Method for determining galactose concentration and galactose diagnosis/determination reagent kit

A technology for concentration determination and galactose, which is applied in the field of medical/food inspection and determination, can solve problems such as element absorption disorders, affecting infants' physical and intellectual development, etc.

Inactive Publication Date: 2008-12-17
SUZHOU ANJ BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003]Although milk is good, not all people can enjoy it. Due to the lack of lactase, the human body cannot digest and absorb the nutrients in milk well, and will Causes absorption disorders of calcium, phosphorus, potassium, iron and other elements, affecting the physical and intellectual development of infants and young children. In the elderly, lactase deficiency is an important cause of osteoporosis

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0093] The galactose diagnosis / measurement reagent of this embodiment is a single reagent, including:

[0094] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0095] Stabilizer 500mmol / L

[0096] Peroxidase 30000U / L

[0097] Galactose oxidase 12000U / L

[0098] 4-amino antiarsenic 2mmol / L

[0099] Carbolic acid 10mmol / L

[0100] After the reagents are all dissolved and prepared, they are divided into bottles and freeze-dried to make dry powder reagents; before use, add purified water and reconstitute for use.

[0101] Set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, test main wavelength 505nm, test sub-wavelength 600nm, the volume ratio of the tested galactose sample to the reagent is 1 / 25, and the reaction direction is positive reaction ( Ascent reaction), the detection method is the endpoint / rate method, the delay time is about 0 minutes, and the detection time is about 5 minutes.

[0102] After adding the sampl...

Embodiment 2

[0104] The galactose diagnosis / measurement reagent of this embodiment is a double reagent, comprising:

[0105] Reagent 1

[0106] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0107] Stabilizer 50mmol / L

[0108] 2,4,6-tribromo-3-hydroxy-benzenesulfonic acid 0.2mmol / L

[0109] Reagent 2

[0110] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0111] Stabilizer 500mmol / L

[0112] Peroxidase 30000U / L

[0113] Galactose oxidase 12000U / L

[0114] 4-amino antiarsenic 0.6mmol / L

[0115] After the reagents are all dissolved and prepared, they are divided into bottles to make liquid double reagents, which can be used directly.

[0116] Set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, test main wavelength 546nm, test sub-wavelength 600nm, the volume ratio of the measured galactose sample to the reagent is 1 / 20, and the reaction direction is positive reaction ( Ascent reaction), the detection meth...

Embodiment 3

[0119] The galactose diagnosis / measurement reagent of the present embodiment is three reagents, comprising:

[0120] Reagent 1

[0121] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0122] Stabilizer 50mmol / L

[0123] 3-methyl-2-benzothiazolone hydrazone 0.6mmol / L

[0124] Reagent 2

[0125] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0126] Stabilizer 50mmol / L

[0127] Dimethylaniline 2mmol / L

[0128] Reagent 3

[0129] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0130] Stabilizer 500mmol / L

[0131] Peroxidase 30000U / L

[0132] Galactose oxidase 12000U / L

[0133] After all the reagents are dissolved and prepared, they are divided into bottles to make liquid three reagents, which can be used directly.

[0134] Set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, test main wavelength 578nm, test sub-wavelength 660nm, the volume ratio of the measured galactose sa...

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Abstract

The invention relates to a kit for diagnosing / mensurating galactose by utilizing the technologies of the enzymic colorimetric method and the enzyme linked immunosorbent assay (ELISA). The invention further relates to a method, a principle and the composition and the components of a reagent for mensurating the concentration of the galactose, and belongs to the technology field of medical / food inspection and measurement. The main components of the kit include a buffer solution, galactose oxidase, peroxidase, a reduced chromogen combination and a stabilizer. Through a series of enzymatic reactions, the colorless reduced chromogen combination is finally oxidized into the colored dyes, thereby mensurating the content of the dyes at 400nm-700nm of the wavelength by a visible light analyzer and further reflecting the concentration of the galactose directly.

Description

technical field [0001] The invention relates to a galactose diagnosis / measurement kit, and at the same time, the invention also relates to a method for measuring galactose concentration, which belongs to the technical field of medicine / food inspection and determination. Background technique [0002] Milk is known as "liquid gold" in the nutritional field. As early as 1992, seven departments in my country jointly proposed the slogan "a glass of milk strengthens a nation". Except for cellulose, milk contains almost all kinds of nutrients needed by the human body. After lactose in milk enters the human body, it is decomposed into glucose and galactose by the action of lactase in the small intestine. Galactose is an essential substance for the development of the baby's brain and is closely related to the rapid growth of the baby's brain. [0003] Although milk is good, not all people can enjoy it. Due to the lack of lactase, some people cannot digest and absorb the nutrients i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/66G01N21/31C12Q1/26C12Q1/00
Inventor 王尔中
Owner SUZHOU ANJ BIOTECHNOLOGY CO LTD
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