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Creatinine diagnostic reagent (kit) and method for determining concentration of creatinine

A technology for diagnostic reagents and creatinine, which is applied in the field of medical testing and determination, can solve the problems of slowness, reduced accuracy of reagent testing, and inconvenience of testing, etc.

Inactive Publication Date: 2010-06-30
SUZHOU ANJ BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

One of its disadvantages is that while the system generates the reduced nicotinamide coenzyme required for the reaction, it also offsets some of the enzymes such as alanine aminotransferase, aspartate aminotransferase, urea, ammonia, creatinine, and carbon dioxide. activity, causing the accuracy of the reagent test to be greatly reduced
The second disadvantage is that the reagent must be reacted for a period of time before the formal test in order to produce enough reduced nicotinamide coenzyme, which results in slow results and brings great inconvenience to the test

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] The creatinine diagnostic reagent of this embodiment is a single reagent, including:

[0042] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0043] Stabilizer 500mmol / L

[0044] Reduced coenzyme 0.25mmol / L

[0045] Creatinine deiminase 6000U / L

[0046] Amino acid dehydrogenase 8000U / L

[0047] Oxygen acid 12mmol / L

[0048] After the reagents are all dissolved and prepared, they are divided into bottles and freeze-dried to make dry powder reagents; before use, add purified water and reconstitute for use.

[0049] Set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, initial absorbance 1.8±0.7, test main wavelength 340nm, test subwavelength 405nm, the volume ratio of the creatinine sample to the reagent to be tested is 1 / 25, The reaction direction is negative reaction (decreasing reaction), the delay time is about 0 minutes, and the detection time is about 5 minutes.

[0050] After adding the samples and reagent...

Embodiment 2

[0052] The creatinine diagnostic reagent of this embodiment is a double reagent, comprising:

[0053] Reagent 1

[0054] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0055] Stabilizer 50mmol / L

[0056] Reduced coenzyme 0.25mmol / L

[0057] Oxygen acid 12mmol / L

[0058] Reagent 2

[0059] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0060] Stabilizer 500mmol / L

[0061] Creatinine deiminase 6000U / L

[0062] Amino acid dehydrogenase 8000U / L

[0063] After the reagents are all dissolved and prepared, they are divided into bottles to make liquid double reagents, which can be used directly.

[0064] Set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, initial absorbance 1.8±0.7, test main wavelength 340nm, test secondary wavelength 405nm, the volume ratio of the creatinine sample to be tested to reagent 1 and reagent 2 is 2 / 20 / 5, the reaction direction is negative reaction (downward reaction), ...

Embodiment 3

[0067] The creatinine diagnostic reagent of this embodiment is three reagents, comprising:

[0068] Reagent 1

[0069] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0070] Stabilizer 50mmol / L

[0071] Reduced coenzyme 0.25mmol / L

[0072] Oxygen acid 12mmol / L

[0073] Reagent 2

[0074] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0075] Stabilizer 500mmol / L

[0076] Amino acid dehydrogenase 8000U / L

[0077] Reagent 3

[0078] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0079] Stabilizer 500mmol / L

[0080] Creatinine deiminase 6000U / L

[0081] After all the reagents are dissolved and prepared, they are divided into bottles to make liquid three reagents, which can be used directly.

[0082] When measuring creatinine concentration, set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, initial absorbance 1.8±0.7, test main wavelength 340nm, test secondary wavelength ...

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Abstract

The invention relates to a creatinine diagnostic reagent (kit) adopting an enzymatic colorimetric method and an enzyme coupling method and simultaneously relates to a method for determining concentration of creatinine and components of the reagent, belonging to the technical field of medical test and determination. The reagent (kit) mainly comprises buffer solution, reduced coenzyme, oxyacid, creatinine deiminase, amino acid dehydrogenase and stabilizing agents. The samples and the reagent are mixed according to certain volume ratio and then carry out a series of enzymatic reactions, then the reactants are placed under an ultraviolet / visible light analyzer to test the degree of absorbance decline at the dominant wavelength of 340nm, thus measuring and calculating the concentration of creatinine.

Description

technical field [0001] The invention relates to a creatinine diagnostic reagent (box), and at the same time, the invention also relates to a method for measuring creatinine concentration, which belongs to the technical field of medical examination and measurement. Background technique [0002] Determination of creatinine mainly includes chemical determination method (Jaffe method), enzymatic method, high performance liquid chromatography and capillary electrophoresis. [0003] The chemical determination method is low in cost and easy to operate, and is one of the most commonly used methods for the determination of creatinine in China. The creatinine in the specimen interacts with picrate to form a yellow-red complex of picric acid and creatinine. The disadvantage of this method is that the specificity is not high, and vitamin C, acetone, acetoacetic acid, methyldopa, high concentration of glucose, protein and some antibiotics such as penicillin G, cefoxitin, cefazolin, etc....

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/31G01N21/17G01N33/48C12Q1/26
Inventor 王尔中
Owner SUZHOU ANJ BIOTECHNOLOGY CO LTD
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