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Creatinine diagnostic/determination reagent (kit) and method for determining concentration of creatinine

A technology for concentration determination and reagents, which is applied in the field of medical testing and determination, and can solve the problems of lower testing accuracy of reagents, inconvenient testing, and urgent problems.

Inactive Publication Date: 2010-06-30
SUZHOU ANJ BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

One of its disadvantages is that while the system generates the reduced nicotinamide coenzyme required for the reaction, it also offsets some of the enzymes such as alanine aminotransferase, aspartate aminotransferase, urea, ammonia, creatinine, and carbon dioxide. activity, causing the accuracy of the reagent test to be greatly reduced
The second disadvantage is that the reagent must be reacted for a period of time before the formal test in order to produce enough reduced nicotinamide coenzyme, which results in slow results and brings great inconvenience to the test

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] The creatinine diagnostic / measurement reagent in this embodiment is a single reagent, including:

[0045] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0046] Stabilizer 500mmol / L

[0047] Coenzyme 3mmol / L

[0048] Creatinine deiminase 10000U / L

[0049] Ferredoxin nitrite reductase 12000U / L

[0050] Nitrate reductase 12000U / L

[0051] Oxidized ferredoxin 12mmol / L

[0052] After all the reagents are dissolved and prepared, they are divided into bottles and freeze-dried to prepare dry powder reagents; before use, add pure water and use after reconstitution.

[0053] Set on the automatic biochemical analyzer: temperature 37℃, reaction time 10 minutes, initial absorbance ≤0.1, test main wavelength 340nm, test sub-wavelength 405nm, the volume ratio of tested creatinine sample to reagent is 1 / 25, reaction The direction is positive reaction (rising reaction), the delay time is about 0 minutes, and the detection time is about 5 minutes.

[0054] After adding the sample...

Embodiment 2

[0056] The creatinine diagnostic / measurement reagent in this embodiment is a dual reagent, including:

[0057] Reagent 1

[0058] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0059] Stabilizer 50mmol / L

[0060] Coenzyme 3mmol / L

[0061] Oxidized ferredoxin 12mmol / L

[0062] Reagent 2

[0063] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0064] Stabilizer 50mmol / L

[0065] Creatinine deiminase 10000U / L

[0066] Ferredoxin nitrite reductase 12000U / L

[0067] Nitrate reductase 12000U / L

[0068] After all the reagents are dissolved and prepared, they are divided into bottles to make a liquid double reagent, which can be used directly.

[0069] Set on the automatic biochemical analyzer: temperature 37℃, reaction time 10 minutes, initial absorbance ≤0.1, test main wavelength 340nm, test sub-wavelength 405nm, the volume ratio of the tested creatinine sample to reagent 1 and reagent 2 is 2 / 20 / 5, the reaction direction is positive reaction (rising reacti...

Embodiment 3

[0072] The creatinine diagnosis / determination reagent in this embodiment is three reagents, including:

[0073] Reagent 1

[0074] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0075] Stabilizer 50mmol / L

[0076] Coenzyme 3mmol / L

[0077] Oxidized ferredoxin 12mmol / L

[0078] Reagent 2

[0079] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0080] Stabilizer 500mmol / L

[0081] Ferredoxin nitrite reductase 12000U / L

[0082] Nitrate reductase 12000U / L

[0083] Reagent 3

[0084] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0085] Stabilizer 500mmol / L

[0086] Creatinine deiminase 10000U / L

[0087] After all the reagents are dissolved and prepared, they are divided into bottles to make three liquid reagents, which can be used directly.

[0088] When determining the creatinine concentration, set on the automatic biochemical analyzer: temperature 37℃, reaction time 10 minutes, initial absorbance ≤0.1, test main wavelength 340nm, test ...

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Abstract

The invention relates to a creatinine diagnostic / determination reagent (kit) adopting an enzymatic colorimetric method and an enzyme coupling method and simultaneously relates to a method for determining concentration of creatinine and components of the reagent, belonging to the technical field of medical test and determination. The reagent (kit) mainly comprises buffer solution, coenzyme, oxidized ferredoxin, creatinine deiminase, ferredoxin nitrite reductase, nitrate reductase and stabilizing agents. The samples and the reagent are mixed according to certain volume ratio and then carry out a series of enzymatic reactions, then the reactants are placed under an ultraviolet / visible light analyzer to test the degree of absorbance rise at the dominant wavelength of 340nm, thus measuring and calculating the concentration of creatinine.

Description

Technical field [0001] The present invention relates to a creatinine diagnosis / determination reagent (kit). At the same time, the present invention also relates to a method for determining the concentration of creatinine, which belongs to the technical field of medical testing and determination. Background technique [0002] The determination of creatinine mainly includes chemical determination (Jaffe method), enzymatic method, high performance liquid chromatography and capillary electrophoresis. [0003] The chemical determination method is cheap and easy to operate. It is currently one of the most commonly used methods for determining creatinine in China. The creatinine in the specimen reacts with picrate to form a yellow-red picric creatinine complex. The disadvantage of this method is that the specificity is not high. Vitamin C, acetone, acetoacetic acid, methyldopa, high concentrations of glucose, protein and some antibiotics such as penicillin G, cefoxitin, cefazolin, etc. c...

Claims

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Application Information

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IPC IPC(8): G01N21/31G01N21/17G01N33/48C12Q1/26
Inventor 王尔中
Owner SUZHOU ANJ BIOTECHNOLOGY CO LTD
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