40 results about "Galactose oxidase" patented technology

The invention discloses a high content modified guar gum solution manufacture method. The modified guar gum powder could be hydroxide propyl guar gum, positive ion guar gum or the mixture. It includes the following steps: adding positive ion oxidation guar gum powder into water to make the viscosity over 3000mpa.s, adding mannose enzyme solution, adding galactose oxidase solution, heating to 40-60 degree centigrade and reacting for 4-8 hours to gain modified guar gum solution that has viscosity at about 200-500mpa.s, content is 10-25%. The invention could be used to make paper making sizing agent. Comparing to simply using AKD, the sheet and the Cobb value after aging is obviously decreased.

A hydrocarbon-bearing subterranean formation may be treated with an aqueous well treatment fluid which contains a hexose oxidase, such as glucose oxidase, mannose oxidase or galactose oxidase. The aqueous well treatment fluid further may contain a viscosifying polymer and an aldohexose. The aldohexose reacts in-situ with the hexose oxidase and molecular oxygen to produce hydrogen peroxide. The hydrogen peroxide may then act as a breaker.

The invention discloses a high content modified guar gum solution and the manufacture method. It includes the following steps: adding positive ion oxidation guar gum powder into water, adding pH retarding agent to adjusting pH value of the system is 7-8, adding mannose enzyme solution, adding galactose oxidase solution, heating to 40-60 degree centigrade and reacting for 4-8 hours, heating to 90-95 degree centigrade keeping for 10-50 minutes to gain modified guar gum solution. The invention could be used to make paper making sizing agent. Comparing to simply using AKD, the sheet and the Cobb value after aging is obviously decreased.

Polysaccharides comprising one or more oxime linkages are provided. Methods for their preparation include the polycondensation of saccharides bearing oxime-forming substituents. In some embodiments, polymerization is conducted in the presence of galactose oxidase. The resulting oxime-linked polysaccharides have desirable properties and are useful in numerous applications including paper manufacturing and drug delivery vehicles.

The invention discloses a reagent card used for detecting faeces lactose contents. The reagent card comprises a sample pad, a reaction pad and a color development pad, wherein the sample pad, the reaction pad and the color development pad are connected in sequence; the sample pad bears lactase, the reaction pad bears galactose oxidase, and the color development pad bears color developing agent. Byuse of the reagent card, lactose in the faeces can be effectively measured, and therefore, the reagent card is simple in clinical operation and high in specificity. The invention also discloses a faeces lactose content detection method.

The invention discloses a cell surface glycan in-situ electrogenerated fluorescence imaging analysis method based on a bipolar electrode. The method comprises the following steps: 1) preparing a bipolar electrode micro-fluidic chip; (2) preparing a Bio-DNA (deoxyribonucleic acid)-AuNPs (gold nanoparticles)-Fc-DNA (deoxyribonucleic acid) nanoprobe; 3, incubating HepG2 cells in situ in an anode channel of the bipolar electrode, adding galactose oxidase to oxidize hydroxyl on galactose on the surfaces of the cells into aldehyde groups, aniline catalyzes a biotin hydrazide reaction to form a hydrazone compound, avidin serves as a bridge, and the hydrazone compound is combined with the Bio-DNA-AuNPs-Fc-DNA nanoprobe; 4) adding azure molecules into a cathode pool of the bipolar electrode, oxidizing Fc introduced by the anode under the potential of 2.4 V, reducing azure of the cathode into high-fluorescence resorufin, and quantitatively detecting the galactose expression level on the surfaceof the anode cell through the fluorescence intensity of the cathode; according to the method, the damage of fluorescence labeling or mass spectrometry to cells is avoided by utilizing a mild enzymaticoxidation method, and a sensitive electrochemical signal is converted into a visual optical signal by combining the bipolar electrode, so that high-resolution fluorescence imaging is realized.

This disclosure relates to a screening test method, device, and kit for carbohydrates found in a biological sample and associated conditions including, cancerous and precancerous conditions. Specifically, the method tests abnormal carbohydrates in a biological sample using reagents of galactose oxidase, and Schiff's Reagent. The screening test method, device, and kit provides expanded testing capabilities across a range of known conditions and in an otherwise healthy population. This disclosure further relates to the use of the device or kit for an initial evaluation for cancerous and precancerous conditions in people without obvious signs and symptoms, and cancer recurrence in at point-of-care facility or at home.

The invention relates to a kit for diagnosing/measuring sodium (ions) by utilizing the technologies of the enzymic colorimetry and the enzyme linked immunosorbent assay. The invention further relates to a method, a principle and the composition and the components of a reagent for measuring the concentration of sodium (ions), and belongs to the technical field of medical/food/environmental inspection and measurement. The main components of the kit include a buffer solution, reduced coenzyme, lactose, beta-galactosidase, galactose oxidase, NADH oxidase and a stabilizer. Through mixing a sample and the reagent by a certain volume ratio, a series of enzymatic reactions occur, then the reactant is placed under an ultraviolet/visible light analyzer, and the degree/velocity of the decrease in absorbance at 340 nm of the dominant wavelength is detected, thereby measuring the concentration of sodium (ions).

The invention relates to a reagent (kit) for diagnosing/determining amino acid by using an enzyme colorimetric method and an enzyme link method, and also discloses a method for determining the concentration of the amino acid, a composition and components of the reagent, belonging to the technical field of medicine/food/environmental test determination. The reagent (kit) comprises the main components: a buffer solution, a coenzyme, a galactose hexose dialdose, an amino acid oxidase, a galactose oxidase, a galactose dehydrogenase and a stabilizing agent. A sample and the reagent are mixed according to a certain volume ratio to generate a series of enzymatic reactions, and reactants are placed under an ultraviolet/visible light analyzer for detecting the degree of absorbance rise at the dominant wavelength of 340 nm, thereby measuring and calculating the concentration of the amino acid.