20 results about "Cloned genes" patented technology

The application discloses application of a SdC2'H gene of Saposhnikovia divaricata and a method for increasing coumarin content in Saposhnikovia divaricata, and belongs to the technical field of bio-agriculture. The SdC2'H gene of Saposhnikovia divaricata and the biological material containing the SdC2'H gene are applied to the regulation of coumarin synthesis in Saposhnikovia divaricata. The method for increasing coumarin content in Saposhnikovia divaricata comprises the steps of increasing the expression amount and / or activity of the SdC2'H gene in Saposhnikovia divaricata. The SdC2'H gene is successfully cloned from Saposhnikovia divaricata for the first time, and a gene overexpression vector is constructed. The SdC2'H gene is positively regulated to control the synthesis of coumarin for the first time, and overexpression of the SdC2'H gene can significantly increase the product content. This not only deepens the understanding of the molecular mechanism of related biosynthesis, but also lays an important foundation for efficient production of high-value coumarin by using genetic engineering technology.

The invention discloses wheat TaPAB1 protein and application of related biological materials of the wheat TaPAB1 protein in the aspect of improving the grain weight of wheat. A gene for coding the TaPAB1 protein is named as a TaPAB1 gene. According to the invention, the gene TaPAB1 is cloned from a wheat cultivation variety, Chinese spring, and is over-expressed in a wheat variety Kenong 199 (KN199) by utilizing a gene engineering technology, so that the thousand seed weight, the grain length, the plant height, the ear length and the yield of a TaPAB1 over-expressed plant are obviously increased compared with those of a wild type. The invention provides a research basis for increasing the wheat yield and breeding high-yield varieties.

This invention discloses the application of the CLE11 small peptide gene in regulating plant salt tolerance, belonging to the field of biomaterials technology. This invention uses millet as the research material and clones... SiPROCLE11 Gene (CLE11 small peptide gene), construction SiPROCLE11 Overexpression and silencing of millet materials, exogenous application of the synthetic peptide SiCLE11p, and its role in enhancing millet salt tolerance were determined. Further investigation revealed that it can promote lateral root development and reduce Na+ in the roots. + This accumulation of knowledge laid the foundation for the discovery of salt-tolerant genes in millet and for salt-tolerant millet breeding.

The application discloses C4 corn ZmClpP6 protein and application of related biological materials thereof in regulation of high light response capability of plants. ZmClpP6 It is found that: ZmClpP6 The knockout can reduce chlorophyll content, total carotenoid content, catalase activity and copper-zinc SOD enzyme activity of corn under high light conditions, and makes corn leaves appear yellowing phenotype. It is indicated that ZmClpP6 can regulate high light response capability of plants. The application clones ZmClpP6 the gene in corn for the first time and analyzes the biological function thereof, and has important significance for cultivation of high light efficiency plants.

This invention provides rice OsPDRA3 Application of genes in improving insect resistance in rice. This invention clones genes from the rice genome. OsPDRA3 Gene (LOC_Os01g42380), analyzed using the SMART program, predicts that this protein possesses multiple transmembrane domains and ATPase domains (AAA+) associated with various cellular activities. Further investigation is needed. OsPDRA3 The effect on tolerance of rice brown planthopper was investigated by comparing wild-type and overexpression. OsPDRA3 The tolerance of transgenic plants to brown planthoppers was assessed, and the results showed that overexpression of [the planthopper species]... OsPDRA3 Compared to wild-type rice lines (0% survival rate), the survival rate of rice lines treated with brown planthoppers was significantly improved, reaching 75%–100%. This indicates that… OsPDRA3 Genes can significantly improve the insect resistance of rice and can be widely used in the fields of plant genetic engineering and plant disease resistance, with huge economic value and application prospects.

The application belongs to the field of biological genetic engineering and relates to application of PgMYC3 in regulation of ginsenoside biosynthesis. The sequence of the ginseng transcription factor PgMYC3 is shown as SEQ ID NO. 2. The application clones the PgMYC3 gene, constructs a plant expression vector, and adopts the Agrobacterium-mediated transformation method to transform ginseng callus. Through RT-qPCR technology identification, positive callus overexpressing PgMYC3 is obtained. In the transgenic ginseng callus, the total saponin content is increased by 1.5 times, which indicates that PgMYC3 has a promoting effect on the accumulation of ginsenosides. Meanwhile, through molecular biology technology, it is confirmed that PgMYC3 can directly bind to the G-box element to activate the expression of the saponin synthesis gene PgPPDS.

The application belongs to the technical field of genetic engineering, and particularly relates to a CsECT protein related to the disease resistance of plants, a recombinant vector and application thereof. 6 The CsECT protein provided by the application is synthesized by the gene CsECT, and the gene CsECT is speculated to be an m A reader, which affects the metabolism of target mRNAs, improves the stability of disease resistance related target genes, promotes the expression of disease resistance genes, and further improves the disease resistance of plants. The results of the examples show that the CsECT gene is cloned to construct a CsECT transgenic citrus, the transgenic citrus overexpresses the CsECT gene, and the disease resistance of the citrus to bacterial wilt can be significantly improved, thereby improving the disease resistance of the citrus. It can be seen that the CsECT protein synthesized by the gene CsECT can improve the disease resistance of plants.

This invention provides GhFRC1 The application of genes in regulating plant fertility belongs to the field of plant genetic engineering technology. This invention is the first to clone genes from cotton. GhFRC1 The gene was identified and verified to be heterologously expressed in three different genera and families of crops: Arabidopsis thaliana, tomato, and rice, and to play a role in fertility regulation. This breakthrough overcomes the limitation of existing fertility genes that can only regulate the fertility of a single crop, and realizes "broad-spectrum regulation of fertility of multiple crops by a single gene", filling the gap in the application of distant heterologous expression of cotton fertility genes. GhFRC1 After heterologous expression of the gene, it can stably lead to inactive pollen and abnormal fruit set in the recipient crop, and can also cause a phenotype of complete pollen sac non-development. Moreover, the sterility trait can be restored to fertility through normal pollen pollination. This solves the problem of the narrow applicability of existing fertility genes and the great limitation of their breeding applications, and provides new gene resources and technical solutions for fertility regulation of multiple crops and hybridization breeding.

The present application relates to the field of agricultural biotechnology, in particular to locust immune related protein LmEaster gene and application thereof. The present application clones LmEaster gene and synthesizes double-stranded RNA, adopts injection method to treat locusts, and detects the mortality of locusts in each treatment and the expression amount change of related antibacterial peptide, and confirms that after interfering with LmEaster gene of locusts, the expression amount of antibacterial peptide of locusts is increased, the immune defense ability of locusts is increased, and then the mortality is reduced.

The invention relates to the technical field of plant molecular biology, in particular to application of a Unicaulis gene for regulating and controlling growth of tomato axillary buds in tomato breeding. The Uniicaures gene is used for positively regulating and controlling the elongation and growth of lateral branches of tomatoes, and the nucleotide sequence of the Uniicaures gene is as shown in SEQ ID NO. 1; the amino acid sequence of the encoding protein of the Unicaulis gene is as shown in SEQ ID NO. 2. The tomato lateral branch elongation growth gene Unicaulis is positively regulated and controlled by constructing a genetic linkage group, finely positioning and cloning, and by overexpressing the Unicaulis gene in a tomato lateral branch-free plant or increasing the content of the encoding protein of the Unicaulis gene, the accumulation amount of auxin at the axilla part of a transgenic plant is reduced, and the lateral branch of the tomato can normally sprout and obviously grow. The Unicaulis gene or the encoded protein thereof provided by the invention can significantly reduce the accumulation amount of auxin in the axillary part of the tomato plant, relieve the apical dominance of the tomato plant to lateral branches, accelerate the extension and growth of axillary buds of the axillary part of the tomato plant into lateral branches, and provide gene resources and new germplasm for cultivating tomato varieties with strong lateral branches.

The application discloses a method for analyzing epigenetic diversity based on a methylation-sensitive amplified polymorphism (MSAP) gene and a biological gene fingerprint table. The method comprises the following steps: obtaining an MSAP amplification electropherogram of a biological sample to be detected, and constructing a 01 matrix of the sample; converting the 01 matrix into an MSAP gene sequence by using a "CCGGN" replacement method; comparing the MSAP gene sequence with a reference genome, and annotating DNA methylation related gene information; based on the annotation result, constructing a gene fingerprint table based on the copy number of the methylation gene and the methylation / hemimethylation mode, and analyzing epigenetic diversity. The method can convert a traditional MSAP band fingerprint map into a gene fingerprint table with annotation function information without performing cloning and sequencing of MSAP differential fragments, can improve MSAP marker analysis to epigenome analysis, solves the problem that MSAP epigenes are difficult to clone, and improves the level of germplasm resource identification and molecular breeding.

The invention relates to the technical field of biology, in particular to application of ZmATG13a protein and a coding gene thereof to regulation and control of corn yield, ZmATG13a gene is cloned in corn, CRISPR / Cas9 mutant plants of corn ZmATG13a are obtained through genetic transformation, corn selfing line groups are sown in a sufficient phosphorus land parcel, and it is found that the zmatg13a mutant plants have higher plant height than wild type spinning periods, so that the ZmATG13a protein can be used for regulating and controlling corn yield. The stem diameter is not obviously different from that of a wild type; the ear length of the zmatg13a mutant plant is obviously longer than that of a wild type plant, and the single ear weight, the ear grain weight and the hundred-grain weight of the zmatg13a mutant plant are all larger than those of the wild type plant, so that the zmatg13a mutant plant shows the advantage in yield. The ZmATG13a is a protein with an ATG13 structural domain, and the ZmATG13 gene is knocked out through a gene editing method, so that the plant height and the yield in the spinning period are remarkably increased, and a germplasm resource is provided for cultivating a new variety.

The invention provides application of a rice OsPDRA3 gene in improving insect resistance of rice. According to the invention, an OsPDRA3 gene (LOCOs01g42380) is cloned in a rice genome, and an SMART program is used for analyzing and predicting that the protein has multiple transmembrane domains and ATP enzyme structural domains (AAA < + >) related to various cell activities. The influence of OsPDRA3 on the tolerance of rice brown planthopper is further studied, and by comparing the tolerance of wild type and overexpressed OsPDRA3 transgenic plants to brown planthopper, the result shows that the survival rate of a rice line with overexpressed OsPDRA3 after being treated by brown planthopper is obviously increased compared with that of a wild type rice line (the survival rate is 0%), and reaches 75%-100%. Namely, it is shown that the OsPDRA3 gene can significantly improve the insect resistance of rice, can be widely applied to the fields of plant genetic engineering and plant disease resistance, and has huge economic value and application prospects.

A method of erasing an abnormal epigenetic modification in a sheep iCHI embryo and use thereof. A method including the steps of: transferring a recombinant plasmid expressing protamine into a sheep androgenetic haploid embryonic stem cell, then introducing the cell into a mature sheep oocyte, and performing activation and culture to obtain a sheep iCHI embryo without abnormal epigenetic modifications. In the present disclosure, abnormal methylations in iCHI embryos may be erased by transient expression of protamine in o-haSCs. The blastocyst rate of the prepared Pro-iCHI embryos is significantly higher than that of iCHI embryos, and is similar to that of IVF embryos. The resulting embryos can be used for embryo transfer to obtain semi-cloned gene-edited sheep, providing materials for subsequent breeding applications.

The invention relates to a zpap gene and application thereof in regulation and control of the form of bacillus subtillis, and aims at the technical problem that a key genetic target for mediating opaque and semitransparent colony form conversion of the bacillus subtillis lacks in the prior art, the zpap gene (SEQ ID NO.1) is cloned, and a recombinant expression vector of the zpap gene is constructed and converted into the bacillus subtillis. The key technical means is to specifically induce the colonial morphology to be converted from opaque to semitransparent by up-regulating the expression of the zpap gene, and confirm that the process is related to the regulation of the integrity of the cell wall. According to the invention, the blank of a cell wall active genetic regulation mechanism is filled, and a new key target and an effective strategy are provided for performance improvement of industrial strains.

The invention discloses a wheat TaHOT5 protein, a TaHOT5 gene and application of the TaHOT5 protein and the TaHOT5 gene in regulation and control of heat resistance of wheat. The TaHOT5 protein is derived from wheat, and the gene for coding the TaHOT5 protein is named as the TaHOT5 gene. Through a gene engineering technology, the gene TaHOT5 is cloned from a wheat cultivation variety 'Chinese spring', an overexpression vector is constructed, and overexpression of the gene TaHOT5 is realized in a wheat variety Fielder. Compared with a wild type, a TaHOT5 overexpression plant shows remarkably enhanced heat resistance, and the regulation effect of the TaHOT5 gene on the heat resistance of wheat is proved. The invention discloses a key function of the TaHOT5 gene in wheat heat resistance formation, provides a new molecular target for plant heat resistance mechanism research, provides new gene resources and technical approaches for high-temperature-resistant wheat germplasm creation and variety breeding, and has important theoretical value and application prospect.

The invention discloses DNA demethylase IbROS1 related to sweet potato root tuber development and application, and particularly relates to the field of plant genetic engineering and molecular breeding, the nucleotide sequence of the IbROS1 gene is as shown in SEQ ID No.1, and the sequence of protein coded by the IbROS1 gene is as shown in SEQ ID No.2. According to the invention, an IbROS1 gene is cloned, a plant overexpression vector of the IbROS1 gene is constructed, and an optimized agrobacterium-mediated method is utilized to transform and cultivate sweet potatoes, so that an IbROS1 overexpressed transgenic sweet potato strain is obtained; phenotypic analysis finds that overexpression of IbROS1 can significantly reduce the methylation level of sweet potato genome DNA, and finds that IbROS1 strongly inhibits formation and expansion of tuberous roots, resulting in sharp drop of root weight, reduction of starch content, increase of lignin content and change of related developmental gene expression profiles; the key negative regulation effect of the IbROS1 in sweet potato root tuber development is disclosed, a new gene resource and theoretical basis are provided for accurately regulating the yield and quality of sweet potatoes through an epigenetic means, and the IbROS1 has an important application prospect in sweet potato molecular breeding.

The invention relates to the technical field of wheat breeding, in particular to a wheat gibberellic disease resistance gene KASP marker and application thereof, the wheat gibberellic disease resistance gene KASP marker mainly comprises DQK009, DQK011, DQK004, DQK008, DQK005, DQK007, DQK001 and DQK002, and the technical problem that at present, high-throughput screening is difficult to conduct on other uncloned genes of wheat is mainly solved. The invention further provides application of the KASP marker in two aspects of wheat genotype detection and rapid and efficient breeding of the gibberellic disease resistant wheat, and the technical problem that the breeding time of the gibberellic disease resistant wheat is long at present is mainly solved.

The invention relates to the technical field of gene engineering, in particular to a nonglutinous rice type gene for cultivating nitrogen-efficient and yield-increasing rice and application of the nonglutinous rice type gene. The indica rice type HTD1 gene is located on a No.4 chromosome of rice, the nucleotide sequence of the indica rice type HTD1 gene is as shown in SEQ ID NO.1, and the amino acid sequence coded by the indica rice type HTD1 gene is as shown in SEQ ID NO.2. The HTD1 gene is cloned from a 9311 indica rice variety, a gene editing carrier is constructed, an indica rice type HTD1 editing plant is obtained, and the indica rice type HTD1 editing plant can regulate and control tillering, yield and nitrogen absorption capacity of rice. The character determination of the edited plant shows that compared with the control group rice, the indica rice type HTD1 edited plant shows the characters of tillering, yield and nitrogen absorption capacity increase under the low-nitrogen and high-nitrogen conditions, which indicates that the indica rice type HTD1 gene plays an important regulation function in the aspects of tillering, yield and nitrogen utilization of rice. An important gene resource is provided for cultivating a new rice variety with high yield and efficient nitrogen utilization, and remarkable agricultural economic value and ecological benefit are achieved.