Test paper for detecting lactose content of sample and preparation method of test paper

Abstract

Test paper for detecting lactose content of a sample and a preparation method of the test paper are provided; the test paper is made by: selecting filter paper, making a substrate carrier, adding lactase to the filter paper, drying by using a drying technique, adding TMB, horse radish peroxidase and galactose oxidase sequentially to another filter paper, drying step by step, selecting a PVC (polyvinyl chloride) board to fix the dried filter paper, and cutting; the test paper made herein is suitable for detecting the presence of lactose in a sample; as enzymatic reaction is used, the reaction is quick and the sensitivity is high; by using test paper method, it is possible to finish detection by one-time sample adding; results are judged through color variation so that detection is simple and easy; the preparation method of the test paper is simple, the cost is low, and the test paper is easy to popularize and suitable for quickly detecting whether a sample contains lactose or not.

Description

[0001] Technical field: [0002] The invention relates to a detection test paper, which belongs to the technical field of detecting whether a sample contains lactose, such as a stool sample, and is especially suitable for quickly detecting whether a stool sample contains lactose and a preparation method of the test paper. [0003] Background technique: [0004] Lactose is mainly derived from the milk of mammals, and the lactose content of each mammal's milk is different. For example, human milk contains 7% lactose, cow's milk contains 4.2% lactose, and goat milk contains 4.6% lactose. Milk is a very nutritious drink, and it is the best substitute for breast milk for infants and young children. Milk contains protein, carbohydrates, phospholipids, calcium, zinc and other trace elements. Drinking milk can supplement nutrients such as protein and trace elements such as calcium and zinc; reduce the incidence of rickets, osteoporosis, anemia, cardiovascular diseases and other diseas...

AI Technical Summary

Problems solved by technology

[0008] At present, the technical patent 200610056826.5 of the dry chemical method for detecting lactose intolerance has been published, which uses the detection of galactose in urine to judge the basis of lactose intolerance. The limitations of the above patent are: 1) It detects human urine The galactose in the galactose needs to be added to the urine purification device, which increases the detection steps and cost; 2) The galactose oxidase pad, peroxidase pad, and chromogenic reagent pad are made separately and stacked together. After adding the sample, pass The forward chromatography reaction of each test paper is not intuitive enough

3) The above-mentioned technology cannot detect whether lactose is contained in other samples such as feces, and has limitations in the detection of samples

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