Reagent card used for detecting faeces lactose contents, and faeces lactose content detection method based on reagent card

Abstract

The invention discloses a reagent card used for detecting faeces lactose contents. The reagent card comprises a sample pad, a reaction pad and a color development pad, wherein the sample pad, the reaction pad and the color development pad are connected in sequence; the sample pad bears lactase, the reaction pad bears galactose oxidase, and the color development pad bears color developing agent. Byuse of the reagent card, lactose in the faeces can be effectively measured, and therefore, the reagent card is simple in clinical operation and high in specificity. The invention also discloses a faeces lactose content detection method.

Description

technical field [0001] The invention relates to the field of lactose detection, in particular to a reagent card for detecting lactose content in feces and a method for detecting lactose in feces based on the reagent card. Background technique [0002] Lactose is a disaccharide with the molecular formula C 12 h 22 o 11 , is a disaccharide in mammalian milk, hence the name. Its molecular structure is formed by the condensation of a molecule of glucose and a molecule of galactose, which is divided into two isomers, α-type and β-type. α-lactose can be decomposed into glucose and galactose by acid and lactase, and can be tolerated by lactic acid bacteria. It becomes lactic acid through the action of species, but yeast cannot use it. β-lactose only exists in animal milk, about 7% (5% to 8%) in human milk, and about 4.5% (4% to 5%) in cow milk. At body temperature, two parts of α- Type and three β-type ratio mixed to form an equilibrium state. [0003] Lactose cannot be absor...

AI Technical Summary

Problems solved by technology

This method is accurate, simple, non-invasive and convenient, and its specificity and sensitivity are >90%. It is beneficial to the investigation of large sample population; 3) About 2% of normal population does not produce H 2 ; 4) Diabetes and other factors affect the test results; 5) It requires the cooperation of the subjects, and young children (<3 years old) cannot do it; 6) For positive patients, it is necessary to exclude the presence of disaccharide intolerance such as sucrose; 7) Lack of uniformity Oral dosage of lactose and judging criteria for positive test results

However, both require sample pretreatment and heating in a high-temperature water bath, which is complicated in clinical operations, has poor specificity, and is difficult to realize automation. In addition, the lead acetate method and the improved Benedictine reagent method both have heavy metal pollution and biosafety risks, and the defects are very obvious.