serum caused by alcohol, enhance the total oxidation resistance, glutathione level and superoxide dismutase activity in the serum, inhibit the generation of malonaldehyde in the liver caused by alcohol, lower the mouse drunkenness rate, prolong the mouse drunkenness time, and shorten the sober-up time, thereby implementing the protective action and hangover alleviating function on alcoholic hepatic injury. The products provided by the invention are derived from marine natural products, have the advantages of abundant resources, high industrialization tendency, high safety, high effectiveness and the like, and have wide development and application prospects in the aspects of prevention and treatment of alcoholic hepatic injury, hangover alleviation and liver protection.

The invention belongs to the field of marine drugs, and in particular relates to an application of oligoguluronates in preparation of drugs for prevention and treatment of liver damage and various hepatitis, liver fibrosis or cirrhosis. The oligoguluronates are used in preparation of the drugs for protection of livers and treatment of various hepatitis, liver fibrosis or cirrhosis; the oligoguluronates adopt algin as a raw material, and oligoguluronic acids with different molecular weights are obtained through a chemical degradation method or a physical degradation or a combination of the two degradation methods; the oligoguluronic acids are neutralized to prepare lithium salts, sodium salts, potassium salts, calcium salts or magnesium salts of the oligoguluronic acids with different molecular weights, wherein a molecular skeleton is a linear oligosaccharide compound formed by connecting L-guluronic acid (G) through alpha-(1,4)-glycosidic bonds. The products are derived from the marine natural product, have many advantages of abundant resources, easy industrialization, safety, effectivity and the like, and have broad development and application prospects in prevention and treatment of liver damage and various hepatitis, liver fibrosis or cirrhosis.

The invention discloses a method for rapidly determining an oligoguluronic acid employing a UPLC-Q-TOF / MS (ultra-high performance liquid chromatography-quadrupole rods tandem time-of-flight / mass spectrometry) technology and belongs to the technical field of UPLC-Q-TOF / MS detection. The oligoguluronic acid is rapidly determined by the following steps: preparing a standard substance, pretreating a sample, and building a chromatographic condition, a mass spectrum condition and a qualitative and quantitative method for the oligoguluronic acid. The oligoguluronic acid is rapidly and accurately detected; and the characteristics of the oligoguluronic acid in parent ions and fragment ions in the mass spectrum are given, so that accurately qualitative and quantitative determination of a guluronic acid from monosaccharide to tetrasccharide in the sample can be realized; and meanwhile, the unknown oligoguluronic acid which is possibly contained in the sample can be speculated and analyzed according to the mass spectrum information.

The invention relates to a corrosion inhibitor for a steel neutral medium, particularly relating to a green sodium alginate corrosion inhibitor for a carbon steel neutral medium as well as an application thereof. The corrosion inhibitor is sodium alginate of which the chemical general formula is (C6H7NaO6)n and the average molecular weight (Mw) is 20000-100000. The sodium alginate corrosion inhibitor disclosed by the invention is a natural product extracted from brown algae, and is widely applied to food industry. The corrosion inhibitor is a copolymer formed by connecting alpha-L-mannuronic acid (M units) with beta-D-guluronic acid through 1,4-glucosidic bonds and composed of different GGGMMM fragments. The alpha-L-mannuronic acid unit contains a large amount of carboxyl and can be coordinated with metals. Compared with the current commonly-used organic corrosion inhibitor, the corrosion inhibitor disclosed by the invention is non-toxic and harmless, thereby having no environmental problem after use. In addition, the corrosion inhibitor is non-toxic and harmless to environment and living beings, thus conforming to development of green corrosion inhibitors.

The invention belongs to the field of marine drugs, and concretely relates to an application of oligomeric guluronic acid salts in the preparation of Parkinson's disease prevention and treatment drugs or products. The oligomeric guluronic acid salts can be used for preparing Parkinson's disease prevention and treatment drugs or products, and are obtained through a step of carrying out a chemical degradation process or a physical degradation process on algin as a raw material to obtain oligomeric guluronic acid with different molecular weights, and neutralizing the oligomeric guluronic acid with different molecular weights to prepare lithium salt, sodium salt, calcium salt or magnesium salt of oligomeric guluronic acid with different molecular weights, wherein the molecular skeleton is a linear oligosaccharide compound formed by bonding L-guluronic acid (G) through an alpha-(1,4)-glycosidic bond. Experiments in the invention prove that the application of the oligomeric guluronic acid salts in Parkinson's disease prevention and treatment or improvement for the first time; and test results show that the oligomeric guluronic acid salts have no side toxic effects on nerve cells, is suitable for long term use, and has a wide market application prospect in the Parkinson's disease prevention and treatment.

It is described biologically pure bacterial cultures of mutant strains of Pseudomonas fluorescens, which produces large amounts of alginate. The alginate may contain a certain determined content of mannuronate and guluronate residues, possible presence and determined level of acetyl groups in the alginate, and a desired molecular weight of the alginate. Also high yielding mutants with regulation of alginate production, is described. The invention further provides methods for producing new mutant strains of Pseudomonas fluorescens and variants thereof, and use the resulting strains in alginate production.

The invention relates to a novel absorbable hemostatic material which is easy in preparation method, low in cost and good in hemostatic effect. At present most medical hemostatic materials in China are water-soluble hemostatic gauze, and the gauze does not have anti-inflammatory and anti-bacterial effects when being used for hemostasis, and has the defects that the hemostatic speed is low, the in vivo absorption time is long, an inflammatory reaction is easy to cause and the like, and therefore people turns to hemostatic powder, however, as existing hemostatic powder is complex to prepare, high in price, not ideal in hemostatic effect and the like, the hemostatic powder is not applied as a mainstream hemostatic product. The novel absorbable hemostatic material is a guluronic acid polymer generated by an complexation reaction or crosslinking reaction of a guluronic acid monomer, polyvinyl alcohol, lavender essential oil and clove essential oil. The novel absorbable hemostatic material is good in hemostatic effect, and has the advantages that the hemostatic speed is high, the in vivo absorption time is short, broad-spectrum sterilization and anti-inflammation effects are achieved, wound healing is promoted and the like, and in addition, the novel absorbable hemostatic material is not affected by the size and position of a wound surface, and widely applicable to the fast hemostasis for war wound, trauma and other conditions.

The invention relates to a mannuronic acid and guluronic acid imbedded oligosaccharin, characterized in that the non-deacidizing end has double bonds, the preparation comprises preparing algin into water solution, charging algin cracking enzyme for reaction, heating by boiling water bath, centrifuging to remove settled foreign substance, separating and purifying through chromatography.

The invention provides a low-molecular-weight polysaccharide sulfate and a preparation method and application of the low-molecular-weight polysaccharide sulfate. The constitutional formula of the low-molecular-weight polysaccharide sulfate includes that the molecular skeleton of the constitutional formula is a binary linear block polymer composed of beta-D-(1,4)-mannuronic acid and alpha-L-(1,4)-guluronic acid. In the constitutional formula, R=Na or CH2CHOHCH3, R1=H or SO3Na, in each saccharide link, at least one R1=SO3Na, m+n is smaller than or equal to 25, the weight-average molecular weight of the low-molecular-weight polysaccharide sulfate ranges from 3kDa to 9kDa, and dispersion of molecular weight distribution ranges from 1.20 to 1.70. The low-molecular-weight polysaccharide sulfatecan be applied to treatment of anticoagulation and anti-thrombus diseases, reduces risk of haemorrhagia and avoids anaphylaxis, and supplies raw materials for wide bioactivity of a series of low-molecular-weight PSS (polysaccharide sulfate) products.