6 results about "Nitrite reductase" patented technology

The application relates to a nitrite reductase electrode and a preparation method thereof and an electrochemical system. The nitrite reductase electrode comprises a substrate layer and n electron transfer layers and n enzyme activity layers in the form of alternating layers above the substrate layer, n is in the range of 2-30, wherein the substrate layer comprises a carbon-based electrode loaded with a transition metal phosphide, and the enzyme activity layer comprises a nitrite reductase. The nitrite reductase electrode has low detection limit, high sensitivity, high enzyme loading rate, high electrocatalytic efficiency and good application prospect.

The application belongs to the field of enzyme engineering, and relates to degradation of aflatoxin B1 and zearalenone. The application provides application of nitrite reductase AfNiR in simultaneous degradation of aflatoxin B1 and zearalenone. The nitrite reductase AfNiR has an optimal temperature of 40 DEG C and an optimal pH of 7.0 for degradation of AFB1. The degradation rate of the enzyme to AFB1 can reach 92.92%. Meanwhile, the nitrite reductase AfNiR can maintain a degradation rate of AFB1 of more than 80% in a temperature range of 40-70 DEG C, and is a fungal toxin degradation enzyme that can tolerate a certain degree of high temperature. The application provides a candidate enzyme with application potential for detoxification of AFB1 and ZEN in the food and feed industries.

The present application provides a kind of marine bacteria capable of degrading lignin and removing nitrite, which is a deep-sea seabed sulfide source, and has the function of degrading lignin and removing nitrite.The strain of Halomonas sp.has a preservation number of CGMCC No.34690.The strain screened in the present application can grow and reproduce in seawater, can utilize guaiacol as a carbon source, and can remove nitrite.The strain of the present application has laccase activity, has laccase gene and nitrite reductase gene.The experimental results show that the cumulative removal rate of guaiacol after 2 days of culture is 56%, and the removal rate of nitrite after 4 days of culture is close to 70%, which has great application potential in the marine aquaculture industry.

This invention belongs to the field of aquaculture, specifically relating to a composite nitrite degrading agent for aquaculture water and its preparation method. The method includes the following steps: modifying nano-iron with *Violetella foenum-graecum* extract; preparing the nitrite microecological degrading agent; and preparing the composite nitrite degrading agent. This invention uses a compound of *Bacillus belye*, *Glutamic acid bacillus mysore*, and *Enterococcus faecium* to construct the microecological degrading agent. Through the synergistic effect of nitrite reductase, the yield and specific activity of nitrite reductase are significantly improved, resulting in a nitrite degradation rate of much higher than that of a single-strain fermentation system, achieving highly efficient nitrite degradation.

The present application relates to a kind of nitrite reductase mutant and its application, the amino acid sequence of the nitrite reductase mutant relative to wild-type nitrite reductase, with the substitution of amino acid in one or more positions corresponding to the 48th, 64th, 70th and 240th of SEQ ID NO:1.The nitrite reductase mutant provided by the present application has increased stability compared with wild-type nitrite reductase, and can be applied to degrade nitrite in sewage.The preparation method of the nitrite reductase mutant provided by the present application has simple process and mild conditions, and has good application prospect.

The invention relates to the technical field of plant physiological regulation and biological enzyme preparations, in particular to a compound enzyme preparation for improving / solving black explosion smoke as well as a preparation method and application of the compound enzyme preparation. Comprising the following components in parts by mass: 5-20 parts of an amino acid compound, 5-15 parts of an organic potassium source, 10-20 parts of potassium fulvate, 3-15 parts of an active small molecule synergist, 1-5 parts of an amino acid metabolic enzyme, 1-5 parts of nitrate reductase, 0.5-3 parts of nitrite reductase and 1-10 parts of an auxiliary agent. According to the method, by introducing exogenous nitrate reductase and nitrite reductase, an internal denitrification metabolic pathway of tobacco cells is directly enhanced, and accumulated nitrate nitrogen is quickly converted into available amino nitrogen. According to the process, the nitrate nitrogen content is reduced by 60%, the formation substrate of the black exploded tobacco is greatly reduced from the source, the occurrence rate of the black exploded tobacco is reduced by 80% or above, the formation of the black exploded tobacco can be remarkably reduced, and the color, luster and aroma quality of the tobacco leaves are improved.