Creatinine diagnostic/determination reagent (kit) and method for determining concentration of creatinine

A technology for concentration determination and reagents, which is applied in the field of medical testing and determination, and can solve the problems of lower testing accuracy of reagents, inconvenient testing, and urgent problems.
CN101762497AInactive Publication Date: 2010-06-30SUZHOU ANJ BIOTECHNOLOGY CO LTD
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Patent Information

Authority / Receiving Office
CN Β· China
Current Assignee / Owner
SUZHOU ANJ BIOTECHNOLOGY CO LTD
Publication Date
2010-06-30
Estimated Expiration
Not applicable Β· inactive patent
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Abstract

The invention relates to a creatinine diagnostic / determination reagent (kit) adopting an enzymatic colorimetric method and an enzyme coupling method and simultaneously relates to a method for determining concentration of creatinine and components of the reagent, belonging to the technical field of medical test and determination. The reagent (kit) mainly comprises buffer solution, coenzyme, oxidized ferredoxin, creatinine deiminase, ferredoxin nitrite reductase, nitrate reductase and stabilizing agents. The samples and the reagent are mixed according to certain volume ratio and then carry out a series of enzymatic reactions, then the reactants are placed under an ultraviolet / visible light analyzer to test the degree of absorbance rise at the dominant wavelength of 340nm, thus measuring and calculating the concentration of creatinine.
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Description

Technical field

[0001] The present invention relates to a creatinine diagnosis / determination reagent (kit). At the same time, the present invention also relates to a method for determining the concentration of creatinine, which belongs to the technical field of medical testing and determination. Background technique

[0002] The determination of creatinine mainly includes chemical determination (Jaffe method), enzymatic method, high performance liquid chromatography and capillary electrophoresis.

[0003] The chemical determination method is cheap and easy to operate. It is currently one of the most commonly used methods for determining creatinine in China. The creatinine in the specimen reacts with picrate to form a yellow-red picric creatinine complex. The disadvantage of this method is that the specificity is not high. Vitamin C, acetone, acetoacetic acid, methyldopa, high concentrations of glucose, protein and some antibiotics such as penicillin G, cefoxitin, cefazolin, etc. c...

Claims

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