Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Enzymatic chemiluminescence based detection kit for UA (uric acid) determination

A detection kit and luminescence technology, applied in the biological field, can solve the problems of low specificity, interference and poor stability of peroxidase, and achieve the effects of enhancing anti-interference ability, reducing interference and high sensitivity

Active Publication Date: 2018-06-01
GUANGZHOU JINDE BIOTECH
View PDF5 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this method uses luminol, isoluminol and their derivatives as luminescent substrates, which generally need to add catalysts such as peroxidase. The stability of these reagent components is poor, and the specificity of peroxidase The sensitivity is not high, because hydrogen peroxide is the main intermediate product in this reaction, it is easy to be interfered in the determination process
[0005] Secondly, what cannot be ignored in the process of detecting uric acid at present is the interference caused by endogenous substances in the sample such as hemoglobin, bilirubin and exogenous substances derived from clinical drug applications, such as ascorbic acid, which leads to the detection of uric acid. There is a certain difference between the result and the real value

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Enzymatic chemiluminescence based detection kit for UA (uric acid) determination
  • Enzymatic chemiluminescence based detection kit for UA (uric acid) determination
  • Enzymatic chemiluminescence based detection kit for UA (uric acid) determination

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Embodiment 1, the preparation of Tween 80 primary degradant

[0038] Add Tween 80 solution into a 100ml shake flask, inoculate 1~3wt% Trichoderma viride with a concentration of 1000cfu / mL, set the temperature at 35°C, rotate at 120r / min, cultivate for 12h, centrifuge, and take the supernatant; Microfiltration was performed on clear water, cation exchange and sterilization were performed on 732 type cation exchange resin, and the primary degradation product of Tween 80 was obtained.

Embodiment 2

[0039] Example 2, a detection kit for the determination of uric acid by enzymatic chemiluminescence

[0040] Reagent R1:

[0041]

[0042] Reagent R2:

[0043]

[0044] Reagent R3:

[0045] Lumigen HyPerBlu.

Embodiment 3

[0046] Example 3, a detection kit for the determination of uric acid by enzymatic chemiluminescence

[0047] The difference between Example 3 and Example 2 is that the anti-interference agent I in the reagent R1 is ascorbate oxidase and potassium ferrocyanide, the concentration of the primary degradation product of Tween 80 is 0.03w / v%, and the remaining parameters and operations are as implemented Example 2 shows.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention belongs to the technical field of biology and particularly relates to an enzymatic chemiluminescence based detection kit for UA (uric acid) determination. UA is oxidized into allantoin and hydrogen peroxide under catalysis of uricase, hydrogen peroxide is subjected to direct reaction with a luminescence substrate, and a persistent high-intensity chemical signal is formed without peroxidase. Compared with the traditional enzymatic colorimetry and chemiluminescence, hydrogen peroxide is subjected to direct reaction with the substrate for luminescence without need of adding catalasefor catalysis, and the method can be applied to full-automatic luminescence instruments and can meet clinical automatic batch detection.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a detection kit for measuring uric acid by an enzyme chemiluminescence method. Background technique [0002] The molecular formula of uric acid (2,4,6-trihydroxypurine, referred to as UA) is C 5 h 4 N 4 o 3 , its molecular weight MW=168.11. It is a white crystalline substance that is insoluble in water, acid, ether and ethanol. It is weakly alkaline and can form a salt with strong acid. Uric acid exists in the form of salt in the living body, so it has a high solubility. Uric acid is the end product of purine metabolism in the human body. Most of it is excreted with urine, and a small part is excreted through feces and sweat. Normal value of plasma uric acid: male: 149-416 μmol / L, female: 89-357 μmol / L. When the purine metabolism in the human body is disturbed, the concentration of uric acid in the plasma is too high, which is called hyperuricemia (HUA). The solub...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/53
CPCG01N33/5308G01N2333/902
Inventor 李民友林伟荣张玲蔺涛
Owner GUANGZHOU JINDE BIOTECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com