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Sodium (ion) diagnosis/measuring reagent kit and sodium (ion) concentration determination method

A kit and reagent technology, applied in the field of medical/food inspection and determination, can solve the problems of large errors, complicated operations, and the inability of biochemical analyzers to simultaneously measure the content of sodium ions and potassium ions

Inactive Publication Date: 2009-06-24
SUZHOU ANJ BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because there are holes in the macrocyclic structure, the oxygen atoms in the molecule have unshared electron pairs that can be combined with metal ions. According to the size of the holes, metal ions of different diameters can be selectively combined to achieve the purpose of measuring the ion concentration; Spectrophotometry can also be used to detect K in serum + 、Na + , but the operation is complicated, the error is large, and it is not as simple as the flame photometry
[0007] The method of enzymatic determination of sodium ion content has a good consistency with the flame photometer method or ion selective electrode method. This method has strong anti-interference and good stability, but the biochemical analyzer cannot simultaneously measure sodium ion and The respective content of potassium ions makes this method unable to be popularized and applied.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] The sodium (ion) diagnosis / measurement reagent of the present embodiment is a single reagent, comprising:

[0043] Tris(carboxymethyl)aminomethane—hydrochloric acid buffer 100mmol / L

[0044] Stabilizer 500mmol / L

[0045] Reduced coenzyme 0.25mmol / L

[0046] β-Galactosidase 6000U / L

[0047]Galactose oxidase 8000U / L

[0048] NADH peroxidase 10000U / L

[0049] Lactose 20mmol / L

[0050] After the reagents are all dissolved and prepared, they are divided into bottles and freeze-dried to make dry powder reagents; before use, add purified water and reconstitute for use.

[0051] Set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, initial absorbance 1.8±0.7, test main wavelength 340nm, test subwavelength 405nm, volume ratio of measured sodium (ion) sample to reagent is 1 / 25, the reaction direction is negative reaction (downward reaction), the delay time is about 2 minutes, and the detection time is about 2 minutes.

[0052] After adding...

Embodiment 2

[0054] The sodium (ion) diagnosis / measurement reagent of the present embodiment is a double reagent, comprising:

[0055] Reagent 1

[0056] Tris(carboxymethyl)aminomethane—hydrochloric acid buffer 100mmol / L

[0057] Stabilizer 50mmol / L

[0058] Reduced coenzyme 0.25mmol / L

[0059] Lactose 20mmol / L

[0060] Reagent 2

[0061] Tris(carboxymethyl)aminomethane—hydrochloric acid buffer 100mmol / L

[0062] Stabilizer 500mmol / L

[0063] β-Galactosidase 6000U / L

[0064] Galactose oxidase 8000U / L

[0065] NADH peroxidase 10000U / L

[0066] After the reagents are all dissolved and prepared, they are divided into bottles to make liquid double reagents, which can be used directly.

[0067] Set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, initial absorbance 1.8±0.7, test main wavelength 340nm, test subwavelength 405nm, measured sodium (ion) sample and reagent 1, reagent 2 The volume ratio is 2 / 20 / 5, the reaction direction is negative reaction ...

Embodiment 3

[0070] The sodium (ion) diagnosis / determination reagent of the present embodiment is three reagents, comprises:

[0071] Reagent 1

[0072] Tris(carboxymethyl)aminomethane—hydrochloric acid buffer 100mmol / L

[0073] Stabilizer 50mmol / L

[0074] Reduced coenzyme 0.25mmol / L

[0075] Lactose 20mmol / L

[0076] Reagent 2

[0077] Tris(carboxymethyl)aminomethane—hydrochloric acid buffer 100mmol / L

[0078] Stabilizer 500mmol / L

[0079] Galactose oxidase 8000U / L

[0080] NADH peroxidase 10000U / L

[0081] Reagent 3

[0082] Tris(carboxymethyl)aminomethane—hydrochloric acid buffer 100mmol / L

[0083] Stabilizer 500mmol / L

[0084] β-Galactosidase 6000U / L

[0085] After all the reagents are dissolved and prepared, they are divided into bottles to make liquid three reagents, which can be used directly.

[0086] When measuring sodium (ion) concentration, set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, initial absorbance 1.8±0.7, test main wa...

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PUM

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Abstract

The invention relates to a kit for diagnosing / measuring sodium (ions) by utilizing the technologies of the enzymic colorimetry and the enzyme linked immunosorbent assay. The invention further relates to a method, a principle and the composition and the components of a reagent for measuring the concentration of sodium (ions), and belongs to the technical field of medical / food / environmental inspection and measurement. The main components of the kit include a buffer solution, reduced coenzyme, lactose, beta-galactosidase, galactose oxidase, NADH oxidase and a stabilizer. Through mixing a sample and the reagent by a certain volume ratio, a series of enzymatic reactions occur, then the reactant is placed under an ultraviolet / visible light analyzer, and the degree / velocity of the decrease in absorbance at 340 nm of the dominant wavelength is detected, thereby measuring the concentration of sodium (ions).

Description

technical field [0001] The invention relates to a sodium (ion) diagnosis / measurement kit, and at the same time, the invention also relates to a method for measuring sodium (ion) concentration, which belongs to the technical field of medicine / food inspection and determination. Background technique [0002] The content of sodium ions in serum (referred to as "blood sodium") has very important clinical significance. There are three decision levels in medicine, in order: level 1—115mmol / l, level 2—135mmol / l, level 3— -150mmol / l. When the patient's blood sodium concentration is equal to or lower than level 1, symptoms such as confusion, fatigue, nausea, headache, vomiting, and anorexia may appear, indicating that the proportion of water in the body is greater than sodium, and corresponding treatment measures should be taken; when the blood sodium concentration When it is lower than level 2, the cause of hyponatremia should be found, and the serum osmotic pressure, blood potassiu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/31G01N33/84C12Q1/00
Inventor 王尔中
Owner SUZHOU ANJ BIOTECHNOLOGY CO LTD
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