Creatinine diagnostic reagent (kit) and method for determining concentration of creatinine
A technology for diagnostic reagents and creatinine, which is applied in the field of medical testing and determination, can solve the problems of slowness, reduced accuracy of reagent testing, and inconvenience of testing, etc.
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Embodiment 1
[0041] The creatinine diagnostic reagent of this embodiment is a single reagent, including:
[0042] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L
[0043] Stabilizer 500mmol / L
[0044] Reduced coenzyme 0.25mmol / L
[0045] Creatinine deiminase 6000U / L
[0046] Amino acid dehydrogenase 8000U / L
[0047] Oxygen acid 12mmol / L
[0048] After the reagents are all dissolved and prepared, they are divided into bottles and freeze-dried to make dry powder reagents; before use, add purified water and reconstitute for use.
[0049] Set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, initial absorbance 1.8±0.7, test main wavelength 340nm, test subwavelength 405nm, the volume ratio of the creatinine sample to the reagent to be tested is 1 / 25, The reaction direction is negative reaction (decreasing reaction), the delay time is about 0 minutes, and the detection time is about 5 minutes.
[0050] After adding the samples and reagent...
Embodiment 2
[0052] The creatinine diagnostic reagent of this embodiment is a double reagent, comprising:
[0053] Reagent 1
[0054] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L
[0055] Stabilizer 50mmol / L
[0056] Reduced coenzyme 0.25mmol / L
[0057] Oxygen acid 12mmol / L
[0058] Reagent 2
[0059] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L
[0060] Stabilizer 500mmol / L
[0061] Creatinine deiminase 6000U / L
[0062] Amino acid dehydrogenase 8000U / L
[0063] After the reagents are all dissolved and prepared, they are divided into bottles to make liquid double reagents, which can be used directly.
[0064] Set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, initial absorbance 1.8±0.7, test main wavelength 340nm, test secondary wavelength 405nm, the volume ratio of the creatinine sample to be tested to reagent 1 and reagent 2 is 2 / 20 / 5, the reaction direction is negative reaction (downward reaction), ...
Embodiment 3
[0067] The creatinine diagnostic reagent of this embodiment is three reagents, comprising:
[0068] Reagent 1
[0069] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L
[0070] Stabilizer 50mmol / L
[0071] Reduced coenzyme 0.25mmol / L
[0072] Oxygen acid 12mmol / L
[0073] Reagent 2
[0074] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L
[0075] Stabilizer 500mmol / L
[0076] Amino acid dehydrogenase 8000U / L
[0077] Reagent 3
[0078] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L
[0079] Stabilizer 500mmol / L
[0080] Creatinine deiminase 6000U / L
[0081] After all the reagents are dissolved and prepared, they are divided into bottles to make liquid three reagents, which can be used directly.
[0082] When measuring creatinine concentration, set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, initial absorbance 1.8±0.7, test main wavelength 340nm, test secondary wavelength ...
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