63 results about "Aldehydic Acids" patented technology

The invention discloses a genetically engineered bacterium for producing glucuronic acid as well as a construction method and application of the genetically engineered bacterium, and belongs to the technical field of genetic engineering. According to the genetically engineered bacterium for producing glucuronic acid, an inositol oxidase gene MMIOX is integrated on a site of a uronic acid isomerase gene uxaC. According to the invention, an inositol oxidase gene MMIOX is integrated on a genome of a genetically engineered bacterium for producing glucuronic acid, and a uronic acid isomerase gene uxaC is knocked out at the same time, so that the genetically engineered bacterium can efficiently catalyze inositol reaction to generate glucuronic acid through the integrated inositol oxidase gene MMIOX; the glucuronic acid isomerase gene uxaC is knocked out, so that isomerization reaction of glucuronic acid can be avoided, and the yield of glucuronic acid is further increased.

The invention provides a method for extracting acacetin-7-O-glucuronide from dracocephalum moldavica, and relates to the technical field of medicine extraction. The method comprises the following steps: heating and extracting dracocephalum moldavica or dracocephalum moldavica products by adopting an alkaline solution, and precipitating the obtained extracting solution under the condition that the pH value is 1-3 to obtain a precipitate extract; mixing n-hexane, ethyl acetate, ethanol and water, and layering to obtain an upper phase solution and a lower phase solution; and dissolving the precipitate extract with a lower-phase solution, carrying out high-speed counter-current chromatography separation on the obtained sample solution, and collecting an acacetin-7-O-glucuronide fraction according to a chromatogram. According to the method, alkali extraction, acid precipitation and high-speed counter-current chromatography are combined, process conditions are optimized, the high-purity monomer of acacetin-7-O-glucuronide in dracocephalum moldavica is rapidly prepared, and the process is simple.

The invention discloses a lutein composition for relieving asthenopia and a preparation method of the lutein composition, and belongs to the field of functional foods. Phycocyanin and kelp polyphenol are mixed and react to obtain modified phycocyanin; fucoidin and beta-glucuronidase are mixed and react to obtain modified fucose; mixing the fish collagen peptide and zinc chloride to react to obtain a collagen peptide-zinc chelate; the xanthophyll siliceous cage compound and the modified phycocyanin are mixed and react to obtain a first compound; mixing and reacting the first compound and modified fucose to obtain a second compound; mixing and reacting the second compound, collagen peptide-zinc chelate and krill oil to obtain a lutein compound; mixing and reacting the second compound, collagen peptide-zinc chelate and krill oil, adding trehalose, and mixing and reacting to obtain a xanthophyll compound; and mixing the xanthophyll compound and maltodextrin to obtain the xanthophyll composition.

The present invention discloses a preparation method of glucurone, which relates to the technical field of esterification reactions. The method comprises the following steps: adding inositol oxidase to an inositol feed solution for conversion to obtain a conversion solution, and successively subjecting the conversion solution to filtration, ion exchange, decolorization, thermal concentration, and lactonization crystallization steps to obtain crude glucurone. The preparation method of the present invention uses inositol as a raw material, and after converting inositol into glucuronic acid solution under the action of an enzyme, adopts filtration, ion exchange, decolorization, thermal concentration, and lactonization crystallization steps to prepare crude glucurone. By adopting the technical solution of the present invention to prepare glucurone, the problems of low extraction rate of crude products, severe operating pressure, strict equipment requirements, and environmental pollution existing in the nitric acid oxidation method using starch as a raw material are solved. At the same time, the present invention uses inositol as a raw material, which is a new process different from the process using starch as a raw material, and has the advantages of low production cost, short cycle, and industrialization feasibility.

The compounds of the invention can be used to stabilize biomolecules, particularly in the presence of pH and thermal stress. Formula (I), wherein X is a hexosyl uronic acid or uronic acid amide group. (I).

The invention relates to a uridine diphosphate-glucose dehydrogenase mutant and an application of the uridine diphosphate-glucose dehydrogenase mutant in synthesis of uridine diphosphate-glucuronic acid. The amino acid sequence of the uridine diphosphate-glucose dehydrogenase mutant D7P166M / I169A is as shown in SEQ ID NO. 2, and the nucleotide sequence of the coding gene is as shown in SEQ ID NO. 1. The invention also provides an application of the mutant in preparation of uridine diphosphate-glucuronic acid, and a method for preparing uridine diphosphate-glucuronic acid by using the mutant. Experimental determination shows that the melting temperature of the mutant is increased by 30 DEG C compared with that of a wild type, the activity is improved by 20%, and a powerful tool is provided for enzymatic synthesis of uridine diphosphate-glucuronic acid compounds. Compared with a conventional synthesis pathway, the method for synthesizing uridine diphosphate-glucuronic acid through immobilized enzyme flow provided by the invention can be used for stably and efficiently synthesizing for a long time, and is beneficial to reducing the cost and realizing industrial synthesis.

Disclosed is a chemical synthesis method for Pseudomonas aeruginosa O5 serotype O-antigen oligosaccharide, which belongs to the field of chemical synthesis. In the disclosure, O-antigen trisaccharide is constructed with a D-glucuronic acid building block and a D-fucosamine building block, where the stereoselective synthesis of a 1,2-α-cis-glycosidic bond of D-fucosamine depends on remote acyl participation and reagent regulation, and synthesis of two types of 1,2-β-trans-glycosidic bonds of 2,3-diaminomannuronic acids is achieved via SN2 nucleophilic substitution of azido at position C2; and via selective assembly of protecting groups, orthogonal modification of modifying groups, and regulation of the reactivity of glycosyl donors and acceptors, multifunctional modified O-antigen target trisaccharide is successfully prepared. According to the method in the disclosure, the raw materials are cheap and easily available, and the preparation method is simple and easy to repeat. Therefore, the method has a very good application prospect in developing vaccines against P. aeruginosa.

The invention discloses a preparation method of a pyronaphthyridine metabolite, which comprises the following steps: reacting a compound A serving as a raw material with a compound B (2, 3, 4-tri-O-acetyl-alpha-D-glucuronide methyl ester trichloroacetimidate) under the action of a catalyst to obtain a compound C, and hydrolyzing the compound C under an alkaline condition to obtain a compound D, namely the pyronaphthyridine metabolite. The preparation method disclosed by the invention is reasonable in design, simple in post-treatment, easily available in raw materials and high in operability; the purity of the prepared target product can reach 97.0% or above, the method can be used for pharmacokinetic research, a test sample is provided for research on the metabolic mechanism of the pyronaphthyridine, and the method has important application value.

A method for extracting flavonoid components in Dracocephalum moldavica L., comprising the following steps: mixing Dracocephalum moldavica L. and an alkaline solution for heating extraction; under the condition that the pH value is 1-3, mixing an obtained extract solution with acid for precipitation; dissolving an obtained precipitated extract and then carrying out preparative liquid chromatography separation; and respectively concentrating obtained Fr1-Fr6 fractions until the fractions are dry, so as to sequentially obtain luteolin-7-O-β-D-glucuronide, apigenin-7-O-β-D-glucuronide, diosmetin-7-O-β-D-glucuronide, tilianin, acacetin-7-O-glucuronide, and acacetin.

The invention discloses a method for detecting glucuronic acid in a calcium gluconate bulk drug. The method has high system applicability, has incomparable advantages in specificity, quantitation limit, detection limit, linear range, repeatability and durability, and has high precision.

The invention discloses a method for detecting various pollutants in follicular fluid. The method comprises the steps of enzymolysis, extraction, nitrogen blowing, redissolution and detection on a machine. Mixing the follicular fluid sample with the mixed internal standard solution, performing enzymolysis overnight, and hydrolyzing the bound metabolite by using beta-glucuronidase; extracting by using an ethyl acetate / methyl tert-butyl ether mixed solution containing 0.1% formic acid, and repeating for three times to extract 13 types of 182 pollutants; and carrying out nitrogen blowing drying and acetonitrile / water redissolution, and analyzing through a series quadrupole rod liquid chromatograph-mass spectrometer. According to the method, the detection limit is as low as nanogram / milliliter, quantification is completed within a short time through single sample injection, more than 100 samples can be detected in a single day, high-throughput and high-sensitivity detection is achieved, energy consumption and cost are reduced through program optimization, high efficiency and environmental friendliness are achieved, and a powerful means is provided for studying the follicular fluid pollutant exposure level and correlating with reproductive health.

The invention relates to a uridine diphosphate-glucuronyl transferase mutant and an application of the uridine diphosphate-glucuronyl transferase mutant in catalytic synthesis of glucuronide. The amino acid sequence of the uridine diphosphate-glucuronyl transferase mutant is as shown in SEQ ID NO. 2, and the nucleotide sequence of the coding gene is as shown in SEQ ID NO. 1. The invention provides a uridine diphosphate-glucuronyl transferase mutant with high activity and high stability. Compared with wild uridine diphosphate-glucuronyl transferase, the uridine diphosphate-glucuronyl transferase mutant has mutation of 10 amino acid sites. According to experimental determination, the melting temperature of the mutant is increased by 24 DEG C compared with that of a wild type, the activity is increased to 1.65 times, the expression quantity is increased to 4.2 times, and a powerful tool is provided for enzymatic synthesis of glucuronide compounds.

The invention relates to the technical field of component detection of clerodendrum japonicum, in particular to a method for determining the content of active substances in clerodendrum japonicum based on a high performance liquid chromatography method. The invention provides a method for determining the content of active substances in clerodendrum japonicum based on high performance liquid chromatography, which comprises the following steps: (1) mixing the clerodendrum japonicum medicinal material with ethanol, and carrying out ultrasonic treatment to obtain a test solution; the method comprises the following steps: respectively taking a chlorogenic acid solution, an apigenin-7-O-diglucuronic acid solution, a verbascoside solution and an acacetin-7-O-[beta-D-glucopyranuronyl (1-> 2)-O-D-glucopyranuronide] solution as reference substance solutions; and (2) performing high performance liquid chromatography detection on the test solution and the reference solution obtained in the step (1). The method can be used for simultaneously detecting the contents of four active components in clerodendrum japonicum, is low in detection limit and wide in linear range, and ensures the accuracy and reliability of a detection result.

Compounds comprising a cross-linking moiety and a protecting group are described herein along with their methods of use. The cross-linking moiety may comprise an indoxyl and the protecting group may comprise a sugar (e.g., a glucuronide or glucoside), phosphoester, or sulfoester group. The cross-linking moiety and protecting group may be attached to each other via an oxygen atom, sulfur atom, or linker. In some embodiments, the linker attaching the cross-linking moiety and protecting group is a self-immolative linker. A compound of the present invention may cross-link under physiological conditions and / or in vivo.

The application discloses a kind of high-efficiency interception polyvalent anion and cation nanofiltration membrane and its preparation method and application.The method includes the following preparation process:1) preparation concentration is 0.1-2wt% of apigenin class of substance aqueous solution, and concentration is 0.1-0.5wt% of multi-acid chloride organic phase solution;2) make polymer support membrane in aqueous solution 1-5min, remove the surface excess solution, then immerse into oil phase solution, react 1-15min;3) after reaction, remove the surface excess solution, solidify 5-20min under 60-80 DEG C, obtain the nanofiltration membrane.The application is with apigenin, apigenin-7-o-glucuronide and / or isofraxidin as aqueous monomer, and multi-acid chloride is prepared by interfacial polymerization reaction to obtain negative NF membrane, not only maintain the high interception rate to polyvalent anion, and also have very high interception rate to polyvalent cation, can efficiently permeate monovalent ion, and water flux is larger.

This invention relates to an aqueous emulsion comprising: (A) an ester of fatty acids and polyglycerol having 16 to 22 carbon atoms, an ester of fatty acids and glycerol having 16 to 22 carbon atoms, and a monohydric alcohol having 16 to 22 carbon atoms; (B) a compound having at least two hydroxyl groups and an alkyl group having 16 to 22 carbon atoms and having no phosphorus atoms, or a salt thereof, wherein component (B) is different from component (A); and (C) a cellulose thickener and / or a polysaccharide thickener comprising glucose, glucuronic acid, and rhamnose as the main chain of constituent monosaccharides.

This invention relates to the field of biotechnology, specifically to an inositol oxidase mutant and its application in the production of glucuronic acid. The inositol oxidase mutant provided by this invention is obtained by point mutation of the amino acid sequence shown in the wild-type inositol oxidase. Compared with the wild-type enzyme, the inositol oxidase mutant constructed by this invention exhibits a significant improvement in catalytic efficiency. Furthermore, this invention also provides an engineered strain with site-directed mutagenesis and knockout of the UxaC gene in the chassis cell genome. This strain exhibits higher catalytic activity towards the substrate, with a catalytic efficiency increased by 74.44% compared to the wild-type enzyme. Even at high substrate concentrations, the conversion rate can still reach over 92.09%, with a production intensity as high as 13.23 g / L / h. It possesses extremely strong substrate tolerance and production intensity, significantly reducing the production cost of glucuronic acid and demonstrating significant industrial application potential.

A method for the production of 2,5-furandicarboxylic acid (FDCA) comprising contacting glucose with a catalyst composition under conditions suitable for the formation of glucarate; acidifying glucarate to form glucaric acid; dehydrating glucaric acid to form FDCA; and recovering FDCA. A method for the production of 2,5-furan dicarboxylic acid dimethyl ester (FDME) comprising contacting glucose with a catalyst composition under conditions suitable for the formation of glycolaldehyde; contacting glycolaldehyde with a transition metal oxidation catalyst under conditions suitable for the formation of glucaric acid; esterifying glucaric acid to form glucaric acid methyl ester; dehydrating the glucaric acid methyl ester to form FDME; and recovering FDME.

A method for synthesizing an aluminium-fluoride complex obtainable by reacting together: a salt made of aluminium(III) and counterions of aluminium(III) other than chloride(I), and a fluoride anion; or a chelate bearing molecule precomplexed with aluminium(III), obtained from treating the chelate bearing molecule with a salt made of aluminium(III) and counterions of aluminium(III) other than chloride(I), and a fluoride anion; the fluoride being [19F]fluoride or radioactive [18F]fluoride; wherein the counterions of aluminium(III) other than chloride are selected from bromide, iodide, nitrate, phosphate, sulfate, sulphonate, hydroxide, any anionic salt of natural and non-natural amino acids, 2,4-pentanedionate, 8-hydroxyquinoline, acetate, acrylate, ascorbate, aspartate, benzenesulfonate, benzoate, besylate, bitartrate, camphorsulfonate, citrate, decanoate, esylate, fumarate, gentisate, gluconate, glutamate, glutarate, glycinate dihydroxide, glycolate, hexanoate, hydroxynaphthoate, isethionate, lactate, lactobionate, malate, maleate, malonate, mandelate, mesylate, methylsulfate, methylsulfonate, mucate, napsylate, octanoate, oleate, oxalate, pamoate, pantothenate, phthalate, polygalacturonate, propionate, salicylate, stearate, succinate, tartrate, teoclate, toluenesulfonate, trifluoroacetate, trifluoromethanesulfonate, and any combination thereof.

The invention relates to the technical field of determination methods, in particular to a liquid chromatography for determining a compound electrolyte injection (V), which comprises the following steps of: 1, debugging a liquid chromatograph, and adjusting the chromatography of the liquid chromatograph to be that sulfonic acid type polystyrene and divinylbenzene copolymer cation exchange resin H type is used as a filling agent; a 0.025 mol / L sulfuric acid solution is used as a mobile phase; the flow rate is 1.0 ml per minute; the column temperature is 60 DEG C; the detection wavelength is 210 nm; the sample injection volume is 100 to 250 [mu] l; 2, preparing a reference substance solution corresponding to the compound electrolyte injection (V), injecting the reference substance solution into a liquid chromatograph, and recording a corresponding chromatogram; and 3, injecting the compound electrolyte injection (V) to be detected into the liquid chromatograph, recording a corresponding chromatogram, and obtaining the contents of sodium gluconate and sodium acetate and the contents of D-glucuronic acid and 5-hydroxymethyl-2-furoic acid in the compound electrolyte injection (V) through a formula.

The invention discloses a high-light-transmittance contact lens silicone hydrogel material and a preparation method thereof, and relates to the technical field of contact lens materials, the high-light-transmittance contact lens silicone hydrogel material comprises the following raw materials by weight: 25-35 parts of a silane monomer, 20-25 parts of a functional monomer, 0.6-1.0 part of a cross-linking agent, 0.3-0.5 part of an initiator, and 40-50 parts of a solvent. The silane monomer, the functional monomer and the cross-linking agent are combined for use, so that the high light transmittance of the prepared silicon hydrogel material meets the use requirement. The functional monomer is prepared from 4-nitrophenyl-beta-D-glucuronic acid sodium salt, 2-cyano-5-(dimethylamino)-3-ethoxy penta-2, 4-dienoic acid ethyl ester, 5-chloro-2-pentanone and the like through a reaction, and the contact lens silicon hydrogel material is endowed with relatively high water content and relatively strong antibacterial property.

The present disclosure provides drilling fluids including emulsions. The emulsions include an aqueous phase, an oil, an emulsifier composition, and a wetting agent. The emulsifier composition includes an amidoamine. The wetting agent includes a first organic acid. The first organic acid selected from the group consisting of C6-C30 carboxylic acid, C6-C30 alkeynl acid, C6-C30 arylakyl acid, and a C6-C30 cycloalkyl acid. The wetting agent includes an alcohol alkoxylate. A ratio of the oil to the aqueous phase is about 85:15 to about 60:40 of the oil to the aqueous phase

The invention discloses a method for synthesizing a pevitide key intermediate, and relates to the technical field of medicine synthesis. The specific preparation method comprises the following steps: firstly, reacting 2, 3, 4, 6-tetraacetoxy-alpha-D-glucopyranose bromide with 18-hydroxy tert-butyl octadecanoate under the action of a catalyst to generate a glycoside intermediate, and carrying out alkaline hydrolysis on the glycoside intermediate to remove an acetyl protecting group, so as to obtain the 2, 3, 4, 6-tetraacetoxy-alpha-D-glucopyranose. And finally, carrying out oxidation reaction and purification to obtain a target product 18-([beta-D-glucuronic acid-1-yl] oxy) tert-butyl octadecanoate, namely the pevitide key intermediate. According to the preparation method disclosed by the invention, the pevitide key intermediate with relatively high yield and purity can be finally obtained, and the preparation method is simple to operate and has a good application prospect.

The invention relates to oligosaccharides composed of alternating glucuronic and N-acetylglucosamine units, with a β-D-glucuronic, β-D-galacturonic or β-L-altruronic unit at the non-reducing end with a substituent attached by a sulfide bond according to the structural formula (I), (II) or (III), their preparation and method of use. The invention also relates to a pharmaceutical composition comprising the oligosaccharide according to the structural formula (I), (II) or (III). Formulae for abstract (formulae I, II and III) wherein n = 0 to 8; X is O or N-H; Y is H, Na, K or aliphatic, aromatic, linear, branched or cyclic chain C2-C7; R is aliphatic, aromatic, heteroaromatic, linear, branched or cyclic chain C2-C70, optionally with content of N, S, Cl or O atoms.

The present disclosure provides a 3-phenoxybenzoic acid-glucuronic acid conjugate, and a preparation method and use thereof, and belongs to the technical field of pesticide detection. Compared with 3-phenoxybenzoic acid, the 3-phenoxybenzoic acid-glucuronic acid conjugate provided by the present disclosure features structural stability, high specificity, long limit of detection, and high content in urine, and can better serve as a marker that identifies whether an organism is killed due to pyrethroid pesticide poisoning. Namely, the 3-phenoxybenzoic acid-glucuronic acid conjugate can detect whether a toxicant (pyrethroid pesticides) is taken antemortem or exposed postmortem, and has an excellent application prospect in pyrethroid pesticide detection. The present disclosure provides a preparation method of a 3-phenoxybenzoic acid-glucuronic acid conjugate. The preparation method provided by the present disclosure features high product yield, simple operation, wide raw material sources, low costs, and suitability for industrial production.

To provide a method for industrially and stably producing acidic hypochlorous acid water having a stable pH.SOLUTION: A production method for acidic hypochlorous acid water includes an ion exchange step of passing an aqueous sodium hypochlorite solution having an effective chlorine concentration of 0.5 mass% or more through a column packed with a weakly acidic cation-exchange resin. The weakly acidic cation-exchange resin has a water retaining capacity of 60% or less. Product collection is started from a time point when the pH of passing liquid at a column outlet reaches 2.5, and the collection is stopped at a time point when the pH reaches 5.5 or less.SELECTED DRAWING: Figure 1

The invention provides a high-adhesion high-stretchability self-healing hydrogel capable of being rapidly formed and a preparation method thereof. The preparation method comprises the following steps: reacting glycidyl methacrylate with polyvinyl alcohol to introduce double bonds, and then adding succinic anhydride to introduce carboxyl to prepare carboxylated methacrylated polyvinyl alcohol. 3-aminophenylboronic acid is used for modifying glycyrrhizic acid, and phenylboronic acid dipotassium glycyrrhizinate is obtained. Phenylboronic acid groups, hydroxyl groups and double bond crosslinking serve as main acting force, the hydrophilic and hydrophobic effect (glycyrrhizic acid is composed of one-molecule hydrophobic glycyrrhetinic acid and two-molecule hydrophilic glucuronic acid) serves as auxiliary acting force, the gelation time is remarkably shortened, and the mechanical performance of the hydrogel is improved. Moreover, due to the special molecular structure of glycyrrhizic acid and a large number of hydroxyl groups in the system, the glycyrrhizic acid has very good adhesion performance with different substrates, and the hydrogel has good self-healing performance and tensile property.

The present invention discloses a method for simultaneously determining multiple chemical components in Cymbidium heterophyllum, and relates to the technical field of separation and analysis of natural products. Ultra-high performance liquid chromatography is used to simultaneously determine the chemical components of Cymbidium heterophyllum, and the chemical components include at least neochlorogenic acid, cryptochlorogenic acid, and diosgenin-7-O-glucuronide; the detection conditions of ultra-high performance liquid chromatography include: chromatographic column stationary phase: diisobutyl group is bonded to the silica gel surface through siloxane; mobile phase A is selected from methanol or acid-containing methanol, and mobile phase B is selected from water or acid-containing aqueous solution; mobile phase A and mobile phase B are used as a mixed mobile phase for gradient elution. The present invention establishes a UPLC content determination method for multiple chemical components in Cymbidium heterophyllum, which is simple to operate, has reliable results, and good repeatability, and can provide a certain reference for the determination of the content of chemical components in Cymbidium heterophyllum and the improvement of existing quality standards.

A gel composition, a preparation method therefor and a use thereof. The gel composition comprises a gel and an emulsifier; the gel comprises gellan gum; the emulsifier comprises pectin; the pH of the diluent of the gel composition is 2.75-3.5; the diluent is obtained by mixing the gel composition and water at the mass ratio of 1:10; and the weight-average molecular weight of the pectin is 10000-500000 daltons, and / or the acetylation degree of the same-type galacturonic acid main chain in the pectin is 15-30%.