Coding gene of haemaphysalis qinghaiensis aquaporin (AQP) and application of coding gene

An aquaporin and encoding gene technology, which is applied in the field of the encoding gene of the aquaporin of the blood tick in Qinghai, can solve the problems of little research on AQPs and unclear biological function.

Inactive Publication Date: 2018-10-12
LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
View PDF0 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Ticks bite hosts to transmit pathogens and have a large number of liquid transport activities in their life cycle. Aquaporins (AQPs) are an important functional protein that is widely present on the cell membrane of organisms and specifically me

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0024] Qinghai blood ticks collected from the wild were kept in the Laboratory of Parasite and Arbovirus Innovation Team of Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, cultivated in an artificial climate box (temperature 26±1°C, RH70%, natural light), and raised by rabbits. Carcass bites are filled with blood and passaged.

[0025] According to the AQPs gene family published in the NCBI database, the amino acid sequence of six species of ticks (GenBank accession numbers: FN178519, CAX48964, XM002399751, XP002399794, DQ904355, ABI53034, KP406519, CV443183, AIT69684, KJ626366, CAR661653, FM21ES molecular characteristics) The length of the complete AQPs gene is about 1kb, and there are two amino acid sequence highly conserved regions (NPA) in each complete copy of the AQPs gene. Based on these two regions, we designed amplifying primers to amplify the partial sequence of the AQPs gene of Haemalax qinghaiensis ( AQP-F:5'-TCC CAC CTG AAY CCY GCM...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a coding gene of haemaphysalis qinghaiensis aquaporins (AQPs) and an application of the coding gene. The coding gene of the AQPs can be used for preparing novel anti-tick medicines and anti-tick vaccines. A sequence of the coding gene of the AQPs is shown as SEQ ID NO:1. The invention also provides specific primers cloned by the coding gene of the AQPs, wherein sequences ofthe specific primers are shown as SEQ ID NO:2 and SEQ ID NO:3; the invention also provides a method for cloning the coding gene of the AQPs, and the cloned gene product can be also applied to in-vitro recombinant protein expression and antibody preparation; and an obtained antibody can be used for preparing the novel anti-tick medicines and the anti-tick vaccines. The invention has important theoretical significance an potential application value on prevention and control of ticks. The coding gene of the haemaphysalis qinghaiensis aquaporins (AQPs) and the cloning method of the coding gene provided by the invention are applicable to systematic researches of subtype, varieties, expression distributin, protein structures, biological functions and the like of the AQPs.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to the coding gene of Qinghai blood tick aquaporin (AQP) and its application. Background technique [0002] Ticks are obligate blood-sucking ectoparasites and the second largest biological agent that transmits a variety of human and animal diseases, causing great harm to human health and animal husbandry. The prevention and control of ticks has become the focus of attention and research hotspot at home and abroad in recent years. There are defects such as drug resistance and environmental pollution in the control of ticks by chemical drugs, and new anti-tick preparations have broad application prospects. Ticks bite hosts to transmit pathogens and have a large number of liquid transport activities in their life cycle. Aquaporins (AQPs) are an important functional protein that is widely present on the cell membrane of organisms and specifically mediates liquid transmembrane trans...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12N15/12C12N15/11C12N15/10A61K39/395A61K39/00A61P33/14
CPCA61K39/0003A61K2039/505A61P33/14C07K14/43527
Inventor 牛庆丽刘志杰杨吉飞罗建勋殷宏
Owner LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap