Paddy rice fragile straw controlling gene BC1 and use thereof

A gene and brittle stalk technology, applied in the field of rice BC1 gene, to achieve the effect of increasing content and quality, reducing environmental pollution, and improving material

Inactive Publication Date: 2004-04-14
INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Studies on rice brittle stalk mutants have been reported for a long time, but they are limited to phenotype, physiology and preliminary genetic location.

Method used

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  • Paddy rice fragile straw controlling gene BC1 and use thereof
  • Paddy rice fragile straw controlling gene BC1 and use thereof
  • Paddy rice fragile straw controlling gene BC1 and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] 1. Rice material

[0036] Rice (Oryza sativa ssp.) mutant Brittle Culm1-101, the original wild-type material is

[0037] "Shuangkezao" indica rice variety.

[0038] 2. Analyze and target groups

[0039] The homozygous bc1-101 mutant was crossed with the original wild-type variety C-Bao, and F 1 A total of 30,000 F 2 Individuals, and 7068 bc1-101 mutant individuals were selected as the mapping population. Take about 2 grams of young leaves from each plant at the seedling stage to extract DNA.

[0040] 3. Localization of BC1 gene by SSLP, RFLP, and CAPS markers

[0041] Using the Modified CTAB Method [9] Genomic DNA for gene mapping was extracted from rice leaves. About 100mg of rice leaves were taken, frozen in liquid nitrogen, ground into powder in a small mortar with a diameter of 5cm, transferred to a 1.5ml centrifuge tube to extract DNA, and the obtained DNA precipitate was dissolved in 100μl ultrapure water. 1 μl DNA sample was used for each SSLP and CAPS re...

Embodiment 2

[0048] Embodiment 2 transformed plants (rice is an example)

[0049] nh12 and aa06 are two plasmid clones from a random library used to sequence the BAC clone OSJNBa0036N23, which were joined by a common NheI site into a 5.8 kb (na18) fragment that includes the complete ORF region of BC1. At the same time, na18 was digested with BamHI to generate a 3.3kb fragment, which only contained the ORF of the first half of BC1. The 5.8kb and 3.3kb fragments were respectively cloned into the binary vector pCAMBIA1300, and the plasmids pCna18 and pCna181T for transformation were obtained (Fig. 5). These two plasmids were transformed into Agrobacterium tumefaciens strain LBA4404 by electric shock method to transform rice. Mature seeds of the bc1-101 mutant were dehulled and sterilized, and inoculated into a medium for inducing callus. After 3 weeks of culture, the callus was grown from the scutellum, and the vigorously growing, light yellow, relatively loose embryogenic callus was select...

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Abstract

The invention clones and identifies the gene of control rice stem fragility from rice brisk stem brittle culml(bcl), the gene is named as BCl. Transgenics function complementary experiment testifies that BCl has the function of control rice stem mechanism intensity. The gene series analysis testifies that the protein has 60.7 homologisation with the COBRA protein encoded by COB. The observation and the physical standards also testify that the cloned gene has function of adjusting the thickness of the plant cell wall and the supporting quality of plant stem.

Description

technical field [0001] The invention belongs to the field of plant genetic engineering. Specifically, the present invention relates to a rice BC1 (Brittle Culm) gene isolated by map-based cloning technology, and the function of the gene identified by a transgene complementation experiment; it also relates to a gene containing the gene and homologous sequences with similar functions Genes and related vectors and transformation methods; the present invention also relates to the use of the gene or its functional analogs to regulate the thickness of plant cell walls, mainly to change the content and components of some secondary metabolites such as cellulose, lignin or hemicellulose in the cell walls, Thereby increasing or reducing the mechanical strength and hardness of crop stems, leaves and leaf sheaths to improve the lodging resistance, disease resistance, insect resistance and stress resistance of crops; and using the gene to increase the cellulose content and It can also imp...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/415C12N15/29C12N15/82
CPCC07K14/415C12N15/8261Y02A40/146
Inventor 李家洋钱前李云海曾大力藤胜
Owner INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI
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