12 results about "Nitrite reductase" patented technology

The application relates to a nitrite reductase electrode and a preparation method thereof and an electrochemical system. The nitrite reductase electrode comprises a substrate layer and n electron transfer layers and n enzyme activity layers in the form of alternating layers above the substrate layer, n is in the range of 2-30, wherein the substrate layer comprises a carbon-based electrode loaded with a transition metal phosphide, and the enzyme activity layer comprises a nitrite reductase. The nitrite reductase electrode has low detection limit, high sensitivity, high enzyme loading rate, high electrocatalytic efficiency and good application prospect.

The application belongs to the field of enzyme engineering, and relates to degradation of aflatoxin B1 and zearalenone. The application provides application of nitrite reductase AfNiR in simultaneous degradation of aflatoxin B1 and zearalenone. The nitrite reductase AfNiR has an optimal temperature of 40 DEG C and an optimal pH of 7.0 for degradation of AFB1. The degradation rate of the enzyme to AFB1 can reach 92.92%. Meanwhile, the nitrite reductase AfNiR can maintain a degradation rate of AFB1 of more than 80% in a temperature range of 40-70 DEG C, and is a fungal toxin degradation enzyme that can tolerate a certain degree of high temperature. The application provides a candidate enzyme with application potential for detoxification of AFB1 and ZEN in the food and feed industries.

The present application provides a kind of marine bacteria capable of degrading lignin and removing nitrite, which is a deep-sea seabed sulfide source, and has the function of degrading lignin and removing nitrite.The strain of Halomonas sp.has a preservation number of CGMCC No.34690.The strain screened in the present application can grow and reproduce in seawater, can utilize guaiacol as a carbon source, and can remove nitrite.The strain of the present application has laccase activity, has laccase gene and nitrite reductase gene.The experimental results show that the cumulative removal rate of guaiacol after 2 days of culture is 56%, and the removal rate of nitrite after 4 days of culture is close to 70%, which has great application potential in the marine aquaculture industry.

The invention discloses a gene editing method for regulating and controlling the nitrogen utilization rate of rice and a regenerated rice plant, and relates to the technical field of agricultural biology. The gene editing method for regulating and controlling the nitrogen utilization rate of the rice comprises the following steps: selecting a key gene of nitrogen metabolism of the rice as a target gene; determining a target spot in a promoter region of the target gene; constructing a repair donor template containing a nitrate response element based on the sequences on the two sides of the target spot; wherein the nitrate response element is derived from a promoter region of rice nitrite reductase, and can respond to a nitrate signal to enhance the expression of a downstream gene; jointly delivering the repaired donor template and a CRISPR / Cas9 carrier to the rice callus through gene editing, and culturing; after the culture is completed, screening to obtain a regenerated rice plant with the nitrate response element accurately inserted in the genome. According to the application, the nitrate response element is inserted into the promoter region of the nitrogen metabolism key gene to activate gene expression induced by nitrate, so that nitrogen utilization of rice is enhanced, and regenerated rice plants with high nitrogen utilization efficiency are obtained.

The application belongs to the technical field of microorganisms, and particularly discloses a bacillus velezensis DZ-4-002 and application thereof. Bacillus velezensis The bacillus velezensis DZ-4-002 provided by the application has a Latin name of CGMCC No. 25955. The strain can not only produce a high yield of nitrite reductase, but also improve the growth performance of animals and the milk production performance of dairy cows. Within the experimental range of the application, the bacillus velezensis provided by the application can not only improve the egg production increase rate, average egg weight and feed utilization rate of laying hens, but also improve the average daily weight gain, average daily feed intake and feed utilization rate of weaned piglets, and the average milk yield, milk-feed ratio and lactose content in milk of dairy cows. The application has a good application prospect in the fields of nitrite degradation agents, feed and additives thereof.

This invention belongs to the field of aquaculture, specifically relating to a composite nitrite degrading agent for aquaculture water and its preparation method. The method includes the following steps: modifying nano-iron with *Violetella foenum-graecum* extract; preparing the nitrite microecological degrading agent; and preparing the composite nitrite degrading agent. This invention uses a compound of *Bacillus belye*, *Glutamic acid bacillus mysore*, and *Enterococcus faecium* to construct the microecological degrading agent. Through the synergistic effect of nitrite reductase, the yield and specific activity of nitrite reductase are significantly improved, resulting in a nitrite degradation rate of much higher than that of a single-strain fermentation system, achieving highly efficient nitrite degradation.

The invention discloses an application of a gene OsbZIP11 in improving the low nitrogen stress tolerance of rice. According to the invention, an OsbZIP11 gene knockout plant bzip11-1 and bzip11-2 is prepared by utilizing a CRISPR / Cas9 gene editing technology, a mechanism for enhancing the low-nitrogen stress tolerance of rice seedlings by the OsbZIP11 is disclosed, nitrogen absorption is increased by regulating and controlling NO3 <-> acquisition rate and root activity, and meanwhile, by improving the activity of key enzymes such as nitrate reductase (NR), nitrite reductase (NiR) and glutamate synthase (GOGAT), the low-nitrogen stress tolerance of the rice seedlings is enhanced. The plant nitrogen assimilation capability is enhanced, and finally the low nitrogen stress tolerance capability of the rice is enhanced. The invention provides a high-quality candidate gene and a theoretical basis for cultivating low-nitrogen nutrition tolerant rice varieties.

The present application relates to a kind of nitrite reductase mutant and its application, the amino acid sequence of the nitrite reductase mutant relative to wild-type nitrite reductase, with the substitution of amino acid in one or more positions corresponding to the 48th, 64th, 70th and 240th of SEQ ID NO:1.The nitrite reductase mutant provided by the present application has increased stability compared with wild-type nitrite reductase, and can be applied to degrade nitrite in sewage.The preparation method of the nitrite reductase mutant provided by the present application has simple process and mild conditions, and has good application prospect.

The invention provides a marine bacterium capable of degrading lignin and removing nitrite, which is a halomonas strain which is derived from deep sea bottom sulfide and has the functions of degrading lignin and removing nitrite, and the preservation number of the marine bacterium is CGMCC No.34690. The screened strain can grow and breed in seawater, guaiacol can be used as a carbon source, and nitrite can be removed. The strain provided by the invention has laccase activity, a laccase gene and a nitrite reductase gene. Experimental results show that the removal rate of guaiacol reaches 56% after culture for 2 days, the removal rate of nitrate is close to 70% after culture for 4 days, and the strain has great application potential in the mariculture industry.

A method for rapidly achieving dual-short-range coupled anaerobic ammonium oxidation using domestic sewage as a carbon source through anaerobic / aerobic alternating starvation, belonging to the field of sewage biological treatment. 1. Close the inlet and outlet water systems, continue stirring and intermittent aeration, and maintain the anaerobic / aerobic starvation state; 2. Starve for 10 to 20 days; 3. Restore sludge activity; 4. Start dual-short-range coupled anaerobic ammonium oxidation. This method inhibits the activity of nitrite oxidizing bacteria and the expression level of the nitrite reductase functional gene through starvation treatment, while ammonia oxidizing bacteria (AOB) respond quickly after the influent is restored, and the expression of the nitrate reductase functional gene is not affected. In the short term, the organic matter in domestic sewage is used as a carbon source to achieve dual short-range, control the residual ammonia nitrogen and sludge age, maintain the stable operation of the dual short-range, and provide a stable substrate source for the subsequent coupled anaerobic ammonium oxidation reaction. The present invention is simple to operate and low in cost, and can quickly achieve dual short-range and maintain a high nitrite accumulation rate for a long time.

The invention relates to the technical field of plant physiological regulation and biological enzyme preparations, in particular to a compound enzyme preparation for improving / solving black explosion smoke as well as a preparation method and application of the compound enzyme preparation. Comprising the following components in parts by mass: 5-20 parts of an amino acid compound, 5-15 parts of an organic potassium source, 10-20 parts of potassium fulvate, 3-15 parts of an active small molecule synergist, 1-5 parts of an amino acid metabolic enzyme, 1-5 parts of nitrate reductase, 0.5-3 parts of nitrite reductase and 1-10 parts of an auxiliary agent. According to the method, by introducing exogenous nitrate reductase and nitrite reductase, an internal denitrification metabolic pathway of tobacco cells is directly enhanced, and accumulated nitrate nitrogen is quickly converted into available amino nitrogen. According to the process, the nitrate nitrogen content is reduced by 60%, the formation substrate of the black exploded tobacco is greatly reduced from the source, the occurrence rate of the black exploded tobacco is reduced by 80% or above, the formation of the black exploded tobacco can be remarkably reduced, and the color, luster and aroma quality of the tobacco leaves are improved.

The invention discloses L-alanine composite powder capable of eliminating nitrite and a preparation method of the L-alanine composite powder. The L-alanine composite powder is prepared from ferrous chloride, calcium chloride, sodium alginate, L-alanine, gamma-aminobutyric acid, L-glutamic acid, cysteine, nitrite reductase, trehalose, calcium citrate and ascorbic acid. The preparation method comprises the following steps: preparing an amino acid mixed solution, embedding sodium alginate gel, preparing microspheres by an ionic cross-linking method, freeze-drying and crushing. The Fe < 2 + > / Ca < 2 + > bi-crosslinking gel is utilized to realize the balance between slow release and mechanical strength; a cysteine-nitrite reductase-Fe < 2 + > ternary system is constructed, nitrite can be synergistically catalyzed to generate nitric oxide, and the cysteine-nitrite reductase-Fe < 2 + > ternary system has the functions of removing nitrite and inhibiting production of nitrosamine; the obtained L-alanine composite powder has the capabilities of rapidly eliminating nitrite, slowly releasing nitric oxide, inhibiting generation of new nitrosamine and capturing existing nitrosamine.