541 results about "Enzymatic Colorimetry" patented technology

The invention relates to a reagent (kit) for diagnosing/determining amino acid by using an enzyme colorimetric method and an enzyme link method, and also discloses a method for determining the concentration of the amino acid, a composition and components of the reagent, belonging to the technical field of medicine/food/environmental test determination. The reagent (kit) comprises the main components: a buffer solution, a coenzyme, ferricytochrome C, calcium chloride (calcium salt), an amino acid oxidase, a nitrite reductase, a nitrate reductase and a stabilizing agent. A sample and the reagent are mixed according to a certain volume ratio to generate a series of enzymatic reactions, and reactants are placed under an ultraviolet/visible light analyzer for detecting the degree of absorbance rise at the dominant wavelength of 340 nm, thereby measuring and calculating the concentration of the amino acid.

The invention relates to a kit for diagnosing/measuring glycine by utilizing the technologies of the enzymatic doubling amplification method, the enzymic colorimetry and the enzyme linked immunosorbent assay. The invention further relates to a method, a principle and the composition and the components of a reagent for measuring the concentration of the glycine, and belongs to the technical field of medical/food inspection and measurement. The main components of the kit include a buffer solution, coenzyme, a 2-ketoglutaric acid, guanosine monophosphate, glycine aminotransferase, glutamate dehydrogenase, guanosine monophosphate reduced enzyme and a stabilizer. Through mixing a sample and the reagent by a certain volume ratio, a series of enzymatic reactions occur, then the reactant is placed under an ultraviolet/visible light analyzer, and the degree/velocity of the increase in absorbance at 340 nm of the dominant wavelength is detected, thereby measuring the active concentration of the glycine.

The invention relates to a homocysteine diagnosis/determination reagent (kit) using enzyme-colorimetry and enzyme-linked method technology. Meanwhile, the invention also relates to a homocysteine concentration determination method, reagent compositions and components, which belong to the technical filed of medical test and determination. The reagent (kit) of the invention mainly includes: buffer solution, reduced coenzyme, serine, cystathionine Beta synthase, cystathionine Beta catenase, lactic dehydrogenase and stabilizing agent; samples and reagent are mixed according to a certain volume ratio to generate a series of enzymatic reactions; then the reactants are arranged under an ultraviolet/visible light analyzer to detect the decreasing degree of absorbance at the 340nm position of a dominant wave so as to measure and calculate the concentration of the homocysteine.

The invention relates to a kit for diagnosing/measuring potassium (ions) by utilizing the technologies of the enzymic colorimetry and the enzyme linked immunosorbent assay. The invention further relates to a method, a principle and the composition and the components of a reagent for measuring the concentration of the potassium (ions), and belongs to the technical field of medical/food/environmental inspection and measurement. The main components of the kit include a buffer solution, reduced coenzyme, adenosine diphosphate, a phosphoenolpyruvic acid, coenzyme A, pyruvate kinase, pyruvate oxidase, NADH peroxydase and a stabilizer. Through mixing a sample and the reagent by a certain volume ratio, a series of enzymatic reactions occur, then the reactant is placed under an ultraviolet/visible light analyzer, and the velocity of the decrease in absorbance at 340 nm of the dominant wavelength is detected, thereby measuring the concentration of potassium (ions).

The invention relates to a reagent (kit) for measuring formaldehyde by using an enzyme-multiplied method, an enzyme colorimetric method and an enzyme coupling method as well as a method for measuring the concentration of the formaldehyde, and composition and ingredients of the reagent, belonging to the technical field of food/environmental test. The reagent (kit) comprises the main ingredients of buffer solution, coenzyme, acetyl-coenzyme A, 3-formyl acetic acid, formaldehyde dehydrogenase, formic acid C-acetylase, malonic acid-succinate semialdehyde dehydrogenase and stabilizing agent. The concentration of the formaldehyde is measured by mixing a sample and the reagent according to a certain volume ratio to carry out a series of enzymatic reaction, placing the reactant under an ultraviolet/visible light analyzer and detecting the ascending degree of absorbance at a dominant wavelength of 340nm.

The invention relates to a determination method of glycine content by adopting an enzymatic recycling method, an enzymatic colorimetric method and an enzyme-linked immuno sorbent assay technology, and composition and components of a reagent, and the technical principle of the determination is based on serial catalytic reaction of glycine dehydrogenase, glycine oxidase and alanine dehydrogenase. The invention also relates to a kit for determining the glycine. The determination method has the advantages of high sensitivity and small error. Therefore, the determination method and the kit disclosed by the invention can be widely applied to food inspection.

The invention relates to a creatinine diagnostic/determination reagent (kit) adopting an enzymatic colorimetric method and an enzyme coupling method and simultaneously relates to a method for determining concentration of creatinine and components of the reagent, belonging to the technical field of medical test and determination. The reagent (kit) mainly comprises buffer solution, coenzyme, oxidized ferredoxin, creatinine deiminase, ferredoxin nitrite reductase, nitrate reductase and stabilizing agents. The samples and the reagent are mixed according to certain volume ratio and then carry out a series of enzymatic reactions, then the reactants are placed under an ultraviolet/visible light analyzer to test the degree of absorbance rise at the dominant wavelength of 340nm, thus measuring and calculating the concentration of creatinine.

The invention relates to a reagent (kit) for measuring formaldehyde by using an enzyme triple multiplied method, an enzyme colorimetric method and an enzyme coupling method as well as a method for measuring the concentration of the formaldehyde, and composition and ingredients of the reagent, belonging to the technical field of food/environmental test. The reagent (kit) comprises the main ingredients of buffer solution, coenzyme, acetaldehyde, coenzyme A, formaldehyde dehydrogenase, lactic acid aldolase, lactic dehydrogenase, pyruvate dehydrogenase and stabilizing agent. The concentration of the formaldehyde is measured by mixing a sample and the reagent according to a certain volume ratio to carry out a series of enzymatic reaction, placing the reactant under an ultraviolet/visible light analyzer and detecting the ascending degree of absorbance at a dominant wavelength of 340nm.

The invention relates to a reagent (kit) for measuring formaldehyde by using an enzyme-multiplied method, an enzyme colorimetric method and an enzyme coupling method as well as a method for measuring the concentration of the formaldehyde, and composition and ingredients of the reagent, belonging to the technical field of food/environmental test. The reagent (kit) comprises the main ingredients of buffer solution, coenzyme, acetyl-coenzyme A, formaldehyde dehydrogenase, formic acid C-acetylase, coenzyme A-disulfide reducase and stabilizing agent. The concentration of the formaldehyde is measured by mixing a sample and the reagent according to a certain volume ratio to carry out a series of enzymatic reaction, placing the reactant under an ultraviolet/visible light analyzer and detecting the ascending degree of absorbance at a dominant wavelength of 340nm.

The invention relates to a kit for diagnosing/measuring sodium (ions) by utilizing the technologies of the enzymic colorimetry and the enzyme linked immunosorbent assay. The invention further relates to a method, a principle and the composition and the components of a reagent for measuring the concentration of sodium (ions), and belongs to the technical field of medical/food/environmental inspection and measurement. The main components of the kit include a buffer solution, coenzyme, lactose, beta-galactosidase, glucose oxidase, NAD(P)H oxidase and a stabilizer. Through mixing a sample and the reagent by a certain volume ratio, a series of enzymatic reactions occur, then the reactant is placed under an ultraviolet/visible light analyzer, and the degree/velocity of the increase in absorbance at 340 nm of the dominant wavelength is detected, thereby measuring the concentration of sodium (ions).

The invention relates to a kit for diagnosing/measuring lithium by utilizing the technologies of the enzymic colorimetry and the enzyme linked immunosorbent assay, wherein, the activity of the kit can be inhibited by lithium. The invention further relates to a method, a principle and the composition and the components of a reagent for measuring the concentration of lithium, and belongs to the technical field of medical/industrial/environmental inspection and measurement. The main components of the kit include a buffer solution, reduced coenzyme, magnesium chloride, inositol-1-phosphate, an oxaloacetic acid, a pyruvic acid, inositol-1-phosphatase, phosphoenolpyruvate carboxykinase, malic dehydrogenase and a stabilizer. Through mixing a check sample and a lithium sample respectively with the reagent by a certain volume ratio, a series of enzymatic reactions occur, then the reactant is placed under an ultraviolet/visible light analyzer, the degree/velocity of the decrease in absorbance at 340 nm of the dominant wavelength is detected, and the difference in the degree/velocity of the decrease between the check sample and lithium sample is compared, thereby measuring the concentration of lithium.

The invention relates to a homocysteine diagnosing/measuring reagent (kit) using the technologies of an enzyme colorimetric method and an enzyme linkage method. At the same time, the invention also relates to a homocysteine concentration measuring method, the reagent composition and reagent ingredients, which belong to the technical field of medical detection and measurement. The reagent (kit) mainly comprises the following ingredients: a buffer solution, cozymase, cozymase A, oxidized form ferredoxin, catechol, chlorine, homocysteine desulfhydrase, 2-oxobutyryl synthetase, chlorobenzoic acid-1, 2-dioxygenase and stabilizing agents. The method has the following steps: mixing samples and the reagent by the certain volume percent for making the samples and the reagent take a series of enzymatic reaction, and then, placing reactants under an ultraviolet/visible light analyzer to detect the rising degree of the light absorbancy in the position with the main wave length of 340 nm, so the concentration of the homocysteine can be measured and calculated.

The invention relates to a homocysteine diagnosis/determination reagent (kit) using enzyme-colorimetry and enzyme-linked method technology. Meanwhile, the invention also relates to a homocysteine concentration determination method, reagent composition and component, which belongs to the technical filed of medical test and determination. The reagent (box) of the invention mainly includes: buffer solution, coenzyme, 5-methyltetrahydrofolate, methionine synzyme, dihydrofolate reductase and stabilizing agent; samples and reagent are mixed according to a certain volume ratio to generate a series of enzymatic reactions; then the reactants are arranged under an ultraviolet/visible light analyzer to detect the increasing degree of absorbance at the 340nm position of a dominant wave so as to measure and calculate the concentration of the homocysteine.

The present invention relates to the measuring method of the content of glycine, the composition and the component of the reagent using enzyme cycle amplification method, enzyme colorimetric method and enzyme-linked method technology, the technical principle of its measuring is based on hydrogen cyanide synthase, pyruvate synthase, The series of catalyzed reactions of the malonate semialdehyde dehydrogenase is completed, and the invention also relates to a glycine determination kit. The determination method of the invention has high sensitivity and small error, so the determination method and the kit of the invention can be widely used in food inspection.

The present invention relates to the measuring method of the content of ammonia (ammonia ion) using enzyme cycle amplification method, enzyme colorimetric method and enzyme-linked method technology, the composition and the composition of reagent, the technical principle of its measuring is based on glutamic acid oxidase, glutamic acid oxidase, glutamic acid The serial catalytic reactions of alanine aminotransferase and alanine dehydrogenase are completed, and the invention also relates to an ammonia (ammonia ion) diagnosis/measurement kit. The assay method of the present invention has high sensitivity and small error, so the assay method and kit of the present invention can be widely used in clinical medicine/food inspection.

The invention relates to a carbon dioxide diagnosing/measuring reagent box which utilizes the enzyme circulation enlarging method, the enzyme contrasting color method and the enzyme jointing method techniques, and simultaneously the invention also relates to a method principle of the carbon dioxide concentration measuring, and the composition as well as the components of reagent, and belongs to the technical field of the medicine/food/environment test. The main components of the reagent box of the invention include cushion liquid, coenzyme, acetic acid, ferricytochrome b1, coenzyme A, pyruvate dehydrogenase with cytochrom dependency, pyruvate dehydrogenase with coenzyme dependency and stabilizing agent and the reagent box generates a series of enzyme promoting reactions trough the mixing of the samples and the reagent as a certain cubage rate, and then the reactants are positioned under a ultraviolet/visible light analyzer which tests the ascending speed of the absorbency at the main wave length 340nm, thereby measuring the concentration size of the carbon dioxide. The invention can absolutely get needed measuring result through the ultraviolet/visible light analyzer.

The invention relates to a kit for diagnosing/measuring sodium (ions) by utilizing the technologies of the enzymic colorimetry and the enzyme linked immunosorbent assay. The invention further relates to a method, a principle and the composition and the components of a reagent for measuring the concentration of sodium (ions), and belongs to the technical field of medical/food/environmental inspection and measurement. The main components of the kit include a buffer solution, coenzyme, lactose, beta-galactosidase, glucose-6-phosphatase, glucose-6-phosphate dehydrogenase and a stabilizer. Through mixing a sample and the reagent by a certain volume ratio, a series of enzymatic reactions occur, then the reactant is placed under an ultraviolet/visible light analyzer, and the degree/velocity of the increase in absorbance at 340 nm of the dominant wavelength is detected, thereby measuring the concentration of sodium (ions).

The invention relates to a kit for diagnosing/measuring carbon dioxide by utilizing the technologies of the enzymic colorimetry and the enzyme linked immunosorbent assay. The invention further relates to a method, a principle and the composition and the components of a reagent for measuring the concentration of the carbon dioxide, and belongs to the technical field of medical/food/environmental inspection and measurement. The main components of the kit include a buffer solution, reduced coenzyme, a phosphoenolpyruvic acid, phosphoenolpyruvate carboxykinase, malic dehydrogenase and a stabilizer. Through mixing a sample and the reagent by a certain volume ratio, a series of enzymatic reactions occur, then the reactant is placed under an ultraviolet/visible light analyzer, and the degree of the decrease in absorbance at 340 nm of the dominant wavelength is detected, thereby measuring the active concentration of the carbon dioxide.

The invention relates to a saccharose determination kit which uses the techniques of an enzyme colorimetry method and an enzyme-linked method; meanwhile, the invention also relates to a method principle used for determining saccharose consistency, reagent composition and component, belonging to the field of food detection determination technique. The main components of the kit of the invention comprise phosphoric acid buffer solution, reduction-typed coenzyme, sucrose phosphorylase, mannitol dehydrogenase and stabilizer; samples and reagents are mixed according to a certain volume proportion so as to generate a series of enzymatic reactions; subsequently, reaction matters are arranged under an ultraviolet/visible light analyser so as to detect the degree/speed of the absorbency reduction of the main wavelength at the 340nm position, thus calculating the consistency of the saccharose.

The invention belongs to the technical field of biology and particularly relates to an enzymatic chemiluminescence based detection kit for UA (uric acid) determination. UA is oxidized into allantoin and hydrogen peroxide under catalysis of uricase, hydrogen peroxide is subjected to direct reaction with a luminescence substrate, and a persistent high-intensity chemical signal is formed without peroxidase. Compared with the traditional enzymatic colorimetry and chemiluminescence, hydrogen peroxide is subjected to direct reaction with the substrate for luminescence without need of adding catalasefor catalysis, and the method can be applied to full-automatic luminescence instruments and can meet clinical automatic batch detection.

The invention relates to a carbon dioxide diagnosing/testing reagent box which utilizes the enzyme color method, and also relates to a method for testing the consistence of carbon dioxide, and the elements and the components of reagent. The invention belongs to the technical field of the medicine/food/environment check. The reagent box of the invention mainly comprises cushion fluid, pyruvic acid, malate dehydrogenase (codecarboxylase), reduced enzyme and stabilizer. A series of enzyme reactions occur by mixing the sample and the reagent according to a certain volume rate. Then the reactant is arranged under an ultraviolet and visible light analysis instrument to test the reduction extend and the speed of the absorbency at the 340nm of the main wavelength, thereby testing the consistence of the carbon dioxide. By adopting the invention, the testing result can be obtained by the ultraviolet and visible light analysis instrument, and the promotion and the application are convenient.